APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2024
·
IF 3.1
Qiao Xi,Jiang Mengna,Zhu Enze,Gu Yiwen,Chen Zhuoran,Ju Xin,Li Liangzhi,Zhong Xia,Chen Zhi
Chitin, a natural organic compound with content slightly lower than cellulose, is also known for chitosan, a substance derived from chitin through deacetylation. In this experiment, preliminary screening was conducted using the plate discoloration circle method, leading to the selection of a high-yield CDA-producing strain from 28 candidates through rescreening. Morphological characteristics and 16S rDNA sequence analysis revealed 99.93% homology with Enterobacter sichuanensis strain N24, thus naming this strain Enterobacter strain ZCDA27. Initial fermentation of the strain yielded CDA activity of 9.29 U/mL. Single-factor optimization was then performed, followed by a PB test to screen for significant factors affecting enzyme production. The response surface method was used to further optimize the fermentation conditions. The optimal fermentation conditions for the carbon source, nitrogen source, metal ion, fermentation temperature, time, liquid volume, and initial pH were explored. Significant factors affecting enzyme production, including MgSO 4 , initial medium pH, and fructose levels, were identified using the PB test. Finally, the fermentation conditions of ZCDA27 were optimized using the Box-Behnken design combined with RSM, which comprised fructose at 1.020%, magnesium sulfate at 0.016%, and peptone at 1%. The fermentation conditions included a temperature of 37, initial pH of 7.1, rotation speed of 140 × g , fermentation time of 28 h, inoculation amount of 2%, and liquid volume of 40%. Under these conditions, the enzyme activity of ZCDA27 reached 14.52 U/mL, a 1.6-fold increase from the pre-optimization levels. In summary, this study provides an experimental foundation for further development and application of Enterobacter spp. ZCDA27 CDA.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·
IF 12.6
Xinyao Hu,Hua Zhu,Yang Shen,Lang Rao,Jiayi Li,Xiaoqin He,Ximing Xu
Tumor immunotherapy aims to harness the immune system to identify and eliminate cancer cells. However, its full potential is hindered by the immunosuppressive nature of tumors. Radiotherapy remains a key treatment modality for local tumor control and immunomodulation within the tumor microenvironment. Yet, the efficacy of radiotherapy is often limited by tumor radiosensitivity, and traditional radiosensitizers have shown limited effectiveness in hepatocellular carcinoma (HCC). To address these challenges, we developed a novel multifunctional nanoparticle system, ZIF-8@MnCO@DOX (ZMD), designed to enhance drug delivery to tumor tissues. In the tumor microenvironment, Zn²⁺ and Mn²⁺ ions released from ZMD participate in a Fenton-like reaction, generating reactive oxygen species (ROS) that promote tumor cell death and improve radiosensitivity. Additionally, the release of doxorubicin (DOX)-an anthracycline chemotherapeutic agent-induces DNA damage and apoptosis in cancer cells. The combined action of metal ions and double-stranded DNA (dsDNA) from damaged tumor cells synergistically activates the cyclic GMP-AMP synthase (cGAS)–stimulator of interferon genes (STING) pathway, thereby initiating a robust anti-tumor immune response. Both in vitro and in vivo experiments demonstrated that ZMD effectively activates the cGAS-STING pathway, promotes anti-tumor immune responses, and exerts a potent tumor-killing effect in combination with radiotherapy, leading to regression of both primary tumors and distant metastases. Our work provides a straightforward, safe, and effective strategy for combining immunotherapy with radiotherapy to treat advanced cancer.
