Acta Biomaterialia
·
2021
·
IF 8.947
Kesi Wang,Linping Jiang,Liyan Qiu
L-arginine (L-Arg) is an important nitric oxide (NO) donor, and its exploration in NO gas therapy has received widespread attention. Application of nano-platforms that can efficiently deliver L-Arg and induce its rapid conversion to NO becomes a predominant strategy to achieve promising therapeutic effects in tumor treatment. Herein, an enhanced nano-vesicular system of ternary synergistic treatment combining NO therapy, photodynamic therapy (PDT) along with mild photothermal therapy (MPTT) was developed for cancer therapy. We integrated photosensitizer PEGylated indocyanine green (mPEG-ICG) into polyphosphazene PEP nano-vesicles through co-assembly and simultaneously encapsulated NO donor L-Arg into the vesicle center chambers to form mPEG-ICG/L-Arg co-loaded system IA-PEP. The unique nanostructure of vesicle provided considerable loading capacity for mPEG-ICG and L-Arg with 15.9% and 17.95% loading content, respectively, and efficiently prevented mPEG-ICG and L-Arg from leaking. Significantly, the reactive oxygen species (ROS) was produced by IA-PEP under 808 nm laser irradiation to perform PDT against tumors, which concurrently reacted with L-Arg to release NO and arouse gas therapy effectively. Moreover, the mild heat produced by IA-PEP could exhibit cooperative anti-tumor effect with minimal damage. As a consequence, in vivo antitumor investigation on nude mice bearing xenograft MCF-7 tumors verified the potent anti-tumor efficacy of IA-PEP under 808 nm laser irradiation with complete tumor elimination. Taken together, the IA-PEP nano-vesicle system designed in this work may provide a promising treatment paradigm for synergistic cancer treatment. Statement of significance Nitric oxide (NO) gas therapy has drawn widespread attention due to its “green” treatment paradigm with negligible side effects. L-arginine (L-Arg) is an important NO donor. However, how to efficiently deliver L-Arg and induce NO generation remains a big challenge since L-Arg is a water-soluble small molecule . Herein, we developed a nano-vesicle system IA-PEP to integrate photosensitizer PEGylated indocyanine green and L-Arg with high loading content and to produce a ternary synergistic treatment combining NO therapy, photodynamic therapy (PDT) along with mild-temperature photothermal therapy (MPTT) under 808 nm laser irradiation. The in vivo investigation on nude mice bearing xenograft MCF-7 tumors verified its potent anti-tumor efficacy with complete tumor elimination.
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CHEMICAL ENGINEERING JOURNAL
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2021
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IF 13.273
Xin Zhou,Bowen Li,Mingming Guo,Wenchang Peng,Deping Wang,Qinglu Guo,Shuchao Wang,Dong Ming,Bin Zheng
Controlling medication according to the condition has become a universal treatment regimen for Parkinson's disease (PD) worldwide. However, the general oral administration method is prone to a variety of digestive system discomfort, easy to be broken down by digestive juices and intestinal microorganisms, resulting in low absorption efficiency, and a serious delay in the efficacy of the drug. Here, we developed a microneedle patch (MNP) that could release drugs with controlled temporal and spatial precision triggered by near-infrared (NIR) light for the treatment of PD. The microneedles (MNs) took Gelatin-methacryloy (GelMA) as the matrix, and therapeutic drugs (L-DOPA) were stored in the mesopore of the upconversion micron-rods (UCMRs), dispersing in the GelMA. The molecular motor, as a poreblocker and gate switch, isomerizes under the excitation of ultraviolet and visible light from UCMRs for controlling drug release. This kind of MNP penetrates the epidermis in a painless, non-invasive, and non-infectious way. Under the exposure of NIR light, the L-DOPA in the MNs will be released as needed, and then it seeps into the blood and brain for relieving the symptoms of PD. In the treatment of Parkinson's mice model, this regimen significantly restored motor function, and further mechanistic studies revealed that it reduced neuroinflammation and dopaminergic neuronal death in the substantia nigra. Besides, the released L-DOPA directly entered the blood, which reduces the side effects on the gastrointestinal tract and improves the utilization rate of the drug. These attractive superiorities indicated that the MNP with controlled drug delivery system may be candidate strategies for the treatment of PD and can be used in various related biomedical fields.
