Chemical Biology & Drug Design
·
2025
·Vol. 106
·期 1
·e70158
·
IF 3.3
Yong Zhu,Chuanmin Yi,Jin Zhao,Lei Wang,Tao Huang,Bo Xiang,Lvhai Zhang,Xiangfang He,Linke Wu
Platinum-based and pyrimidine drugs are first-line treatments for gastric cancer (GC), but their efficacy is often affected by drug resistance. High spalt-like transcription factor 4 (SALL4) expression is associated with poor prognosis, but its role in 5-fluorouracil (5-FU) resistance is not yet clear. In this study, we investigated the effect of SALL4 on 5-FU resistance in GC cells by bioinformatics analysis, real-time quantitative reverse transcription polymerase chain reaction, cell counting kit-8, colony formation assay, and western blot. The results showed that SALL4 was highly expressed in GC and significantly correlated with the fatty acid oxidation (FAO) pathway. Knockdown of SALL4 resulted in a notable attenuation of cellular proliferative capacity and heightened susceptibility to 5-FU resistance in GC cells, while overexpression of SALL4 enhanced 5-FU resistance. Rescue assays confirmed that SALL4 fostered 5-FU resistance in GC cells by enhancing FAO. Our research confirmed that SALL4 promoted the resistance of GC cells to 5-FU by enhancing the FAO pathway. This suggests that drug development targeting SALL4 may help overcome chemotherapy resistance in GC.
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Materials Chemistry Frontiers
·
2024
·
IF 6
Jia Liu,Zhiyang Wang,Xiaowei Wu,Shanyue Wei,Yiming Xie,Jing Chen,Can-Zhong Lu
Owing to the unique properties of high porosity, outstanding chemical stability, and designable structures, vast applications of covalent organic frameworks (COFs) have been explored in a wide range of areas including energy storage, catalysis, gas separation, etc. Nevertheless, from the perspective of electrochemical sensing, the conductivity of COFs is not satisfactory. Here, a two-dimensional (2D) hybrid nanosheet combining a COF, a supramolecular host, and nanomaterials was constructed by assembling cucurbit[8]uril (CB8) onto the surface of the COF, and achieved in situ growth of uniformly dispersed AuNPs with the aid of the modulation effect of CB8 to synergistically enhance the sensing performance. By taking advantage of the abundant active loading sites and large specific area of the 2D-COF, excellent conductivity of AuNPs, and host–guest binding ability of CB8, the prepared COF/CB8/AuNPs is utilized to modify the glassy carbon electrode (GCE) as an advanced electrochemical sensor for simultaneous detection of structurally similar analytes, e.g. antineoplastic agent 5-fluorouracil (5-FU) and RNA base uracil (U) using differential pulse voltammetry, achieving detection limits down to the nanomolar level (e.g. 0.037 and 0.074 μM for 5-FU and U, respectively) and the physiologically relevant linear detection range within the sub-micromolar window. Impressively, the prepared COF/CB8/AuNPs/GCE sensor is considerably robust in complicated biofluids, allowing for detecting 5-FU and U in unprocessed native synthetic urine (SU) and whole fetal bovine serum (FBS), which remained highly challenging for electrochemical sensors. This study elucidates the integration of COFs with supramolecular host systems, paving the way for the development of advanced electrochemical sensors for rapid and onsite detection in pharmaceutical diagnostic applications.
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RENEWABLE ENERGY
·
2025
·
IF 9.1
Yu Jia,Fuwei Lu,Youzhou Jiao,Hongge Tao,Jinbo Hao,Guizhuan Xu,Chao He,Xinxin Liu,Chun Chang,Yaxing Song,Binglin Chen
Solvent and catalyst are important factors in the synthesis of 5-hydroxymethylfurfural (HMF). Herein, different solvents and catalysts were selected to explore the synergistic mechanism to boost the conversion of cellulose to HMF. It was found that low-polarity aprotic (molecular polarizability index <15) and medium basicity (800 kJ/mol < protonation affinity <840 kJ/mol) solvents, such as 1,4-dioxane, tetrahydrofuran (THF) and methyl isobutyl ketone (MIBK) were optimal for homogeneous Brønsted acid-catalyzed HMF production from cellulose. The highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) energy gap of Brønsted acid-catalyzed cellulose conversion were significantly reduced by adding these solvents. Molecular dynamics (MD) simulation revealed that in 1,4-dioxane/H 2 O system, H 2 SO 4 was aggregated to the ring oxygen of cellobiose, as well as the C1-OH and C2-OH groups of glucose. This aggregation process effectively promoted the protonation of cellobiose at the O1 position. The reaction conditions were optimized via response surface methodology leading to a maximum HMF yield of 56.5 % in 1,4-dioxane/H 2 O (water content 5.3 %),under the conditions of 193 °C and 67 min. The synergistic mechanism will be beneficial for the rational selection of system in the production of high-valued chemicals from biomass.
