ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2023
·Vol. 30
·期 16
·47873-47881
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IF 5.8
Yan Boyin,Han Chunyu,Liu Zhiquan,Wu Guizhi,Wang Songxue,Li Jincheng,Xia Wenxiang,Cui Fuyi
Abstract β- N -methylamino- l -alanine (BMAA), which has been considered as an environmental factor that caused amyotrophic lateral sclerosis/parkinsonism-dementia complex (ALS/PDC) or Alzheimer’s disease, could be produced by a variety of genera cyanobacteria. BMAA is widely present in water sources contaminated by cyanobacteria and may threaten human health through drinking water. Although oxidants commonly used in drinking water plants such as chlorine, ozone, hydrogen peroxide, and hydroxyl radicals have been shown to effectively degrade BMAA, there are limited studies on the mechanism of BMAA degradation by different oxidants, especially ozone. This work systematically explored the effectiveness of BMAA ozonation degradation, investigated the effect of the operating parameters on the effectiveness of degradation, and speculated on the pathways of BMAA decomposition. The results showed that BMAA could be quickly eliminated by ozone, and the removal rates of BMAA were nearly 100% in pure water, but the removal rates were reduced in actual water. BMAA was primarily degraded by direct oxidation of ozone molecules in acidic and near-neutral conditions, and indirect oxidation of •OH accounted for the main part under strong alkaline conditions. The pH value had a significant effect on the decomposition of BMAA, and the degradation rate of BMAA was fastest at near-neutral pH value. The degradation rates of TOC were significantly lower than that of BMAA, indicating that by-products were generated during the degradation process. Three by-products ([M-H] + = 105, 90, and 88) were identified by UPLC-MS/MS, and the degradation pathways of BMAA were proposed. The production of by-products was attributed to the fracture of the C–N bonds. This work is helpful for the in-depth understanding on the mechanism and demonstration of the feasibility of the oxidation of BMAA by the ozone process. Highlights • The reaction of ozonation BMAA was easy to occur. • The degradation rate was fast under near-neutral conditions. • Direct oxidation under neural conditions was the main pathway for ozone degradation of BMAA. • Three products were detected, and the reaction path was inferred. Graphical Abstract
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JOURNAL OF CHROMATOGRAPHY A
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2023
·
IF 4.1
Yuhan Ning,Yang Xu,Jingyi Bao,Weiping Wang,Ai-jun Wang
In this work, β-cyclodextrin-functionalized magnetic graphene oxide (NiFe 2 O 4 @GO@β-CD) was synthesized and employed as magnetic solid-phase extraction adsorbent for the extraction of bisphenols before high performance liquid chromatography analysis. The modification of β-cyclodextrin could enhance the adsorption performance of NiFe 2 O 4 @GO@β-CD towards bisphenols through the host-guest interaction and hydrogen-bond interaction. Under the optimal conditions, good linearities between peak area and concentration of bisphenols (1 – 300 μg L −1 , r ≥ 0.9989) were obtained with the limits of detection (S/ N = 3) in the range of 0.050 – 0.10 μg L –1 . The recoveries of bisphenols in milk and milk packaging ranged from 78.0% to 101.6%. Moreover, NiFe 2 O 4 @GO@β-CD showed stable chemical properties and good reusability with the recoveries of bisphenols remained above 80.0% after 12 MSPE cycles. The adsorption characteristics of NiFe 2 O 4 @GO@β-CD towards bisphenols fitted well with the pseudo-second-order kinetic model and Langmuir model. The hydrogen-bond interaction, π-π interaction, host-guest interaction and electrostatic interaction between sorbent and bisphenols played important role during the adsorption process. The developed method showed potential applications for the analysis of trace bisphenols in milk and milk packaging.
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International Journal of Bioprinting
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2023
·
IF 8.4
Hu Xulin,Li Hu,Qiao Liang,Yang Shuhao,Wu Haoming,Peng Chao,Zhang Yamei,Lan Hai,Yang Hua,Li Kainan
Human bone is composed of cortical bone and cancellous bone. The interior portion of natural bone is cancellous with a porosity of 50%-90%, but the outer layer is made of dense cortical bone, of which porosity was not higher than 10%. Porous ceramics were expected to be research hotspot in bone tissue engineering by virtue of their similarity to the mineral constituent and physiological structure of human bone. However, it is challenging to utilize conventional manufacturing methods to fabricate porous structures with precise shapes and pore sizes. Three-dimensional (3D) printing of ceramics is currently the latest research trend because it has many advantages in the fabrication of porous scaffolds, which can meet the requirements of cancellous bone strength, arbitrarily complex shapes, and individualized design. In this study, β-tricalcium phosphate (β-TCP)/titanium dioxide (TiO 2 ) porous ceramics scaffolds were fabricated by 3D gel-printing sintering for the first time. The chemical constituent, microstructure, and mechanical properties of the 3D-printed scaffolds were characterized. After sintering, a uniform porous structure with appropriate porosity and pore sizes was observed. Besides, biological mineralization activity and biocompatibility were evaluated by in vitro cell assay. The results demonstrated that the incorporation of TiO 2 (5 wt%) significantly improved the compressive strength of the scaffolds, with an increase of 283%. Additionally, the in vitro results showed that the β-TCP/TiO 2 scaffold had no toxicity. Meanwhile, the adhesion and proliferation of MC3T3-E1 cells on scaffolds were desirable, revealing that the β-TCP/TiO 2 scaffolds can be used as a promising candidate for repair scaffolding in orthopedics and traumatology.
