JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
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2020
·
IF 1.624
Kun Wu,Dan Zhao,Huifei Cui
The wound dressing can temporarily replace the skin and play a protective role in the process of wound healing, preventing wound infection and inflammation, and providing a favorable environment for wound healing. In this study, a mixture of collagen and chitosan was lyophilized to be the host material of the sponge. This sponge was soaked into 1-ethyl-(dimethylaminopropyl) carbodiimide/N-hydroxy sulfosuccinimide cross-linking solution containing heparin and experienced secondary lyophilization to prepare the heparinized sponge (CT-CL/Hp). The surface morphology and structural characterization of the sponge was characterized by scanning electron microscope and Fourier transform infrared spectrometer, respectively. Relatively favorable water absorption capability were observed by measuring the physical properties. Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro. The sustained-release property of heparin from the sponges was measured using Alcian Blue assay. Experiments in vitro and in vivo showed that the sponges had satisfactory biocompatibility and lower sensitization. Moreover, the effect of sponge on early stages of wound healing was evaluated by guinea pigs wound healing models. Analysis of wound healing rates and histological examination showed satisfactory results. CT-CL/Hp enhanced expression of growth factors, particularly VEGF and EGF at day 7. These results demonstrated that CT-CL/Hp–treated sponges benefit to wound skin healing and regeneration.
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SURFACE & COATINGS TECHNOLOGY
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2021
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IF 4.158
Lixuan Ren,Yanzhen Zhao,Liu Yang,Wenling Cao,Huifang Wang,Xiaojie Lian,Xianghua Gao,Baolong Niu,Wenfeng Li
The bacterial infection is one of the main reasons for the failure of implant surgery. In this paper, catechol functionalized chitosan (CSCT) was used as a reducing agent and stabilizer to synthesize CSCT-Ag NPs. The composite coating of titanium surface was then electrodeposited by a CSCT layer initially, followed by spin-coating of a CSCT/gelatin (G)/CSCT-Ag NPs layer. The results of XRD , UV–Vis, and TEM showed that the silver nanoparticles were successfully synthesized with uniform distribution, and the particle size was 4–22 nm. The cytotoxicity test results of CSCT-Ag NPs on A549 and EA.Hy926 cells showed when the concentration of CSCT-Ag NPs was less than 25 μg/mL, the survival rate of the two kinds of cells was greater than 80%. First electrodeposition and then spin-coating is feasible through the coating adhesion test and the adhesion level up to 5B. Compared with the pure Ti substrate, the corrosion resistance and hydrophilicity of the composite coating surface are significantly improved. The antibacterial rate of the composite coating against E. coli and S. aureus can reach 99% and 96%. In conclusion, CSCT-Ag NPs and the method of preparing coatings by electrodeposition and then spin-coating have good clinical application potential.
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CARBOHYDRATE POLYMERS
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2017
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IF 4.811
Liyun Rui,Minhao Xie,Bing Hu,Li Zhou,Danyang Yin,Xiaoxiong Zeng
Gallic acid-grafted-chitosan (GA- g -CS) was synthesized in the presence of 1-ethyl-3-(3′-dimethylaminopropyl) carbodiimide and hydroxybenzotriazole under room temperature. To develop a kind of newly functional film, CS, gelatin, glycerol and Tween 20 were utilized to prepare CS-based composite films with incorporated GA (GA-CS) or conjugated GA (GA- g -CS), and the films were characterized in aspects of physical and functional properties, as well as microstructures. With the increasing of GA concentration, there was a reduction in transmittance, elongation at break and water vapor permeability (WVP) for GA-CS film. For GA-CS films, the higher tensile strength and lower WVP could be obtained when GA was evenly distributed across the films. Different from the GA-CS films, water solubility, swelling ratio, water vapor permeability and scavenging activities on 2,2-Diphenyl-1-picrylhydrazyl/2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) radicals were enhanced for GA- g -CS films, which could be ascribed to the rough surface morphology. The GA- g -CS films exhibited enhanced water vapor permeability when the substitution degree of GA increased. Also, the antimicrobial activities of GA- g -CS films were superior to those of GA-CS films. Our present work developed a way to prepare GA- g -CS films that owned many desirable properties and could be explored as promising biomaterials in food packaging.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Linyu Nian,Yao Xie,Xiaoyang Sun,Mengjun Wang,Chongjiang Cao
