Biomaterials Advances
·
2023
·
IF 0
Jie Wang,Yiming Sun,Xiaoqing Liu,Yongyuan Kang,Wangbei Cao,Juan Ye,Changyou Gao
Both bacteria-infection and excessive inflammation delay the wound healing process and even create non-healing wound, thus it is highly desirable to endow the wound dressing with bactericidal and anti-oxidation properties. Herein an antibacterial and antioxidation hydrogel based on Carbomer 940 (CBM) and hydroxypropyl methyl cellulose (HPMC) loaded with tea polyphenols (TP) and hyperbranched poly- l -lysine (HBPL) was designed and fabricated. The hydrogel killed 99.9 % of methicillin-resistant Staphylococcus aureus ( MRSA ) and Escherichia coli ( E. coli ) at 10 7 CFU mL −1 , and showed strong antioxidation against H 2 O 2 and 2,2-di(4- tert -octylphenyl)-1-picryl-hydrazyl (DPPH) radicals without noticeable cytotoxicity in vitro . The CBM/HPMC/HBPL/TP hydrogel significantly shortened the inflammatory period of the MRSA-infected full-thickness skin wound of rats in vivo , with 2 orders of lower MRSA colonies compared with the blank control, and promoted the wound closure especially at the earlier stage. The inflammation was suppressed and the vascularization was promoted significantly as well, resulting in reduced pro-inflammatory factors including interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), and increased anti-inflammatory factors such as interleukin-4 (IL-4) and interleukin-10 (IL-10).
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PubMed
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 40
·19056-19064
·
IF 3.591
Xiaochun Deng,Jingwen Zhao,Yao Ding,Hongliang Tang,Fengna Xi
As an alternative to natural enzymes, peroxidase nanozymes have shown great potential in the fields of sensing, drug delivery, and tumor treatment. However, obtaining peroxidase nanozymes with high catalytic activity, simple synthesis, and low cost is still a huge challenge. Herein, we demonstrate facile synthesis of iron and nitrogen co-doped graphene quantum dots (Fe,N-GQDs), which possess high peroxidase activity. With hemin being a precursor, Fe,N-GQDs can be easily synthesized with a high production yield (74%) using a one-step hydrothermal process. The as-prepared Fe,N-GQDs exhibit uniform size (2.9 nm), good water dispersibility and bright yellow-green fluorescence (an absolute fluorescence quantum yield of 12.5%). In particular, Fe,N-GQDs exhibit very high peroxidase-mimicking activity and can rapidly oxidize peroxidase substrates 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue oxidized TMB (oxTMB) in the presence of H2O2. In comparison with a natural enzyme horseradish peroxidase (HRP), the Fe,N-GQD nanozyme has a lower Michaelis constant (Km) and a higher maximum reaction rate (Vmax), indicating higher substrate affinity and faster reaction rate. Selective and sensitive colorimetric detection of L-cysteine (L-Cys) is realized in terms of its ability to reduce oxTMB and to react with H2O2. The linear range for the detection is 0.5–50 μM with a limit of detection (LOD) of 140 nM. In addition, the detection of L-Cys in compound amino acid tablets is also realized.
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高影响因子
LUMINESCENCE
·
2021
·Vol. 36
·期 6
·1513-1524
·
IF 2.464
Huan Zhang,Zhenzhen Li,Huaying Huang,Si Ouyang,Yiqing Deng,Qiang Zhao
Near-infrared (NIR) core–shell CdSeTe/CdS quantum dots (QDs) modified with l -cysteine were synthesized in aqueous solution. The QDs had a special NIR-emitting spectrum, high fluorescence stability and low cytotoxicity. In addition, they exhibited an obvious fluorescence quenching when Cu 2+ was present. An NIR nanosensor was prepared for rapidly, sensitively, and selectively determining Cu 2+ in solution quantitatively and monitoring the changes in Cu 2+ in cells with fluorescence imaging in a semiquantitative way. The linear relationship between the relative fluorescence intensity (F 0 /F) and the concentration of Cu 2+ from 5.12 × 10 −8 M to 2.56 × 10 −5 M in solution was observed using an NIR fluorescence spectrophotometer with R 2 equal to 0.9958. Moreover, in the experiment with the fluorescence microscope, F 0 /F versus the concentration of Cu 2+ from 5.00 × 10 −8 M to 7.68 × 10 −6 M also showed a good linear relationship with R 2 equal to 0.9817. Practical water sample ion detecting experiments had good accuracy and recovery rates. Cell experiments showed that the NIR imaging intensity of cells was inversely proportional to the concentration of copper ions, therefore NIR QDs have great potential for detection of metal ions in solution and in cells.
