Acta Biomaterialia
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2021
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IF 8.947
Kesi Wang,Linping Jiang,Liyan Qiu
L-arginine (L-Arg) is an important nitric oxide (NO) donor, and its exploration in NO gas therapy has received widespread attention. Application of nano-platforms that can efficiently deliver L-Arg and induce its rapid conversion to NO becomes a predominant strategy to achieve promising therapeutic effects in tumor treatment. Herein, an enhanced nano-vesicular system of ternary synergistic treatment combining NO therapy, photodynamic therapy (PDT) along with mild photothermal therapy (MPTT) was developed for cancer therapy. We integrated photosensitizer PEGylated indocyanine green (mPEG-ICG) into polyphosphazene PEP nano-vesicles through co-assembly and simultaneously encapsulated NO donor L-Arg into the vesicle center chambers to form mPEG-ICG/L-Arg co-loaded system IA-PEP. The unique nanostructure of vesicle provided considerable loading capacity for mPEG-ICG and L-Arg with 15.9% and 17.95% loading content, respectively, and efficiently prevented mPEG-ICG and L-Arg from leaking. Significantly, the reactive oxygen species (ROS) was produced by IA-PEP under 808 nm laser irradiation to perform PDT against tumors, which concurrently reacted with L-Arg to release NO and arouse gas therapy effectively. Moreover, the mild heat produced by IA-PEP could exhibit cooperative anti-tumor effect with minimal damage. As a consequence, in vivo antitumor investigation on nude mice bearing xenograft MCF-7 tumors verified the potent anti-tumor efficacy of IA-PEP under 808 nm laser irradiation with complete tumor elimination. Taken together, the IA-PEP nano-vesicle system designed in this work may provide a promising treatment paradigm for synergistic cancer treatment. Statement of significance Nitric oxide (NO) gas therapy has drawn widespread attention due to its “green” treatment paradigm with negligible side effects. L-arginine (L-Arg) is an important NO donor. However, how to efficiently deliver L-Arg and induce NO generation remains a big challenge since L-Arg is a water-soluble small molecule . Herein, we developed a nano-vesicle system IA-PEP to integrate photosensitizer PEGylated indocyanine green and L-Arg with high loading content and to produce a ternary synergistic treatment combining NO therapy, photodynamic therapy (PDT) along with mild-temperature photothermal therapy (MPTT) under 808 nm laser irradiation. The in vivo investigation on nude mice bearing xenograft MCF-7 tumors verified its potent anti-tumor efficacy with complete tumor elimination.
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Advanced Materials Interfaces
·
2023
·Vol. 11
·期 7
·2300851
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IF 5.4
Ling Huang,Zheyuan Du,Yanhong Tian,Jiangman Sun,Jinglong Li,Yue Xing,Xiubing Liang,Xuejun Zhang
With recent progress in 2D materials, Ti 3 C 2 T x MXene featured high metallic electrical conductivity, high electromagnetic interference shielding effectiveness, and super in-plane stiffness, exhibits unique advantages in many fields, but is rarely applied as a sizing agent in long-time continuous processing of carbon fiber sizing because of its poor ambient stability in aqueous suspension. Herein, a new strategy to chemically encapsulate the reactive sites of MXene nanosheet with l -cysteine for restricting the attacking of water and/or oxygen in aqueous suspension is proposed. Based on the ultra-antioxidation, polarity, and electrical properties of l -cysteine-encapsulated Ti 3 C 2 T x MXene (LC-MX) nanosheet, the LC-MX, even if aging for weeks in aqueous suspension, can be deposited on the surface of high-modulus carbon fiber (HMCF) uniformly via the electrophoretic deposition assisted sizing. Benefiting from the enhanced surface energy, wettability, and roughness of LC-MX-sized HMCF (HMCF-LCMX) relative to that of unsized one (U-HMCF), the interfacial property of HMCF-LCMX/epoxy (EP) composites is also improved, for which the interlayer shear strength (ILSS) of the composite reached 88.5 MPa, 52.8% higher than that of U-HMCF/EP composite (57.9 MPa). This work makes an essential step toward the application of ultra-stable MXene nanosheet suspension in large-scale continuous carbon fiber sizing.
