Frontiers in Pharmacology
·
2020
·
IF 4.225
Huang Mei-Zhou,Zhang Zhen-Dong,Yang Ya-Jun,Liu Xi-Wang,Qin Zhe,Li Jian-Yong
Aspirin eugenol ester (AEE) is a new potential pharmaceutical compound possessing anti-inflammatory, anti-cardiovascular disease, and antioxidative stress activity. The pharmacological activities of AEE are partly dependent on its regulation of cell apoptosis. However, it is still unclear how AEE inhibits cell apoptosis on the basis of its antioxidative stress effect. This study aimed to reveal the vascular antioxidative mechanism of AEE in response to H2O2-induced oxidative stress in HUVECs and paraquat-induced oxidative stress in rats. In the different intervention groups of HUVECs and rats, the expression of ASK1, ERK1/2, SAPK/JNK, and p38 and the phosphorylation levels of ERK1/2, SAPK/JNK, and p38 were measured. The effects of ASK1 and ERK1/2 on the anti-apoptotic activity of AEE in the oxidative stress model were probed using the corresponding inhibitors ASK1 and ERK1/2. The results showed that in the HUVECs, 200 μM H2O2 treatment significantly increased the phosphorylation of SAPK/JNK and the level of ASK1 but decreased the phosphorylation of ERK1/2, while in the HUVECs pretreated with AEE, the H2O2-induced changes were significantly ameliorated. The findings were observed in vitro and in vivo. Moreover, inhibition of ASK1 and ERK1/2 showed that ASK1 plays a vital role in the protective effect of AEE on H2O2-induced apoptosis. All findings suggested that AEE protects the vascular endothelium from oxidative injury by mediating the ASK1 pathway.
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高影响因子
Experimental and Therapeutic Medicine
·
2017
·Vol. 14
·期 4
·2937-2942
·
IF 1.261
Lixiao Pan,Fengyun Yang,Caixia Lu,Changxin Jia,Qing Wang,Kexue Zeng
The purpose of this investigation was to determine the effects of sevoflurane on rats with ischemic brain injury and to determine the potential role of the TREK-1 channel in this process. Normal rats were randomly divided into three groups: Sham operation, sevoflurane anesthesia or chloral hydrate anesthesia group, an additional group of TREK-1 knockout rats were also studied. Semi-quantitative PCR and western blot analysis confirmed the lack of TREK-1 expression in the brain of TREK-1 knockout rats. The thread-tie method was used to establish middle cerebral artery occlusion (MCAO) model to induce cerebral ischemic brain injury. All rates were treated for 4 days prior to ischemia (for 2 h) followed by a 24 h reperfusion period. Physiological indexes of rats in each group both prior to and after surgery showed no statistical difference (P>0.05). Neurological function was scored both before (no statistical difference) and after surgery where it was found to be significantly better (lower score) in the sevoflurane anesthesia group than in chloral hydrate anesthesia and TREK-1 knockout groups (P<0.01). The area of cerebral infarction was measured by triphenyl tetrazolium chloride staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay to detect the apoptosis of brain cells. TTC staining showed different degrees of cerebral infarction in the various groups; the area of cerebral infarction in sevoflurane anesthesia group was significantly lower than that in chloral hydrate anesthesia and TREK-1 knockout groups (P<0.01). TUNEL assay showed that the number of TUNEL-positive cells was significantly lower in sevoflurane anesthesia group than in TREK-1 knockout and chloral hydrate anesthesia groups (P<0.01). In conclusion, results from this investigation showed that sevoflurane can protect the nerve function of rats with cerebral ischemic brain injury possibly by affecting the expression of proteins involved in the TREK-1 signaling pathway.
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EXPERIMENTAL ANIMALS
·
2021
·
IF 2.319
Lili TAN,Limin LIU,Jian YAO,Chenghao PIAO
Myocardial ischemia-reperfusion (I/R) injury is a common complication following reperfusion therapy that involves a series of immune or apoptotic reactions. Studies have revealed the potential roles of miRNAs in I/R injury. Herein, we established a myocardial I/R model in rats and a hypoxia/reoxygenation (H/R) model in H9c2 cells and investigated the effect of miR-145-5p on myocardial I/R injury. After 3 h or 24 h of reperfusion, LVESP, EF, and FS were obviously decreased, and LVEDP was increased. Meanwhile, I/R induced an increase in myocardial infarction area. Moreover, a decrease in miR-145-5p and increase in NOH-1 were observed following I/R injury. With this in mind, we performed a luciferase reporter assay and demonstrated that miR-145-5p directly bound to NOH-1 3’ UTR. Furthermore, miR-145-5p mimics decreased the levels of TNF-α, IL-1β, and IL-6 via OGD/R stimulation. Upregulation of miR-145-5p increased cell viability and reduced apoptosis accompanied by downregulation of Bax, cleaved caspase-3, cleaved PARP and upregulation of Bcl2. In addition, miR-145-5p overexpression increased SOD activity and reduced ROS and MDA content under OGD/R stress. Notably, NOH-1 could significantly abrogate the above effects, suggesting that it is involved in miR-145-5p-regulated I/R injury. In summary, our findings indicated that miR-145-5p/NOH-1 has a protective effect on myocardial I/R injury by inhibiting the inflammatory response and apoptosis.
