Frontiers in Pharmacology
·
2020
·
IF 4.225
Guo Hui,Li Faping,Qiu Heping,Liu Jianhua,Qin Sihao,Hou Yuchuan,Wang Chunxi
The poor solubility and permeability of most chemotherapeutic drugs lead to unsatisfactory bioavailability combined with insufficient drug concentration. In this study, positively charged nanoparticles based on chitosan were developed and synthesized to enhance tumor penetration capability of 10-Hydroxycamptothecin (HCPT) in order to improve the chemotherapeutic effect of melanoma. The HCPT encapsulated nanoparticles were noted as NPs/HCPT. NPs/HCPT was characterized by dynamic light scattering and zeta potential measurements. In addition, cell uptake, in vitro cytotoxicity, apoptosis and in vivo antitumor activity of NPs/HCPT were further investigated. The average diameter of NPs/HCPT was approximately 114.6 ± 4.1 nm. The viability of murine melanoma cell lines (B16F10 and B16F1) was significantly decreased due to interaction with NPs/HCPT. Moreover, NPs/HCPT significantly inhibited the progression of tumors. These investigations implied that cationic NPs/HCPT could be potentially applied as a promising drug delivery nanosystem.
✓ 已验证
DOI
PubMed
高影响因子
POLYMER TESTING
·
2019
·
IF 2.943
Lian Xing,Ye Ma,Huaping Tan,Guoliang Yuan,Shengke Li,Jianliang Li,Yang Jia,Tianle Zhou,Xiaohong Niu,Xiaohong Hu
Transparent and flexible alginate-based membranes containing antibacterial drugs were prepared by a casting/solvent evaporation method from alginate solution mixed with chitosan floccule (CSF) and oxidized sodium alginate (OAlg). To improve the properties of the membranes, CSF and OAlg were added to the alginate solution in different ratios. The mechanism of membrane formation is attributed to two aspects: Schiff-base cross-linking between CSF and OAlg; ion cross-linking between sodium alginate and CaCl 2 . The results showed that the incorporation of CSF and OAlg had a significant effect on the properties of the alginate membrane. With the change in the ratio between CSF and OAlg, the tensile strength , swelling rate, water vapor permeability, wettability and degradation stability of the membranes were significantly changed. In order to enhance the anti-bacterial properties, clindamycin (DA) and silver sulfadiazine (AgSD), which represent water-soluble and water-insoluble drug models, were integrated into the membrane, respectively. The results of controlled release effects and inhibition zones indicate that the alginate membranes containing drugs have a promising future in treatment of bacterial infection as wound dressing.
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Linyu Nian,Yao Xie,Xiaoyang Sun,Mengjun Wang,Chongjiang Cao
The postharvest physiological and metabolic activities caused fruits and vegetables (F&V) quality deterioration. Therefore, developing an efficient preservation strategy is a promising approach to relieve this issue. In this study, a modified metal-organic framework (MOF; i.e., [email protected] ) was encapsulated into a blended matrix of chitosan quaternary ammonium salt (CQAS)/gelatin to fabricate a multifunctional (water-locking, ethylene-removing, and antibacterial) packaging biopolymer-based film (i.e., CMCGF), the characteristics and preservative effects of the packaging were investigated. Results indicated that the physicochemical (e.g., mechanical, gas/light barrier, wettability) properties of CMCGF were improved compared with the control film (i.e., CGF). CMCGF have a higher ethylene adsorption performance of 65–69 cm 3 /g STP compared with CGF (7.8 cm 3 /g STP). Cu ions released from CMCGF destroyed the cell wall and membrane, resulting in the death of bacteria, and the antibacterial efficiency of CMCGF against E. coli and S. aureus was 97–100 % and 98–100 %, respectively. Postharvest storage experiments on tomato and winter jujube confirmed the high-efficiency preservation effect of CMCGF packaging. Therefore, CMCGF provides a multifunctional approach to extending the shelf-life of perishable products to decrease food wastage.
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Meiling Lian,Xu Chen,Xuejiao Liu,Zongchun Yi,Wensheng Yang
A novel peptide nanotube and chitosan composite (PNT–CS) is prepared by self-assembly of dipeptide monomers in a CS aqueous solution. A scanning electron microscopy image showed that the composite had a three-dimensional (3D) network structure. Contact angle analysis indicated good hydrophilicity of the PNT–CS. X-ray diffraction patterns and FT-IR spectra revealed that the basical crystallinity properties and assembled manner of the PNTs in the composite could be maintained. Electrochemical characterization demonstrated that the PNT–CS had good conductivity and excellent stability during the electrochemical measurements. The composite combined the advantages of the two constituent materials and was further employed as an electrochemical cytosensor for the detection of cancer cells (K562). The linear range from 5 × 10 3 to 5 × 10 7 cells mL −1 with a detection limit of 630 cells mL −1 was obtained at the PNT–CS-modified electrodes using the electrochemical impedance method. These results are comparable with the previously reported cytosensors based on conventional nanomaterials. Nevertheless, the present method is very simple and suitable for down-up fabrication. The combination of the electrochemical properties, easy preparation, and excellent biocompatibility makes the PNT–CS a promising platform for next generation cytosensors.
