ACS Applied Nano Materials
·
2024
·
IF 5.3
Chu-shu Zhu,Siyue Xiong,Chengxuan Wang,Peitao Dong,Xuezhong Wu
Accurate and convenient diagnosis of the influenza virus is of critical importance to prevent the spread of flu infections and guide therapy and treatment. This work reports the fabrication of a sensitive and quantitative surface-enhanced Raman scattering (SERS)-based lateral flow (LF) strip combined with enzymatic recombinase amplification (ERA) for the highly effective detection of the influenza A virus. Gold-shell silica-core nanoraspberries (NRbs) were used in this SERS-LF strip as a SERS substrate. Practical and convenient detection was accomplished by naked-eye visualization, and highly sensitive quantitative detection was accomplished by monitoring the intensity of the distinct Raman peaks associated with the SERS tags. Under optimal conditions, the sensitivity of this ERA-SERS-LF strip for the DNA standard of the IAV matrix gene is 105 copies/mL by naked-eye visualization, and the limit of detection (LOD) was estimated to be 2.63 × 103 copies/mL by SERS detection, lowered by nearly 40 times compared with visual results due to the signal enhancement of the SERS tags. Besides, quantitative detection for the DNA standard of the IAV matrix gene was obtained with a wide linear range (2.63 × 103 copies/mL to 109 copies/mL). Pseudoviruses of IAV and other respiratory viruses could be successfully identified by using the fabricated ERA-SERS-LF strip. Overall, the findings of this study demonstrate the prospects of the use of the ERA-SERS-LF strip as an effective tool for point-of-care testing (POCT) detection of viruses by virtue of its portability, good sensitivity, quantitative detection ability, and outstanding specificity.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-10
·
IF 5.3
Peng Sasa,Zhang Zhilong,Xin Mingwei,Liu Dongli
An innovative method is introduced based on the combination of label-free surface-enhanced Raman scattering with advanced multivariate analysis. This technique allows both quantitative and qualitative assessment of Salmonella typhimurium and Escherichia coli on eggshells. Using silver nanocubes embedded in polydimethylsiloxane, we consistently achieved Raman spectra of bacteria. The stability of the Ag NCs@PDMS substrate is confirmed using rhodamine 6G over 30 days under standard conditions. Principal component analysis (PCA) effectively distinguishes between S. typhimurium and E. coli spectra. Partial least squares regression (PLS) models were developed for quantitative determination of bacteria on egg surfaces, yielding accurate results with minimal error. The S. typhimurium model achieves R c 2 = 0.9563 and RMSEC = 0.601 in calibration, and R v 2 = 0.9113 and RMSEV = 0.907 in validation. Similarly, the E. coli model achieves R c 2 = 0.9877 and RMSEC = 0.322 in calibration, and R v 2 = 0.9606 and RMSEV = 0.579 in validation. Recoveries validate PLS predictions by inoculating egg surfaces with varying bacterial amounts. Our study demonstrates the feasibility of SERS-PLS for quantitative determination of S. typhimurium and E. coli on eggshells, promising enhanced food safety protocols. Graphical Abstract
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Zhilei Zhao,Mengyu Ren,Wenqi Zhang,Zikuo Chen,Lu Zhang,Xinquan Qu,Junling Shi,Weiwei Xia,Xiaoguang Xu,Ying Yang
In view of the serious harm and widespread pollution caused by deoxynivalenol (DON), it is crucial to quantify its presence in the food safety assessment process. Here, we designed a core-shell-satellite nano assembly structure as a probe, composed of an anisotropic AuNR@Ag core, an ultra-thin SiO 2 layer and a high surface coverage AuNPs satellites, which showed outstanding SERS activity and high stability. Anti-DON antibodies were modified on the surface of the prepared core-shell-satellite nano-assembly structure as SERS immunoprobe for DON specific detection using SERS-based lateral flow immunoassay (LFIA) with the advantages of simplicity, rapidity, and high sensitivity. Under optimal conditions, the detection limit for detecting DON using the SERS-LFIA method was 0.053 fg/mL, and the linear detection ranged from 0.1 fg/mL to 1 μg/mL. The SERS-LFIA strips based on AuNR@Ag@SiO 2 -AuNP nanostructures demonstrated the great potential for quick quantitative DON detection, providing a reference for trace detection of highly toxin mycotoxins.