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PubMed
高影响因子
LANGMUIR
·
2021
·
IF 3.882
Lingqi Zhong,Haizheng Tao,Xiao Gong
In this work, superhydrophobic poly(l-lactic acid) (PLLA) hierarchical membranes exhibiting excellent oil-removal performance, which is of great importance in curbing the oil-pollution environment, were fabricated by a simple solvent-evaporation-induced precipitation method. PLLA membranes with hierarchical micro/nanostructures (fish scales, fibrous sheets, and petal-like morphology) can be conveniently prepared by adjusting the preparation parameters including PLLA concentration, precipitation temperature, type of solvent and nonsolvent, and the addition of nano-SiO2. The results show that the water contact angle of the fish-scale-structured PLLA membrane was 138.6°, revealing that water repellency was significantly improved compared to that of the solvent-casting PLLA membrane (∼72.8°). Moreover, the PLLA/SiO2 nanocomposite membrane with a dense hierarchical micro/nanostructure had a water contact angle greater than 167.1°, which has great potential in oil–water separation.
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高影响因子
JOURNAL OF BIOLOGICAL CHEMISTRY
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2021
·Vol. 297
·期 1
·
IF 5.157
Bochuan Yuan,Ziwei Wu,Wei Ji,Dong Liu,Xiang Guo,Donghui Yang,Aili Fan,Hongli Jia,Ming Ma,Wenhan Lin
The fungal cyclohexadepsipeptides destruxins (DTXs), isaridins (ISDs), and isariins (ISRs) are nonribosomal peptides whose structures include a 19-membered ring composed of five amino acid residues and one α- or β-hydroxy acid residue. These cyclohexadepsipeptides contain unusual nonproteinogenic amino acid–building blocks and possess a range of antiviral, antibacterial, and other activities. The biosynthetic gene clusters for ISDs and ISRs have not been identified, and the biosynthesis of the nonproteinogenic (3S)-methyl-l-proline residue, which is found in DTXs, ISDs, and many other natural products, lacks full characterization. In an ongoing effort to identify compounds that can inhibit the Zika virus (ZIKV), we examined the extract of marine-derived fungus Beauveria felina SX-6-22 and discovered 30 DTXs, ISDs, and ISRs (1–30) including seven new compounds (1–7). The anti-ZIKV assays showed that 9–12 and 16–18 possess inhibitory activities against ZIKV RNA replication and NS5 (nonstructural protein 5) production in ZIKV-infected A549 cells. We sequenced the genome of B. felina SX-6-22 and identified three biosynthetic gene clusters detx, isd and isr, which are responsible for the biosynthesis of DTXs, ISDs, and ISRs, respectively. Comparative analyses of the three gene clusters clarified the biosynthetic relationships among these cyclohexadepsipeptides. Finally, we characterized the entire biosynthesis of nonproteinogenic building block (3S)-methyl-l-proline. The Δ1-pyrroline-5-carboxylate reductases (P5CRs), also used in the biosynthesis of l-proline, were demonstrated to catalyze the final reduction step in (3S)-methyl-l-proline formation, suggesting potential cross talk between primary and secondary metabolisms. These results provide opportunities for biosynthetic pathway engineering to generate new anti-ZIKV cyclohexadepsipeptides.
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高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 32
·3561-3568
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IF 2.896
Lihong Shi,Xiaorui Dong,Guomei Zhang,Yan Zhang,Caihong Zhang,Chuan Dong,Shaomin Shuang
N-doped blue-fluorescence carbon dots (N-CDs) were fabricated via a one-pot hydrothermal method using folic acid and p-phenylenediamine. The obtained N-CDs exhibited strong fluorescence (FL) with a considerable quantum yield (QY) of 21.8% and exceptional optical stability under different conditions. Upon introducing Cr(VI), blue FL of N-CDs was distinctly quenched. On subsequent addition of L-AA, the FL of N-CDs could be partially recovered. The fluorescence changes of N-CDs have been utilized to detect Cr(VI) and L-AA in aqueous solutions with linear ranges of 0.10–150 μM and 0.75–2.25 mM, respectively, as well as limit of detection values of 9.4 nM and 25 μM, respectively. Furthermore, as-obtained N-CDs can be extended to monitor the fluctuation of intracellular Cr(VI) and L-AA. More intriguingly, N-CDs can target lysosomes with a satisfactory Pearson correction coefficient of 0.87, which indicates a promising application prospect in the biomedical field.
