PARASITOLOGY RESEARCH
·
2021
·Vol. 120
·期 8
·2827-2837
·
IF 2.289
Guo Wenju,Li Ning,Ren Guolian,Wang RongRong,Chai Liqing,Li Yujie,Wang Xi,Yang Qingshan,Wang Ruili,Zhang Guoshun,Yang Liuqing,Yi Bofang,Zhang Shuqiu
Currently, conjugation of artemisinin-derived dimers, trimers, and tetramers is a viable strategy for developing new effective antimalarial candidates. Furthermore, nanotechnology is an effective means to achieve intravenous administration of hydrophobic drugs. In this paper, an ester-linked dihydroartemisinin trimer (DHA 3 ) was synthesized and further prepared as self-assembled nanoparticles (DHA 3 NPs) by a one-step nanoprecipitation method. The pharmacokinetics and antimalarial pharmacodynamics of DHA 3 NPs were studied in rats and mice infected with Plasmodium yoelii BY265 (PyBY265) . DHA 3 NPs had a regular spherical shape with a uniform size distribution of 140.27 ± 3.59 nm, entrapment efficiency (EE) of 99.63 ± 0.17%, and drug loading efficiency (DL) of 79.62 ± 0.11%. The in vitro release characterization revealed that DHA 3 NPs were easily hydrolysed into DHA in an esterase environment. The pharmacokinetics study demonstrated that the area under the concentration–time curve ( AUC 0-t ) of DHA in DHA 3 NPs group was 2070.52 ± 578.76 h×ng×mL −1 , which was higher than that of DHA and artesunate (AS) control groups ( AUC 0-t values of 724.18 ± 94.32 and 448.40 ± 94.45 h×ng×mL −1 , respectively) ( P < 0.05). The antimalarial pharmacodynamics in vivo suggested that DHA 3 NP S (ED 90 7.82 ± 1.16 μmol×(kg×day) −1 ) had a superior antimalarial effect compared with that of control groups (ED 90 values of 14.68 ± 0.98 (DHA) and 14.34 ± 1.96 (AS) μmol×(kg×day) −1 ) ( P < 0.05). In addition, DHA 3 NP S reduced the recurrence ratio and improved the cure ratio and survival time. In summary, DHA 3 NPs exhibited promising pharmacokinetic characteristics and antimalarial pharmacodynamics in vivo.
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Nano Research
·
2021
·Vol. 15
·期 1
·502-509
·
IF 8.897
Yang Dandan,Li Xiaoming,Li Yuelei,Cai Bo,Su Lin,Zhang Shengli,Juan Zhili,Meng Cuifang,Geng Dongling,Chen Jiaxin,Xu Feng,Zeng Haibo
Controllable anisotropic growth of perovskite nanocrystals (NCs) is challenging since it is difficult to separate the nucleation and growth processes. Here, a two-step nucleation strategy is proposed to control the binding interaction between surface ligands and NCs, resulting in facet-induced coordination competition. Oleic acid as surface activated ligand leads to the formation of defective lead bromine octahedron, and the binding interaction between 4-dodecylbenzenesulfonic acid and lead atoms promotes the formation of two kinds of binding interactions. Based on this strategy, the anisotropic growth of CsPbBr 3 nanoplatelet (NPLs) with adjusted length from 11.4 to 24 nm, and the evolution of NPLs from stacked to tongue-shaped have been realized. Elemental line scan reveals the sulfur atoms mainly distribute at the edge of NPLs. Furthermore, binding energy calculation and experimental results illustrate the coordination competition of different binding interaction on specific facets induces the anisotropic growth of NPLs. Importantly, strong emission anisotropy of highly ordered NPLs with polarization ratio up to 0.58 is illustrated. This work not only deepens our understanding of the controllable synthesis of perovskite NCs, but also provides a reference for the regulation of light emitting diode and soler cells.
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BMC MICROBIOLOGY
·
2021
·Vol. 21
·期 1
·1-12
·
IF 3.605
Miao Z. H.,Zhou W. X.,Cheng R. Y.,Liang H. J.,Jiang F. L.,Shen X.,Lu J. H.,Li M.,He F.