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Microbial Cell Factories
·
2024
·Vol. 23
·期 1
·1-12
·
IF 4.3
Wang Jiajia,Chen Yumeng,Cong Jiawei,Wang Wei
Background Trichoderma reesei is renowned for its cellulase-producing ability and is used for biofuel production from lignocellulose. In plants and fungi, cellulase production is induced by cellulose and suppressed by glucose; however, whether metformin can enhance cellulase production and mitochondrial function in T. reesei remains unclear. Metformin reduces blood glucose levels by inhibiting hepatic gluconeogenesis; therefore, it is worth investigating whether metformin transmission modulates cellulase biosynthesis in T. reesei . Results Metformin increased cellulase production and the transcription of cellulase-related genes. It also enhanced the concentrations of Ca 2+ in the cytosol and mitochondria and regulated the transcription levels of cellulase-related genes by modulating calcium homeostasis in T. reesei QM6a. In addition, metformin was identified as an antioxidant that can enhance cellulase activity by reducing reactive oxygen species (ROS). Our results demonstrated that metformin influences the state of mitochondria by enhancing mitochondrial activity and membrane potential to promote cellulase production. Conclusion Collectively, these results indicate that metformin enhances cellulase production, scavenges ROS, and protects mitochondrial activity in T. reesei . Graphical Abstract
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-11
·
IF 5.3
Wang Na,Li Huiru,Tian Yanbo,Tan Liju,Cheng Shuzhen,Wang Jiangtao
17β-Estradiol (E2) is the typical endocrine disruptor of steroidal estrogens and is widely used in animal husbandry and dairy processing. In the environment, even lower concentrations of E2 can cause endocrine dysfunction in organisms. Herein, we have developed a novel molecularly imprinted ratiometric fluorescent sensor based on SiO 2 -coated CdTe quantum dots (CdTe@SiO 2 ) and 7-hydroxycoumarin with a post-imprint mixing strategy. The sensor selectively detected E2 in aqueous environments due to its two fluorescent signals with a self-correction function. The sensor has been successfully used for spiking a wide range of real water and milk samples. The results showed that the sensor exhibited good linearity over the concentration range 0.011–50 μg/L, obtaining satisfactory recoveries of 92.4–110.6% with precisions (RSD) < 2.5%. Moreover, this sensor obtained an ultra-low detection limit of 3.3 ng/L and a higher imprinting factor of 13.66. By using estriol (E3), as a supporting model, it was confirmed that a simple and economical ratiometric fluorescent construction strategy was provided for other hydrophobic substances. Graphical Abstract
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Journal of Bionic Engineering
·
2025
·
IF 5.8
Yu Zerun,Liu Jiaan,Yang Tian,Zhang Linyang,Hu Chunhua
Insufficient interfacial activity and poor wettability between fibers and matrix are the two main factors limiting the improvement of mechanical properties of Carbon Fiber Reinforced Plastics (CFRP). Owl feathers are known for their unique compact structure; they are not only lightweight but also strong. In this study, an in-depth look at owl feathers was made and it found that owl feathers not only have the macro branches structure between feather shafts and branches but also have fine feather structures on the branches. The presence of these fine feather structures increases the specific surface area of the plume branches and allows neighboring plume branches to hook up with each other, forming an effective mechanical interlocking structure. These structures bring owl feathers excellent mechanical properties. Inspired by the natural structure of owl feathers, a weaving technique and a sizing process were combined to prepare bionic Carbon Fiber (CF) fabrics and then to fabricate the bionic CFRP with structural characteristics similar to owl feathers. To evaluate the effect of the fine feather structure on the mechanical properties of CFRP, a mechanical property study on CFRP with and without the fine feather imitation structure were conducted. The experimental results show that the introduction of the fine feather branch structure enhance the mechanical properties of CFRP significantly. Specifically, the tensile strength of the composites increased by 6.42% and 13.06% and the flexural strength increased by 8.02% and 16.87% in the 0° and 90° sample directions, respectively. These results provide a new design idea for the improvement of the mechanical properties of the CFRP, promoting the application of CFRP in engineering fields, such as automotive transportation, rail transit, aerospace, and construction.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-18
·
IF 10.6
Yin Mingjing,Lei Doudou,Liu Yalan,Qin Tao,Gao Huyang,Lv Wenquan,Liu Qianyue,Qin Lian,Jin Weiqian,Chen Yin,Liang Hao,Wang Bailei,Gao Ming,Zhang Jianfeng,Lu Junyu
Acute lung injury (ALI) is a life threatening disease in critically ill patients, and characterized by excessive reactive oxygen species (ROS) and inflammatory factors levels in the lung. Multiple evidences suggest that nanozyme with diversified catalytic capabilities plays a vital role in this fatal lung injury. At present, we developed a novel class of polydopamine (PDA) coated cerium dioxide (CeO 2 ) nanozyme (Ce@P) that acts as the potent ROS scavenger for scavenging intracellular ROS and suppressing inflammatory responses against ALI. Herein, we aimed to identify that Ce@P combining with NIR irradiation could further strengthen its ROS scavenging capacity. Specifically, NIR triggered Ce@P exhibited the most potent antioxidant and anti-inflammatory behaviors in lipopolysaccharide (LPS) induced macrophages through decreasing the intracellular ROS levels, down-regulating the levels of TNF-α, IL-1β and IL-6, up-regulating the level of antioxidant cytokine (SOD-2), inducing M2 directional polarization (CD206 up-regulation), and increasing the expression level of HSP70. Besides, we performed intravenous (IV) injection of Ce@P in LPS induced ALI rat model, and found that it significantly accumulated in the lung tissue for 6 h after injection. It was also observed that Ce@P + NIR presented the superior behaviors of decreasing lung inflammation, alleviating diffuse alveolar damage, as well as promoting lung tissue repair. All in all, it has developed the strategy of using Ce@P combining with NIR irradiation for the synergistic enhanced treatment of ALI, which can serve as a promising therapeutic strategy for the clinical treatment of ROS derived diseases as well.
