INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Xueying Song,Lei Wang,Ting Liu,Yongfeng Liu,Xiaoxia Wu,Liu Liu
Mandarin ( Citrus reticulata L.) essential oil (MEO) reportedly displays excellent antimicrobial properties . In this study, MEO was loaded into chitosan nanoparticles (CSNPs). The characteristics, antibacterial properties and benefit in pork preservation of MEO-CSNPs were evaluated. The MEO-CSNPs displayed an excellent encapsulation efficiency (EE) (67.32%–82.35%), the particle size values of 131.3 nm–161.9 nm, and the absolute zeta potential values above 30 mV. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, and thermogravimetric analysis (TGA) revealed that the MEO was incorporated into CSNPs without requiring a chemical reaction, the antibacterial activity of the MEO remained. Furthermore, the damage of MEO-chitosan nanoemulsions (MEO-CSs) to the cell membranes of Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) was confirmed by the change of bacterial cell morphology. The anti-biofilm assays verified that the MEO-CSs substantially inhibited biofilm formation and destroyed the mature biofilms. MEO-CSs were also applied to pork, proving a great potential for pork preservation. This study provides a potential approach for developing and utilizing MEO-CSs as natural antimicrobial agents in the food industry.
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ANALYTICA CHIMICA ACTA
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2021
·
IF 6.558
Jing Wen,Lijun Zhou,Ding Jiang,Xueling Shan,Wenchang Wang,Hiroshi Shiigi,Zhidong Chen
Herein, a sensitive and selective electrochemiluminescence (ECL) aptasensor was designed using [email protected] as accelerator towards the perylene derivative (PTC-Cys)/peroxydisulfate (S 2 O 8 2− ) system for kanamycin (KAN) assay. Firstly, the PTC-Cys was prepared by covalently binding l -cysteine to 3,4,9,10-perylenete-tracarboxylic acid, which was acted as the luminophore. Then [email protected] play the part of effective catalyst to accelerate the electrochemical reduction process of S 2 O 8 2− to produce more sulfate radical anions (SO 4 •- ), thus the ECL signal of the compound was noticeably raised by 2.4 times in comparison with that in which only luminophore and S 2 O 8 2− are present, achieving signal amplification of the ECL system. In the presence of KAN, aptamer was pulled down from the sensing interface, achieving a considerable enhancement of ECL intensity in S 2 O 8 2− solution. Upon the optimal condition, our proposed strategy can quantify the concentration of KAN from 1.0 × 10 −13 to 1.0 × 10 −8 M with low limit of detection of 4.2 × 10 −14 M (S/N = 3).Besides, our proposed ECL aptasensor exhibited excellent sensitivity, stability and specificity, and could be successfully applied to detect KAN in practical samples, which proved its potential to detect other antibiotics in food security.
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CHEMICAL ENGINEERING JOURNAL
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2021
·
IF 13.273
Xin Zhou,Bowen Li,Mingming Guo,Wenchang Peng,Deping Wang,Qinglu Guo,Shuchao Wang,Dong Ming,Bin Zheng
Controlling medication according to the condition has become a universal treatment regimen for Parkinson's disease (PD) worldwide. However, the general oral administration method is prone to a variety of digestive system discomfort, easy to be broken down by digestive juices and intestinal microorganisms, resulting in low absorption efficiency, and a serious delay in the efficacy of the drug. Here, we developed a microneedle patch (MNP) that could release drugs with controlled temporal and spatial precision triggered by near-infrared (NIR) light for the treatment of PD. The microneedles (MNs) took Gelatin-methacryloy (GelMA) as the matrix, and therapeutic drugs (L-DOPA) were stored in the mesopore of the upconversion micron-rods (UCMRs), dispersing in the GelMA. The molecular motor, as a poreblocker and gate switch, isomerizes under the excitation of ultraviolet and visible light from UCMRs for controlling drug release. This kind of MNP penetrates the epidermis in a painless, non-invasive, and non-infectious way. Under the exposure of NIR light, the L-DOPA in the MNs will be released as needed, and then it seeps into the blood and brain for relieving the symptoms of PD. In the treatment of Parkinson's mice model, this regimen significantly restored motor function, and further mechanistic studies revealed that it reduced neuroinflammation and dopaminergic neuronal death in the substantia nigra. Besides, the released L-DOPA directly entered the blood, which reduces the side effects on the gastrointestinal tract and improves the utilization rate of the drug. These attractive superiorities indicated that the MNP with controlled drug delivery system may be candidate strategies for the treatment of PD and can be used in various related biomedical fields.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Linan Zhou,Ziteng Song,Shaojie Zhang,Yeling Li,Jing Xu,Yuanqiang Guo