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Analytical Methods
·
2021
·Vol. 13
·期 32
·3561-3568
·
IF 2.896
Lihong Shi,Xiaorui Dong,Guomei Zhang,Yan Zhang,Caihong Zhang,Chuan Dong,Shaomin Shuang
N-doped blue-fluorescence carbon dots (N-CDs) were fabricated via a one-pot hydrothermal method using folic acid and p-phenylenediamine. The obtained N-CDs exhibited strong fluorescence (FL) with a considerable quantum yield (QY) of 21.8% and exceptional optical stability under different conditions. Upon introducing Cr(VI), blue FL of N-CDs was distinctly quenched. On subsequent addition of L-AA, the FL of N-CDs could be partially recovered. The fluorescence changes of N-CDs have been utilized to detect Cr(VI) and L-AA in aqueous solutions with linear ranges of 0.10–150 μM and 0.75–2.25 mM, respectively, as well as limit of detection values of 9.4 nM and 25 μM, respectively. Furthermore, as-obtained N-CDs can be extended to monitor the fluctuation of intracellular Cr(VI) and L-AA. More intriguingly, N-CDs can target lysosomes with a satisfactory Pearson correction coefficient of 0.87, which indicates a promising application prospect in the biomedical field.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
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IF 6.953
Linan Zhou,Ziteng Song,Shaojie Zhang,Yeling Li,Jing Xu,Yuanqiang Guo
Selenium nanoparticles (SeNPs), a potential cancer therapeutic agent, have attracted widespread attention owing to their high bioavailability and remarkable anticancer activity. Nevertheless, the poor water solubility and dispersibility of SeNPs seriously limit their applications. In the present study, we synthesized stable and individual spherical selenium nanoparticles (CL90-Tw-SeNP2) with an average diameter of approximately 79 nm using a polysaccharide extracted from Citrus limon (CL90) and Tween-80 as the decorator and stabilizers. The proportion of selenium in CL90-Tw-SeNP2 was 10.6%. CL90-Tw-SeNP2 possessed high stability and good dispersion in water for more than three months. The subsequent biological assay revealed that CL90-Tw-SeNP2 showed remarkable antitumor effects against HepG2 cells, with an IC 50 value of 49.13 μg/mL, by inducing cell apoptosis. Furthermore, an in vivo zebrafish assay to explore possible applications indicated that CL90-Tw-SeNP2 could inhibit the proliferation and migration of tumors and the zebrafish angiogenesis. These results indicated that CL90-Tw-SeNP2 could be a potential agent for cancer treatment, especially against human liver hepatoma cancer.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Xueying Song,Lei Wang,Ting Liu,Yongfeng Liu,Xiaoxia Wu,Liu Liu
Mandarin ( Citrus reticulata L.) essential oil (MEO) reportedly displays excellent antimicrobial properties . In this study, MEO was loaded into chitosan nanoparticles (CSNPs). The characteristics, antibacterial properties and benefit in pork preservation of MEO-CSNPs were evaluated. The MEO-CSNPs displayed an excellent encapsulation efficiency (EE) (67.32%–82.35%), the particle size values of 131.3 nm–161.9 nm, and the absolute zeta potential values above 30 mV. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, and thermogravimetric analysis (TGA) revealed that the MEO was incorporated into CSNPs without requiring a chemical reaction, the antibacterial activity of the MEO remained. Furthermore, the damage of MEO-chitosan nanoemulsions (MEO-CSs) to the cell membranes of Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) was confirmed by the change of bacterial cell morphology. The anti-biofilm assays verified that the MEO-CSs substantially inhibited biofilm formation and destroyed the mature biofilms. MEO-CSs were also applied to pork, proving a great potential for pork preservation. This study provides a potential approach for developing and utilizing MEO-CSs as natural antimicrobial agents in the food industry.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2021
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IF 6.291
Xueying Zong,Juanjuan Zhang,Jinwei Zhu,Linyu Zhang,Lijuan Jiang,Ying Yin,Hongyan Guo
Microplastics are widespread in freshwater environments, their biological effects and combined effects of other pollutants have attracted extensive attention. In this study, we investigated the adsorption properties of heavy metals onto polystyrene (PS) microplastics as well as the bioavailability and toxicity of microplastics and heavy metals by hydroponic wheat seedlings experiment. Results showed that PS microplastics (0.5 µm, 100 mg/L) had no significant effect on wheat seedlings growth, photosynthesis, and reactive oxygen species (ROS) content. However, PS microplastics could adsorb copper and cadmium, with a predominantly chemisorption. The accumulation of copper and cadmium in wheat seedlings reduced in the presence of PS microplastics, which meant the toxic effect by heavy metals might be mitigated. Compared with single heavy metals treatments, the combination of PS microplastics and heavy metals increased chlorophyll content, enhanced photosynthesis and reduced the accumulation of ROS. These findings suggest that PS microplastics (0.5 µm, 100 mg/L) have a mitigating effect on the bioavailability and toxicity of copper and cadmium.