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TALANTA
·
2020
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IF 5.339
Ze Zhang,Yingcong Zhang,Hongwei Yu,Shengzhong Rong,Hongmin Gao,Lingqiang Meng,Jianmin Dai,Hongzhi Pan,Dong Chang
A novel sensitive electrochemical immunosensor has been designed for determination of tacrolimus (Tac) based on spherical carrier amplification strategy. In this work, a spherical signal carrier was prepared by conjugating gold nanorods (AuNRs) functionalized L-cysteine ( [email protected] ) onto polystyrene nanoparticles (PS) to form a nanostructure, greatly increasing the amount of loaded capture antibodies. The PS was a stable spherical functional polymer with large specific surface area and was labeled with [email protected] via coupling reagent to increase active groups, biocompatibility and conductivity . The capture antibodies could be attached on the PS- [email protected] via linkage reagent glutaraldehyde (GA). Furthermore, single-layer molybdenum disulfide (MoS 2 ) fixed by chitosan were utilized to construct the base of this immunosensor, increasing the carrying capacity and stability of the electrode. After electrochemical reduction , the conductivity and electron transfer rate of MoS 2 were obviously improved, which offered a platform for this immunosensor and further amplified the signal. Under the optimized conditions, the proposed immunosensor revealed a linear Tac-concentration behavior from 1.0 to 30 ng mL −1 with a detection limit of 0.17 ng mL −1 (S/N = 3). The immunoassay was successfully applied to the quantification of Tac in serum samples with acceptable precision. The results indicated that a potentially analytical platform may be used to recognize the concentration of other drugs.
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Materials Science & Engineering C-Materials for Biological Applications
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2020
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IF 5.88
Lina Ding,Yi Chang,Pengfei Yang,Weihua Gao,Mengmeng Sun,Yameng Bie,Lin Yang,Xiaoming Ma,Yuming Guo
Photothermal therapy (PTT) can take advantage of the photothermal effects of photothermal agents to acquire the energy from laser irradiation and convert it into heat. This can intensively elevate the temperature of the surrounding environment to directly destroy the cancer cells. It is expected that PAs with strong absorption in near infrared (NIR) range might possess the ideal tissue-transparent feature and minimal damage to surrounding healthy tissues, beneficial to the practical application. Herein, well-dispersed L-cysteine modified MoS 2 (MoS 2 -Cys) nanospheres with the average diameter of about 422 nm and strong NIR adsorption were successfully synthesized through a facile method. The as-prepared MoS 2 -Cys nanospheres are composed of nanosheets with the average thickness of about 13.7 nm. The photothermal conversion efficiency of the as-prepared MoS 2 -Cys nanospheres with the very low concentration of 50 μg/mL was determined to be 35% when exposed to 808 NIR laser at the power density of 0.8 W/cm 2 , much higher than those of the previous reports with same dose and power density. MoS 2 -Cys nanospheres possessed the good photothermal ablation effect to significantly inhibit the growth of tumor in vivo . Furthermore, MoS 2 -Cys nanospheres did not exhibit detectable side effects, suggesting their good in vitro and in vivo biocompatibility.
