Cell Death Discovery
·
2023
·Vol. 9
·期 1
·1-15
·
IF 7
Lu Yang-yang,Zhu Chun-yang,Ding Yi-xin,Wang Bing,Zhao Shu-fen,Lv Jing,Chen Shu-ming,Wang Sha-sha,Wang Yan,Wang Rui,Qiu Wen-sheng,Qi Wei-wei
Cepharanthine (CEP), a bioactive compound derived from Stephania Cephalantha Hayata , is cytotoxic to various malignancies. However, the underlying mechanism of gastric cancer is unknown. CEP inhibited the cellular activity of gastric cancer AGS, HGC27 and MFC cell lines in this study. CEP-induced apoptosis reduced Bcl-2 expression and increased cleaved caspase 3, cleaved caspase 9, Bax, and Bad expression. CEP caused a G2 cell cycle arrest and reduced cyclin D1 and cyclin-dependent kinases 2 (CDK2) expression. Meanwhile, it increased oxidative stress, decreased mitochondrial membrane potential, and enhanced reactive oxygen species (ROS) accumulation in gastric cancer cell lines. Mechanistically, CEP inhibited Kelch-like ECH-associated protein (Keap1) expression while activating NF-E2 related factor 2 (Nrf2) nuclear translocations, increasing transcription of Nrf2 target genes quinone oxidoreductase 1 (NQO1), heme oxygenase 1 (HMOX1), and glutamate-cysteine ligase modifier subunit (GCLM). Furthermore, a combined analysis of targeted energy metabolism and RNA sequencing revealed that CEP could alter the levels of metabolic substances such as D (+) - Glucose, D-Fructose 6-phosphate, citric acid, succinic acid, and pyruvic acid, thereby altering energy metabolism in AGS cells. In addition, CEP significantly inhibited tumor growth in MFC BALB/c nude mice in vivo, consistent with the in vitro findings. Overall, CEP can induce oxidative stress by regulating Nrf2/Keap1 and alter energy metabolism, resulting in anti-gastric cancer effects. Our findings suggest a potential application of CEP in gastric cancer treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2023
·
IF 9.9
Yang Dongyue,Du Jiayi,Chen Kai,Zhang Haoran,Huang Gang,Liu Tong,Zhang Xinbo,Zhang Hongjie
Lithium-oxygen (Li-O 2 ) batteries have been regarded as an expectant successor for next-generation energy storage systems owing to their ultra-high theoretical energy density. However, the comprehensive properties of the commonly utilized organic salt electrolyte are still unsatisfactory, not to mention their expensive prices, which seriously hinders the practical production and application of Li-O 2 batteries. Herein, we have proposed a low-cost all-inorganic nitrate electrolyte (LiNO 3 −KNO 3 −DMSO) for Li-O 2 batteries. The inorganic nitrate electrolyte exhibits higher ionic conductivity and a wider electrochemical stability window than the organic salt electrolyte. The existence of K + can stabilize the O 2 − intermediate, promoting the discharge process through the solution pathway with an enlarged capacity. Even at an ultra-low concentration of 0.01 M, the K + can still remain stable to promote the solution discharge process and also possess a new function of inhibiting the dendrite growth by electrostatic shielding, further enhancing the battery stability and contributing to the long cycle lifetime. As a result, in the 0.99 M LiNO 3 −0.01 M KNO 3 −DMSO electrolyte, the Li-O 2 batteries exhibit prolonged cycling performance (108 cycles) and excellent rate performance (2 A·g −1 ), significantly superior to the organic salt one.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 36
·1-12
·
IF 2.8
Yang Huiyuan,Guo Rongshuo,Jin Linghua,Zhang Ye,Wang Weike
The design and fabrication of transition metal sulfides for supercapacitors have attracted considerable attention in recent years because of their potential for energy storage applications. Conventionally, the synthesis process involved subjecting them to high temperatures and pressures, which can be energy-intensive and environmentally unfriendly. Herein, microcrystalline NiCo 2 S 4 nanorods were prepared under ambient temperature and pressure using a two-step method. The Ni 1/3 Co 2/3 C 2 O 4 nanorods were initially synthesized using the coprecipitation technique, and then the NiCo 2 S 4 nanorods were obtained by sulfurating Ni 1/3 Co 2/3 C 2 O 4 with Na 2 S·9H 2 O under ambient conditions. The NiCo 2 S 4 as a high-performance supercapacitor electrode has been demonstrated. The results exhibited that the prepared NiCo 2 S 4 electrodes have low charge transfer resistance, and a high specific capacitance of 1330.0 F g −1 at a current density of 1.0 A g −1 . Furthermore, a hybrid supercapacitor was assembled using activated carbon (AC) as the anode material and NiCo 2 S 4 nanorods as the cathode material, and the resulting NiCo 2 S 4 //AC device exhibits an impressive energy density of 27.1 Wh kg −1 at a power density of 1.6 kW kg −1 . This work demonstrates a vision for fabricating a microcrystalline NiCo 2 S 4 cathode under ambient temperature and pressure for hybrid supercapacitors.
