MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jianghui Zheng,Cuicui Fang,Mengyu Tao,Bingyong Lin,Yueliang Wang,Yuanyuan Yao,Lifen Chen,Hong Huang,Jianguo Xu,Longhua Guo
Dual-signal cross-validation enhances the reliability of quantitative measurements. However, asynchronous dual signals generated from different incident light affect the acquisition time and reliability of quantitative detection. In this work, we proposed an immunochromatography (ICA) sensor that utilizes synchronized Surface-Enhanced Raman Scattering (SERS) and temperature dual signals generated from the same incident light for analyzing microcystin-LR (MC-LR) in aquatic products. Quantitative analysis reveals a good linear correlation between the dual signals and the logarithmic concentration of MC-LR over the range of 0.001000–80.00 ng/mL. Moreover, the proposed method demonstrated excellent recovery rates and the results were similar with enzyme-linked immunosorbent assay, but the signal acquisition time (3 s) and overall detection time (10 mins) of our approach were significantly reduced. Therefore, a synchronized dual signals ICA sensor that combines SERS and photothermal technology was constructed for direct, rapid, and reliable detection of MC-LR, providing a promising application for field-based monitoring.
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2025
·Vol. 15
·期 1
·52
·
IF 5.6
Qi Yuan,Yunqing Wang
High-sensitivity and repeatable detection of hydrophobic molecules through the surface-enhanced Raman scattering (SERS) technique is a tough challenge because of their weak adsorption and non-uniform distribution on SERS substrates. In this research, we present a simple self-assembly protocol for monolayer SERS mediated by 6-deoxy-6-thio-β-cyclodextrin (β-CD-SH). This protocol allows for the rapid assembly of a compact silver nanoparticle (Ag NP) monolayer at the oil/water interface within 40 s, while entrapping analyte molecules within hotspots. The proposed method shows general applicability for detecting hydrophobic molecules, exemplified as Nile blue, Nile red, fluconazole, carbendazim, benz[a]anthracene, and bisphenol A. The detection limits range from 10−6to 10−9M, and the relative standard deviations (RSDs) of signal intensity are less than 10%. Moreover, this method was used to investigate the release behaviors of a hydrophobic pollutant (Nile blue) adsorbed on the nanoplastic surface in the water environment. The results suggest that elevated temperatures, increased salinities, and the coexistence of fulvic acid promote the release of Nile blue. This simple and fast protocol overcomes the difficulties related to hotspot accessibility and detection repeatability for hydrophobic analytes, holding out extensive application prospects in environmental monitoring and chemical analysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Boya Ma,Wenshi Zhao,Yu Wu,Xiangyue Wan,Hang Zhao,Yongdan Wang,Jihui Lang,Jinying Li,Yang Liu
Tetracycline (TC), a widely used broad-spectrum antibiotic for treating bacterial infections, poses significant risks to human health and ecological environments caused by extensive abuse. Hence, sensitive detection and efficient degradation of TC are crucial for protecting both human health and ecological environment. In this work, a multifunctional Z-scheme Cu 2 O@Ag/g-C 3 N 4 heterojunction was designed and constructed in order to achieve both ultrasensitive surface-enhanced Raman scattering (SERS) detection and efficient photocatalytic degradation of TC. The influence of the Ag content on the SERS performance was investigated using the finite-difference time-domain (FDTD) method. The charge transfer (CT) mechanism was deduced by band gap and Fermi level calculations. The results showed that the synergistic effect of electromagnetic enhancement (EM) and chemical enhancement (CE) contributed to the excellent SERS sensitivity of Cu 2 O@Ag/g-C 3 N 4 nanocomposites (NCs). The limit of detection (LOD) for TC using Cu 2 O@Ag/g-C 3 N 4 NCs as SERS substrate was as low as 10 −12 M, and the SERS substrate could also be used to TC detection in real milk samples. In addition, Cu 2 O@Ag/g-C 3 N 4 NCs could also act as the efficient photocatalyst to achieve the removal of TC with degradation rate of 94 % under visible light irradiation. Especially, the Cu 2 O@Ag/g-C 3 N 4 NCs maintained excellent photocatalytic performance even after six cycles. Possible degradation pathways and photocatalytic degradation mechanism of TC catalyzed by Cu 2 O@Ag/g-C 3 N 4 NCs were also proposed. This multifunctional Z-scheme Cu 2 O@Ag/g-C 3 N 4 NCs should thus have great potential for the integration of ultrasensitive SERS detection and efficient degradation of antibiotics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Xingyue Liu,Liucheng Meng,Wen Deng,Yuzhou Yang,Yanling Lou,Jianfeng Xi,Peng Li,Lei Zhang,Weibing Wu