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JOURNAL OF PROSTHETIC DENTISTRY
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2026
·
IF 5.6
Hao Yang,Hongyu Gao,Xianju Xie,Yuxing Bai
Statement of problem Polymethyl methacrylate (PMMA) has been commonly used for dental appliances. However, it lacks inherent antibacterial properties, thus increasing the risk of oral diseases such as gingival inflammation and denture stomatitis. Given that PMMA-based appliances should offer prolonged intraoral service, the functionalization of PMMA with antibacterial activity and mechanical integrity after water aging is crucial to improve its clinical applicability. Purpose The purpose of this in vitro study was to evaluate the mechanical characteristics, protein resistance, and antibacterial efficacy of a previously designed novel antibacterial PMMA resin co-incorporating ε-poly- L -lysine (ε-PL) and 2-methacryloyloxyethyl phosphorylcholine (MPC) after 3 months of water aging in artificial saliva to simulate oral conditions. Material and methods Four experimental groups were established based on the previous research: PMMA (Control), PMMA containing 1.5% ε-PL, PMMA containing 1.5% MPC, and PMMA containing 1.5% ε-PL and 1.5% MPC. Specimens underwent 3 months of water aging in artificial saliva at 37 °C with weekly solution replacement. The flexural strength ( n =12) was determined by a 3-point bend test, and surface topography and roughness were determined via atomic force microscopy ( n =6). Protein adsorption was determined by the Micro bicinchoninic acid (BCA) assay, and antibacterial efficacy was measured via live or dead fluorescence staining and metabolic activity assay ( n =6). Statistical evaluation used Shapiro–Wilk testing followed by ANOVA and Tukey HSD or Welch and Games–Howell tests (α=.05). Results The flexural strength, surface topography, and roughness demonstrated no significant intergroup differences ( P >.05), indicating that the ε-PL and MPC modifications did not compromise mechanical performance relative to the Control after water aging. In addition, the co-incorporation of 1.5% ε-PL and MPC enhanced the antibacterial efficacy of PMMA without increasing protein adsorption compared with the Control ( P <.05). Conclusions This novel PMMA resin showed antibacterial efficacy without compromising mechanical integrity and protein adsorption after 3 months of water aging, suggesting its potential for dental clinical application.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Min Fan,Jianxu Zhang,Sen Yin,Jingya Li,Ting Gao,Li Li,Yanyan Zhou,Hongfeng Li
Abstract l-Cysteine (Cys) levels are linked to many health issues and serve as critical diagnostic criteria for various diseases. However, their highly specific recognition and determination from complex biological matrices remain challenging due to interference from biothiol-containing analogs, such as homocysteine (Hcy) and glutathione (GSH). Herein, by strategically positioning eight aldehyde groups at the periphery of a dinuclear Eu(III) helicate, we achieved the specific determination of l-Cys via an induced negative circularly polarized luminescence (CPL) signal (gmax ≈ −1.22, 595 nm). 1H NMR and ESI-MS combined with single-crystal X-ray crystallography and DFT calculations revealed that the size match between the periphery pocket in the helicate and the thiazolidine ring formed by l-Cys and aldehyde cyclization contributed to this CPL response, which creates a large energy difference (∼37.6 kcal/mol) between diastereoisomers and a M-helical preference. While the L-Hcy only resulted in a weak and positive signal (glum ≈ 0.15) due to the larger ring size, the GSH was CPL silent due to the non-cyclization reaction. Benefiting from the large glum value, the probe exhibited the high sensitivity for l-Cys in water or bovine serum albumin (BSA), with a detection limit lower than 8.11 × 10–7 M. Additionally, to facilitate commercial applications, a “test strip-like” hydrogel hybrid film was prepared, which still retains sensing capability to l-Cys. This work therefore provides a strategy for l-Cysteine detection by virtue of induced helical chirality of metallosupramolecules and strong lanthanide CPL.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2023
·
IF 4.1
Cheng-Hai Yan,Fang-Hui Chen,Yu-Lu Yang,Li-Wei Shen,Xiao-Meng Xun,Zhi-Ang Zhang,Yu-Fan Zhan,You Shuai,Jun Wang
