SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Hua Zhang,Linlin Xu,Yue Tian,Anxin Jiao,Ming Chen
Optical anticounterfeiting labels based on surface-enhanced Raman scattering (SERS) with enriched molecular “fingerprint” information is not only related to the enhanced sensitive detection but also strongly dependent on excellent long-time stability of nano-platforms. By virtue of in-situ overgrowth of highly dense and close-packed Ag nanoparticles (NPs) on Ti 3 C 2 T x nanosheets, herein, we prepared an extraordinary SERS active nano-substrates with exceptional long-term stability for achieving ultrastable anticounterfeiting tags. The obtained Ag@Ti 3 C 2 T x exhibit a significantly SERS activity with a detection limit of methylene blue (MB) molecules as low at 10 -16 M, approaching 0.1 femtomole (fM) ultra-trace level. More importantly, since nearly all active Ag NPs were completely encapsulated in the 2D supports, the unparalleled stability of the resultant Ag@Ti 3 C 2 T x can be undoubtedly verified by immersing them into H 2 O 2 oxidative solution, which is obviously better than that of bare Ag NPs. Then, the hybrid Ag@Ti 3 C 2 T x were subsequently incorporated into polydimethylsiloxane (PDMS) to obtain transparent flexible Ag@Ti 3 C 2 T x @PDMS, giving rise to the ultra-sensitive SERS imaging-based steganographic information. Interestingly, the ultrastable anticounterfeiting capability of the smart SERS nano-platforms is further highlighted by keeping them even after for 400 days storage. The present work holds a great potential for propelling robust SERS-based steganography in many practical real-world scenarios.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Xiang-Cheng Lin,Jian Liu,Miaomiao Hu,Lingjun Song,Minzhe Li,Qinjie Kou,Rong Huang,Lixian Sun,Changchun Wen
The abnormal fluctuations of glutathione (GSH) in vivo often reflect disease progression and are closely linked to human metabolism and physiological functions. Highly sensitive and selective detection of GSH is crucial for guiding early diagnosis and treatment; however, achieving this remains a significant challenge. In this study, we developed an enzyme activity sensor platform for the efficient detection of GSH. This platform utilizes Au@CuS core–shell materials loaded with CuO 2 nanoparticles to create a composite nanosensor system. Under slightly acidic conditions, CuO 2 on the nanomaterial’s surface decomposes into H 2 O 2 and Cu 2+ ions. The generated H 2 O 2 then reacts with tetramethylbenzidine (TMB) in the presence of peroxidase-like CuS to yield oxidized tetramethylbiphenyl ( OX TMB), which generates a distinctive Raman signal. Upon addition of GSH to the system, the unique OX TMB signal diminishes due to GSH’s strong antioxidant capacity and the consequent consumption of OX TMB. This sensing method enables sensitive detection of GSH, with a detection limit as low as 1.2 × 10 -13 mol∙L -1 . This approach holds promise for providing researchers with rapid and precise in vitro analysis of GSH, serving as an indicator for early disease diagnosis and real-time evaluation of treatment efficacy.
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TALANTA
·
2012
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IF 3.794
Shenfei Zong,Zhuyuan Wang,Jing Yang,Chunlei Wang,Shuhong Xu,Yiping Cui
We report a dual mode cancer cell targeting probe based on CdTe quantum dots (QDs) conjugated, silica coated [email protected] core–shell nanorods ( [email protected] NRs), which can generate both surface enhanced Raman scattering (SERS) and fluorescence signals. In such a probe, folic acid (FA) is used as a targeting ligand for folate receptors (FRs) overexpressed cancer cells. To synthesize the probe, [email protected] NRs were first prepared to serve as the SERS substrates by coating an Ag shell on the gold nanorods. Then the [email protected] NRs were labeled with 4-mercaptobenzoic acid (4MBA) to generate SERS signals, followed by being coated with a silica shell through a modified Stöber method. Finally, CdTe QDs and FA were conjugated to the silica coated [email protected] NRs by the carbodiimide chemistry to yield fluorescence and the targeting ability, respectively. To validate the targeting capability of the probe, in vitro experiments were conducted, using HeLa cells with overexpressed FRs as the model target cells and MRC-5 cells with a low folate receptor expression level as the negative control. Both the fluorescence imaging and the SERS mapping results confirmed that the proposed probe can be used as an efficient cancer cell targeting agent. This kind of multifunctional probe has great potential in the diagnosis and therapeutics of cancerous diseases due to its specific targeting and multiplex imaging abilities, especially in the simultaneous tracking of multiple components in a hybrid bio-system.