The postharvest physiological and metabolic activities caused fruits and vegetables (F&V) quality deterioration. Therefore, developing an efficient preservation strategy is a promising approach to relieve this issue. In this study, a modified metal-organic framework (MOF; i.e., [email protected] ) was encapsulated into a blended matrix of chitosan quaternary ammonium salt (CQAS)/gelatin to fabricate a multifunctional (water-locking, ethylene-removing, and antibacterial) packaging biopolymer-based film (i.e., CMCGF), the characteristics and preservative effects of the packaging were investigated. Results indicated that the physicochemical (e.g., mechanical, gas/light barrier, wettability) properties of CMCGF were improved compared with the control film (i.e., CGF). CMCGF have a higher ethylene adsorption performance of 65–69 cm 3 /g STP compared with CGF (7.8 cm 3 /g STP). Cu ions released from CMCGF destroyed the cell wall and membrane, resulting in the death of bacteria, and the antibacterial efficiency of CMCGF against E. coli and S. aureus was 97–100 % and 98–100 %, respectively. Postharvest storage experiments on tomato and winter jujube confirmed the high-efficiency preservation effect of CMCGF packaging. Therefore, CMCGF provides a multifunctional approach to extending the shelf-life of perishable products to decrease food wastage.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2025
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IF 5.6
Xiaohua Su,Wenqiang Li,Renzhao Huang,Jingting Huang,Wenyong Huang,Xide Chen,Xiaodong Wu,Jianfeng Li,Xinting Li,Deguang Qin,Yi Zhan
The course of the skull defect is long, with multiple factors involved. Traditional treatment methods are not sufficient to completely restore the skull defect in a short period. Biomaterial scaffold could replace traditional approaches to bone reconstruction. A major challenge in designing a scaffold for cranial defect repair is to be non-toxic, low swelling, biodegradable, and pro-osteogenic. Herein, a biodegradable aerogel scaffold composed of methylcellulose (MC), thiolated chitosan (TCS), Zn 2 + and nano hydroxyapatite (nHA) was designed for repairing the skull defect. MC/TCS-nHA aerogel was formed by the ionic cross-linking of hydroxyl, amino and thiol groups with Zn 2+ . The prepared scaffolds exhibited a uniform pore structure and structural stability. Interestingly, TCS was used to enhance biocompatibility and biodegradability, Zn 2+ to enhance antibacterial properties and nHA to enhance pro-osteogenicity. In vivo and in vitro tests confirmed that chitosan-based aerogel could promote macrophage polarization into the M2 phenotype and promote angiogenesis and osteogenesis. The micro-CT characterization analysis and immunofluorescence analysis after 12 weeks indicated that MC/TCS-nHA aerogel could accelerate skull defect repair. Therefore, the chitosan-based aerogel showed great promise as an implant material for medical applications involving the repair of the skull.
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产品关联
DOI
PubMed
高影响因子
Gels
·
2025
·Vol. 12
·期 1
·20
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IF 5.3
Jun An,Liyou Zheng,Shuzhen Xuan,Xinyi He,Tao Yang
As a step towards the substitution of saturated fats with camellia oil in foods, a camellia oil-based oleogel was prepared using a walnut protein isolate–chitosan (WPI-CS) composite via an emulsion template method. The preparation process, structural characteristics, and stability of the oleogel were systematically analyzed. Our findings showed that varying the ratio of WPI-CS to camellia oil (CO) effectively regulated the emulsion particle size, zeta potential, and viscosity, thereby subsequently influencing the oil-holding capacity (OHC), rheological properties, and thermal stability of the oleogel. When the WPI-CS:CO ratio was 13:7, the oleogel exhibited superior performance, including relatively high OHC, improved rheological properties, and excellent thermal stability. In addition, the OHC of the oleogel varied significantly with temperature, and high oxidative stability was observed at WPI-CS ratios such as 13:7–10:10. Application tests in cake formulations demonstrated that the oleogel has potential as a partial butter replacement. This study provides a theoretical basis for the construction of WPI-based oleogels and offers new insights for the development of healthy fat substitutes.