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Food Bioscience
·
2025
·
IF 5.9
Junkui Li,Chen Li,Wang Guo,Yiqing Guo,Xiaobo Zou,Zongbao Sun
Gutter oil, which poses a serious threat to human health and has generated widespread attention in recent years, is misused as cooking oil, creating a major food safety concern. Capsaicin has been identified as a promising indicator for the detection of gutter oil. Hence, in this study, we introduce a novel hybrid approach for capsaicin detection that combines magnetic molecular imprinting (MMIP) with surface-enhanced Raman scattering (SERS) technology to rapidly and accurately identify gutter oil. A magnetic molecularly imprinted polymer is developed with the incorporation of purified halloysite nanotubes, which provide a large specific surface area. The resulting product, magnetic halloysite molecularly imprinted polymers (MHNTs@MIPs), serves as a specific isolation material for the enrichment of capsaicin from complex matrices. The Ag NPs@Si (silver nanoparticles deposited on a silicon wafer surface) and Au NPs (gold nanoparticles), known for their excellent signal enhancement capabilities, work synergistically as the SERS substrate. Substantially enhanced SERS peaks are observed at 1173 cm −1 , indicating the potential of this method for capsaicin detection and gutter oil identification. A strong log-linear relationship between SERS intensity and the logarithm of capsaicin concentration is established across a wide range of 0.05–100 μM, with a detection limit of 67.7 nM. Furthermore, for both simulated and real gutter oil samples, capsaicin recovery rates of 95.5%–110.3% and 96.3%–104.9% were achieved using the SERS sensor, demonstrating good consistency with certified high-performance liquid chromatography (HPLC) results. Therefore, this SERS sensor can be served as efficient method for detecting capsaicin and monitoring oil quality.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaojun Wen,Yiting Liu,Weilong Zhang,Lijun You,Ning Cai,Jumei Li
In this research, we fabricated an innovative NiO/CN/Ag composite through the straightforward electrostatic assembly of Ag nanoparticles and g-C 3 N 4 sheets onto NiO nanoflowers. This composite enables both surface enhanced Raman spectroscopy (SERS) detection and photo-degradation of pesticides. The results reveal NiO/CN/Ag can quantitatively detect thiram (TRM) and diquat dibromide (DQDB) in water, with a limit of detection (LOD) of 10 −9 M, and also exhibit outstanding photo-degradation efficiency, exceeding 95 % for TRM and DQDB within 90 min under simulated sunlight. Significantly, after the organic reagent (citric acid) on NiO/CN/Ag was completely removed through simulated solar irradiation, the signal-to-noise ratios of SERS spectra on NiO/CN/Ag were enhanced, achieving LODs of 10 −8 M for TRM and DQDB in different fruit juices and dried flower teas. Additionally, the composite demonstrate impressive recyclability, maintaining robust SERS signals and high degradation rates even after five cycles of “detection-degradation” processes.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Ailing Su,Yuan Liu,Weihan Sun,Chongyang Liang,Weiqing Xu,Alison Rodger,James Piper,Yuling Wang,Shuping Xu
Specifically identifying one-base mismatched gene sequences is of significance for early diagnosis, prognosis, treatment, and evaluation. However, traditional polymerase chain reaction and next-generation sequencing methods require relatively long, carefully implemented analytical procedures. Here, we report a nanoconjugate that can synchronously discriminate two types of KRAS gene mutation due to the dual-recognition mechanism of the CRISPR/dCas system with the surface-enhanced Raman scattering (SERS) response output. This method applied two sets of CRISPR/dCas9 units to collect all KRAS genes via a magnetic force and the specific mutant sequence labeled through SERS nanotags, forming a dual-clamping architecture. This sensing conjugate was sufficient to quantify the amount of the two different mutations simultaneously with a low cross-reactivity. Consequently, we deem that this sensing assay satisfies the great characteristics of a genetic analytical tool with high specificity, accuracy, and simplicity, highlighting its potential as an alternative for screening multigene events and relevant genetic biomarkers.