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CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Jie Jia,Yinli Liu,Dequn Wu,Jianyong Yu,Tingting Gao,Faxue Li
Water contamination caused by the discharge of textile dyeing wastewater is a thorny issue that cannot be ignored nowadays. In this work, eco-friendly β -cyclodextrin based nanocubes (cl-CD@CA) with low cost and exceptional adsorption capacity were fabricated by a facile method to treat textile dyeing effluents. The prepared adsorbents showed high water insolubility and extraordinary adsorption capacity towards methylene blue, crystal violet, neutral red, Pb 2+ , Cd 2+ , and Sb 3+ ions with corresponding maximum adsorption capacity of 1523.6, 1012.2, 419.2, 758.7, 525.3, and 398.1 mg/g, much higher than that of some other adsorbents. The adsorption kinetic and isotherm studies indicated that the adsorption process of cl-CD@CA fit pseudo-second-order adsorption kinetic model and Langmuir isotherm model well, manifesting its monolayer chemisorption. Remarkably, the cl-CD@CA, presenting high selective adsorption toward cationic rather than anionic dyes, was potential for mixed dye separation. Moreover, the cl-CD@CA maintained excellent recyclability after 10 regeneration cycles. Excitingly, cl-CD@CA exhibited high static and dynamic adsorption capacity for actual dyeing wastewater, with a 79.3 % reduction in COD and 95 %, 29 % and 16 % concentration reduction of Pb 2+ , Cd 2+ and Sb 3+ . Therefore, the newly developed lost-cost yet high-efficiency cl-CD@CA can serve as promising adsorbents to alleviate the water pollution caused by dyeing wastewater.
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Materials Today Communications
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2022
·
IF 3.662
Jianhua Lv,Yaru Fang,Mi Wu,Xiaoyu Ou,Wenchang Zhang,Shiyu Wang,Huaiguo Li,Lei Shang,Mingfeng He,Yan Zhao
Microsized β-chitin fibers were prepared, and PAAm /β-chitin composited hydrogels were synthesized by situ free radical polymerization . The morphology, mechanical properties, swelling ratio, transparency and drug release capabilities of the composited PAAm hydrogels were studied systematically. The results showed that the microsized β-chitin fibers were evenly dispersed in the PAAm hydrogels, and the tensile and compressive strength of PAAm hydrogels were increased by 2.1 folds and 4.4 folds respectively by adding with 15 phr microsized β-chitin fibers, while water absorbency and transparency of the hydrogels were decreased with higher microsized β-chitin fibers content. The drug release behaviors of diclofenac sodium in the hydrogel followed the Fickian diffusion mechanism with n value of 0.099 and 0.147 for PAAm hydrogels and PAAm/β-chitin composite hydrogels respectively, and the release time of 60 % drug were extended from 1 h to 5 h by the addition of 5 phr microsized β-chitin fibers. The results indicate that microsized β-chitin fibers can improve the mechanical properties and decrease the drug release rate of PAAm hydrogel, and the composited hydrogels may find potential applications in the field of transdermal drug delivery system.
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RUSSIAN JOURNAL OF APPLIED CHEMISTRY
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2023
·Vol. 95
·期 7
·1036-1047
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IF 0.9
Yu Xiaoxia,Zhou Qi,Bi Lihua
Abstract In this work, we firstly used the most common, easily available and inexpensive β-cyclodextrin (β-CD) to construct a sensitive electrochemical sensor, with the hybrid components of polyaniline (PANI) and phosphomolybdic acid (PMo 12 ), used for the detection of ascorbic acid (AA). First, β-CD was electropolymerized on indium tin oxide (ITO) to form the modified electrode β-CD/ITO, and then PANI and PMo 12 were co-polymerized on the film of β-CD to fabricate a novel electrode PMo 12 -PANI/β-CD/ITO. The experimental parameters for the construction of the modified electrode were studied and the optimized conditions were gained. Compared to the electrode ITO, β-CD/ITO, PANI/ITO, PMo 12 -PANI/ITO, the developed electrode PMo 12 -PANI/β-CD/ITO shows high sensitivity for the electrochemical detection of AA, giving a low detection limit of 15 nM and wide linear concentration range from 0.01 to 3000 μM. Further, the developed electrode was used to detect AA in real samples showing excellent accuracy and reproducibility, confirming good practicability, convenience and cost-effective.