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高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2019
·
IF 5.657
Zhen Wang,Shi-peng Wang,Qun Shao,Pei-feng Li,Yue Sun,Lan-zi Luo,Xiu-qing Yan,Zi-yi Fan,Juan Hu,Jing Zhao,Peng-zhou Hang,Zhi-min Du
Brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase B (TrkB) pathway is associated with ischemic heart diseases (IHD). 7,8-dihydroxyflavone (7,8-DHF), BDNF mimetic, is a potent agonist of TrkB. We aimed to investigate the effects and the underlying mechanisms of 7,8-DHF on cardiac ischemia . Myocardial ischemic mouse model was induced by ligation of left anterior descending coronary artery . 7,8-DHF (5 mg/kg) was administered intraperitoneally two days after ischemia for four weeks. Echocardiography , HE staining and transmission electron microscope were used to examine the function, histology and ultrastructure of the heart. H9c2 cells were treated with hydrogen peroxide (H 2 O 2 ), 7,8-DHF or TrkB inhibitor ANA-12. The effects of 7,8-DHF on cell viability , mitochondrial membrane potential (MMP) and mitochondrial superoxide generation were examined. Furthermore, mitochondrial fission and protein expression of mitochondrial dynamics (Mfn2 [mitofusin 2], OPA1 [optic atrophy 1], Drp1 [dynamin-related protein 1] and Fis-1 [fission 1]) was detected by mitotracker green staining and western blot , respectively. 7,8-DHF attenuated cardiac dysfunction and cardiomyocyte abnormality of myocardial ischemic mice. Moreover, 7,8-DHF increased cell viability and reduced cell death accompanied by improving MMP, inhibiting mitochondrial superoxide and preventing excessive mitochondrial fission of H 2 O 2 -treated H9c2 cells. The cytoprotective effects of 7,8-DHF were antagonized by ANA-12. Mechanistically, 7,8-DHF repressed OMA1-dependent conversion of L-OPA1 into S-OPA1, which was abolished by Akt inhibitor . In conclusion, 7,8-DHF protects against cardiac ischemic injury by inhibiting the proteolytic cleavage of OPA1. These findings provide a novel pharmacological effect of 7,8-DHF on mitochondrial dynamics and a new potential target for IHD.
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高影响因子
Anti-Cancer Agents in Medicinal Chemistry
·
2022
·Vol. 22
·期 17
·3009-3024
·
IF 2.527
Zhu Yuting,Wang Huai,Yu Mingjun,Li Chao,Meng Xiaoming,He Meng,Yao Risheng
Background: GRPR is over-expressed in cancer cells and is a potential drug target for the treatment of cancer. PD176252, as the most representative non-peptide inhibitor of GRPR, can inhibit the growth of cancer cells, but its low selectivity to cancer cells and normal cells limits
its further application. Objective: The aim of this study was to design and synthesize novel GRPR inhibitor with stronger anti-cancer activity and higher affinity with GRPR than the lead compound PD176252. Methods: A series of 1, 3, 4-oxadiazole derivatives as PD176252 analogues
(4a-4j, 6a-6q) were synthesized and their cytotoxic activity was investigated on four cancer lines with high expression of GRPR (gastric (HGC-27), colon (HCT- 116), prostate (PC-3), and lung (A549)) and one human cell line (gastric mucosal epithelial (GES-1)) by MTT assay. Flow cytometry analysis
and Western Blot were used to determine whether the compound induced programmed apoptosis of cancer cells. Competitive binding experiment was used to verify the affinity between GRPR and the optimal compound. Results: Compound 6m exhibited significant growth inhibition on all tested cancer
cell lines, especially gastric cancer cells (HGC-27 cellular IC50 0.37 ± 0.04μM). Also, the selectivity of 6m to HGC-27 was much higher than that of PD176252. Flow cytometric analysis and Western Blot proved that 6m significantly promoted the apoptosis of HGC-
27 cells. Moreover, competitive binding experiment confirmed the close binding of 6m with GRPR, which indicated 6m with a higher affinity than lead compound PD176252. Conclusion: Our results suggested that 6m, as a novel GRPR inhibitor, had a higher affinity with GRPR and potential anti-cancer
effect than PD176252, which can be used as a template for further optimization.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Wenjie He,Wei Xu,Zhigang Li,Yansheng Sun,Jingzeng Gu,Xinxin Cao,Kangsheng Huang,Min Zhang,Jiangmin Jiang
The large-scale development of low-cost silicon monoxide (SiO) anode is limited due to its low initial Coulombic efficiency (ICE) and poor cycle stability. Herein, the Ge x SiO (1-x) (0