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MATERIALS & DESIGN
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2021
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IF 7.991
Hao Li,Feng Wang
It is imperative to develop a wound dressing that can effectively treat bacterial infection in the wound location and speed up wound healing. In this study, chitosan (CS) microspheres with a core–shell structure were prepared using high-voltage electrostatic drop technology. The antimicrobial polypeptide (PonG1) and bFGF, were encapsulated in the core of the CS microsphere. The preparation of CS microspheres was studied by changing the parameters. The surface morphology of CS microspheres and the release quantities of PonG1 and bFGF were observed and detected. In vivo experiments were conducted to study the neovascularization and anti-inflammatory abilities of bFGF and PonG1. The repair ability of CS microspheres was evaluated using a rat model of full-thickness skin wound. Both bFGF and PonG1 exhibited slow release from the CS microspheres within 14 days. In vivo experiments showed that CS microspheres containing PonG1 (3 mg/ml) and bFGF (300 μg/ml) had the best neovascularization and anti-inflammatory effects. CS microspheres containing both bFGF and PonG1 had the strongest ability to repair skin wound. CS microspheres with antimicrobial and vascularized functions can be used as dressings for the repair of hard-to-heal wounds.
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CHEMICAL ENGINEERING JOURNAL
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2018
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IF 6.735
Yixuan Chen,Jianqing Yu,Qinfei Ke,Youshui Gao,Changqing Zhang,Yaping Guo
Effective repair of bone defects mainly depends on the osteoinductivity and biocompatibility of implants. Herein, we for the first time reported the bioinspired fabrication of carbonated hydroxyapatite/chitosan (CHA/CS) nanohybrid scaffolds using CaCO 3 /CS porous scaffolds as sacrificial templates, followed by loading TWS119 drugs (CHA/CS/TWS119). After treatment with phosphate buffer solutions, the CaCO 3 /CS porous scaffolds were in situ converted into CHA/CS nanohybrid scaffolds via the dissolution-precipitation reaction. The as-formed CHA nanoplates with thicknesses of ∼20 nm and widths of 1.0–2.0 μm were perpendicular to the scaffold surfaces. The three-dimensional interconnected macropores supported the adhesion and spreading of bone mesenchymal stem cells (BMSCs), and the mesopores within the CHA nanoplates as drug delivery channels improved the drug loading-release properties. The controlled drug release of the CHA/CS/TWS119 nanohybrid scaffolds significantly enhanced the osteogenic differentiation of BMSCs by the activated GSK3β/β-catenin pathway. Moreover, rat cranial defect models revealed the enhancing effects of the CHA/CS/TWS119 scaffold on bone tissue regeneration. The micro-CT, haematoxylin and eosin (H&E) staining, Van Gieson (VG) staining, Masson staining and double fluorochrome labelling results demonstrated that more new bone tissues were formed in the CHA/CS/TWS119 nanohybrid scaffolds than the control group without loading TWS119. Therefore, the CHA/CS/TWS119 nanohybrid scaffold is a promising therapeutic material for bone tissue regeneration.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2020
·
IF 2.69
Chaochen Zhao,Yang Li,Gongze Peng,Xiongxin Lei,Guifeng Zhang,Yi Gao
A bioreactor filled with functional hepatocytes is a crucial portion of the bio-artificial liver device. However, it is a difficult task to maintain sufficient cell quantity and active hepatocellular function. In this work, we developed a promising scaffold for hepatocyte culture by coating porcine liver extracellular matrix (ECM) on chitosan (CTS) fabrics. Porcine Liver was decellularized using 1% Triton X-100. Solubilized liver ECM was immobilized on CTS fibers surface through cross linking of ECM and CTS with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Then the scaffold was characterized by Fourier transformed infrared spectroscopy in attenuated total reflection mode (ATR–FTIR), X-photoelectron spectroscopy (XPS) and water contact angle measurement. The efficacy of modified scaffolds to maintain C3A hepatocytes adhesion, proliferation, bioactivity and functionality in vitro was detected. FTIR spectra and XPS demonstrated the presence of ECM coating on CTS fabric surface. Covalently attached coating significantly improved the binding efficiency between ECM and CTS fabrics, in comparison to the coating by physical absorption. Furthermore, C3A hepatocytes cultured on coated scaffolds showed enhanced cell bioactivity and liver-specific function, such as albumin secretion and urea synthesis, compared with those cultured on untreated scaffolds(p < 0.05). As a promising hepatocyte culture carrier, the ECM coated CTS fabrics could be applied in the biological artificial liver reactor.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Zhuqing Wan,Qinyuan Dong,Xiaodong Guo,Xiaoqiang Bai,Xiao Zhang,Ping Zhang,Yunsong Liu,Longwei Lv,Yongsheng Zhou