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LANGMUIR
·
2021
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IF 3.882
Lingqi Zhong,Haizheng Tao,Xiao Gong
In this work, superhydrophobic poly(l-lactic acid) (PLLA) hierarchical membranes exhibiting excellent oil-removal performance, which is of great importance in curbing the oil-pollution environment, were fabricated by a simple solvent-evaporation-induced precipitation method. PLLA membranes with hierarchical micro/nanostructures (fish scales, fibrous sheets, and petal-like morphology) can be conveniently prepared by adjusting the preparation parameters including PLLA concentration, precipitation temperature, type of solvent and nonsolvent, and the addition of nano-SiO2. The results show that the water contact angle of the fish-scale-structured PLLA membrane was 138.6°, revealing that water repellency was significantly improved compared to that of the solvent-casting PLLA membrane (∼72.8°). Moreover, the PLLA/SiO2 nanocomposite membrane with a dense hierarchical micro/nanostructure had a water contact angle greater than 167.1°, which has great potential in oil–water separation.
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JOURNAL OF BIOLOGICAL CHEMISTRY
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2021
·Vol. 297
·期 1
·
IF 5.157
Bochuan Yuan,Ziwei Wu,Wei Ji,Dong Liu,Xiang Guo,Donghui Yang,Aili Fan,Hongli Jia,Ming Ma,Wenhan Lin
The fungal cyclohexadepsipeptides destruxins (DTXs), isaridins (ISDs), and isariins (ISRs) are nonribosomal peptides whose structures include a 19-membered ring composed of five amino acid residues and one α- or β-hydroxy acid residue. These cyclohexadepsipeptides contain unusual nonproteinogenic amino acid–building blocks and possess a range of antiviral, antibacterial, and other activities. The biosynthetic gene clusters for ISDs and ISRs have not been identified, and the biosynthesis of the nonproteinogenic (3S)-methyl-l-proline residue, which is found in DTXs, ISDs, and many other natural products, lacks full characterization. In an ongoing effort to identify compounds that can inhibit the Zika virus (ZIKV), we examined the extract of marine-derived fungus Beauveria felina SX-6-22 and discovered 30 DTXs, ISDs, and ISRs (1–30) including seven new compounds (1–7). The anti-ZIKV assays showed that 9–12 and 16–18 possess inhibitory activities against ZIKV RNA replication and NS5 (nonstructural protein 5) production in ZIKV-infected A549 cells. We sequenced the genome of B. felina SX-6-22 and identified three biosynthetic gene clusters detx, isd and isr, which are responsible for the biosynthesis of DTXs, ISDs, and ISRs, respectively. Comparative analyses of the three gene clusters clarified the biosynthetic relationships among these cyclohexadepsipeptides. Finally, we characterized the entire biosynthesis of nonproteinogenic building block (3S)-methyl-l-proline. The Δ1-pyrroline-5-carboxylate reductases (P5CRs), also used in the biosynthesis of l-proline, were demonstrated to catalyze the final reduction step in (3S)-methyl-l-proline formation, suggesting potential cross talk between primary and secondary metabolisms. These results provide opportunities for biosynthetic pathway engineering to generate new anti-ZIKV cyclohexadepsipeptides.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Xiuheng Xue,Haiyong He,Cunjun Liu,Li Wang,Lu Wang,Yueji Wang,Luping Wang,Chen Yang,Juhua Wang,Ruyan Hou
Diacylglycerol (DAG) is commonly used as fat substitute in food manufacture due to its functional properties, but DAG has poor emulsification and oxidation stability, which limits its wide application in food industry. In this work, fluorescence quenching data and thermodynamic parameters were analyzed to investigate the interaction mechanism between l -theanine (L-Th) and β-lactoglobulin (β-LG). DAG emulsion was prepared by using β-lactoglobulin-theanine (β-LG-Th) as surface stabilizer, and its emulsification and oxidation stability were evaluated. The results showed that the hydrophobic interaction played an important role on the conjugate of β-LG and L-Th due to the negative values for ΔG, positive values for ΔH and ΔS at pH 4.0, pH 6.0 and pH 8.0. The DAG has been better embedded by using β-LG-Th as surface stabilizer, and the droplet size was about 0.2 µm to 1.5 µm when the pH was 6.0, the ratio of L-Th to β-LG was 1:1. β-LG-Th as surface stabilizer for DAG can increase the ζ-potential and emulsion index, make the emulsion droplet size distribution more uniform. The l -theanine was better to be used to improve the emulsification stability and antioxidant capacity of DAG by binding β-LG as surface stabilizer.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jing Wen,Lijun Zhou,Ding Jiang,Xueling Shan,Wenchang Wang,Hiroshi Shiigi,Zhidong Chen