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CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Youbing Zhu,Ketao Shi,Tao Zhang,Nianming Jiao,Hui Wang
One-dimensional microporous zeolite, EU-1, exhibits more favorable selectivity of propylene in cracking of petroleum components. However, it faced the problem of low catalytic activity for the poor utilization of active sites. In this work, dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (TPOAC) was used to functionalize protozeolitic units and inhibit the excessive growth of crystals by the long hydrophobic alkyl chain. The introduction of TPOAC decreased the crystal sizes into nanoscale, enhanced the mesoporous volume of EU-1 zeolite, and exposed more external acid sites. Moreover, TPOAC could interact with Al species, and in this case, fewer Al atoms were incorporated into the zeolite framework, reducing the acid strength and amount. Intelligent gravitation analyzer test indicated that the zeolite prepared with the presence of 0.30 g TPOAC exhibited the largest accessible volumes in n-hexane adsorption, and the highest accessibility of acid sites, which synergistically promoted cracking of n-hexane for the production of light olefins.
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Frontiers of Chemical Science and Engineering
·
2024
·Vol. 18
·期 2
·1-13
·
IF 4.3
Zhang Feng,Xu Shuainan,Geng Xiumei,Shan Meixia,Zhang Yatao
Polymers of intrinsic microporosity shows great potential for dye adsorption and magnetic Fe 3 O 4 are easy to be separated. In this work, hydrolyzed polymers of intrinsic microporosity-1/Fe 3 O 4 composite adsorbents were prepared by phase inversion and hydrolysis process for cationic dye adsorption. The chemical structure and morphology of the composite adsorbents were systematically characterized by several characterization methods. Using methylene blue as the target dye, the influences of solution pH, contact time, initial dye concentration, and system temperature on the methylene blue adsorption process were investigated. The incorporation of Fe 3 O 4 particle into hydrolyzed polymers of intrinsic microporosity-1 endow the adsorbent with high magnetic saturation (20.7 emu·g −1 ) which allows the rapid separation of the adsorbent. Furthermore, the adsorption process was simulated by adsorption kinetics, isotherms and thermodynamics to gain insight onto the intrinsic adsorption mechanism. In addition, the composite adsorbents are able to selectively adsorb cationic dyes from mixed dyes solution. Hydrolyzed polymers of intrinsic microporosity/Fe 3 O 4 shows only a slight decrease for methylene blue adsorption after 10 adsorption/regeneration cycles, demonstrating the outstanding regeneration performance. The high adsorption capacity, outstanding regeneration ability, together with simple preparation method, endow the composite adsorbents great potential for selective removal of cationic dyes in wastewater system.
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JOURNAL OF POWER SOURCES
·
2020
·
IF 8.247
Ning An,Zhuhang Shao,Zhen Guo,Jiao Xin,Yuanyuan He,Liwen Lv,Kefeng Xie,Xiuyan Dong,Yang Zhang,Zhongai Hu
In this work, we select a novel multi-redox poly (1, 5-diaminoanthraquinone) (PDAAQ) as electroactive material and Cetyltrimethylammonium bromide (CTAB) modified graphene oxide (CGO) as conductive substrate. Benefit from the excellent dispersion of CGO in organic polymerization medium and the abundant functional groups on the surface of CGO, 1, 5-diaminoanthraquinone (DAAQ) monomers may covalently graft on the conducting substrates and realize the directional growth of PDAAQ via the in-situ chemical oxidative polymerization. In three-electrode system, the optimization of PDAAQ/CGO electrode exhibits a high specific capacitance of 760 F g −1 at 1 A g −1 and superior electrochemical stability of 90.4% after 10 000 cycles. Most importantly, the electrochemical kinetic behavior of PDAAQ/CGO electrode is studied in detail. The result indicates that the energy storage process of PDAAQ/CGO is a synergistic result of diffusion-controlled process and capacitive effects, and the latter one is dominant. According to the simulation calculation of kinetic model , the capacitive contribution ratio of PDAAQ/CGO can be as high as 80.6% at a scan rate of 50 mV s −1 . Finally, the as-fabricated asymmetric supercapacitor (ASC) can deliver an energy density up to 41.1 W h kg −1 at a power density of 1000 W kg −1 , indicating a great potential application in high performance supercapacitors .