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Journal of Materials Chemistry C
·
2015
·Vol. 3
·期 39
·10154-10163
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IF 4.696
Kaisheng Yao,Xinying Li,Yuling Zhao,Weiwei Lu,Jianji Wang,Jiongliang Yuan
In this work, a simple and facile strategy was developed for the fabrication of large sized Au spherical architectures (AuSAs) with flexible nanoflakes as subunits by using a liquid–liquid interfacial reaction route at room temperature. The as-prepared AuSAs were characterized and analyzed by scanning electron microscopy, X-ray powder diffraction, thermogravimetric analysis and transmission electron microscopy. The effects of a series of factors on the morphologies and structures of the products were studied in detail. On the basis of control experiments and molecular dynamics simulations, a possible growth mechanism was suggested for the formation of AuSAs at the interface between toluene and water. Furthermore, due to the relatively large particle size, high surface roughness and high symmetry of the spherical characteristics, AuSAs could be used to perform individual particle SERS investigation, which demonstrated remarkable SERS responses and high reproducibility.
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Food Analytical Methods
·
2022
·Vol. 15
·期 8
·2346-2356
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IF 3.498
Agyekum Akwasi Akomeah,Kutsanedzie Felix Y. H.,Mintah Benjamin Kumah,Annavaram Viswadevarayalu,Braimah Alberta Osei
Norfloxacin is an antibiotic in the fluoroquinolone family licenced for use in animals. However, residues in animal products can have negative consequences for consumers. As a result, residue detection in various food matrices must be considered. Norfloxacin accumulates in animal-derived foods, causing deleterious consequences in humans such as foetal deformity, renal failure and drug resistance. A built-in SERS-Au@Ag nanosensor coupled with GA-PLS was used to rapidly detect norfloxacin in the specimen of the spiked fish muscles due to the threat to human lives. A detection limit of 2.36 × 10 −5 μg/mL was realized in the spiked fish muscle sample for norfloxacin compared to the European Commission’s maximum threshold level of 100 μg/kg, indicating the sensor’s ability to detect and quantify norfloxacin at a relatively lower level. The recovery rates (RC) and coefficient of variation (CV) measured in the spiked fish muscle samples for norfloxacin analytes and their standard solutions were between 99.70–105.00% and 0.17–5.21%, respectively. The low CV values imply the reproducibility of the obtained data. The constructed model recorded residual predictive deviations (RPD) greater than three (3), demonstrating the robustness and resilience of the developed genetic algorithm-partial least squares (GA-PLS) model. GA-PLS-built models predicted all results within 4.07 s, which indicates the nanosensor’s ability to rapidly detect norfloxacin in fish to guarantee safety and public health. The SERS probe holds promise for rapid quantification of norfloxacin at microgram per milliliter level in fish to guarantee safety in commerce. Graphical abstract
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Qian Zhang,Xiang-Dong Wang,Ting Tian,Li-Qiang Chu
Surface-enhanced Raman scattering (SERS) sensors have been extensively studied for ultrasensitive detection of diverse chemical or biological analytes. Facile fabrication of highly sensitive SERS substrates is believed to be of crucial importance in these analytical applications. In this regard, the preparation of 3-dimensional (3D) SERS substrates are explored via the incorporation of multilayered silver nanoparticles (AgNPs) into poly (oligo(ethylene glycol) methacrylate) (POEGMA) brushes by repeating the immersion-rinsing-drying steps for different lengths of time (i.e., the so-called in-stacking method). The POEGMA brushes of different chain lengths are synthesized by surface-initiated atom transfer radical polymerization (ATRP) with various reaction time. The resulting POEGMA/AgNP nanocomposites are characterized by FE-SEM, UV–vis and Raman spectroscopy. FE-SEM and UV–vis results indicate that the AgNPs are successfully incorporated into the POEGMA brushes with a 3D configuration. The nanocomposite films are employed as SERS substrates for the detection of a Raman reporter molecule (i.e., 4-aminothiophenol), giving rise to an enhancement factor of up to 1.29 × 10 7 and also having relatively good uniformity and reproducibility. The obtained 3D SERS substrates are also used for the detection of a typical gram-positive bacterium, Staphylococcus aureus . The limit of detection is found to be as low as ca. 8 CFU/mL.