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CytoJournal
·
2025
·
IF 3.1
Xunjia Li,Zhixin Xu,Ying Li,Yan Luo,Jie Zhou,Deyu Zuo,Weijian Xiong
Objective:
Patients with chronic kidney disease (CKD) exhibit increased vascular calcification (VC) risks, worsened by high-dose erythropoietin (EPO). While EPO treats anemia, its role in VC pathogenesis remains unclear. Ginsenoside Rb1 (Rb1), a Panax ginseng compound with anti-calcification properties, may counteract EPO-induced VC through the GATA binding protein 6 (GATA6)/bone morphogenetic protein 2 (BMP2)/Smad1/5/9 pathway. This article aims to explore whether Rb1 could counteract EPO-induced VC through the GATA6/BMP2/Smad1/5/9 pathway.
Material and Methods:
Adenine-induced CKD rats and b-glycerophosphate-treated vascular smooth muscle cells (VSMCs) received EPO ± Rb1. Calcification was assessed through von Kossa/alizarin red staining. Smooth muscle protein 22-a (SM22a)/a-Smooth muscle actin (a-SMA) expression was measured by immunofluorescence and real-time-quantitative polymerase chain reaction (RT-qPCR). GATA6/BMP2/Smad1/5/9 activation was analyzed using RT-qPCR/Western blot. Rb1-BMP2 interactions were tested through biotin pulldown, micro-thermophoresis, and Co-immunoprecipitation (Co-IP). GATA6 knockdown validated pathway roles.
Results:
High-dose EPO significantly worsened CKD-associated calcification and VSMC calcification (P < 0.01), suppressed SM22a and a-SMA expression levels, and activated the GATA6/BMP2/Smad1/5/9 pathway (P < 0.01). GATA6 knockdown reduced EPO-exacerbated calcification and modulated BMP2/Smad1/5/9 signaling (P < 0.01). Rb1 increased SM22a and a-SMA expression levels and inhibited Smad 1/5/9 phosphorylation (P < 0.01), without affecting GATA6 or BMP2 expression (P > 0.05). Molecular docking and Co-IP experiments revealed that Rb1 binds directly to BMP2, blocking its interaction with bone morphogenetic protein receptor and inhibiting Smad 1/5/9 phosphorylation (P < 0.01).
Conclusion:
Rb1 mitigates EPO-aggravated VC in CKD by disrupting BMP2/Smad1/5/9 signaling, positioning it as a promising molecular intervention strategy to reduce EPO-induced vascular toxicity.
Keywords: Bone morphogenetic protein 2/Smad1/5/9 signaling pathway, Chronic kidney disease, Erythropoietin, Ginsenoside Rb1, Vascular calcification
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JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 38
·期 14
·2387-2393
·
IF 2.737
Jun Yan,Qi-Lei Zhang,Hong-Fei Tong,Dong-Qiang Lin,Shan-Jing Yao
A new hydrophobic charge-induction chromatography resin was prepared with 5-aminobenzimidazol as functional ligand and polyacrylic ester beads as matrix. Adsorption isotherms and adsorption in columns were investigated using human immunoglobulin G and bovine serum albumin as model proteins, and the influence of pH and NaCl concentration was discussed. Results showed that the ligand density was 195 μmol/mL gel, and protein selectivity can be improved by controlling pH and salt addition. An optimized purification process (sample loading at pH 8.0 with 0.2 M NaCl and elution at pH 5.0) was performed to purify human immunoglobulin G from bovine serum albumin containing feedstock, which resulted in human immunoglobulin G purity of 99.7% and recovery of 94.6%. A similar process was applied for the purification of monoclonal antibody from cell culture supernatant, which showed antibody purity of 94.9% and recovery of 92.5%. The results indicated that the new resin developed had comparable performance as Protein A chromatography and would be suitable for antibody purification from complex feedstock.