Surface-enhanced Raman scattering (SERS) is a highly sensitive technology to detect target analytes. Herein, we propose a novel nanocellulose-based SERS platform integrating SERS with molecular imprinting technology (MIP) for ultrasensitive and selective detection of pesticide residues. A uniform, dense, and surface-clean “hot spots” nanostructure was eco-friendly constructed based on cellulose nanofiber (CNF) films. The CNF film serves as a substrate for the photo-induced in-situ growth of silver nanoparticles without additional reducing agents and surfactants. By regulating pre-polymer viscosity (8.7 mPa·s at 2-hour pre-polymerization), a thin and uniform MIP layer was precisely coated on the “hot spots” via dip-coating, ensuring optimal balance between selectivity and SERS activity. The resulting SERS-MIP architecture increases the likelihood that target molecules penetrate MIP-covered “hot spots”, yielding high sensitivity, reproducibility, and specificity toward parathion-methyl. The SERS substrate exhibited excellent sensitivity, reproducibility and selectivity for parathion-methyl, with a limit of detection of 3.97 × 10 −11 g/L and relative standard deviation of 7.60%. Moreover, it demonstrated remarkable capability in detecting pesticide residues on irregular surfaces of real samples, using a wiping sampling method that fully leverages the flexibility of the CNF film. This approach successfully integrates MIP with SERS technology, offering a flexible SERS substrate for on-site food safety monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Ruibo Zhao,Jingjing Bai,Qiang Zhang,Jiayi Wei,Fei Deng,Myunghee Kim,Xingjie Li,Shuli Man,Weipan Peng,Long Ma
Rapid detection of foodborne pathogens is critical for food safety. Conventional qPCR offers high sensitivity but relies on nucleic acid amplification, increasing operational complexity and false-positive risks. Here, we developed a biosensor platform named PAF-SERS ( P f Ago-mediated A mplification- F ree S urface- E nhanced R aman S cattering Biosensor) for detecting Salmonella typhimurium ( S. typhimurium ) without pre-amplification. In this platform, Pf Ago specifically cleaves target DNA guided by a single-stranded DNA guide, generating a linker sequence that enables quantitative detection through SERS signal conversion. The assay completes within 55 min, with a detection limit as low as 10 CFU/mL and a linear range of 10^ 1 to 10^ 7 CFU/mL, exhibiting high specificity and reproducibility. We further applied a support vector machine (SVM) algorithm to accurately classify low-concentration samples. The method was successfully validated in milk, eggs, chicken, and feces and blood from infected mice, demonstrating its potential for rapid, on-site detection without bulky instruments.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Liqian Niu,Xian Wang,Xinxin Liu,Xin Guan,Xinyu Fan,Xin Liu,Shiyue Niu,Shuyun Bi
A novel UiO-66/Leu/Au@AgNPs SERS substrate was fabricated for chlorthiamid detection. • A low LOD of 1.95 × 10 −9 mol L −1 and wide linear range were obtained for chlorthiamid. • The sensor showed good stability, repeatability, and high anti-interference ability. • Good recoveries in real samples confirmed its practical use in food safety analysis. A novel surface enhanced Raman scattering (SERS) substrate, UiO-66/Leu/Au@AgNPs, was fabricated by assembling gold@silver core-shell nanoparticles (Au@AgNPs) with leucine (Leu) and the zirconium-based metal-organic framework (MOF) (UiO-66). Various techniques including SEM, TEM, HAADF-STEM, XRD, XPS, FT-IR, TGA, UV–Vis, and Zeta potential were employed to fully characterize the substrate of UiO-66/Leu/Au@AgNPs. The substrate of UiO-66/Leu/Au@AgNPs achieved remarkable Raman signal amplification for chlorthiamid. Both electromagnetic enhancement (EM) and chemical enhancement (CM) mechanisms significantly boosted the distinct and reproducible Raman signal of chlorthiamid. The determination of chlorthiamide was not interfered by fourteen types of pesticides and several common metal ions, demonstrating the good selectivity of this method. The linear regression equation was expressed as I SERS = 128.08 c + 288,613.27 ( R 2 = 0.9982), The linear range spanned three orders of magnitude (from 3 × 10 −9 to 1 × 10 −6 mol L −1 ). A low limit of detection (LOD) of 1.95 × 10 −9 mol L −1 was obtained. The proposed method was further utilized for the determination of chlorthiamid in real samples, giving recoveries between 96.4% and 105% ( n = 5). Download: Download high-res image (118KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 3