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CHEMICO-BIOLOGICAL INTERACTIONS
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2023
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IF 5.1
Yue Gao,Shicheng Fan,Pengfei Zhao,Huilin Li,Chenghui Cai,Xuan Li,Yanying Zhou,Min Huang,Huichang Bi
Liver fibrosis can be characterized by the over-deposition of extracellular matrix (ECM). It has been reported that β-catenin/TCF4 interaction was enhanced in bile duct ligation (BDL) model, which implicated the critical role of β-catenin/TCF4 interaction during the progression of fibrosis . However, whether inhibiting β-catenin/TCF4 signaling attenuates liver fibrosis remains unknown. In the current study, we used ICG-001, an inhibitor that disrupts the interaction between CREB binding protein (CBP) and β-catenin, to inhibit β-catenin/TCF4 transcriptional activity. We also used LF3, a small molecule antagonist, to inhibit β-catenin/TCF4 interaction. The antifibrotic effect of ICG-001 and LF3 was assessed on BDL-induced liver fibrosis model. The results indicated both ICG-001 and LF3 significantly reduced the positive staining area of Sirius Red and α-SMA. The protein expression levels of α-SMA, Collagen Ⅰ and CD31 were also significantly downregulated in BDL + ICG-001 and BDL + LF3 groups. Besides, ICG-001 and LF3 promoted portal angiogenesis and inhibited sinusoids capillarization in fibrotic livers. For mechanistic study, we measured the level of leukocyte cell-derived chemotaxin 2 (LECT2), a direct target of β-catenin/TCF4, which was recently reported to participate in hepatic fibrosis by regulating angiogenesis. The results showed that both ICG-001 and LF3 reduced LECT2 expression in BDL mice. LF3 also downregulated pSer 675 β-catenin and nuclear β-catenin. In conclusion, this study demonstrated that inhibiting β-catenin/TCF4 signaling by ICG-001 or LF3 mitigated liver fibrosis by downregulating LECT2, promoting portal angiogenesis and inhibiting sinusoids capillarization, which provided new evidence that β-catenin/TCF4 signaling might be a target for the treatment of liver fibrosis.
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JOURNAL OF MOLECULAR LIQUIDS
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2023
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IF 6
Ganggang Chen,Yuxin Liu,Baomin Fan,Zining Liu,Jingmao Zhao,Zongqiang Fu
Molecular assembly based on cyclodextrins can realize the aqueous utilization of inherently-insoluble corrosion inhibitors for metals; however, the associated protection mechanism is not yet fully understood. Herein, hydrophobic benzothiazole (BT) was assembled with β-cyclodextrin (β-CD) forming the complex (CD-BT) for protecting copper in the simulated seawater (SSW). Supramolecular complexing was optimized through response surface methodology, and the host–guest interaction was confirmed by spectral, thermal and morphological examinations besides the disclosed assembling thermodynamics. Electrochemical and surface analyses indicated that CD-BT effectively suppressed copper dissolution in SSW yielding a maximum inhibition efficiency of 97.0%. Density functional theory (DFT) calculations were deployed to clarify the accurate complexing behavior of BT inward β-CD's cavity. Benefitting from the favorable incorporating configuration of BT and its overwhelming adsorption energy over the assembling counterpart, CD-BT underwent an incubation–guest release–adsorption process at the copper/SSW interface. Adsorption of released BT molecules and its chelation ability towards cuprous/cupric species accounted for the efficient protection of copper in SSW.