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JOURNAL OF SOLID STATE CHEMISTRY
·
2024
·
IF 3.2
Qian Zhang,Jun Li,Tian-Jiao Yue,Na Li,Yu-Qin Jiang,Hai-Ming Guo
Four hyper-cross-linked polymers were synthesized by linking electron-rich monomers with an electron-rich cross-linker via the Friedel–Crafts reaction. Among these polymers, P4 , which was constructed with decaalkyne pillar[5]arene and bibenzyl chloride, adsorbed iodine vapor with a capacity of 3.930 g g −1 , outperforming most supramolecular macrocycle-based porous polymers . The pillar[5]arene macrocycle contributed various types of adsorption sites, including aromatic rings , diynes, and oxygen atoms. The abundance of effective adsorption sites and the high specific surface area (1615.5 m 2 g −1 ) were responsible for the excellent adsorption capacity of P4 . Because these polymers are easy to synthesize and regenerate and can be mass produced, they are promising candidates for large-scale applications.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2025
·
IF 4.7
Zhan Liu,Jia-Min Lyu,Chun-Mu Guo,Zhi-Yi Hu,Ming-Hui Sun,Li-Hua Chen,Bao-Lian Su
Hierarchically porous zeolites, combining the intrinsic microporous catalytic properties and the enhanced meso-/macroporous access and transport, exhibit excellent performances in many highly efficient catalytic processes. The acidity and porosity are two key factors affecting the catalytic conversion, selectivity and lifetime. It is necessary to construct such hierarchically porous zeolite catalysts with adjustable acid active sites and pore properties in a wide range to meet the specific catalytic requirements. Herein, an ordered interconnected hierarchical ZSM-5 single crystal catalyst composed by zeolite sphere units stacking in face-centered cubic (FCC) arrangement has been used as the theoretical model to research the corresponding material properties on the enhancement of specific catalytic process. Specifically, the properties of acid sites and meso-/macropores can be accurately adjusted by facilely changing the SiO 2 /Al 2 O 3 molar ratio (100–1000) and the sphere unit size (180–580 nm), respectively. As a result, the benzyl alcohol liquid-phase catalytic ability markedly improved thanks to the optimized acidity and meso-/macropore size. The larger meso-/macropore size shows higher benzyl alcohol conversion, while the higher SiO 2 /Al 2 O 3 molar ratio exhibits higher alkylation product selectivity. This work illustrate that the targeted catalytic process can be effectively enhanced by adjusting the acidity and porosity within such ordered hierarchical ZSM-5 single crystal system.
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NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Xiude Peng,Xiaohua Cao,Huaiyu Xu,Ruibin Xiong,Dedong He,Dingkai Chen,Yongming Luo
CO2-ODHP has been demonstrated to be an effective technology for the production of propylene. CO2 acts as a soft oxidizer that reduces over-oxidation of hydrocarbons and improves propylene selectivity. However, CO2-ODHP still faces the challenge of rapid catalyst deactivation due to coke accumulation at elevated operating temperatures. Herein, we prepared a La-modified Ga/ZSM-5 catalyst, in which La modified the acidic–basic properties of Ga/ZSM-5 and improved the resistance to coke accumulation of the catalyst. The synthesized Ga2La1/ZSM-5 catalyst achieved propane conversion up to 80% at 580 °C. In situ DRIFTS analysis revealed that the addition of La could significantly accelerate the formation of HCOO* intermediates, which further improved the efficiency of the CO2-ODHP reaction.
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Arabian Journal of Chemistry
·
2022
·
IF 6.212
Zizhen Zhao,Chen Fu,Yuping Zhang,Yingying Zhang,Xiaoxi Yang,Ailing Fu
The incidence of HCC continues to increase rapidly in recent years. the principle of developing anti-HCC drugs is to specifically kill tumor cells, without affecting the function of normal cells. Here we synthesized a novel protonic compound bis(5-amino-1,10-phenanthroline) (P-BAP), and the antitumor activity was explored in vitro and in vivo . The results showed that P-BAP could selectively inhibit the proliferation of tumor cells, and induce HCC apoptosis. In HCC-bearing mice, P-BAP could effectively retard the tumor growth, even completely eliminate the tumors at the dose of 5 mg/kg, and meanwhile P-BAP had no significant effect on mouse body weight. Mechanism analysis revealed that P-BAP could down-regulate the protein expressions of pleomorphic adenoma gene like-2 (PLAGL2), HIF-1α and β-catenin, and up-regulate the levels of pro-apoptotic protein BAX and BNIP3. In addition, P-BAP could reduce mitochondrial respiratory chain complex activities, leading to insufficient ATP production. The study provides a new approach for designing selective antitumor drugs, and suggests that P-BAP would be a potential candidate for HCC therapy.