Selenium nanoparticles (SeNPs), a potential cancer therapeutic agent, have attracted widespread attention owing to their high bioavailability and remarkable anticancer activity. Nevertheless, the poor water solubility and dispersibility of SeNPs seriously limit their applications. In the present study, we synthesized stable and individual spherical selenium nanoparticles (CL90-Tw-SeNP2) with an average diameter of approximately 79 nm using a polysaccharide extracted from Citrus limon (CL90) and Tween-80 as the decorator and stabilizers. The proportion of selenium in CL90-Tw-SeNP2 was 10.6%. CL90-Tw-SeNP2 possessed high stability and good dispersion in water for more than three months. The subsequent biological assay revealed that CL90-Tw-SeNP2 showed remarkable antitumor effects against HepG2 cells, with an IC 50 value of 49.13 μg/mL, by inducing cell apoptosis. Furthermore, an in vivo zebrafish assay to explore possible applications indicated that CL90-Tw-SeNP2 could inhibit the proliferation and migration of tumors and the zebrafish angiogenesis. These results indicated that CL90-Tw-SeNP2 could be a potential agent for cancer treatment, especially against human liver hepatoma cancer.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Xiuheng Xue,Haiyong He,Cunjun Liu,Li Wang,Lu Wang,Yueji Wang,Luping Wang,Chen Yang,Juhua Wang,Ruyan Hou
Diacylglycerol (DAG) is commonly used as fat substitute in food manufacture due to its functional properties, but DAG has poor emulsification and oxidation stability, which limits its wide application in food industry. In this work, fluorescence quenching data and thermodynamic parameters were analyzed to investigate the interaction mechanism between l -theanine (L-Th) and β-lactoglobulin (β-LG). DAG emulsion was prepared by using β-lactoglobulin-theanine (β-LG-Th) as surface stabilizer, and its emulsification and oxidation stability were evaluated. The results showed that the hydrophobic interaction played an important role on the conjugate of β-LG and L-Th due to the negative values for ΔG, positive values for ΔH and ΔS at pH 4.0, pH 6.0 and pH 8.0. The DAG has been better embedded by using β-LG-Th as surface stabilizer, and the droplet size was about 0.2 µm to 1.5 µm when the pH was 6.0, the ratio of L-Th to β-LG was 1:1. β-LG-Th as surface stabilizer for DAG can increase the ζ-potential and emulsion index, make the emulsion droplet size distribution more uniform. The l -theanine was better to be used to improve the emulsification stability and antioxidant capacity of DAG by binding β-LG as surface stabilizer.
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CHEMOSPHERE
·
2019
·
IF 5.108
Tianlang Zhang,Junlang Qiu,Guosheng Chen,Jianqiao Xu,Fang Zhu,Gangfeng Ouyang
In recent years, pharmaceuticals have received increasing attentions because of their potential risks to the environment, but researches focusing on their impacts on defense system of living plants are still lacking. As an important class of phytohormones, jasmonates play crucial roles in plant defense system against environmental stress. In order to investigate the effect of pharmaceuticals uptake on endogenous jasmonates, an in vivo solid phase microextraction (SPME) method was established to simultaneously detect and monitor both pharmaceuticals and jasmonates in living plants. The proposed method exhibited wide linear ranges, high sensitivity (limits of detection ranging 0.0043–0.035 ng g −1 for pharmaceuticals and 0.091–0.22 ng g −1 for jasmonates, respectively), and satisfactory reproducibility (relative standard deviation of intrafiber ranging 4.2%–8.6% and interfiber ranging 5.2%–8.2%, respectively). Subsequently, this method was successfully applied to track the concentrations of each pharmaceutical and corresponding jasmonates in living Malabar spinach plants ( Basella alba. L ) exposed to three common pharmaceuticals (i.e. gemfibrozil, mefenamic acid and tolfenamic acid) over 15 days. In result, all pharmaceuticals appeared to trigger intensive biosynthesis of jasmonic acid (JA) (3.1–9.4 times of control) while reduced the concentration of methyl jasmonate (MeJA) (18.3%–38.1% of control). We inferred that uptake of pharmaceuticals acted as an abiotic stress and stimulated the plant defense response because of the variation of jasmonates. To the best of our knowledge, this is the first study applying SPME to detect and track both pharmaceuticals and phytohormones in living plants, which not only provided a glimpse to the adverse effect of pharmaceuticals on plants as well as the regulation of endogenous jasmonates, but also set a promising template for future in vivo analysis of xenobiotics and plant endogenous substances.