Delayed inflammatory reaction and poor osteogenesis are the two main causes of failure for bone-defect healing. Accordingly, in the present study, a dual-responsive hydrogel composite was successfully fabricated in which near-infrared (NIR)-light-responsive polydopamine-coated magnesium‐calcium carbonate microspheres are incorporated into a thermo-responsive hydroxybutyl chitosan hydrogel to provide sequential delivery of the anti-inflammatory drug aspirin and osteogenic bone morphogenetic protein 2 (BMP-2). By initially releasing aspirin rapidly, the hydrogel composite efficiently ameliorates early-stage inflammatory reaction and promotes transition to the regenerative phase. Then, the hydrogel composite allows NIR-light-responsive release of BMP-2, which maximizes its osteoinductive effects. Using an SD rat calvaria-defect model, the sequential and controllable release achieved by the hydrogel is demonstrated to promote new-bone formation. Thus, the current study provides an efficient alternative strategy for developing multifunctional therapeutic biomaterials for bone tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Qun Zheng,Deqiang Qin,Ruifei Wang,Wenjuan Yan,Weihua Zhao,Shigang Shen,Suqing Huang,Dongmei Cheng,Chen Zhao,Zhixiang Zhang
Botanical pesticides are biological pesticides that are environment friendly. However, their instability and short persistence limit their application. In this study, pH sensitive chitosan based rotenone (Rot) nanoparticles (CS/CMCS/Rot-NPs) were prepared using chitosan and carboxymethyl chitosan to take advantage of the acidic nature of the red fire ant midgut. Chitosan based nanoparticles showed photoprotective and slow sustained release effects on Rot and significantly increased the insecticidal activity of Rot against red fire ants. The 24–96hLC 50 of CS/CMCS/Rot-NPs against red fire ants was 3.28–6.84 fold that of Rot. The CS/CMCS/Rot-NPs significantly reduced the venom alkaloid content of red fire ants and their living environment and weakened their survival by increasing their survival cost in the ecological environment. Nanotechnology combined with botanical pesticides can be used as a novel, safe, effective, and ecofriendly method to control red fire ants.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Ronglu Zhang,Yuanyuan Li,Min Zhou,Cong Wang,Pengcheng Feng,Wenjun Miao,He Huang
The fact that increasing antibiotic resistance of pathogenic bacteria and a lack of new potent broad-spectrum antibiotics call for the development of alternative approaches for treating infectious diseases. With the merits of great efficacy, safety, and facile implementation, antibacterial photodynamic therapy (APDT) represents an attractive modality for this purpose. Here, we report that the newly fabricated photodynamic chitosan nano-assembly, designated CS-Ce6, could synergistically kill antibiotic-resistant bacteria with superior potency to vancomycin. CS-Ce6 nano-assembly, obtained from covalent conjugate of chlorin e6 (Ce6) with chitosan, exhibited strong association with bacteria, thus altering their morphologies and mediating great delivery efficiency of Ce6. Upon light irradiation, localized generation of singlet oxygen by CS-Ce6 nano-assembly has a remarkable bactericidal effect toward both drug-resistance Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Acinetobacter baumannii, which was greater than that the free Ce6 and antibiotics had. We also confirmed that APDT-treated MRSA neither developed resistance to APDT nor altered their resistance to methicillin. Our in vivo studies demonstrated that the CS-Ce6 nano-assembly had comparable therapeutic efficacy with vancomycin in MRSA-infected mice. These results suggest that APDT by photodynamic chitosan nano-assembly hold great potential in combating antibiotic-resistant bacteria and hopefully in reducing the need of antibiotics in the future.
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