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Xueying Zong,Juanjuan Zhang,Jinwei Zhu,Linyu Zhang,Lijuan Jiang,Ying Yin,Hongyan Guo
Microplastics are widespread in freshwater environments, their biological effects and combined effects of other pollutants have attracted extensive attention. In this study, we investigated the adsorption properties of heavy metals onto polystyrene (PS) microplastics as well as the bioavailability and toxicity of microplastics and heavy metals by hydroponic wheat seedlings experiment. Results showed that PS microplastics (0.5 µm, 100 mg/L) had no significant effect on wheat seedlings growth, photosynthesis, and reactive oxygen species (ROS) content. However, PS microplastics could adsorb copper and cadmium, with a predominantly chemisorption. The accumulation of copper and cadmium in wheat seedlings reduced in the presence of PS microplastics, which meant the toxic effect by heavy metals might be mitigated. Compared with single heavy metals treatments, the combination of PS microplastics and heavy metals increased chlorophyll content, enhanced photosynthesis and reduced the accumulation of ROS. These findings suggest that PS microplastics (0.5 µm, 100 mg/L) have a mitigating effect on the bioavailability and toxicity of copper and cadmium.
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高影响因子
Natural Product Communications
·
2020
·
IF 0.468
YanHua Tian,HuiZhi Du,Li Wang,ShiFei Li,Lu Zhang,LiWei Zhang
Diospyros lotus L. leaves are used as a functional tea and folk medicine in several Asian countries. This study aimed to evaluate the effects of phenolic extracts of the leaves on scavenging nitrite and inhibiting N-nitrosamines (NAs) formation and to determine the active ingredients responsible for these effects. Of the 7 fractions (Fr1-Fr7) prepared from the extract of D. lotus leaves, Fr5 contained the highest phenolic content and exhibited the strongest activity. Five active ingredients of Fr5 were discovered, and 4 of them were identified as myricitrin (Mytr), myricetin (Myt), myricetin-3-O-β-d-glucoside (Myt-Glc), and myricetin-3-O-β-d-galactoside (Myt-Gal). The content of Mytr was much higher than those of the other 3 ingredients, both in Fr5 and extracts of D. lotus leaves. Finally, Mytr and Myt were proved to have stronger activities by the 1,1-diphenyl-2-trinitrophenyl hydrazine scavenging, nitrite scavenging, and inhibition of NAs formation assays. These results indicated that Mytr was the main active ingredient of D. lotus leaves. Myt, Mytr, and Fr5 from the leaves could be used as natural agents for antioxidant, nitrite scavenging, and inhibition of NAs formation in food and the human body.
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高影响因子
POLYMER DEGRADATION AND STABILITY
·
2020
·
IF 4.032
Qianyu Sun,Jie Sheng,Rendang Yang
Controllable degradation is important for the biodegradable polymers used as drug carriers, because the drug release rate can be regulated by the degradation rate. Here, we synthesized chitosan-graft-poly(D, L-lactic acid) copolymers (CgPCs) with high MS PDLLA (molar substitution of poly(D, L-lactic acid)), which possessed controllable biodegradability for regulating the drug release rate. CgPCs with high MS PDLLA were prepared efficiently through an eco-friendly system of 1-ethy-3-methyimidazolium acetate (EmimAc)/4-Dimethylaminopyridine (DMAP). The MS PDLLA reached the maximum of 12.00 within 5 hours, which involved 2.91 of DS PDLLA (the degree of substitution of poly(D, L-lactic acid)) and 4.13 of DP PDLLA (the degree of polymerization of poly(D, L-lactic acid)). Importantly, the biodegradation rate of CgPCs could be regulated by the MS PDLLA . CgPCs with MS PDLLA of 3.98 showed good hydrophilicity and fast degradation leading to burst release of curcumin, whereas CgPCs with MS PDLLA of 12.00 exhibited relatively poor hydrophilicity and slower degradation resulting in sustained release of curcumin. This study provides some new possibilities for controllable biodegradation of drug carriers and optionality of burst or sustained drug release.
