Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 9
·2264-2277
·
IF 3.641
Du Lidong,Zhang Ruoyu,Zhao Lei,Tang Shaojian,Hou Zhaohua,Xue Peng
Aim: To assess the anticancer activity of nanoparticles synthesized via a green method using American ginseng (AG). Methods: Stem-leaf saponins from AG (SAG) and heat-transformed stem-leaf saponins from AG (TSAG) were used to synthesize different SAG nanoparticles (SAG-NPs)
and TSAG nanoparticles (TSAG-NPs). The NPs were characterized, and their anticancer activity was assessed in vitro. Results: The NPs, which differed in size (16.69 nm∼253.8 nm), were spherical or polyhedral with a low PDI and good stability. The TSAG-NPs inhibited cancer
cells by inhibiting proliferation, promoting cancer cell apoptosis and directly leading cancer cells to necrosis. The small cell lung cancer cell line (SCLC) NCI-H446 was the most sensitive to the TSAG-AgNPs, with an IC50 value of 20.71±2.38 μg/mL, and the TSAG-AgNPs
inhibited invasiveness and reduced the risk of metastasis. Conclusion: TSAG-AgNPs, selected from many SAG-NPs and TSAG-NPs, are sensitive to SCLC and provide a new approach to the currently limited treatment.
✓ 已验证
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2021
·
IF 4.158
Dexin Chen,Yaoxin Li,Xueyang Zhao,Hongyuan He,Guodong Sun,Wei Li,Xiaojian Wang
In this study, different surface textures (Diamond, Cylinder, and Sin) have been designed and produced on Ti6Al4V alloys by selective laser melting technology. TiO 2 /Ag composite coatings were prepared on these textures by merging anodic oxidation and spray deposition methods. Tightly arranged TiO 2 nanotubes (TNTs) with tube diameters of about 100 nm are found on the top and lateral sides of the textures. On the bottom of the textures, dense nanopores were found with a pore diameter of about 50 nm. Compared with the untreated titanium alloy surface, both texture and TNTs structures would endow the titanium alloy surfaces with higher in vitro bioactivity. All three textures have an enhanced antibacterial property and a complete bactericidal effect was achieved by spray deposition of Ag nanoparticles (AgNPs) on all the surfaces. The MC3T3-E1 cells can adhere to the surfaces and proliferate normally, indicating good cytocompatibility of TiO 2 /Ag surfaces. Besides, the deposited AgNPs have been found to promote cell proliferation .
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Yu-Zhuang Gao,Tian-Xiao Chang,Yi-Xian Wu
Biomass sodium alginate (SA) and biomass-derived ether tetrahydrofuran (THF) have drawn considerable attention due to their sustainability and bio-renewability. In this paper, the novel acylated sodium alginate- g -polytetrahydrofuran graft copolymers (ASA- g -PTHF) with various PTHF branches ( M n,PTHF = 600–4800 g/mol, G N = 3–20 per 1000 SA monosaccharide) could be successfully synthesized by combination of THF living cationic ring-opening polymerization with grafting-onto method. The microphase separation of ASA- g -PTHF graft copolymer formed via self-assembly from two kinds of segments in backbone and side chains and was emphasized by thermal annealing, leading to a remarkable increase in water contact angle on polymer surface even by 38.4°. The hydrophilicity and crystallization behavior of ASA- g -PTHF can be decreased with increasing M n,PTHF and G N , which confine the crystallization of graft copolymer. ASA- g -PTHF microspheres (~1 μm) loaded with ibuprofen shows pH sensitivity and reach 100% of drug-release at pH = 7.4 within 30 h. ASA- g -PTHF exhibits resistance to bovine serum albumin adsorption and its composite with small-scaled silver nanoparticles (Ag-NPs, <2.6 wt%, 5–15 nm) generated in - situ behaves good antibacterial performance against E. coli and S. aureus . The ASA- g -PTHF would contribute to the better utilization of biomass and has promising for biomedical applications.