✓ 已验证
DOI
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Enci Mei,Cunguo Chen,Chunxiao Li,Xiaoxia Ding,Jiashe Chen,Qiaoer Xi,Sen Zhou,Jingjing Liu,Zhiming Li
Combined therapy provides a more effective method in the treatment of tumors and becomes a research hotspot. To improve treatment outcomes and reduce serious side effects, hydrogel-based local delivery was developed herein to form a drug depot in suit to eliminate tumors. Indocyanine green and imiquimod were coencapsulated in the novel temperature-sensitive chitosan hydrogel. After intratumoral injection of the hydrogel, indocyanine green that accumulated in the tumor area could induce thermal ablation of primary tumors under laser irradiation. In the presence of imiquimod, the immune effects increased the probability of complete ablation of primary tumors and inhibition of metastases. Combined with cyclophosphamide, the enhanced immunological responses would further inhibit tumors and prolong the survival time. In a word, this work offered an excellent local delivery platform that enabled a remarkable combined antitumor strategy and achieved synergistic therapeutic effects.
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Ronglu Zhang,Yuanyuan Li,Min Zhou,Cong Wang,Pengcheng Feng,Wenjun Miao,He Huang
The fact that increasing antibiotic resistance of pathogenic bacteria and a lack of new potent broad-spectrum antibiotics call for the development of alternative approaches for treating infectious diseases. With the merits of great efficacy, safety, and facile implementation, antibacterial photodynamic therapy (APDT) represents an attractive modality for this purpose. Here, we report that the newly fabricated photodynamic chitosan nano-assembly, designated CS-Ce6, could synergistically kill antibiotic-resistant bacteria with superior potency to vancomycin. CS-Ce6 nano-assembly, obtained from covalent conjugate of chlorin e6 (Ce6) with chitosan, exhibited strong association with bacteria, thus altering their morphologies and mediating great delivery efficiency of Ce6. Upon light irradiation, localized generation of singlet oxygen by CS-Ce6 nano-assembly has a remarkable bactericidal effect toward both drug-resistance Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Acinetobacter baumannii, which was greater than that the free Ce6 and antibiotics had. We also confirmed that APDT-treated MRSA neither developed resistance to APDT nor altered their resistance to methicillin. Our in vivo studies demonstrated that the CS-Ce6 nano-assembly had comparable therapeutic efficacy with vancomycin in MRSA-infected mice. These results suggest that APDT by photodynamic chitosan nano-assembly hold great potential in combating antibiotic-resistant bacteria and hopefully in reducing the need of antibiotics in the future.
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Liyun Rui,Minhao Xie,Bing Hu,Li Zhou,Danyang Yin,Xiaoxiong Zeng
Gallic acid-grafted-chitosan (GA- g -CS) was synthesized in the presence of 1-ethyl-3-(3′-dimethylaminopropyl) carbodiimide and hydroxybenzotriazole under room temperature. To develop a kind of newly functional film, CS, gelatin, glycerol and Tween 20 were utilized to prepare CS-based composite films with incorporated GA (GA-CS) or conjugated GA (GA- g -CS), and the films were characterized in aspects of physical and functional properties, as well as microstructures. With the increasing of GA concentration, there was a reduction in transmittance, elongation at break and water vapor permeability (WVP) for GA-CS film. For GA-CS films, the higher tensile strength and lower WVP could be obtained when GA was evenly distributed across the films. Different from the GA-CS films, water solubility, swelling ratio, water vapor permeability and scavenging activities on 2,2-Diphenyl-1-picrylhydrazyl/2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) radicals were enhanced for GA- g -CS films, which could be ascribed to the rough surface morphology. The GA- g -CS films exhibited enhanced water vapor permeability when the substitution degree of GA increased. Also, the antimicrobial activities of GA- g -CS films were superior to those of GA-CS films. Our present work developed a way to prepare GA- g -CS films that owned many desirable properties and could be explored as promising biomaterials in food packaging.
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 36
·15459-15466
·
IF 3.288
Lijuan Liu,Yu Ma,Weiting Yang,Chen Chen,Meiling Li,Duoyu Lin,Qinhe Pan
One of the challenges to sustainability is to efficiently and conveniently remove organic dyes from wastewater. Herein, a light ZIF-8@chitosan (CS) composite sponge with bulk density of 0.18 g cm−3 was prepared by an in situ method. The composite sponge possesses a three-dimensional porous structure with abundant chelating sites, which can efficiently and selectively remove congo red (CR). The maximum adsorption capacity (987.01 mg g−1) is higher than most chitosan-based adsorbents. High selective adsorption towards CR over other dyes is also demonstrated. Over 90% adsorption capacity was retained after five adsorption–desorption cycles. In addition, a column-mounted equipment for fixed-bed cycle wastewater treatment was assembled with a freestanding ZIF-8@CS sponge, which exhibited high CR removal efficiency of 99%. Based on the systematic spectroscopy characterization, the dominant role in the superior adsorption performance is attributed to the electrostatic interactions, hydrogen bonds, π–π stacking and metal coordinating interaction between the ZIF-8@CS sponge and CR.