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Microsystems & Nanoengineering
·
2024
·Vol. 10
·期 1
·1-13
·
IF 7.3
Han Donglai,Guo Boyang,Li Yanru,Feng Wei,Liu Keyan,Wu Tianna,Wan Yuchun,Wang Lili,Gao Ming,Liu Yang,Yang Lili,Wei Maobin,Yang Shuo
Facile and efficient photocatalysts using sunlight, as well as fast and sensitive surface-enhanced Raman spectroscopy (SERS) substrates, are urgently needed for practical degradation of tetracycline (TC). To meet these requirements, a new paradigm for PI/TiO 2 /Ag organic‒inorganic ternary flexible microfibers based on semiconducting titanium dioxide (TiO 2 ), the noble metal silver (Ag) and the conjugated polymer polyimide (PI) was developed by engineering a simple method. Under sunlight, the photocatalytic characteristics of the PI/TiO 2 /Ag flexible microfibers containing varying amounts of Ag quantum dots (QDs) were evaluated with photocatalytic degradation of TC in aqueous solution. The results demonstrated that the amount of Ag affected the photocatalytic activity. Among the tested samples, PI/TiO 2 /Ag-0.07 (93.1%) exhibited a higher photocatalytic degradation rate than PI/TiO 2 (25.7%), PI/TiO 2 /Ag-0.05 (77.7%), and PI/TiO 2 /Ag-0.09 (63.3%). This observation and evaluation conducted in the present work strongly indicated a charge transfer mechanism. Moreover, the PI/TiO 2 /Ag-0.07 flexible microfibers exhibited highly sensitive SERS detection, as demonstrated by the observation of the Raman peaks for TC even at an extremely low concentration of 10 –10 moles per liter. The excellent photocatalytic performance and SERS detection capability of the PI/TiO 2 /Ag flexible microfibers arose from the Schottky barrier formed between Ag and TiO 2 and also from the outstanding plasmonic resonance and visible light absorptivity of Ag, along with immobilization by the PI. The successful synthesis of PI/TiO 2 /Ag flexible microfibers holds significant promise for sensitive detection and efficient photocatalytic degradation of antibiotics.
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Applied Sciences-Basel
·
2024
·Vol. 14
·期 5
·2118
·
IF 2.5
Mengping Huang,Shuai Ma,Jinrong He,Wei Xue,Xueyan Hou,Yuqi Zhang,Xiaofeng Liu,Heping Bai,Ran Li
Amino acids found in minor coarse cereals are essential for human growth and development and play a crucial role in efficient and rapid quantitative detection. Surface-enhanced Raman spectroscopy (SERS) enables nondestructive, efficient, and rapid sample detection. Traditional SERS detection efficiency is constrained by the use of a single target. In this study, three different amino acids (cysteine, valine, and tryptophan) were detected simultaneously using a ZIF-8@AuNPs composite substrate. The linear range of detection was 10−3to 10−1M, with limits of detection (LODs) of 2.40 × 10−4M, 2.24 × 10−4M, and 1.55 × 10−4M, respectively. Same linear ranges and LODs were achieved with a one-dimensional convolutional neural network method. Furthermore, this substrate enabled the effective detection of amino acids in millet and efficient detection of cysteine in health products. This study presents a novel method for simultaneous detection of multiple analytes.
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Journal of Materials Chemistry A
·
2024
·
IF 10.7
Di-Ye Wei,Ge Zhang,Hong-Jia Wang,Qing-Na Zheng,Jing-Hua Tian,Hua Zhang,Jian-Feng Li
Revealing and understanding water activation and reaction at metal-oxide interfaces is of significant importance. However, it remains a great challenge due to the weak signal of surface-active species and the difficulty of in-situ detection methods. Herein, the water-gas shift reaction mechanism at the Pd-CeO2 interfaces has been systematically studied by using the “borrowing” surface-enhanced Raman spectroscopy (SERS) strategy through the fabrication of Au@CeO2-Pd core-shell satellite structures. Through the combination of in-situ SERS and online mass spectrometry, real-time monitoring of surface intermediate species and reaction products is achieved simultaneously. It is found that CO adsorbed on Pd can either react with the oxygen species formed via water dissociation (the associative mechanism) or the lattice oxygen in CeO2 (the redox mechanism), while the former has a higher activity. This work provides an effective way for the in-situ study of interfacial catalysis and explains the important role of the Pd-CeO2 interfaces in the water-gas shift reaction at a molecular level.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Tingting Hu,Xiaoyu Han,Haimei Zeng,Jihong Zhang,Jun Xie,Jianjun Han