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Hongliang Tan,Gonge Tang,Chanjiao Ma,Qian Li
In this work, we developed a simple and sensitive method for the detection of cysteine (Cys) by employing terbium ion (Tb 3+ )-promoted G-qudraplex (G4/Tb) as a luminescent probe, which is based on Ag + -mediated conformational change of G4/Tb. Due to Ag + is able to compete with Tb 3+ to bind guanine at G4, the presence of Ag + can lead to the formation of G4/Tb–Ag + complex and disrupt the structure of G4/Tb. Meanwhile, the binding of Ag + with G4/Tb will also cause the alteration of the excited state of G4 and more efficient energy transfer from G4 to Tb 3+ , enhancing the luminescence of G4/Tb. However, upon the addition of Cys, Ag + will be released from G4/Tb–Ag + complex because of the high affinity of Cys to Ag + . This results in the re-formation of the conformation of G4/Tb and the decrease of the luminescence of G4/Tb. So, Ag + -enhanced luminescence of G4/Tb is associated with its conformational transformation. As a luminescent probe for Cys, G4/Tb not only shows excellent selectivity and sensitivity with a detection limit of 20 nM, but also possesses the features of simple preparation, easy reproducibility, and eliminating the interferences from background fluorescence. We envision that the presented strategy might provide new insight into the biosensing applications of lanthanide complex.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-9
·
IF 6.232
Wei Yu-ping,Zhang Yi-wen,Chen Jing-Shuai,Mao Chang-jie,Jin Bao-Kang
An ultrasensitive electrochemiluminescence biosensor was established based on the Zn-MOF/GO nanocomposite. Ag(I)-embedded DNA complexes were used as a signal amplification reagent. In this work, 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin (TCPP) and Zn 2+ were integrated into a porphyrin paddlewheel framework (Zn-MOF) by a hydrothermal method. The synthesized Zn-MOF material has electrochemiluminescence property, and the luminescence intensity is improved after being composited with graphene oxide (GO). Based on the composite material, we constructed an ultrasensitive ECL biosensor for the p53 antibody detection. The composite material acted as an admirable substrate and then loaded plenty of p53 antigens to recognize the target (p53 antibody) accurately. Because of the bridging effect of streptavidin and biotin-conjugated goat anti-rabbit IgG (bio-ab2), the rich-C DNA with positive correlation with the target was modified on the electrode and then captured the co-reactant accelerator Ag + to amplify the signal. Therefore, the ECL biosensor response increases with increasing p53 antibody concentration. In the range 0.1 fg/mL–0.01 ng/mL, the response signal of the biosensor has a good linear relationship with the p53 antibody concentration. The detection limit is 0.03 fg/mL (S/ N = 3). Impressively, the biosensor not only featured high sensitivity, good stability, and excellent specificity for the detection of p53 antibody, but also provides a new way for early detection of cancer.
Schematic representation of the electrochemiluminescence sensor based on a Zn-MOF/GO nanocomposite, which can be applied to the determination of p53 antibody.
✓ 已验证
DOI
PubMed
高影响因子
FUEL
·
2022
·
IF 8.035
Ying Zhang,Chen Huang,Fange Kong,Yufei Wang,Quan Shi,Linzhou Zhang
Molecular characterization of olefins in the complex hydrocarbon mixture is of vital importance for the various hydrocarbon upgrading processes. According to the strong interaction of Ag + with unsaturated hydrocarbon, Ag + complexation was adopted in this study as a tool to achieve selective olefin ionization in electrospray ionization (ESI). High-resolution mass spectrometry (HR MS) was used to characterize ionized olefin molecular composition. Various model compounds, including paraffins, naphthenes, olefins, and aromatics, were used to verify the ionization selectivity. The observed olefin carbon number distribution from Ag + complexation ESI HR MS was in agreement with those from gas chromatography-mass spectrometry equipped with the electron impact ion source (GC-EI-MS). The proposed method can be conveniently used for direct molecular characterization of olefins in mixtures without time-consuming olefin pre-separation or chemical derivatization. Olefins in coker diesel, coal tar liquid, and Fischer-Tropsch wax were successfully analyzed by the proposed method.