Background Accumulating evidence have shown that the intestinal microbiota plays an important role in prevention of host obesity and metabolism disorders. Recent studies also demonstrate that early life is the key time for the colonization of intestinal microbes in host. However, there are few studies focusing on possible association between intestinal microbiota in the early life and metabolism in adulthood. Therefore the present study was conducted to examine whether the short term antibiotic and/or probiotic exposure in early life could affect intestinal microbes and their possible long term effects on host metabolism.Results A high-fat diet resulted in glucose and lipid metabolism disorders with higher levels of visceral fat rate, insulin-resistance indices, and leptin. Exposure to ceftriaxone in early life aggravated the negative influences of a high-fat diet on mouse physiology. Orally fed TMC3115 protected mice, especially those who had received treatment throughout the whole study, from damage due to a high-fat diet, such as increases in levels of fasting blood glucose and serum levels of insulin, leptin, and IR indices. Exposure to ceftriaxone during the first 2 weeks of life was linked to dysbiosis of the fecal microbiota with a significant decrease in the species richness and diversity. However, the influence of orally fed ceftriaxone on the fecal microbiota was limited to 12 weeks after the termination of treatment. Of note, at week 12 there were still some differences in the composition of intestinal microbiota between mice provided with high fat diet and antibiotic exposure and those only fed a high fat diet.Conclusion sThese results indicated that exposure to antibiotics, such as ceftriaxone, in early life may aggravate the negative influences of a high-fat diet on the physiology of the host animal. These results also suggest that the crosstalk between the host and their intestinal microbiota in early life may be more important than that in adulthood, even though the same intestinal microbes are present in adulthood.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 14
·18551-18564
·
IF 2.478
Wang J. L.,Shang J. H.,Guo Y. J.,Jiang Y. Y.,Xiong W. K.,Li J. S.,Yang X.,Torun H.,Fu Y. Q.,Zu X. T.
A surface acoustic wave (SAW) formaldehyde gas sensor was fabricated on a 42° 75′ ST-cut quartz substrate, with a composite sensing layer of zeolitic imidazolate framework (ZIF)-8 on polyethyleneimine (PEI)/bacterial cellulose (BC) nanofilms. The addition of snowflake-like ZIF-8 structure on the PEI/BC sensitive film significantly improves the hydrophobicity of the SAW sensor and increases its sensitivity to formaldehyde gas. It also significantly increases surface roughness of the sensitive film. The hydrophobic nature of ZIF-8 prevents water molecules from entering into the internal pores of the BC film, thereby avoiding a significant mass loading caused by humidity when the sensor is used to detect low-concentration formaldehyde gas. The Zn 2+ sites at the surface of ZIF-8 improves the sensor's response to formaldehyde gas through enhanced physical adsorptions of gas molecules. Experimental results show that the ZIF-8@PEI/BC SAW sensor has a response (e.g., frequency shift) of 40.3 kHz to 10 ppm formaldehyde gas at 25 °C and 30% relative humidity (RH). When the relative humidity is increased from 30 to 93%, the response of the sensor only varies ~ 5%, and the change in response is negligible at medium humidity levels (~ 50 to 60% RH).
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JOURNAL OF ELECTRONIC MATERIALS
·
2021
·Vol. 50
·期 5
·2789-2799
·
IF 1.938
Fahad Shah,Yu Haojie,Wang Li,Wang Yang,Lin Tengfei,Amin Bilal Ul,Naveed Kaleem-Ur-Rahman,Khan Rizwan Ullah,Mehmood Sahid,Haq Fazal,Xing Yusheng,Usman Muhammad
High molecular weight polyvinylpyrrolidone (PVP) and seeding conditions are considered to have important roles in the synthesis of AgNWs. In this work, we report a versatile synthesis of AgNWs in a salt-free environment and under Ar inert atmosphere using high molecular weight PVP and seeding step during the synthetic process. The high molecular weight PVP was found to efficiently promote the preparation of AgNWs via supporting the proper growth control of AgNWs on {111} facets and selective growth on {100} facets. The seeding step facilitates the formation of seeds such as multiple twinned nanoparticles (MTPs) and single crystal seeds, which promoted the feasible formation of AgNWs. The shape and size of the synthesized AgNWs were characterized by SEM and TEM. The crystalline nature of the synthesized AgNWs was characterized by HRTEM, SAED and XRD, and their LSPR peaks were confirmed by UV-Vis spectroscopy. The conductive properties of the synthesized AgNWs were determined by preparing their thin conductive film on a PET substrate. The prepared AgNWs thin films exhibited good sheet resistance.