✓ 已验证
产品关联
DOI
高影响因子
Drug Delivery and Translational Research
·
2023
·
IF 5.4
Tang XiaoFei,Qin Huaiying,Zhang XiaoYun,Yang Haiyun,Yang Jianhua,Chen Ping,Jin Yinli,Yang Lu
Transdermal drug delivery systems (TDDS) have drawbacks such as poor absorption, low blood concentration, and delayed effects. Dissolving microneedle has sharp tips and short length, which overcome patients’ pain and improve transdermal efficiency but has low mechanical strength and drug loading capacity. This study thereby proposes a microemulsion-encapsulated and long-time-released transdermal microneedle (MN) delivery system with estradiol (Es) as the model drug. The microemulsion (ME) was optimized by utilizing the pseudo-ternary phase diagram and D-optimal mixture design. The estradiol microemulsion-encapsulated microneedle (Es-ME-MN) was optimized by Box–Behnken design and prepared by freeze–thaw method. The Es-ME-MN obtained was characterized and evaluated through a large variety of studies. Es-ME-MN had sufficient mechanical strength to pierce skin and was safe enough, the length of which was 600 μm, and the Es content was 177.12 ± 0.72 μg/patch without drug-excipient chemical interaction. In vitro permeation study showed that Es-ME-MN has a higher transdermal efficiency and lower retention capacity than commercial estradiol patch and conventional MN. Es plasma concentration began to increase at 3 h and remained at 12.98–23.52 ng/mL until 72 h by pharmacokinetic experiments in the Es-ME-MN group. Es-ME-MN rapidly achieves effective blood concentrations through needle puncture and microemulsion delivery and maintains blood concentrations through the baseplate long-time release. Microemulsion-encapsulated, organic solvent-free, and long-time-released transdermal microneedle will make progress and provide a new idea for transdermal delivery of lipophilic drugs. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiome
·
2023
·Vol. 11
·期 1
·1-15
·
IF 15.5
Fu Yao,Yao Songyang,Wang Tiankun,Lu Yongqiang,Han Huigang,Liu Xuening,Lv Dongying,Ma Xiao,Guan Shengyu,Yao Yujun,Liu Yunjie,Yu Haiying,Li Shengli,Yang Ning,Liu Guoshi
Background Methane (CH 4 ) is a major greenhouse gas, and ruminants are one of the sources of CH 4 which is produced by the rumen microbiota. Modification of the rumen microbiota compositions will impact the CH 4 production. In this study, the effects of melatonin on methane production in cows were investigated both in the in vitro and in vivo studies. Results Melatonin treatment significantly reduced methane production in both studies. The cows treated with melatonin reduced methane emission from their respiration by approximately 50%. The potential mechanisms are multiple. First, melatonin lowers the volatile fatty acids (VFAs) production in rumen and reduces the raw material for CH 4 synthesis. Second, melatonin not only reduces the abundance of Methanobacterium which are responsible for generating methane but also inhibits the populations of protozoa to break the symbiotic relationship between Methanobacterium and protozoa in rumen to further lowers the CH 4 production. The reduced VFA production is not associated with food intake, and it seems also not to jeopardize the nutritional status of the cows. This was reflected by the increased milk lipid and protein contents in melatonin treated compared to the control cows. It is likely that the energy used to synthesize methane is saved to compensate the reduced VFA production. Conclusion This study enlightens the potential mechanisms by which melatonin reduces rumen methane production in dairy cows. Considering the greenhouse effects of methane on global warming, these findings provide valuable information using different approaches to achieve low carbon dairy farming to reduce the methane emission.
Video Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2023
·Vol. 6
·期 5
·1-13
·
IF 20.1
Wang Ruijing,Jiang Lijuan,Wang Qunlong,Wei Guang-Feng,Wang Xuefeng
The design of efficient catalysts with abundant active sites is fascinating but still is a great challenge for hydrogen evolution reaction (HER). Herein, a highly active cobalt-embedded nitrogen-doped carbon nanotube hybrid array on nickel foam (Co-NCNT/NF) was constructed to enhance the HER activity. The 3D hydroxide nanosheets were utilized as a template and reactant to form the ordered ZIF-8 array by a solid–liquid interfacial release of Zn ions and coordination reaction, and the growth of ZIF-67 on the surface of ZIF-8 arrays assures the introduction of cobalt species into the precursor, catalyzing the formation of active cobalt-nitrogen-carbon (Co-N-C) sites. Based on the structure analysis and density functional theory calculations, an optimal configuration of the cobalt (111) face coated by carbon layer with one Co atom coordination to one N and C reveals a multi-site nature of the designed Co-NCNT/NF catalyst, which provided the improved conductivity, 3D hierarchical structure, the uniform distribution, and efficient exposure of active Co–N-C sites. A small overpotential of 157 mV at a current density of − 10 mA cm −2 for HER in 1 M KOH was obtained, proving that the optimized proton adsorption on the multi-sites structure promotes the HER activity of Co-NCNT/NF.