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Biomass Conversion and Biorefinery
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2024
·
IF 3.5
Ouyang Yue,Sun Wei,Tian Longjin,Liu Ying,Fan Guozhi,Pan Cheng,Cheng Qunpeng
In this study, the performance of biochar (BC)-supported cobalt (Co) and copper (Cu) (CoCu@BC) on tetracycline (TC) adsorption in aqueous solution and piggery wastewater was investigated. Scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET) surface area, Fourier-transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD) were used to characterize the CoCu@BC adsorbent. The influence of initial pH, TC concentration, adsorbent dosage, and co-existing ions on TC adsorption performance was studied. Meanwhile, the adsorption kinetics, isotherm, and diffusion models were studied. The results showed that the CoCu@BC was characteristic of 142.1745 m 2 ·g −1 (BET surface area), 0.035987 cm 3 ·g −1 (microspore volume), and 3.74 (pH PZC ). The adsorption of TC on CoCu@BC well fitted with the pseudo-second-order model and the Freundlich isotherm. The maximum adsorption capacity of CoCu@BC was 621.118 mg/g, which was less influenced by pH while greatly inhibited by NO 2 − and SO 4 2− . Finally, the removal of TC (94.5%), NH 4 + -N (27%), and COD (18%) by CoCu@BC was carried out in piggery wastewater, indicating its superior performance in practice application.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2024
·Vol. 50
·期 6
·2455-2476
·
IF 2.8
Yan Jia,Hu Cheng,Zhang Lianhong
Graphitic carbon nitride (g-C 3 N 4) faces limitations in its photocatalytic applications due to its inherently wide bandgap (2.7 eV), low utilization of visible light, and a high rate of recombination of photogenerated electron–hole pairs. Defect engineering can effectively enhance the ability of g-C 3 N 4 photocatalysts to address environmental pollution. In this paper, g-C 3 N 4 materials with N defects (AA-CN15) were successfully prepared by using urea as a hydrogen bond donor and ammonium acetate as a hydrogen bond acceptor. The catalyst exhibits a broader range of visible light absorption, a lower rate of photogenerated electron–hole recombination, and a larger specific surface area, thanks to the formation of N defects in the N1 (C=N–C) vacancy. The formation of N defects reduces the band gap width of AA-CN15 from 2.85 eV to 1.90 eV compared to U-CN. The degradation rate of AA-CN15 in a 30 mg/L MB solution under visible light irradiation can reach 91.4% within 100 min, which is 7.2 times higher than that of U-CN. This study addresses the limitations and drawbacks of traditional defect introduction methods, offering a novel approach for the synthesis of N defect g-C 3 N 4 materials.
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JOURNAL OF NANOBIOTECHNOLOGY
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2024
·Vol. 22
·期 1
·1-20
·
IF 10.6
Ye Lusha,Gao Yanan,Mok Simon Wing Fai,Liao Wucan,Wang Yazhou,Chen Changjiang,Yang Lijun,Zhang Junfeng,Shi Liyun
Acute lung injury (ALI) is generally caused by severe respiratory infection and characterized by overexuberant inflammatory responses and inefficient pathogens-containing, the two major processes wherein alveolar macrophages (AMs) play a central role. Dysfunctional mitochondria have been linked with distorted macrophages and hence lung disorders, but few treatments are currently available to correct these defects. Plant-derive nanovesicles have gained significant attention because of their therapeutic potential, but the targeting cells and the underlying mechanism remain elusive. We herein prepared the nanovesicles from Artemisia annua, a well-known medicinal plant with multiple attributes involving anti-inflammatory, anti-infection, and metabolism-regulating properties. By applying three mice models of acute lung injury caused by bacterial endotoxin, influenza A virus (IAV) and SARS-CoV-2 pseudovirus respectively, we showed that Artemisia-derived nanovesicles (ADNVs) substantially alleviated lung immunopathology and raised the survival rate of challenged mice. Macrophage depletion and adoptive transfer studies confirmed the requirement of AMs for ADNVs effects. We identified that gamma-aminobutyric acid (GABA) enclosed in the vesicles is a major molecular effector mediating the regulatory roles of ADNVs. Specifically, GABA acts on macrophages through GABA receptors, promoting mitochondrial gene programming and bioenergy generation, reducing oxidative stress and inflammatory signals, thereby enhancing the adaptability of AMs to inflammation resolution. Collectively, this study identifies a promising nanotherapeutics for alleviating lung pathology, and elucidates a mechanism whereby the canonical neurotransmitter modifies AMs and mitochondria to resume tissue homeostasis, which may have broader implications for treating critical pulmonary diseases such as COVID-19.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 2