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Antioxidants
·
2019
·Vol. 8
·期 10
·425
·
IF 4.52
Mengyang Hou,Wenzhong Hu,Aosheng Wang,Zhilong Xiu,Yusheng Shi,Kexin Hao,Xingsheng Sun,Duo Cao,Ruishan Lu,Jiao Sun
In the present work, the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) fromPteris creticaL. was optimized by response surface methodology (RSM) on the basis of a single-factor experiment. The optimized UAE parameters were as follows: Ethanol concentration 56.74%, extraction time 45.94 min, extraction temperature 74.27 °C, and liquid/solid ratio 33.69 mL/g. Under the optimized conditions, the total flavonoids yield (TFY) was 4.71 ± 0.04%, which was higher than that obtained by heat reflux extraction (HRE). The extracts were further analyzed by HPLC, and five major flavonoids, including rutin, quercitrin, luteolin, apigenin, and luteolin-7-O-glucoside, were identified and quantified. Furthermore, the results of the antioxidant test showed that the TF extract obtained under optimized UAE conditions exhibited good 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS+•), nitric oxide radical (NO•) scavenging activities, and ferrous ion (Fe2+) chelating capacity, with IC50values of 74.49, 82.92, 89.12, and 713.41 µg/mL, respectively. Results indicated that the UAE technique developed in this work was an efficient, rapid, and simple approach for the extraction of flavonoids with antioxidant activity fromP. cretica.Keywords:Pteris creticaL.;flavonoids;ultrasonic-assisted extraction;optimization;response surface methodology;HPLC analysis;antioxidant activity
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MOLECULES
·
2019
·Vol. 24
·期 8
·1454
·
IF 3.06
Fang-Rong Cheng,Hong-Xin Cui,Ji-Li Fang,Ke Yuan,Ying Guo
Rheum palmatumL. is a traditional Chinese medicine with various pharmacological properties, including anti-inflammatory, antibacterial, and detoxification effects. In this study, the mechanism of the hypoglycemic effect of purified anthraquinone-Glycoside fromRheum palmatumL. (PAGR) in streptozotocin (STZ) and high-fat diet induced type 2 diabetes mellitus (T2DM) in rats was investigated. The rats were randomly divided into normal (NC), T2DM, metformin (Met), low, middle (Mid), and high (Hig) does of PAGR groups. After six weeks of continuous administration of PAGR, the serum indices and tissue protein expression were determined, and the pathological changes in liver, kidney, and pancreas tissues were observed. The results showed that compared with the type 2 diabetes mellitus group, the fasting blood glucose (FBG), total cholesterol (TC), and triglyceride (TG) levels in the serum of rats in the PAGR treatment groups were significantly decreased, while superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) levels were noticeably increased. The expression of Fas ligand (FasL), cytochrome C (Cyt-c), and caspase-3 in pancreatic tissue was obviously decreased, and the pathological damage to the liver, kidney, and pancreas was improved. These indicate that PAGR can reduce oxidative stress in rats with diabetes mellitus by improving blood lipid metabolism and enhancing their antioxidant capacity, thereby regulating the mitochondrial apoptotic pathway to inhibitβ-cell apoptosis and improve β-cell function. Furthermore, it can regulate Fas/FasL-mediated apoptosis signaling pathway to inhibit β-cell apoptosis, thereby lowering blood glucose levels and improving T2DM.Keywords:Rheum palmatumL.;type 2 diabetes mellitus;oxidative stress;apoptosis
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JOURNAL OF MOLECULAR LIQUIDS
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2019
·
IF 4.561
Zhigang Yin,Longhua Xu,Jianyong He,Houqin Wu,Shuai Fang,Sultan Ahmed Khoso,Yuehua Hu,Wei Sun
Herein, we employed a range of instrumental techniques to investigate the performance of l -cysteine as a biodegradable and selective depressant in the flotation separation of molybdenite (MoS 2 ) from galena (PbS), demonstrating that this amino acid exhibited a superior depressing efficiency toward PbS and enabled the separation of the above minerals under alkaline conditions. In the presence of l -cysteine, the zeta potentials of PbS shifted to more negative values, which was indicative of chemisorption . Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy measurements indicated that the above chemisorption involved the formation of a five-membered chelate ring via coordination of Pb by S (–SH) and N (–NH 2 ) atoms, as was further confirmed by time-of-flight secondary ion mass spectrometry measurements. Moreover, the detection of l -cysteine dimer on the surface of PbS was ascribed to the occurrence of oxidation processes. The predicted adsorption modes suggested an appreciable extent of surface interaction between l -cysteine and PbS, whereas density functional theory calculations demonstrated that the interaction between l -cysteine and the PbS (100) slab corresponds to both physical and chemical adsorption.