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Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Yang Bin,Sun Yiwen,Fu Shouying,Xia Miaomiao,Su Yuan,Liu Chuan,Zhang Chunzhi,Zhang Dawei
Ribulose 5-phosphate (Ru5P) and guanosine 5′-triphosphate (GTP) are two key precursors of riboflavin, whereby Ru5P is also a precursor of GTP. Ribulose 5-phosphate 3-epimerase (Rpe) catalyzes the conversion of ribulose 5-phosphate into xylulose 5-phosphate. Inactivation of Rpe can reduce the consumption of Ru5P, enhancing the carbon flux toward riboflavin biosynthesis. Here we investigated the effect of mutation of rpe and other related genes on riboflavin production, physiological and metabolic phenotypes in Bacillus subtilis LY (BSLY). Introducing single nucleotide deletion (generated BSR) or nonsense mutation (generated BSRN) on the genomic copy of rpe, resulting in more than fivefold increase of riboflavin production over the parental strain. BSR process 62% Rpe activity, while BSRN lost the entire Rpe activity and had a growth defect compared with the parent strain. BSR and BSRN exhibited increases of the inosine and guanine titers, in addition, BSRN exhibited an increase of inosine 5′-monophosphate titer in fermentation. The transcription levels of most oxidative pentose phosphate pathway and purine synthesis genes were unchanged in BSR, except for the levels of zwf and ndk, which were higher than in BSLY. The production of riboflavin was increased to 479.90 ± 33.21 mg/L when ribA was overexpressed in BSR. The overexpression of zwf, gntZ, prs, and purF also enhanced the riboflavin production. Finally, overexpression of the rib operon by the pMX45 plasmid and mutant gnd by pHP03 plasmid in BSR led to a 3.05-fold increase of the riboflavin production (977.29 ± 63.44 mg/L), showing the potential for further engineering of this strain.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yan Sun,Ranxin Li,Qiubo Wang,Wen-da Oh,Mingjie Huang,Xiaohui Wu,Tao Zhou
The concept of converting biowaste to energy is popular worldwide; however, overcoming the trade-off between bio-oil recovery and obtaining functional biochar remains challenging. This study investigated the effects of three transition metal ions (Mn 2+ , Cu 2+ and Fe 2+ ) throughout the entire process of recovering 5-hydroxymethylfurfural (5-HMF) from forestry waste and on the catalytic abilities of biochar derived from the oil-extracted residue. The Cu 2+ , Fe 2+ , and Mn 2+ ions significantly increased 5-HMF yields by 93- to 158-fold compared to the metal-free control. However, only marginal improvements in the catalytic ability of the residue-derived biochar for persulfate activation were observed for Cu- or Fe-doped biochar, despite the general benefit of transition metal doping for biochar functionalization. Interestingly, a dual Mn-mediated promotion was achieved beyond enhancing 5-HMF production. The Mn-doped biochar (Mn-Biochar) exhibited excellent peroxydisulfate (PDS) activation capability, enhancing the degradation of the antibiotic sulfamethoxazole (SMX) by 6.6- to 15.0-fold. Characterizations revealed that incorporated nitrogen was responsible for this enhancement. N-doping transformed the Mn species into MnO, which tripled the Mn loading capacity and enhanced the electrical conductivity of the biochar. The formed Mn-N x structure in the biochar (Mn-N-FBBC) facilitated polarization of the O O bond and cleavage of the S O bond in PDS, generating 1 O 2 for SMX degradation. Life cycle assessment (LCA) indicated that this dual Mn-mediated process for 5-HMF recovery and biochar production offers synergistic benefits, realizing the “waste-resource-material-treatment” paradigm in biomass conversion.
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Oncology Letters
·
2021
·Vol. 23
·期 2
·1-10
·
IF 2.967
Zheng Han,Liu Meng,Xiaodong Huang,Jie Tan,Weijie Liu,Wei Chen,Yanli Zou,Yishan Cai,Shasha Huang,Aifang Chen,Ting Zhan,Min Huang,Xiaoli Chen,Xia Tian,Qingxi Zhu
A major cause of treatment failure in advanced colon cancer is resistance to chemotherapy. p38 mitogen‑activated protein kinase (MAPK) has been associated with cellular apoptosis and plays an important role in multidrug resistance (MDR) in cancer cells. In the present study the effect of p38 MAPK on the sensitivity of 5‑fluorouracil (5‑FU)‑resistant SW480 (SW480/5‑FU) human colon cancer cells to noscapine was investigated. Following p38 MAPK interference, the inhibitory effect of noscapine on cell viability and proliferation was increased in the SW480/5‑FU cells and there was also a decrease in the expression level of minichromosome maintenance proteins, recombinant Ki‑67 and proliferating cell nuclear antigen. Inhibition of p38 MAPK also enhanced noscapine‑induced G1‑phase cell cycle arrest in the SW480/5‑FU cells and there was also a decrease in the protein and mRNA expression level of cyclin D, cyclin E and cyclin‑dependent kinase 2, and an increase in the expression level of P57. Furthermore, p38 MAPK interference increased noscapine‑induced apoptosis of the SW480/5‑FU cells and there was an increase in the protein and mRNA expression level of caspases‑3 and 8 and Bax, and decreased Bcl‑2 expression level. The sensitivity of the SW480/5‑FU cells to noscapine was also increased following p38 MAPK interference, as demonstrated by MDR inhibition via decreased Akt activity and reduced protein expression level of the MDR proteins P‑glycoprotein, multidrug resistance protein 1 and ATP‑binding cassette G2. These observations indicated that inhibition of p38 MAPK increased the sensitivity of the SW480/5‑FU cells to noscapine by suppressing proliferation, induction of cell cycle arrest and apoptosis, and reversal of MDR in the SW480/5‑FU cells.
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