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FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Zhifen Deng,Yuting Xia,Lang Chen,Yi Zhao,Ruihong Wang,Guizhao Liang
β-lactoglobulin (β-LG) is an essential nutrient in milk, but it is the primary allergen causing dairy allergy in humans. Currently, researchers are focusing on using flavonoids to covalently modify β-LG for improving its functionality. However, the impact and underlying mechanisms of rutin covalent modification on the functional properties and allergenicity of β-LG remain unclear. Here, we aim to investigate the changes in allergenicity, digestive characteristics, and antioxidant properties of β-LG after covalent modification using a combination of spectroscopy, enzyme-linked immunosorbent assay (ELISA), simulated digestion, and antioxidant assays. The results indicate that rutin forms covalent bonds with the free amino group, sulfhydryl group, and tryptophan of β-LG, leading to alterations in the secondary structure of β-LG. Furthermore, the modified β-LG exhibits improved antioxidant capacity and decreased allergenicity, along with reduced resistance to pancreatin digestion in vitro . This study provides novel insights and strategies to expand the functional application of β-LG.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Zhen Qin,Sainan Yu,Kemin Zhang,Xiasen Wei,Junjie Li,Zheyi Zhang,Sibao Wan,Haiyan Gao
β-1,3-Glucan-degrading enzymes are widely used in fields such as food processing, plant protection, and breweries. In this work, we identified a glycoside hydrolase (GH) family 157 endo-β-1,3-glucanase (BsGlc157A) from Bacteroides sp. M27 and characterized its biochemical properties, structural model, and antifungal activity. Enzymological characterization indicated that BsGlc157A performs its optimal catalytic activity at pH 6.0 and 40 °C. BsGlc157A adopted the classic (β/α)8 TIM-barrel structure. Two catalytic residues, the nucleophile (Glu215) and the proton donor (Glu123), were confirmed via structural modeling and site-directed mutagenesis. Moreover, BsGlc157A hydrolyzed curdlan into a series of oligosaccharides with polymerization degrees 2–5 and exhibited inhibitory effects on the hyphal growth of typical fruit pathogenic fungi (Monilinia fructicola, Alternaria alternata, and Colletotrichum gloeosporioides), thereby illustrating effective biocontrol activity. These results revealed the catalytic properties and the application potential of GH family 157 β-1,3-glucanase, thus providing valuable biochemistry information about the group of carbohydrate-active enzymes.
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BIOMEDICAL MICRODEVICES
·
2023
·Vol. 25
·期 3
·1-9
·
IF 2.8
Cao Xiaona,Hu Xiaoping,Qiu Ziyi,Xu Ting,Yu Zhenhua,Li Zhe,Jin Huawei,Xu Bingzhe
Early diagnosis of Alzheimer’s disease (AD) is critical for preventing disease progression, however, the diagnosis of AD remains challenging for most patients due to limitations of current sensing technologies. A common pathological feature found in AD-affected brains is the accumulation of Amyloid-β (Aβ) polypeptides, which lead to neurofibrillary tangles and neuroinflammatory plaques. Here, we developed a portable ultrasensitive FET biosensor chip based on a self-assembled nanoporous membrane for ultrasensitive detection of Aβ protein in complex environments. The microscale semiconductor channel was covered with a self-assembled organic nanoporous membrane modified by antibody molecules to pick up and amplify the Aβ protein signal. The nanoporous structure helps protect the sensitive channel from non-target proteins and improves its stability since no chemical functionalization process involved, largely reduces background noise of the sensing platform. When a bio-gated target is captured, the doping state of the polymer bulk could be tuned and amplified the strength of the weak signal, achieving ultrasensitive detecting performance (enabling the device to detect target protein less than 1 fg/ml in 1 µl sample). Moreover, the device simplifies the circuit connection by integrating all the connections on a 2 cm × 2 cm chip, avoiding expensive and complex manufacturing processes, and makes it usable for portable prognosis. We believe that this ultrasensitive, portable, low-cost Aβ sensor chip shows the great potential in the early diagnosis of AD and large-scale population screening applications.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Chun Feng,Lijuan Zhu,Yaqiong Cao,Yin Di,Zongxue Yu,Guhui Gao
This study reports a new strategy for enhancing the anti-corrosion properties of epoxy coatings. For this purpose, beta-cyclodextrin-graphene oxide (β-CD-g-GO) nanosheets were synthesized via anchoring beta-cyclodextrin on a graphene oxide surface in the presence of 3-glycidoxypropyltrimethoxysilane (KH560). The structure of the material was characterized by SEM, EDX, XRD, FT-IR and TGA. The inclusion of 2 wt. % GO and β-CD-g-GO nanosheets in epoxy resin remarkably enhanced the corrosion protection performance of the material relative to that of pure epoxy coating. Electrochemical impedance spectroscopy (EIS) measurements revealed that the impedance of the epoxy coating was improved after the addition of 2 wt. % GO and β-CD-g-GO nanosheets. The most pronounced improvement in the properties of the epoxy coating was obtained using β-CD-g-GO nanosheets. Scratches tests confirmed the reduced steel substrate corrosion in the case of epoxy coating that included β-CD-g-GO nanosheets compared to the corrosion obtained for other samples.
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