✓ 已验证
产品关联
DOI
高影响因子
Nano Research
·
2023
·Vol. 16
·期 8
·11250-11258
·
IF 9.9
Zhang Yunqin,Cao Yang,Liu Yan,Yang Yingjie,Chen Mingmao,Gao Hang,Lin Lushan,Chen Xueyuan
Currently, enzyme-responsive nanomaterials have shown great promise in prognosis or diagnosis of disease biomarker. However, the great obstacle for conventional enzyme-responsive nanomaterials frequently lies in autofluorescence interference, poor monodispersity, uncontrollable size and morphology, low optical stability, and biotoxicity, which fundamentally impede their practical application in biological systems. To overcome these deficiencies, we proposed a novel strategy for reliable and precise detection of an enzyme disease biomarker, alkaline phosphatase (ALP), through lanthanide (Ln 3+ ) nucleotide nanoparticles (LNNPs) with extremely improved monodispersity and uniformity, which were achieved by the coordination self-assembly between ATP and Ln 3+ inside micellar nanoreactor. Specifically, for ATP-Ce/Tb LNNPs, highly improved photoluminescence (PL) emission of Tb 3+ can be achieved via efficient Ce 3+ sensitization. We demonstrated that ALP could specifically cleave the phosphorus–oxygen (P–O) bonds of ATP and result in the collapse of ATP-Ce/Tb scaffold, finally leading to the PL quenching of Tb 3+ . By taking advantage of time-resolved (TR) PL technique, the fabricated ATP-Ce/Tb LNNPs presented superior selectivity and sensitivity for the ALP bioassay in complicated serum samples, thus revealing the great potential of ATP-Ce/Tb LNNPs in the areas of ALP-related disease prognosis and diagnosis.
✓ 已验证
产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2023
·
IF 3.8
Jia Dong-Xu,Yu Hai,Wang Fan,Jin Li-Qun,Liu Zhi-Qiang,Zheng Yu-Guo
Cellobiose 2-epimerase (CE) is ideally suited to synthesize lactulose from lactose, but the poor thermostability and catalytic efficiency restrict enzymatic application. Herein, a non-characterized CE originating from Caldicellulosiruptor morganii (CmCE) was discovered in the NCBI database. Then, a smart mutation library was constructed based on FoldX ΔΔG calculation and modeling structure analysis, from which a positive mutant D226G located within the α 8 /α 9 loop exhibited longer half-lives at 65–75 °C as well as lower K m and higher k cat / K m values compared with CmCE. Molecular modeling demonstrated that the improvement of D226G was largely attributed to the rigidification of the flexible loop, the compactness of the catalysis pocket and the increment of substrate-binding capability. Finally, the yield of synthesizing lactulose catalyzed by D226G reached 45.5%, higher than the 35.9% achieved with CmCE. The disclosed effect of the flexible loop on enzymatic stability and catalysis provides insight to redesign efficient CEs to biosynthesize lactulose. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Reproductive Sciences
·
2023
·
IF 2.9
Shao Mingkun,Hou Mengyao,Li Sainan,Qi Wenjin
Vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC) are the most common lower genital tract infections in reproductive women. In recent years, the research on its pathogenesis mainly focuses on vaginal local immunity and IL-17 as key factors in adaptive immunity, attracting much attention. However, the role of IL-17 in local immunity in VVC and RVVC is poorly understood. At the same time, neutrophils are considered the most effective way to control and eliminate candidal infection and have a controversial role in VVC and RVVC. In this study, we built a mouse RVVC model. After analyzing the vaginal lavage solution of RVVC mice with an inflammatory factor antibody chip and ELISA, we found that IL-17 may play a protective role in RVVC. The experiment of constructing RVVC mice with different concentrations of IL-17 using halofuginone and comparing the vaginal fungi load and vaginal epithelial damage verified that IL-17 had a protective effect in RVVC. In addition, in vitro and in vivo studies found that IL-17 can promote neutrophil apoptosis and recruit neutrophils in the vagina. The neutrophils in the vagina can secrete IL-17 in an autocrine manner. These two may be why IL-17 plays a protective role in RVVC. In summary, the study suggests that IL-17-mediated regulation of neutrophil function is involved in host immune response to RVVC, which helps us to further understand the potential mechanism of IL-17 in RVVC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 4
·673-679
·
IF 3.1
Wang Teng,Hu Renquan,Wei Hao,Wei Zehui,Yan Meng,Yang Yong