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MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2021
·Vol. 476
·期 6
·2479-2489
·
IF 3.396
Chai Qian,Meng Ziang,Lu Dexue,Zhang Ziying,Liu Meili,Wu Weihua
Cardiomyocyte death is an important pathogenic process in cardiac complications of diabetes. Diabetic patients often suffer glycemic variability. Pyroptosis is a form of programmed cell death triggered by inflammasomes and related with caspase-1 and gasdermin D activation. The present study was designed to examine the effects of intermittent high glucose simulating glycemic variability on the pyroptosis of cardiomyocytes in vitro. Rat H9C2 cardiomyocytes were incubated with normal glucose (NG), constant high glucose (CHG) and intermittent high glucose (IHG). Results showed that compared to CHG treatment, IHG further inhibited cell proliferation and promoted cell death of H9C2 cardiomyocytes. In addition, IHG upregulated higher levels of the expressions of inflammasome NLR family pyrin domain containing 3 (NLRP3) and adaptor protein apoptosis-associated speck-like protein containing CARD domain (ASC) and increased higher levels of activated caspase-1 and gasdermin D than CHG treatment. Moreover, the production of reactive oxygen species (ROS) and activation of NF-κB that is induced by IHG were significantly higher than that induced by CHG. Knockdown of sodium–glucose cotransporters 1 (SGLT1) in H9C2 cardiomyocytes was performed and the effects of SGLT1 on IHG-induced pyroptosis was evaluated. The results demonstrated that knockdown of SGLT1 partially reduced IHG-induced pyroptosis, ROS generation and NF-κB activation. Our results indicated that IHG is harmful to cardiomyocytes and it might be partially because of the SGLT1-depedent pyroptosis in cardiomyocytes.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 5
·6064-6079
·
IF 2.478
Xu Jing-Wen,Ma Ting-Mei,Zhang Mi,Zeng Han
Multi-wall carbon nanotubes (MWCNTs) with polypyrrole (Ppy) attachment were selected to play the role of supporter to accommodate hemoglobin (Hb). Metalloprotein-based electrode was prepared on the basis of MWCNTs-Ppy nano-complex with hemoglobin entrapment via conventional wet-coating. Morphology, formation parameters and physio-chemical characteristics of nano-complex with hemoglobin detainment were systematically characterized and analyzed by microscopy, spectrometry and electro-chemistry. Results from measurements and tests implied that the direct electron transferring of Hb-based electrode could be achieved with redox group in Ppy as primary electro-active site and heme sites of Hb in coordination with Ppy as intra-molecular relay. Redox process for heme site of Hb in ligation with Ppy should be attributed to a surface-controlling quasi-reversible electro-chemical reaction with 2 H + and 2 electron involvement. Such abutting complexation would not impose considerable negative effect on the kinetics of substrate (H 2 O 2 ) combination and subsequent its transformation. The efficiency of Hb-induced catalysis was limited by the step of substrate diffusion.
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AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 1
·1-9
·
IF 3.246
Sun Xuechun,Zheng Xin,Tang Yan,Debrah Augustine Atta,Du Zhenxia
Biological activity and pharmacological efficacy of protein drugs may be affected by the compatibility between drug and packaging materials. The compatibility of rubber closures seal cap has become the focus of many studies due to its complicated formulation. Despite of the significance of the issue, currently, there is little available data about organic leachables in drugs which is also not comprehensive. Since the concentration of migrants in drug is usually low and the matrix is complicated, the establishment of overall profile of extractables is crucial for the characterization of leachables. Herein, the supercritical fluid extraction (SFE) method was used because of its great extraction capacity and efficiency for low to medium polar extractables in rubber stoppers. The SFE conditions were optimized by response surface methodology (RSM). Experimental results of the extract yield were close to the predicted values ( R 2 = 0.95). Then the extractables were qualitatively and quantitatively analyzed with ultrahigh performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF MS). Finally, risk assessment was made by comparing predicted exposure with injection permitted daily exposure (pPDE) limit or threshold recommended by threshold of toxicological concern (TTC). The results showed that there are many extractables such as glyceride, fatty acids and derivatives, antioxidants, and degradation products. Among them degradation products were in the majority and content of 17 substances exceeded corresponding limits. Considering their unknown toxicology, more experiments are therefore needed to provide information on their toxicology and risk assessment. The study provides a reference for the compatibility of drugs, and quality supervision of pharmaceuticals packaging.
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KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2020
·Vol. 37
·期 12
·2143-2151
·
IF 2.69
Shi Guangyu,Wang Yuanhao,Cao Yafeng,Cai Weijie,Tan Fengzhi
A Ni/Ce 0.8 Zr 0.2 O 2 catalyst (NiCeZr-N) was synthesized by a facile co-nanocasting technique for syngas production from ethanol dry reforming. In addition, a series of characterization techniques, such as transmission electron microscopy (TEM), X-ray diffraction (XRD), inductive coupled plasma Emission Spectrometer (ICP), X-ray photoelectron spectroscopy (XPS), Raman and hydrogen temperature programmed reduction (H 2 -TPR) were selected to evaluate the physicochemical features of the as-prepared catalysts. Indeed, the results indicated that NiCeZr-N catalyst prepared by co-nanocasting method had a smaller particle size (<5 nm), relatively higher specific surface area (39 m 2 /g) and stronger metal-support interaction in comparison with another model catalyst obtained from conventional co-precipitation method (NiCeZr-P). Expectedly, these positive factors enabled NiCeZr-N catalyst to exhibit better activity and stability. Typically, ethanol is completely converted by using NiCeZr-N as catalyst and heating to 700 °C, and CO 2 conversion was as high as 65.3%. Interestingly, H 2 /CO was close to 1.1 at 650 °C, which could be used as feedstocks of Fischer-Tropsch process. Particularly, no obvious fluctuation of ethanol conversion and the product selectivity was observed during 40 h time-on-stream stability test.