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2012
·Vol. 22
·期 45
·23981-23988
·
IF 5.968
Wan-Fu Ma,Lu-Lu Li,Ying Zhang,Qiao An,Li-Jun You,Ju-Mei Li,Yu-Ting Zhang,Shuai Xu,Meng Yu,Jia Guo,Hao-Jie Lu,Chang-Chun Wang
Selective enrichment of glycopeptides from complicated biological samples is essential for MS-based glycoproteomics, but still remains a great challenge. In this study, we report an unprecedented ligand-free strategy for the selective enrichment of glycopeptides by simply utilizing the multivalent interaction between glycopeptides and silver nanoparticles (Ag-NPs) coated magnetic nanoarchitectures. The composite microspheres were deliberately designed to be constructed with a high-magnetic-response magnetic colloid nanocrystal cluster (MCNC) core, a poly(methacrylic acid) (PMAA) interim layer and a Ag-NPs functional shell with high coverage. Taking advantage of the reversible interaction of glycans with Ag-NPs and the high magnetic susceptibility of the magnetite core, the MCNC@PMAA@Ag-NPs microspheres possess remarkable selectivity for glycopeptides even at a low molar ratio of glycopeptides/non-glycopeptides (1 : 100) with a very rapid enrichment speed (only 1 min needed) and a simple operation procedure using magnetic separation. Applying this approach, we identified 127 unique glycopeptides mapped to 51 different glycoproteins from only 1 μL rat serum samples. These results clearly demonstrated that the MCNC@PMAA@Ag-NPs have great potential for purifying and identifying the low-abundant glycopeptides in complex biological samples.
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Hengye Chen,Ou Hu,Haiyan Fu,Yao Fan,Lu Xu,Qingjun Meng,Lei Zhang,Wei Lan,Chunying Wu,Siyi Tang,Yuanbin She
A fluorescence paper-based sensor method by simply using thiomalic acid modified CdTe (TMA-capped CdTe) was proposed for visual detection of silver ions (Ag + ). Red-emission TMA-capped CdTe with a large amount of carboxyl groups on the surface could be combined with Ag + by electrostatic interaction, thus leading to the fluorescence quenching. It was found that the adding of amino acids contributed to fluorescence recovery by the leaving of Ag + on the surface of TMA-capped CdTe. Moreover, the more amino group, benzene and thiol group equipped in the structure of amino acid, the stronger fluorescence restoring of CdTe could be achieved. This fluorescence spectrometry method for Ag + showed low detection of limit with the value of 13.16 nmol/L, with no response to other heavy metals, which demonstrating its high sensitivity and high selectivity for Ag + . More importantly, this paper-based sensor method combined with chemometrics could accurately identify and quantify different concentrations of Ag + in different complex matrices. This fast, economical, accurate and effective approach exhibited a new idea for the on-site detection of Ag + , and was expected to be extended to the on-site detection of Ag + in biological and environmental fields.
✓ 已验证
DOI
高影响因子
POLYMER
·
2022
·
IF 4.432
Ruizhe Lin,Zhengqiao Yin,Yuanyuan Sun,Shengmiao Zhang
This work demonstrates an ex-situ swelling strategy for fabrication of the robust hierarchically porous polyHIPE. Specially, a cationic polyHIPE was prepared by copolymerization of 2-(methacryloyloxy) ethyltrimethylammonium chloride, styrene and divinylbenzene within a water-in-oil (w/o) high internal phase emulsion (HIPE). The hierarchically porous polyHIPE was achieved via simply swelling the polyHIPE in decane at 70 °C, and subsequent removal of the solvent at room temperature . There were large amounts of small pores found on the void wall surface. Compared with the hierarchically porous polyHIPE fabricated with decane as porogen (in-situ swelling, conventional route), the ex-situ swelling strategy endowed the materials semblable complex pore morphology but much less damage to its compression performance. The young's modulus of the hierarchically porous polyHIPE herein was 37% higher than that obtained by a conventional way. Afterwards, the hierarchically porous polyHIPE was utilized as a carrier for AgNPs (AgNPs@polyHIPE). The AgNPs@polyHIPEs exhibited eminent catalytic activity and recyclability for the reduction of the rhodamine B. Thereby, a facile approach for preparation of high strength and hierarchically porous polyHIPE was presented, and the resulted polyHIPE can be an ideal support for noble metal nanoparticle catalysts.