·1-11
·
IF 5.3
Huang Suiyuan,Sun Xuehua,Xin Jianwei,Ma Panpan,Zhang Yuqi,Cai Zhongyu,Wang Ji-Jiang
Dexamethasone sodium phosphate (DSP) and betamethasone sodium phosphate (BSP) imprinted hydrogels embedded with two-dimensional photonic crystals (2DPC) were developed as hormones-sensitive photonic hydrogel sensors with highly sensitive, selective, anti-interference and reproducible recognition capability. The DSP/BSP molecularly imprinted photonic hydrogels (denoted as DSP-MIPH and BSP-MIPH) can specifically recognize DSP/BSP by rebinding the DSP/BET molecules to nanocavities in the hydrogel network. This recognition is enabled by the similar shape, size, and binding sites of the nanocavities to the target molecules. The rebinding of hormones molecules causes the hydrogel to swell, resulting in a particle spacing increase of the embedded 2DPC of the hydrogel. The particle spacing increase can be used as sensing signal and can be acquired by simply measuring the Debye diffraction diameters of the photonic hydrogel sensor before and after exposure with a laser pointer and a ruler. The particle spacing increments of the DSP-MIPH and BSP-MIPH sensors linearly change when DSP and BSP concentrations changes within the ranges 0.025–1 μM, 10–100 μM, and 100–500 μM, and the limits of detection (LoD) are 21.8 nM for DSP and 12.6 nM for BSP, respectively. These photonic hydrogel sensors were successfully applied to the determination of DSP/BSP contents in the real pharmaceutical injections, providing an ideal strategy for the development of portable hormones sensors. Graphical
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npj Flexible Electronics
·
2024
·Vol. 8
·期 1
·1-10
·
IF 12.3
Wang Zijian,Yu Wen,Gao Chaochao,Zhu Zhenye,Zhang Jiaheng
Fabricating high-conductive composites and constructing highly conductive networks are crucial for high-performance electrothermal film. In this study, an Ag nanowires/graphene (Ag/G) composite synthesized by liquid-phase exfoliation and in-situ photoreduction is mixed with carbon black (CB) to form a composite conductive ink, and a CB/Ag/G composite electrothermal film with a point-line-plane three-dimensional microstructure is obtained via blade coating process. Both the addition of Ag nanowires and a subsequent compression rolling treatment induce the establishment of the effective conductive network in the film, endowing it with an outstanding conductivity of 399.4 S cm −1 . The film reaches a T s of 204 °C with an input voltage of 3.0 V, and is successfully applied in water heating and de-icing, demonstrating its extraordinary electrothermal performance and vast potential for practical applications. The film is also used as an electromagnetic shielding film and heat dissipation substrate, showing exceptional electromagnetic shielding (42.5 dB) and heat dissipation properties.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2024
·
IF 2.3
Zhao Yuan,Feng Huixia,Shang Qiong,Jiao Linhong
Carbon-based hydrogels have emerged as a promising material for wearable strain and pressure sensors due to their excellent conductive and mechanical flexibility. However, some shortcomings such as limited stretchability and susceptibility to phase separation have led to a narrow range of applications. In this study, a GPEC hydrogel was prepared by incorporating metal ions and oxidized multi-walled carbon nanotubes (oxCNTs) into a double-network (DN) hydrogel consisting of gum arabic (GA) and a copolymer polymerized by acrylamide (AM), acrylic acid (AA) and N-methylolacrylamide (NMAM). The uniformly distributed oxCNTs and metal ions formed a three-dimensional (3D) structure of the hydrogel through a large amount of metal complex bonds and hydrogen bonds. The strong interaction improved the mechanical properties of the hydrogels, with an elongation at break of 1957% and a strength at break of 915 kPa. Furthermore, the hydrogels exhibited excellent self-adhesive and self-healing properties. The hydrogel also exhibits high conductivity due to the embedded metal ions and oxCNTs forming a conductive network. The as-prepared strain sensor revealed ultra-high sensitivity (GF = 3.08) and fast response (72 ms). Moreover, the GPEC hydrogel exhibits high pressure sensitivity (2.27 kPa −1 in the range of 0–10 kPa and 0.08 kPa −1 in the range of 20–80 kPa) when assembled into a pressure sensor. Consequently, the GPEC hydrogel sensor could be used to monitor the full range of human motion and could be incorporated into pressure sensing devices for handwriting recognition. Graphical Abstract
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危险品化学品经营许可证（带存储）