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ACS Applied Materials & Interfaces
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2020
·
IF 8.758
Yu Han,Si-Rong Wu,Xiang-Dong Tian,Yun Zhang
Three-dimensional (3D) plasmonic nanostructures are emerging as excellent surface-enhanced Raman spectroscopy (SERS) substrates for chemical and biomedical applications. However, the correlation of 3D (including both in-plane and out-of-plane) plasmonic coupling with the SERS properties to deepen the understanding of 3D SERS substrates remains a challenge. Here, we perform correlation studies of 3D plasmonic coupling and SERS properties of the 3D hierarchical SERS substrates by tuning the multiscale structural elements. The effects of zero-dimensional (0D; the size of the building blocks), one-dimensional (1D; the thickness of the 3D substrates), and two-dimensional (2D; the composition of individual monolayers) structural elements on 3D plasmonic coupling are studied by performing UV–vis–near-infrared (NIR) spectroscopy and measuring SERS performance. It shows that both the extinction spectra and SERS enhancement are tuned at the 3D structural level. It is demonstrated that the plasmonic resonance wavelength (PRW) stemming from the 3D plasmonic coupling correlates with the SERS averaged surface enhancement factor (ASEF) and is improved by more than tenfold at the optimum 3D nanostructure. The optimized substrate is used to quantitatively analyze two small biological molecules. Moreover, as a proof-of-concept study, the substrate is first applied to differentiate between living liver normal and cancer cells with a high prediction accuracy through the spectral features of the cell membranes and the metabolites secreted outside the cells. We expect that the tuning of plasmonic coupling at the 3D level can open up new routes to design high-performance SERS substrates for wide applications.
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TALANTA
·
2025
·
IF 6.1
Haonan Wang,Qingling Nie,Luyao Lin,Ruiyun You,Ziting Ye,Jin Zhang,Yudong Lu,Zhenwen Lin,Zixing Yan
Recent years have witnessed remarkable progress in cellulose and hydrogels, facilitating the development of wearable green sensing technology. This study presents a novel flexible hydrogel patch designed as a skin-adhesive platform for wearable sensors, synthesized through the strategic integration of hydrogel and porous “sponge-like” technologies. Fabricated from acrylamide and sodium alginate matrices enhanced with tannic acid modification, the composite exhibits superior mechanical performance, biocompatibility (98.75 % cell viability), antimicrobial efficacy (92.03 % inhibition rate against S. aureus ), and tissue adhesion. A sulfonated cellulose nanofibril/sodium alginate/silver nanoparticle (S–CNF/SA/Ag NPs) composite membrane was engineered as a surface-enhanced Raman spectroscopy (SERS) detection module, achieving remarkable sensitivity for sweat biomarkers with detection limits of 10 −6 M for urea and 10 −5 M for lactic acid. Experimental validation confirms exceptional epidermal conformability and sustained adhesion. This technological advancement establishes a foundation for transformative applications in personalized healthcare monitoring, exercise physiology assessment, clinical diagnostics, and preventive medicine, thereby accelerating innovation in wearable biosensing platforms.
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CHEMOSPHERE
·
2021
·
IF 7.086
Fugang Xu,Wenjuan Shang,Mengren Xuan,Guangran Ma,Zixiang Ben
A SERS substrate FP/Ag/ZIF-8 composed of filter paper (FP), silver nanoparticles (AgNPs) and zeolitic imidazolate framework (ZIF-8) film arranged in a layered structure was developed for sensitive detection of pesticide thiram in various samples. Roles of these components in analyte adsorption and Raman signal enhancement were studied using a pesticide intermediate 4-Aminothiophenol (4-ATP) as the probe. The substrate showed high adsorption and optimized SERS response with thick metal organic framework (MOF) coating (125 nm), which is different from previous reported plasmonic particle-MOF composite substrate, where thinnest MOF coating produced the strongest SERS signal. Detection limit for 4-ATP improved 1000-fold on FP/Ag/ZIF-8 (3 pM) compared with that on FP/Ag (3 nM). Importantly, the FP/Ag/ZIF-8 with porous and flexible property can efficiently capture pesticide thiram in different real samples using soaking, filtration or swabbing operation. The subsequent SERS detection of thiram showed advantages of low detection limit (soaking, LOD: 0.04 nM in lake water), fast detection (filtration, within 1 min in peach juice) and suitable for curve surface analysis (swabbing, LOD: 0.1 ng/cm 2 on apple peel), respectively. The substrate also displayed good reproducibility, high stability and size-selective response for thiram detection. Such a layered plasmonic particle/MOF hybrid may hold great promise for toxicant analysis in environment and food.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jingying Yang,Mingfei Pan,Kaixin Liu,Xiaoqian Xie,Shan Wang,Liping Hong,Shuo Wang