·131
·
IF 5.4
Jin Meng,Ning Qiong,Zhang Mengping,Wei Haiyan,Meng Xiao,Chen Wenwen,Pan Hui,Xiu Haidi,Liu Lisheng,Wang Cuijuan
A novel surface-enhanced Raman scattering (SERS) based strategy is described for the rapid identification and quantitative determination of deltamethrin and its metabolite 3-phenoxybenzaldehyde in biological samples. A multifunctional sensor was developed using metal-organic framework-801 (MOF-801) and silver nanoflowers (Ag NFs), synthesizing an Ag NFs/MOF-801 composite. This composite is a dual-function material, acting as both a SERS substrate and an adsorbent, which was integrated into a portable 10-well plate. The addition of MOF-801 enhances enrichment selectivity and efficiency, while the abundant tips and rough surfaces of Ag NFs significantly improve SERS sensitivity. The determination of deltamethrin revealed a marked linear relationship between the SERS signal intensity and the logarithmic analyte concentration, with R-values falling within a range 0.959 to 0.972, with detection limits between 0.07 and 0.15 ppb, indicating suitability for clinical applications. This method successfully analyzed a patient sample, showing strong agreement with conventional chromatographic results while ensuring both practicality and accuracy. Notably, the detection process does not necessitate any sample preparation and can be completed within five min, highlighting its potential as an efficient and user-friendly POCD sensor for diagnosing poisoning in emergency settings.
Section
Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2026
·
IF 3.8
Liqian Niu,Xian Wang,Xinxin Liu,Xin Liu,Shiyue Niu,Bin Yang,Jia Liu,Shuyun Bi
A highly sensitive surface-enhanced Raman spectroscopy (SERS) method for the detection of acephate was established by using the substrate of silver nanoparticles (AgNPs) modified with N-acetyl-L-cysteine (NAC) and zinc oxide (ZnO). The Raman signal enhancement factor (EF) of acephate by the substrate of ZnO/NAC/AgNPs can be reached 4.95 × 10 6 . The ultraviolet-visible spectroscopy (UV-vis), high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), zeta potential, Fourier transform infrared spectra (FT-IR) and thermogravimetric analysis (TGA) were used to characterize the substrate of ZnO/NAC/AgNPs. The mechanism of electromagnetic enhancement (EM) assisted by chemical enhancement (CM) was elucidated in detail. The optimal experimental conditions were selected. The interference experiments showed that this method had high selectivity in complex substrates. There was a good linear relationship between acephate concentration and SERS signal intensity in the range of 6.00 × 10 −9 mol L −1 – 8.00 × 10 −7 mol L –1 , and the linear equation was I SERS = 85.02 c + 24712.49 ( r = 0.9982, c : nmol L −1 ) with the limit of detection (LOD) was 3.40 nmol L –1 (3 σ / S ). The SERS method was successfully applied for the detection of acephate residues in vegetable samples, and the recovery was in the range of 95.3% – 104% ( n = 5).
✓ 已验证
产品关联
DOI
高影响因子
Advanced Sustainable Systems
·
2022
·Vol. 7
·期 2
·2200387
·
IF 6.737
Meng He,Zhihao Li,Yujie Guo,Maofeng Zhang,Zhengdong Zhu,Xiaoyi Shi,Konghao Han,Youju Huang,Honglin Liu
The practical application of surface-enhanced Raman spectroscopy (SERS) technology for quantitative analysis still confronts the hinder of controlling the uniformity of signal and anchoring target molecules in a designed space. Herein, a facile method of devising the shrinkage of three-dimension (3D) hot spots droplets on a hydrophobic array substrate is demonstrated for the quantitative and multiplex detection of pesticide residues by using a portable Raman spectrometer. The shrunk droplet which is consisted of the analyte solution and AgNPs sol can induce simultaneously increasing density of plasmonic hot spots and immobilizing target molecules in hot spots. Besides, the closely packed Ag nanoparticles (AgNPs) are anchored in the gaps of the MoS 2 nanosheet array, generating uniform hotspots distribution with relative standard deviation (RSD) of about 8 %. In addition, the synergetic effects between MoS 2 and AgNPs further enhance the SERS sensitivity, and thereby target molecules of 10 −9 m are readily detected. Besides, the proposed strategy exhibits excellent SERS sensitivity for the analysis of pesticides down to 10 −8 m . Importantly, quantitative analysis and multi-components detection of pesticide residues on honeysuckle surface are achieved. Therefore, it is expected to be used for quantitative analysis of complex pesticide systems on real samples.