✓ 已验证
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2018
·Vol. 99
·期 4
·1596-1603
·
IF 2.379
Rui Yang,Yu Jiang,Lili Xiu,Jianying Huang
BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts. The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions. RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment. The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time. Additionally, the lowest phytic acid content was observed in 8 g kg −1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg −1 , which was decreased by 79.0% compared to the value in ungerminated soybean. CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality. Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor. © 2018 Society of Chemical Industry
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Hao Li,Feng Wang
It is imperative to develop a wound dressing that can effectively treat bacterial infection in the wound location and speed up wound healing. In this study, chitosan (CS) microspheres with a core–shell structure were prepared using high-voltage electrostatic drop technology. The antimicrobial polypeptide (PonG1) and bFGF, were encapsulated in the core of the CS microsphere. The preparation of CS microspheres was studied by changing the parameters. The surface morphology of CS microspheres and the release quantities of PonG1 and bFGF were observed and detected. In vivo experiments were conducted to study the neovascularization and anti-inflammatory abilities of bFGF and PonG1. The repair ability of CS microspheres was evaluated using a rat model of full-thickness skin wound. Both bFGF and PonG1 exhibited slow release from the CS microspheres within 14 days. In vivo experiments showed that CS microspheres containing PonG1 (3 mg/ml) and bFGF (300 μg/ml) had the best neovascularization and anti-inflammatory effects. CS microspheres containing both bFGF and PonG1 had the strongest ability to repair skin wound. CS microspheres with antimicrobial and vascularized functions can be used as dressings for the repair of hard-to-heal wounds.
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
·
2020
·
IF 1.624
Kun Wu,Dan Zhao,Huifei Cui
The wound dressing can temporarily replace the skin and play a protective role in the process of wound healing, preventing wound infection and inflammation, and providing a favorable environment for wound healing. In this study, a mixture of collagen and chitosan was lyophilized to be the host material of the sponge. This sponge was soaked into 1-ethyl-(dimethylaminopropyl) carbodiimide/N-hydroxy sulfosuccinimide cross-linking solution containing heparin and experienced secondary lyophilization to prepare the heparinized sponge (CT-CL/Hp). The surface morphology and structural characterization of the sponge was characterized by scanning electron microscope and Fourier transform infrared spectrometer, respectively. Relatively favorable water absorption capability were observed by measuring the physical properties. Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro. The sustained-release property of heparin from the sponges was measured using Alcian Blue assay. Experiments in vitro and in vivo showed that the sponges had satisfactory biocompatibility and lower sensitization. Moreover, the effect of sponge on early stages of wound healing was evaluated by guinea pigs wound healing models. Analysis of wound healing rates and histological examination showed satisfactory results. CT-CL/Hp enhanced expression of growth factors, particularly VEGF and EGF at day 7. These results demonstrated that CT-CL/Hp–treated sponges benefit to wound skin healing and regeneration.
✓ 已验证
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2016
·Vol. 12
·期 8
·1688-1698
·
IF 3.929
Zhang Xueqiong,Zhang Hong,Yin Liangquan,Hu Rui,Qiu Tong,Yin Yihua,Xiong Xiong,Zheng Hua,Wang Qun
Nanoparticles, such as polymeric micelles, are currently regarded as effective drug delivery vehicles. In this study, a series of carboxymethyl chitosan/daunorubicin (CMCS/DNR) conjugates with macromolecular carriers of different molecular weights (MWs) were prepared and structurally
characterized by Fourier transform infrared spectroscopy (FT-IR) and 1H-NMR spectroscopy. The results indicated that the MWs of the carriers greatly influenced the drug loading capacity. DNR was conjugated to the polymer via an acid-sensitive hydrazone linkage susceptible to hydrolysis
at pH 5–6, thereby enabling intracellular DNR release. In aqueous media, the conjugates spontaneously formed nano-sized particles with core–shell structures (CMCS and DNR as the shell and core, respectively). Dynamic light scattering (DLS) and trans-mission electron microscopy
(TEM) indicated that the prepared nanoparticles were spherical in shape with diameters of 100–200 nm and had a slight negative surface charge. In addition, in vitro drug release studies demonstrated that the micelles were highly sensitive to mild acidic conditions (pH 5.0) but
remained reasonably stable at pH 6.5 and pH 7.4. Importantly, cell counting kit-8 (CCK-8) assays demonstrated that DNR-conjugated nanoparticles exerted a cytotoxic effect on HeLa cells, with the IC50 being approximately 2 times higher than that of free-DNR. Furthermore, confocal
laser scanning microscopy (CLSM) revealed that the CMCS-hyd-DNR nanosystem could efficiently deliver and release DNR in the nuclei of cancer cells. Taken together, the developed CMCS based pH-sensitive nanosystem may be a potential drug delivery vehicle for cancer therapy.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）