Photocatalytic compound decomposition can be an effective protocol for the prominent and urgent solving of the environmental pollution and energy shortage problems. In-situ monitoring of the substance concentrations change during the process is of importance for understanding the decomposition process and final effects. In present research, a surface enhanced Raman spectroscopy (SERS) fiber probe based photocatalyst decomposition monitoring method is proposed. Ag nanoparticles and Ag 3 PO 4 microcube composites were synthesized by ion exchange and subsequent ion reduction method, and then they were coated on silica fiber end-tip for probe fabrication. The microstructures, elements distribution, and morphologies analysis confirm the formation of dense Ag nanoparticles with 30 nm diameter on 1 μm edge length Ag 3 PO 4 cubes. The localized surface plasmon resonance (LSPR) induced absorption peak of Ag nanoparticles is found at 318 nm, along with the band-gap absorption of Ag 3 PO 4 cubes. The Ag nanoparticles and Ag 3 PO 4 composites are homogeneously distributed on the fiber end-tip surface. The fiber probes show the lowest detection limit of 10 −14 M using crystal violet analyte, and as high as 5.3 × 10 11 calculated enhancement factor, due to the strong LSPR induced electrical field from the densely distributed Ag nanoparticles on Ag 3 PO 4 microcubes surface. Furthermore, the linear relationship between analyte concentrations and SERS intensities indicates a potential for quantitative analysis. The analytes crystal violet and 4-aminothiophenol can be photodegraded under UV and visible light irradiation by Ag 3 PO 4 . The Raman spectra of crystal violet and 4-aminothiophenol intensities decrease with longer irradiation durations, indicating the decomposition of organic compounds. The fiber probes also present repeatable using within several cycles. The research provides a potential method for photocatalyst decomposition monitoring.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Qian Zhang,Meiqi Bao,Ling Zhang,Cuicui Fu,Xiaozhe Zhang,Huiting Wang,Yaxian Chen,Chunfang Zhao,Xiaoyan Ma,Jiarui Xia
The rational design of colloidal interfaces with spatiotemporal control over electromagnetic coupling is essential for enhancing the sensitivity of surface-enhanced Raman scattering (SERS) sensitivity. In this study, we propose a tyramine-triggered Ag Au nanoassembly strategy, driven by enzymatic polymerization-mediated interfacial engineering. The horseradish peroxidase/H₂O₂ system induces oxidative crosslinking of tyramine-functionalized Ag nanoparticles (Ag NPs), facilitating precise assembly with antigen-immobilized Au substrates to generate plasmonic architectures. Finite-difference time-domain simulations show that the Au Ag bilayer configuration exhibits enhanced electromagnetic field intensity compared to monolayer Au NPs or Ag NPs. This enhancement is attributed to synergistic interfacial coupling at the Au Ag nanogaps and intra-layer plasmon resonance. The dynamic nanoassembly achieved ultrasensitive aflatoxin B₁ detection with a limit of detection of 0.11 pg/mL, high interference resistance, and a wide dynamic range (0.001–100 ng/mL). The quantitative detection of AFB₁ in real samples demonstrates excellent accuracy (95.7–109.7 %) and precision (RSD < 7.5 %). By integrating enzymatic colloidal control with interfacial electromagnetic engineering, this work establishes a new paradigm for field-deployable mycotoxin monitoring in complex food systems.
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Nanomaterials
·
2024
·Vol. 14
·期 12
·1046
·
IF 4.4
Xiangyang Li,Hean Qian,Jin Tao,Mingshuo Cao,Meng Wang,Wenlei Zhai
Extensive use of pesticides in agricultural production has been causing serious health threats to humans and animals. Among them, phorate is a highly toxic organophosphorus insecticide that has been widely used in planting. Due to its harmful effects on human and animal health, it has been restricted for use in many countries. Analytical methods for the rapid and sensitive detection of phorate residues in agricultural products are urgently needed. In this study, a new method was developed by combining surface-enhanced Raman spectroscopy (SERS) and immunochromatography assay (ICA). Hybrid magnetic Fe3O4@Au@DTNB-Ab nanoprobes were prepared by modifying and growing Au nanoseeds on an Fe3O4core. SERS activity of the nanoprobe was optimized by adjusting the concentration of the Au precursor. A rapid and sensitive assay was established by replacing the traditional colloidal gold-based ICA with hybrid SERS nanoprobes for SERS-ICA. After optimizing parameters including coating antibody concentrations and the composition and pH of the buffer solution, the limit of detection (LOD) for phorate could reach 1 ng/mL, with a linear range of 5~100 ng/mL. This LOD is remarkably lower than the maximum residue limit in vegetables and fruits set by the Chinese government. The feasibility of this method was further examined by conducting a spiking test with celery as the real sample. The result demonstrated that this method could serve as a promising platform for rapid and sensitive detection of phorate in agricultural products.
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