A core-shell AuNRs@Ag-enhanced and magnetic separation-assisted surface-enhanced Raman spectroscopy (SERS) immunosensing platform was developed for rapid, accurate and sensitive detection of amantadine (AMD) contaminant in animal-derived foods. This SERS immunosensing platform works in a competitive strategy, which used the 4-mercaptobenzoic acid (4-MBA) labeled AuNRs@Ag with AMD-BSA immobilized as a signal probe and magnetic beads (MBs) with mAbs covalently attached to the surface as a capture probe. It was found that the synthesized core-shell AuNRs@Ag could significantly enhance the SERS signal of the reporter 4-MBA ( EF : 2.91 × 10 7 ), and the coating of Ag shell effectively avoided the shedding of 4-MBA, thereby improving the sensitivity and stability of SERS analysis. The use of MBs not only overcomes the shortcoming of mAbs inactivation, but also simplifies the detection procedure, improving the detection efficiency. This SERS immunosensing platform was demonstrated to have a linear response in a wide AMD concentration range of 0.01–50 μg/L with high sensitivity (limit of detection (LOD): 0.0038 μg/L) and specificity. For actual animal-derived foods (chicken, milk, egg), acceptable recoveries of AMD (82.0–106.0%) at three spiked levels (0.5, 5, 50 μg/kg) were obtained with relative standard deviation (RSD, n = 5) ranging from 4.7% to 9.6%. The whole detection procedure can be completed within 30 min. These results demonstrated that the proposed SERS immunosensing platform was reliable and sensitive in the quantitative analysis of AMD and could be applied as an effective tool for rapid screening for AMD-contaminated foods.
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TALANTA
·
2024
·
IF 5.6
Lichen Gou,Ling Li,Siqi Wei,Yunfei Tian,Xiandeng Hou,Li Wu
Herein, the surface-enhanced Raman scattering (SERS) platform was combined with an azo coupling reaction and an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes for the detection of histamine. The praseodymium oxide on aluminum alloy was successfully synthesized by the rare-earth-salt-solution boiling bath method and modified by stearic acid. Its surface exhibits a water contact angle (WCA) of 125.0°. Through the azo derivatization reaction with 3-amino-5-mercapto-1,2,4-triazole (AMTA) diazonium salts, histamine can be converted into the derivatization product with higher Raman activity. The mixture of the derivatization product and β-cyclodextrin-modified Ag nanoparticles (β-CD-AgNPs) were dropped onto the surface of an aluminum alloy covered with a hydrophobic layer of praseodymium oxide and stearic acid complexes, and dried for SERS measurement. The intensity ratio between the SERS peaks at 1246 cm −1 and 1104 cm −1 (I 1246 /I 1104 ) of the derivatization product was used for the quantification of histamine. Under the selected conditions, the limit of detection (LOD) and the limit of quantification (LOQ) for this method were 7.2 nM (S/N = 3) and 24 nM (S/N = 10), respectively. The relative standard deviation (RSD) of this method for the determination of 1 μM histamine was 6.1 % (n = 20). The method was also successfully used for the determination of histamine in fish samples with recoveries ranging from 92 % to 111 %. The present method is simple, sensitive, reliable, and may provide a new approach for preparing the composite hydrophobic layer that can enhance SERS signals through hydrophobic condensation effect. Meanwhile, it may have a promising future in the determination of small molecular compounds containing an imidazole ring.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Luo Xianzhun,Dai Qingshan,Qiu Xia,Wang Dongmei,Li Yongxin
As biomarkers of cancer, the accurate and sensitive detection of microRNAs is of great significance. Therefore, we proposed a surface-enhanced Raman scattering (SERS)/electrochemical (EC) dual-mode nanosensor for sensitively detecting miRNA-141. The nanosensor uses Au@Ag nanowires as a novel SERS/EC sensing platform, which has the advantages of good biocompatibility, fast response, and high sensitivity. The dual-mode nanosensor can not only effectively overcome the problem of insufficient reliability of single signal, but also realize the amplification and stable output of the detection signal, to ensure the reliability and repeatability of miRNA detection. With this sensing strategy, the target miRNA-141 can be detected over a wide linear range (100 fM to 50 nM) (LOD of 18.4 fM for SERS and 16.0 fM for electrochemical methods). In addition, the process shows good selectivity and can distinguish miRNA-141 from other interfering miRNAs. The actual analysis of human serum samples also proves that our strategy has good reliability, repeatability, and has broad application prospects in the field of analysis and detection. Graphical Abstract
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危险品化学品经营许可证（带存储）