Herein, a sensitive and selective electrochemiluminescence (ECL) aptasensor was designed using [email protected] as accelerator towards the perylene derivative (PTC-Cys)/peroxydisulfate (S 2 O 8 2− ) system for kanamycin (KAN) assay. Firstly, the PTC-Cys was prepared by covalently binding l -cysteine to 3,4,9,10-perylenete-tracarboxylic acid, which was acted as the luminophore. Then [email protected] play the part of effective catalyst to accelerate the electrochemical reduction process of S 2 O 8 2− to produce more sulfate radical anions (SO 4 •- ), thus the ECL signal of the compound was noticeably raised by 2.4 times in comparison with that in which only luminophore and S 2 O 8 2− are present, achieving signal amplification of the ECL system. In the presence of KAN, aptamer was pulled down from the sensing interface, achieving a considerable enhancement of ECL intensity in S 2 O 8 2− solution. Upon the optimal condition, our proposed strategy can quantify the concentration of KAN from 1.0 × 10 −13 to 1.0 × 10 −8 M with low limit of detection of 4.2 × 10 −14 M (S/N = 3).Besides, our proposed ECL aptasensor exhibited excellent sensitivity, stability and specificity, and could be successfully applied to detect KAN in practical samples, which proved its potential to detect other antibiotics in food security.
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LUMINESCENCE
·
2021
·Vol. 36
·期 6
·1513-1524
·
IF 2.464
Huan Zhang,Zhenzhen Li,Huaying Huang,Si Ouyang,Yiqing Deng,Qiang Zhao
Near-infrared (NIR) core–shell CdSeTe/CdS quantum dots (QDs) modified with l -cysteine were synthesized in aqueous solution. The QDs had a special NIR-emitting spectrum, high fluorescence stability and low cytotoxicity. In addition, they exhibited an obvious fluorescence quenching when Cu 2+ was present. An NIR nanosensor was prepared for rapidly, sensitively, and selectively determining Cu 2+ in solution quantitatively and monitoring the changes in Cu 2+ in cells with fluorescence imaging in a semiquantitative way. The linear relationship between the relative fluorescence intensity (F 0 /F) and the concentration of Cu 2+ from 5.12 × 10 −8 M to 2.56 × 10 −5 M in solution was observed using an NIR fluorescence spectrophotometer with R 2 equal to 0.9958. Moreover, in the experiment with the fluorescence microscope, F 0 /F versus the concentration of Cu 2+ from 5.00 × 10 −8 M to 7.68 × 10 −6 M also showed a good linear relationship with R 2 equal to 0.9817. Practical water sample ion detecting experiments had good accuracy and recovery rates. Cell experiments showed that the NIR imaging intensity of cells was inversely proportional to the concentration of copper ions, therefore NIR QDs have great potential for detection of metal ions in solution and in cells.
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Natural Product Communications
·
2020
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IF 0.468
YanHua Tian,HuiZhi Du,Li Wang,ShiFei Li,Lu Zhang,LiWei Zhang
Diospyros lotus L. leaves are used as a functional tea and folk medicine in several Asian countries. This study aimed to evaluate the effects of phenolic extracts of the leaves on scavenging nitrite and inhibiting N-nitrosamines (NAs) formation and to determine the active ingredients responsible for these effects. Of the 7 fractions (Fr1-Fr7) prepared from the extract of D. lotus leaves, Fr5 contained the highest phenolic content and exhibited the strongest activity. Five active ingredients of Fr5 were discovered, and 4 of them were identified as myricitrin (Mytr), myricetin (Myt), myricetin-3-O-β-d-glucoside (Myt-Glc), and myricetin-3-O-β-d-galactoside (Myt-Gal). The content of Mytr was much higher than those of the other 3 ingredients, both in Fr5 and extracts of D. lotus leaves. Finally, Mytr and Myt were proved to have stronger activities by the 1,1-diphenyl-2-trinitrophenyl hydrazine scavenging, nitrite scavenging, and inhibition of NAs formation assays. These results indicated that Mytr was the main active ingredient of D. lotus leaves. Myt, Mytr, and Fr5 from the leaves could be used as natural agents for antioxidant, nitrite scavenging, and inhibition of NAs formation in food and the human body.
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危险品化学品经营许可证（带存储）