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BIOPOLYMERS
·
2019
·Vol. 110
·期 9
·e23318
·
IF 1.845
Yan Xiao,Chenna Tang,Yang Chen,Meidong Lang
Stimuli-responsive polymers that can undergo conformational changes with external triggers have enabled themselves as smart materials for various utilizations, among which biodegradability is of particular importance to be engineered for biomedical application. In this study, a thermo and pH dual responsive polypeptide ( N , N -dimethylaminoethyl acrylate-modified poly( l -cysteine)) (PLC- g -DMAEA) was prepared by the combination of N -carboxyanhydride ring-open polymerization and thiol-ene click chemistry. The biodegradable poly( l -cysteine) (PLC) with pendant thiol groups provided an easily clickable backbone for postmodification, which was demonstrated by reacting with a well-known monomer of N , N -dimethylaminoethyl acrylate (DMAEA) to achieve both temperature and pH responsiveness. The irreversible thermo-response of PLC- g -DMAEA could be attributed to the ordered β-sheets formed upon heating, leading to the trapped side groups with poor water accessibility. Moreover, this copolymer precipitated at pH ranging from 7.5 to 9.7, but protonation of tertiary amine groups (pH < 7.5) and salt forming of masked thiol groups (pH > 9.7) rendered it soluble in water. Our results revealed that a ready available vinyl monomer could be easily clicked onto the biodegradable PLC and its stimuli responsiveness would be reserved. Moreover, the primary and secondary structures of PLC might influence the conformation, thus leading to the unique responsive behavior of the resulted copolymer. Graphical
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CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Daofei Lv,JiaYu Chen,Kexin Yang,Houxiao Wu,Yongwei Chen,Chongxiong Duan,Ying Wu,Jing Xiao,Hongxia Xi,Zhong Li,Qibin Xia
Using porous materials to selectively adsorb CO 2 from flue gas or natural gas is a promising method for mitigating CO 2 emission and purifying methane. Here, we synthesized an L-aspartic acid based microporous metal-organic framework (MIP-202) and studied its adsorptive separation performance for CO 2 /CH 4 and CO 2 /N 2 mixtures. Results show that MIP-202 had ultrahigh CO 2 /CH 4 (72.9 and 241.5 for CO 2 /CH 4 = 50/50 and 10/90, respectively) and CO 2 /N 2 (1,950,000 and 2129.1 for CO 2 /N 2 = 50/50 and 15/85, respectively) IAST selectivities at 298 K and 100 kPa. The high selectivity was verified by the calculations of Henery’s law selectivity and breakthrough experiments. Metropolis Monte Carlo simulation calculations show that CO 2 with greater polarizability and quadruple moment tends to occupy the pore walls of large cages with higher polarity, while the less polar CH 4 or N 2 majorly being adsorbed in the pore walls of small cages with lower polarity, resulting in the ultrahigh CO 2 /CH 4 and CO 2 /N 2 separation selectivities. MIP-202 exhibits low CO 2 adsorption enthalpy (17.2–30.7 kJ/mol), superior persistent reusability after five cyclic adsorption experiments, easy desorption performance at 298 K, moderate water and moisture stability, good stability under dry and humid SO 2 atmosphere, and low ligand cost ($36/kg). MIP-202 extrudates were prepared by an extrusion molding method using hydroxypropyl cellulose (HPC) as the binder and they retained 97.35% CO 2 uptake of MIP-202 powder at room temperature and pressure. This work shows that MIP-202 is an industrially promising material for the separation of CO 2 /CH 4 and CO 2 /N 2 mixtures.
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2020
·
IF 1.415
Li Jun Li,Xiao Qing Liu,Xi Ping Du,Ling Wu,Ze Dong Jiang,Hui Ni,Qing Biao Li,Feng Chen
Isoquercitrin is a flavonoid with important applications in the pharmaceutical and nutraceutical industries. However, a low yield and high production cost hinders the industrial preparation of isoquercitrin. In the present study, isoquercitrin was prepared by biotransformation of rutin using α-L-rhamnosidase from Aspergillus niger JMU-TS528 combined with high-speed countercurrent chromatography (HSCCC) purification. As a result, the optimum transformation pH, temperature, and time were pH 4.0, 60 °C, and 60 min, respectively. The Km and Vmax were 0.36 mM and 0.460 mmol/min, respectively. For isoquercitrin production, the optimal rutin concentration and transformation time were approximately 1000 mg/L and 60 min. The rutin transformation rate reached 96.44%. The isoquercitrin was purified to a purity of 97.83% using one-step purification with HSCCC. The isoquercitrin was identified using UPLC-Q-TOF-MS. The comprehensive results indicated that the biotransformation procedure using the α-L-rhamnosidase from A. niger JMU-TS528 combined with HSCCC was a simple and effective process to prepare isoquercitrin, which might facilitate the production of isoquercitrin for industrial use.
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