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Reaction Kinetics Mechanisms and Catalysis
·
2020
·Vol. 131
·期 2
·997-1007
·
IF 1.52
Li Yan,Jian Li-Juan,Li Xue,Liu Fang-Ting,Dong Xiao-Fei,Wang Jian,Zhao Yun,Wang Cheng-Wei
A new sphere-like nano-heterostructure of Cu 3 SnS 4 @C (CTS@C) was well designed and fabricated by two-step hydrothermal route, which is ideally suited for synergistic physical adsorption and photodegradation of anionic dye. Compared to the pure C or CTS nanospheres, CTS@C nano-heterostructure has smaller surface area but exhibits excellent performance. It possesses high adsorption efficiency of 97.04% for removal of methyl orange (MO) at pH 5.5. And also, the superior synergistic adsorption and photodegradation performance makes it exhibit 99.38% degradation efficiency within 210 min at pH 7.5, which is about 1.8 times higher than that of simplex adsorption (56.23%). These might be attributed to the higher light harvesting, effective charge separation, lower charge-transfer resistance and high photocurrent response properties of CTS@C nanostructures. Moreover, the CTS@C nano-heterostructured photocatalyst can effectively avoid surface passivation during the process of synergistic adsorption and photodegradation, and be easily regenerated from the reaction system for recycling, suggesting a promising candidates for synergistic adsorption and photocatalysis applications.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 28
·期 4
·4262-4275
·
IF 3.056
Li Chao,Luo Miaomiao,Zhou Shihua,He Hanyue,Cao Jiashun,Luo Jingyang
l -Threonine and three kinds of conductive polymers were applied for anode modification in microbial fuel cells (MFCs) for decolorization of Congo red with simultaneous electricity generation. The description of modified anodes with FTIR, surface contact angle, and CV analysis showed that the anode surface was successfully grafted with functional groups, with improving wettability, as well as the increasing specific surface area and electrochemical activity. For l -threonine modification, the highest decolorization rate of 97% of the MFC, and meanwhile, the maximum current density of 155.8 mA/m 2 , was obtained at the modified concentration of 400 mg/L. For conductive polymer modifications, the poly (aniline-1,8-diaminonaphthalene) (short for PANDAN) owned the highest performance, with the current density 185 mA/m 2 , and the decolorization rate was 97%. Compared with l -threonine, the modifications by conductive polymers were more suitable for MFC decolorization due to their functional groups and unique conductivity. In addition, high-throughput sequencing analysis was conducted for the conductive polymers modified anodes to reveal their bioelectrochemical mechanisms.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-9
·
IF 6.232
Su Zixian,Zhao Guangying,Dou Wenchao
Aflatoxin M1 (AFM1) residues in milk pose a major threat to human health, so there is an urgent need for a simple, rapid, and sensitive method for the determination of trace AFM1 in milk. In this study, a competitive immunochromatographic assay (ICA), using visual (PEI/PSS) 4 red silica nanoparticles (SiNPs) as signal amplification probes, was used for the highly sensitive detection of AFM1. The (PEI/PSS) 4 red SiNPs were used to label AFM1 monoclonal antibody (mAb) to prepare ICA for the detection of AFM1. After exploring the optimal conditions of mAb and immunoprobe dosage conditions, the lowest visual detection limit (VDL) of AFM1 in phosphate-buffered saline with Tween 20 (PBST, 10 mM, pH 7.4, containing 1% BSA, 3% sucrose, 1% trehalose, and 0.5% Tween 20) can reach 0.1 pg/mL. The intuitive visually visible value of AFM1 in both PBST and milk was 10 pg/mL. The results showed that the immunochromatographic system based on high chroma color (PEI/PSS) 4 red SiNPs has high sensitivity and broad application prospects for the detection of trace AFM1 residues in milk. The high chroma (PEI/PSS) 4 red SiNPs are expected to be a convenient biomarker for improving the sensitivity of immune chromatography bands.
The schematic diagram shows the detection principle. In this work, in the competitive experiment, (PEI/PSS) 4 red SiNPs were selected as visual labeling materials, and the specific antibody combined with the labeled material was selected as an immune probe. The AFM1-BSA antigen coupled with the macromolecular BSA was fixed on the T line of the nitrocellulose (NC) membrane. The AFM1 in sample solution competes with AFM1-BSA for the specific binding site of immune probe. The detection sensitivity of this method for AFM1 is obtained by judging the change of the red signal intensity produced by the positive sample, compared with the color at the T line of the negative sample.
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危险品化学品经营许可证（带存储）