BACKGROUND With the requirements of environmental, cost and economic sustainability, new sources of alternative proteins in the livestock industry are receiving increasing attention. Mulberry ( Morus alba L.) leaves are a unique feed resource due to their high protein content and large availability. Therefore, mining sustainable protein suitable for the animal husbandry industry in sericulture resources could achieve a win-win situation. RESULTS The protein content in mulberry leaves is 232.10-386.16 g·kg -1 , and the mean value of crude fat content is 43.76±8.48 g·kg -1 , which has the advantages of protein content and energy. In addition, the average content of phytic acid in mulberry leaves is only 1.88±0.56 g·kg -1 , which makes it not inhibited in terms of nutrient absorption. Meanwhile, the digestibility of protein was Bean pulp> Sample 8≈ Alfalfa≈ Sample 13≈ Cottonseed meal> Fish meal, and the β -turn and particle size of mulberry leaf protein are more conducive to digestion in vitro . Furthermore, the protein of sample 13 had the richest essential amino acids (252.00 g·kg -1 ) and the highest essential amino acid index (EAAI), which was superior to conventional feed protein. In addition, the partial substitution of mulberry leaf protein (15%) significantly increased the EAAI value of conventional feed protein. However, to balance nutrition, it is necessary to combine mulberry leaf protein with other proteins to further broaden its application field. CONCLUSION Mulberry leaves are a new source of feed protein, which helps to alleviate the two major problems of mulberry resource surplus and feed protein resource shortage. This article is protected by copyright. All rights reserved.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Yaojin Ge,Xiaofeng Fang,Yun Zhang,Shengfeng Peng,Suyun Lin,Lei Zhou,David Julian McClements,Wei Liu
Thymol forms eutectic solvents with lauric acid under appropriate mixing ratios. These eutectic solvents were shown to have a lower melting point, weaker irritatibility, and stronger antibacterial activity than pure thymol. However, the application of these eutectic solvents is currently limited by their poor water solubility and chemical instability. For this reason, thymol/lauric acid eutectic solvents were loaded into casein nanoparticles (ThyLA-NPs) to improve their solubility and stability characteristics. The physicochemical stability, antibacterial activity, and potential application for meat (pork) preservation of the ThyLA-NPs was then compared to that of thymol casein nanoparticles (Thy-NPs). The results showed that ThyLA-NPs exhibited long-term sustained release, better thermal stability, and better storage stability due to the higher hydrophobicity of the eutectic solvents. Moreover, the ThyLA-NPs showed higher antibacterial activity against L. monocytogenes . The pork preservation study indicated that the ThyLA-NPs were more efficient at inhibiting microbial growth and lipid antioxidation than the Thy-NPs, which extended the shelf life of the pork. These results may promote the application of thymol in the food industry to control food safety and quality.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2026
·
IF 5.7
Xiaoyu Yang,Jiawei Zhang,Jinyu Yang,Junhua Wang,XiaoXiao Jiang,Jianzhi Zhao,Xiangyan Chen,Leilei Chen,Qingxin Zhou
E. cristatum drives metabolic transformation during solid-state fermentation. • Multi-omics reveals microbial mechanisms underlying AVL fermentation. • Metaproteomics identified enzymes involved in plant substrate transformation. • Fermentation reshaped non-volatile metabolites and aroma profiles of AVL. • Protein–metabolite associations reveal functional modules in fungal fermentation. Fermentation by food microorganism provides an effective approach for transforming plant-based substrates, yet the microbial mechanisms underlying these biochemical processes remain insufficiently understood. In this study, Eurotium cristatum -mediated solid-state fermentation of Apocynum venetum L. tea (AVL) was investigated at 2, 4 and 6 days using an integrated multi-omics approach and sensory-related analyses. Fermentation decreased bitterness and astringency of AVL infusions, accompanied by reductions in total polyphenols and flavonoids. Untargeted metabolomics identified 41 core differential non-volatile metabolites, predominantly fatty acyls, glycerophospholipids, steroids, phenolics, and terpenoids. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment highlighted phenylpropanoid-, flavonoid-, and isoflavonoid-related pathways. Volatile profiling identified 16 aroma-active compounds. Changes in aldehydes, ketones, alcohols, and esters contributed to fermentation-associated aroma characteristics. Metaproteomic analysis identified 4614 E. cristatum proteins, including 1838 differential proteins enriched in functional categories related to catalytic activity, oxidoreductase activity, glycosyl bond hydrolase activity, carbohydrate metabolism, and secondary metabolism. Integrated multi-omics analysis showed that non-volatile metabolite remodeling was associated with multiple microbial functional modules related to carbon metabolism, redox reactions, and secondary metabolism while fatty acid and amino acid metabolism were associated with the formation of characteristic aroma-active compounds. Overall, E. cristatum -mediated fermentation of AVL represents a growth-coupled, enzyme-associated biotransformation process in which coordinated microbial metabolic activities contribute to metabolites remodeling and the development of characteristic taste and aroma properties. These findings provide a mechanistic basis for understanding E. cristatum -mediated transformation of plant substrates and offer insights into the formation of characteristic quality attributes during fermented tea production.