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高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2020
·
IF 7.727
Zhihao Guo,Junhui Sui,Mengcheng Ma,Jianshe Hu,Yong Sun,Liqun Yang,Yujiang Fan,Xingdong Zhang
Stimuli-responsive nanotechnology-mediated drug co-delivery system is a notable strategy to improve access of the systemically administered chemotherapeutics to the tumors. Herein, a tailor-made 2,3-dimethylmaleic-anhydride-poly(ethylene glycol)- ε -poly- l -lysine-doxorubicin /lapatinib polymeric nanoplatform (DMMA-P-DOX/LAP) for synergistically eliminating breast cancer is developed by encapsulating lapatinib into dual-pH responsive charge switchable biopolymer-doxorubicin conjugate nanoparticles . The physicochemical properties of polymeric nanoparticles are conducive to their stable circulation in the physiological condition, but reverse the surface charge from negative to positive ultrasensitively in slightly acidic tumor microenvironment , facilitating cell internalization and deep tumor penetration. Subsequently, DOX and LAP are synchronously released into the cytoplasm in response to the significantly increased acidity of intracellular environment. As a result, the combination therapy by DMMA-P-DOX/LAP nanoparticles compels the solid tumors to contract significantly or even vanish completely in the MCF-7 tumor model, moreover, the structural composition with amino acid and bioinert PEG ensures the favorable biosecurity of the co-delivery system in vivo . This dual-pH responsive nanotechnology-mediated drug co-delivery system provides great potentials for safe and effective cancer therapy.
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高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2020
·
IF 4.96
Cui Li,Liping Li,Yaodong Yi,Wei Wang,Jingqun Yuan,Fuqing Tan,Danbo Fang,Su Zeng,Hui Zhou,Huidi Jiang
Cisplatin is a first-line chemotherapeutic agent that is widely used for treatment of various solid tumors. However, cisplatin-induced adverse effects, particularly severe nephrotoxicity, preclude its application. In this study, we showed that L-tetrahydropalmatine ( L-THP) could selectively inhibit organic cation transporter 2 (OCT2), which plays a crucial role in renal cisplatin uptake from the circulation. Additionally, we demonstrated that L-THP attenuated cisplatin-induced toxicity in mouse primary renal tubular cells. Subsequently, we verified that L-THP reduced the renal accumulation of cisplatin and alleviated cisplatin-induced renal injury in healthy and tumor-bearing nude mice. In healthy mice, co-treatment of L-THP at 5–40 mg/kg reduced cisplatin renal accumulation to 75.0%-49.9% of that in cisplatin alone group (10 mg/kg), and alleviated cisplatin-induced nephrotoxicity. Additionally, it did not alter Pt concentration in the tumor tissue and did not impair its antitumor efficacy in tumor bearing nude mice. The tumor inhibitory rates of cisplatin (10 mg/kg) co-treated with L-THP at 10, 20 and 40 mg/kg were 71.4%, 70.4% and 69.4%, respectively, in H460 tumor bearing nude mice, higher than that of in cisplatin alone group (60.6%), while in HCT116 tumor bearing nude mice, the tumor inhibitory rates in co-treated with 20 mg/kg L-THP was 34.7% ( vs 26.3% in cisplatin alone group). Moreover, L-THP reduced cisplatin accumulation and alleviated cisplatin-induced cytotoxicity in human primary renal tubular cells. Therefore, our findings suggested that concomitant administration of L-THP could attenuate cisplatin-induced renal injury via selective inhibition of OCT2 without impairing its antitumor efficacy.
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高影响因子
Advanced Fiber Materials
·
2020
·Vol. 2
·期 2
·105-117
·
IF 0
Yang Xiaoping,Li Lingfeng,Yang Dongzhi,Nie Jun,Ma Guiping
Biomimetic scaffolds made by synthetic materials are usually used to replace the natural tissues aimed at speeding up the skin regeneration. In this study, a flexible and cytocompatible poly(glycerol sebacate)@poly- l -lactic acid (PGS@PLLA) fibrous scaffold with a core–shell structure was fabricated by coaxial electrospinning, where the shell PLLA was used to be a skeleton with pores on the fibrous surface. The fibrous morphology with pores on the surface of the prepared fibers was observed by SEM. The core–shell microstructure of PGS@PLLA fibers was confirmed by TEM and Laser Scanning Confocal Microscopy (LSCM). In addition, the prepared fibers exhibited a strong ability to repair tissues of the skin wound, where the stability of cell security and proliferation, and the lower inflammatory response were all superior to those of pure PLLA scaffold. It’s worth noting that the percentage of skin tissue was regenerated by 95% within 14 days, which suggests the potential application for electrospun-based synthetic fibrous scaffolds on wound healing.
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危险品化学品经营许可证（带存储）