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Xiaoming Su,Xinyu Liu,Yangcenzi Xie,Mingyang Chen,Chao Zheng,Hong Zhong,Ming Li
Protein profiles of exosomes (EXOs) in clinical samples of cancer patients have become a promising diagnostic and therapeutic biomarker. However, simultaneous quantitative analysis of multiple exosomal proteins of interest remains challenging. To address the unmet need, we develop a paper-based surface-enhanced Raman spectroscopy (SERS)-vertical flow biosensor, named iREX (integrated Raman spectroscopic EXO) biosensor, for multiplexed quantitative profiling of exosomal proteins in clinical serum samples of patients. Utilizing this iREX biosensor, we are able to quantitatively profile MUC1, HER2 and CEA in EXO samples derived from various breast cancer cell subtypes. The results show discriminative expression profiles of the three exosomal proteins in these cell subtypes, which allows for accurate diagnosis and molecular subtyping of breast cancer. We further validate the clinical utility of the iREX biosensor for simultaneous quantitative analysis of MUC1, HER2 and CEA in patient’s blood serums, thereby aiding in noninvasive breast cancer subtyping and longitudinal treatment monitoring. Our iREX biosensor integrating the SERS detection in a vertical flow diagnostic device offers great advantages of high sensitivity, molecular specificity, powerful multiplexing capability, and high diagnostic accuracy. We believe that the iREX biosensor could be a promising clinical tool for comprehensive analysis of exosomal proteins in clinical samples for personalized diagnosis and precise management of breast cancer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2023
·Vol. 13
·期 2
·334
·
IF 5.3
Jie Li,Qiutian She,Wenxi Wang,Ru Liu,Ruiyun You,Yaling Wu,Jingzheng Weng,Yunzhen Liu,Yudong Lu
Label-free surface-enhanced Raman scattering (SERS) analysis shows tremendous potential for the early diagnosis and screening of colon cancer, owing to the advantage of being noninvasive and sensitive. As a clinical diagnostic tool, however, the reproducibility of analytical methods is a priority. Herein, we successfully fabricated Ag NPs/cellulose nanocrystals/graphene oxide (Ag NPs/CNC/GO) nanocomposite film as a uniform SERS active substrate for label-free SERS analysis of clinical serum. The Ag NPs/CNC/GO suspensions by self-assembling GO into CNC solution through in-situ reduction method. Furthermore, we spin-coated the prepared suspensions on the bacterial cellulose membrane (BCM) to form Ag NPs/CNC/GO nanocomposite film. The nanofilm showed excellent sensitivity (LOD = 30 nM) and uniformity (RSD = 14.2%) for Nile Blue A detection. With a proof-of-concept demonstration for the label-free analysis of serum, the nanofilm combined with the principal component analysis-linear discriminant analysis (PCA-LDA) model can be effectively employed for colon cancer screening. The results showed that our model had an overall prediction accuracy of 84.1% for colon cancer (n= 28) and the normal (n= 28), and the specificity and sensitivity were 89.3% and 71.4%, respectively. This study indicated that label-free serum SERS analysis based on Ag NPs/CNC/GO nanocomposite film combined with machine learning holds promise for the early diagnosis of colon cancer.Keywords:graphene oxide;label-free;PCA-LDA;colon cancer;SERS
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS RESEARCH
·
2022
·Vol. 38
·期 2
·519-531
·
IF 2.909
Zhu Sijia,Wang Qing,Yang Jinhui,Li Hongmei,Gao Ningning,Zhong Furu,Fu Jihong,Tang Jun,Wang Tao
Biomass-derived porous carbon (PC) loaded with precious metals have a synergistic enhancement effect on surface-enhanced Raman scattering (SERS). In this paper, PC containing various functional groups and large specific surface area, good stability and certain biocompatibility were prepared using tomato skins and introduced into the preparation of Ag nanoflowers (NFs) SERS substrates. Rhodamine 6G (R6G) was used as the Raman probe molecule to evaluate the sensing performance of Ag NFs@PC. The results showed that the stable dispersion and protective effect of PC on Ag NPs significantly improved the sensitivity and long-term stability of traditional Ag NFs. In practical application, Ag NFs@PC was further used to successfully achieve the quantitative analysis of trace methylene blue (10 ppt) and malachite green (10 ppt) in a lake water system. Therefore, SERS sensor Ag NFs@PC is expected to become a promising candidate sensor for detecting in the field of environment and food monitoring.
Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）