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JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY
·
2023
·
IF 2.3
Qinghao Sun,Yichun Peng,Shaopeng Fu,Pangang Li,Hongmei Ma,Zheng Fang,Tianfu Ma,SanShan Du,Zezhou Liang,Jianfeng Li
L-Glutathione reduced (GSH) is a small molecule peptide found in large quantities in living organisms, particularly in animal liver cells. Its unique structure gives it great potential to act as an environmentally friendly and efficient corrosion inhibitor. The inhibition properties of GSH for mild steel in simulated concrete pore solutions (SCPS) containing chlorine was investigated by electrochemical tests and SEM-EDS surface analysis. According to the experimental results, GSH mainly blocked the cathodic corrosion reaction and has a corrosion inhibition efficiency of up to 92.3% at room temperature, enhancing the corrosion resistance of mild steel. The isothermal adsorption results indicate that the adsorption behavior of GSH on the surface follows the Langmuir isothermal adsorption model. Both quantum chemical calculations (QC) and molecular dynamics simulations (MD) show that GSH can be effectively adsorbed onto mild steel substrates, thus protecting the surface from erosion. Finally, the results of the above experiments and theoretical calculations are combined to elucidate the inhibitive mechanism: GSH molecules binds to the iron surface via the sulfur, nitrogen and oxygen atoms in the molecule, thus adsorbing to the iron surface in a tiled manner and forming a protective film to prevent the corrosive material from contacting the iron surface.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
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IF 3.4
Xin Zhang,Yuhan Jiang,Na Guo,Yifan Ding,Jingting Feng,Chenyang Miao,Yanni Lv
The SNAP-tag-epidermal growth factor receptor (SNAP-tag-EGFR) cell membrane chromatography (CMC) model is a powerful tool for investigating ligand-receptor interactions and screening active ingredients in traditional Chinese medicine. Most tyrosine kinase inhibitors (TKIs) target epidermal growth factor receptors. However, TKIs associated with significant side effects and drug resistance must be addressed immediately. Therefore, there is an urgent need to develop new TKIs with high efficiency and low toxicity. Because of its low toxicity and side effects, traditional Chinese medicine has been widely employed to treat various diseases, including cancer. Hence, this study aimed to use the SNAP-tag-EGFR/CMC-high-performance liquid chromatography-mass spectrometry (HPLC-MS) two-dimensional system model as the research tool to screen and identify potential EGFR antagonists from the Chinese medicine Silybum marianum (L.) Gaertn . The applicability of the system was verified using the positive control drug osimertinib. Four potential EGFR antagonists were screened from the Chinese medicine Silybum marianum (L.) Gaertn. . They were identified as silydianin, silychristin, silybin, and isosilybin. Additionally, their pharmacological activity was preliminarily verified using a CCK-8 assay. The kinetic parameters of the four active ingredients interacting with EGFR and their binding modes with EGFR were analyzed using nonlinear chromatography (NLC) and molecular docking. This study identified silydianin, silychristin, silybin, and isosilybin from Silybum marianum (L.) Gaertn. and verified their potential antitumor effects on EGFR.
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FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Xiang Ren,Xinyu Zhang,Peizi Sun,Junxin Lin,Yuying Zhang,Dongmei Li
Antarctic krill suffers from severe water loss after heating, and its quality deteriorates, so it is in urgent need of a green and healthy improver. In this paper, the effects of L-arginine (L-Arg) soaking on the modification of the quality of heat-treated Antarctic krill and the structure of myofibrillar proteins (MPs) in Antarctic krill were investigated. The results showed that L-Arg had an ameliorating effect on heat-treated krill in a concentration-dependent relationship. The water-holding capacity of L-Arg-soaked krill was 1.41 times higher than that of sodium tripolyphosphate (STPP) at an equivalent concentration (80 mM). At 120 mM L-Arg soaked, L * and hardness of krill decreased to 58.31 and 334.81 g, while resilience and moisture content increased to 0.47 and 85.29% after heating, respectively. The scanning electron microscopy (SEM) results revealed that the tissue state of the pH-corrected groups was better than the control, but not as well as that of the pH-uncorrected groups. pH and the guanidinium group in L-Arg both played roles in promoting the transition of MPs from disordered to ordered secondary structures. This transition reduced the exposure of hydrophobic and sulfhydryl groups in MPs, inhibited the protein aggregation and increased the solubility of MPs to 71.61%, which ultimately improved the quality of heat-treated krill. It is worth noting that the pH effect had a primary influence on the observed effects, while the guanidinium group made a secondary contribution. These results could broaden the potential application of L-Arg as an improver of the quality of heat-treated krill.
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