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xin Li,Longxun Teng,Yening Ren,Rui Liu,Xiaoyuan Zhan,Haiqing Sun,Weiwei Zhang,Jianxu Ding,Huiling Zhu
Metal halide perovskite CsPbI3 quantum dots (QDs) have sparked widespread research due to their intriguing optoelectronic. However, the CsPbI3 QDs undergo inevitable aging and luminescence quenching caused by the weak binding ability of oleate (OA–)/oleylammonium (OAm+), hindering further practical application. Herein, we have realized ultrafast rejuvenation of the aged CsPbI3 QDs that have lost their photoluminescence performance based on a 1-dodecanethiol (DDT) surface ligand to restore the outstanding red light emission with a high photoluminescence quantum yield (PLQY) from 25 to 90%. Furthermore, CsPbI3 QDs with DDT surface treatment maintain a cubic phase and high PLQY value even after 35 days. The DDT ligands can form a strong bond with Pb2+ and passivate I– ion vacancies, enhancing radiative recombination efficiency and thereby improving the PLQY of the QDs. The stable yet easily accessible surface of the DDT-capped CsPbI3 QDs was successfully employed as white LEDs and exhibited considerable enhanced luminous performance, suggesting promising application in solid-state lighting fields.
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Current Computer-Aided Drug Design
·
2024
·
IF 1.5
Na An,Dongxing Zhang,Jile Xin,Xinyi Zhang,Zhijuan Zhang,Ligang Ma,Le Zhao,Huimin Wu,Weisheng Feng,Xiaoke Zheng
Background: Si-Ni-San (SNS) is the formula prescription of Traditional Chinese Medicine (TCM) with anti-depression properties, but its underlying mechanisms remain unclear. Objective: This study provides novel approaches for the study of Traditional Chinese Medicine (TCM) and offers new opportunities for exploring the pharmacological properties of SNS. Methods: The ingredients in SNS implicated in the treatment of depression were identified and studied using network pharmacology. SwissTargetPrediction and molecular docking were used to study the interaction of SNS ingredients and their targets. The protective effect of these ingredients and their cocktail in rat pheochromocytoma cells (PC12) exposed to corticosterone (Cor) were evaluated using the CCK-8 assay, Hoechst 33342 staining, 2',7'-dichlorodihydro fluorescein diacetate (H2DCFDA) staining, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and in-cell Western analysis. Results: The network pharmacology study showed that the HIF-1 signaling pathway was the most crucial pathway implicated in the anti-depressive property of SNS. MAPK1 (ERK2), MAPK3 (ERK1), AKT1, VEGFA, STAT3, and EGF were identified as hub target proteins in the HIF-1 signaling pathway. Quercetin, naringenin, licochalcone A, and kaempferol from SNS, which targeted the six proteins mentioned above, were used to create a cocktail. This cocktail exerted protective properties, decreased the oxidative stress in PC12 exposed to Cor, and successfully regulated the expressions of AKT1, p-AKT1, ERK1, ERK2, p-ERK1/2, STAT3, p- STAT3, and VEGFA induced by Cor exposure. The SwissTargetPrediction and molecular docking study showed that the cocktail may regulate the HIF-1 signaling pathway by directly binding with AKT1 and MAPK1. Conclusion: The cocktail from SNS comprised of quercetin, naringenin, licochalcone A, and kaempferol exerts anti-depression potentiality by modulating the HIF-1 signaling pathway via direct interactions with AKT1 and MAPK1.
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高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Yuyu Liu,Wenye Deng,Xianghui Chen,Yan Xue,Xuelian Bai,Pengjun Zhao,Huimin Zhang,Aimin Chang,Yongxin Xie
The perovskite CaMn x Zr (1- x ) O 3 ( x = 0, 0.05, 0.10, 0.15) (CMZO) NTC (Negative Thermal Coefficient) thermo-sensitive ceramic were prepared by the solid phase method that can be used as high temperature thermistor materials in the range of 200–1200 °C.The XRD pattern showed that CMZO is a finite solid solution and that a second phase, CaMnO 3 , will be formed after exceeding the solid solution limit (i.e., at x = 0.1). The thermal constant B value was found to decrease from 19521 K to 9217–9535 K, with a corresponding decrease in activation energy , E a , from 1.69 eV to 0.82 eV. The Nyquist plot indicates that the grain boundary resistance determines the overall complex impedance. Moreover, the aging drift rate (ΔR/R 0 ) was found to be lower than 4.3%, which is an indication of an excellent high temperature stability of the CMZO ceramic material.
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危险品化学品经营许可证（带存储）