Developing low-cost Ni-based amorphous/crystalline composites with well-defined nanostructures is expected to achieve a highly efficient oxygen evolution reaction(OER) by exposing more active sites and enhancing the electrical conductivity, but it still remains a synthetic challenge. Here, a crystalline/amorphous composite composed of crystalline Ni 3 Se 4 and amorphous Ni hydroxide with a multi-layered bowl-shaped nanostructure was synthesized by a simple one-step solvothermal method. By regulating the concentration of sulfate ions in the reaction solution, the single-layered nanosheets achieve a transformation into a multi-layered structure with more exposed active sites. In addition, the crystalline-amorphous heterostructure allows regulation of the interfacial electronic structures, and the decoration of Ni 3 Se 4 can effectively enhance the electrical conductivity of composites. Benefiting from the interfacial synergy between Ni 3 Se 4 and Ni hydroxide, the as-optimized Ni 3 Se 4 /Ni hydroxide as an OER catalyst displayed superior electrocatalytic activity with a low overpotential of 285 mV at a current density of 10 mA/cm 2 , a small Tafel slope of 68.3 mV/dec and remarkable stability in alkaline solution. This work offers a novel and effective method for the design of functional crystalline/amorphous composites for energy conversion and storage.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 9
·1-10
·
IF 5.7
Fredj Zina,Wang Pengbo,Ullah Fateh,Sawan Mohamad
A novel aptasensor has been designed for quantitative monitoring of epinephrine (EP) based on cerium metal–organic framework (CeMOF) loaded gold nanoparticles (AuNPs). The aptamer, specific to EP, is immobilized on a flexible screen-printed electrode modified with AuNPs@CeMOF, enabling highly selective binding to the target biomolecule. Under optimized operational conditions, the peak currents using voltammetric detection measured at voltage of 83 mV (vs. Ag/AgCl) for epinephrine exhibit a linear increase within concentration in the range 1 pM–10 nM. Following this detection strategy, a boasted limit of detection of 0.3 pM was achieved, surpassing the sensitivity of most reported methods. The developed biosensor showcased exceptional performance in detection of EP in spiked serum sample, with remarkable recovery range of 95.8–113% and precision reflected by low relative standard deviation (RSD) ranging from 2.23 to 6.19%. These results indicate the potential utility of this biosensor as a valuable clinical diagnostic tool. Furthermore, in vitro experiments were carried out using the presented biosensor to monitor the release of epinephrine from PC12 cells upon extracellular stimulation with K + ions. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2023
·
IF 2.8
Wang Jing,Chen Hao,Jiao Chaonan,Dai Jian,Peng Yinxian,Ding Jian
The binary metal oxide-based catalysts have been proven to exhibit superior catalytic performances than their single metal oxides. As active supports, the physical and chemical properties of metal oxides would influence the chemical and electronic interactions with noble metal nanoparticles. By precise control of the composition and structure of metal oxides, the synergistic interaction between noble metals and transition metal oxides is expected to be realized. Herein, a novel hierarchical structured mCeO 2 NR/Mn 3 O 4 /Pt catalysts were designed for low-temperature toluene combustion. The mCeO 2 NR/Mn 3 O 4 composites were fabricated by a simple and effective two-step sequential hydrothermal synthesis approach. After subsequent loading of Pt nanoparticles over mCeO 2 NR/Mn 3 O 4 by impregnation and reduction methods, the mCeO 2 NR/Mn 3 O 4 /Pt catalysts with hierarchical structure were successively synthesized. The catalytical performances of the catalyst were evaluated by the toluene oxidation reaction. It was observed that the catalytic activities of the catalysts were closely related with the contents of Pt and Mn 3 O 4 , and mCeO 2 NR/Mn 3 O 4 /Pt with 2.1% Mn and 0.45% Pt exhibited the greatest catalytic activity ( T 95 = 165 °C) toward the oxidation of toluene. The apparent activation energy for toluene oxidation with mCeO 2 NR/Mn 3 O 4 /Pt (2.1% Mn) was as low as 35.3 kJ/mol. The improvement of the catalytical performance was due to the synergistic effect between CeO 2 and Mn 3 O 4 that led to the increase of oxygen vacancies and the enhancement of redox capacity, and the good dispersion of Pt active sites over the mCeO 2 NR/Mn 3 O 4 . Graphical Abstract
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）