✓ 已验证
DOI
高影响因子
ACS SYMPOSIUM SERIES
·
2014
·
IF 0
Chun He,Yibing Gong,Shuzhen Li,Lingling Hu,Yichang Yang,Lianpeng Sun,Dong Shu
A visible-light-driven photo fuel cell (PFC) system, consisting of a Ag-BiOI/ITO photoanode and a Pt photocathode, was used to generate electricity by using wastewater organic compounds as electrolyte and fuel. The prepared BiOI and Ag-BiOI/ITO photoanodes were characterized by X-ray diffraction (XRD), scanning electronic microscope (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and UV-Vis diffuse reflectance spectroscopy (DRS) to examine the surface structure, chemical composition, and the properties of light absorption. The photoactivity of Ag-BiOI/ITO photoanodes was evaluated in the term of the electricity generation efficiency and degradation efficiency for recalcitrant organic pollutants. Compared with a BiOI/ITO photoanode, it was found that the efficiency of electricity generation using the Ag-BiOI/ITO photoanode was significantly higher in PFC system under visible light. Similarly, the photocatalytic degradation efficiency of wastewater organic pollutants using Ag-BiOI/ITO photoanode was significantly higher than that of BiOI/ITO. The enhancement in the electricity generation efficiency and degradation efficiency for wastewater organic pollutants is attributed to the action of these dopants Ag on BiOI surface, which play a key role by driving the electrons to the cathode via an external circuit.
✓ 已验证
DOI
Journal of Materials Chemistry B
·
2019
·Vol. 8
·期 4
·666-676
·
IF 5.047
Yan Zu,Liang Yan,Tao Wang,Dongqing Ma,Xinghua Dong,Zhen Du,Wenyan Yin
The non-invasive imaging of the gastrointestinal (GI) tract is highly desired for clinical research due to the various GI tract bacterial infection-induced diseases. To treat GI tract infections, various antibiotics have been used in the clinic. The growing problem of multidrug-resistant bacteria calls for effective antibiotic alternatives. Here, we construct a dual-functional Bi2S3@mSiO2@Ag nanocomposite for simultaneous enhanced X-ray computed tomography (CT) imaging and efficient antibacterial activity in the GI tract. The nanocomposite also has good stability, low cytotoxicity, and negligible hemolysis. Moreover, the investigation of the long-term toxicity and biodistribution of the Bi2S3@mSiO2@Ag nanocomposite after oral administration confirms its safety at the tested dosage. In particular, Ag nanoparticles (NPs) well dispersed on a silica substrate can reduce the antibacterial dosage and enhance the antibacterial activity of the Bi2S3@mSiO2@Ag nanocomposite. Furthermore, we have established bacterially infected enteritis animal models to confirm the antibacterial ability of the nanocomposite. This work opens up a new avenue for the design of a nanotheranostic agent that acts as both a contrast agent for the enhanced visualization of the GI tract and an antibacterial agent as an alternative to antibiotics.
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2012
·Vol. 4
·期 11
·3785-3791
·
IF 1.547
Essy K. Fodjo,Sara Riaz,Da-Wei Li,Lu-Lu Qu,Niamien P. Marius,Trokourey Albert,Yi-Tao Long
A Surface Enhanced Raman Scattering (SERS) substrate based on Ag/β-AgVO3 nanobelts deposited on copper foil (Cu@Ag/β-AgVO3) was used for the detection of carbamate pesticides: carbofuran, carbaryl, isoprocarb and propoxur. Cu@Ag/β-AgVO3 has shown an excellent SERS activity for carbamate pesticides compared to silver nanoparticles (AgNPs). Under optimized conditions, detection limits of 2.5 pM, 10 pM, 50 pM and 75 pM were obtained for carbaryl, carbofuran, isoprocarb and propoxur respectively, suggesting that Cu@Ag/β-AgVO3 is a good candidate for use as a SERS substrate for the trace level detection of these pesticides.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）