FOOD CHEMISTRY
·
2022
·
IF 9.231
Wei Jia,Chenna Di,Rong Zhang,Lin Shi
Honey aroma is one of the most significant factors of Feng-flavor Baijiu, which is also an essential element to attract consumers. However, the evaluation and chemical basis of honey aroma is unclear. Palmitoleic acid, lagochilin, phomotenone and ethyl behenate were confirmed to be the strongest contributors to honey aroma by time-intensity analysis and UHPLC-Q-Orbitrap-MS. Predictive modeling was developed for processing honey aroma intensity responses in order to obtain significant Feng-flavor Baijiu rankings. In this study, the effects of ex-vivo saliva on Feng-flavor Baijiu were investigated for the first time. Mucin and α-amylase, as major proteins in ex-vivo saliva, were applied to simulate molecular docking of ethyl benzoate. Mucin and α-amylase modified the aroma release, which depended on hydrogen bonds and hydrophobic interactions, respectively. It is blazing a trail in the field in sensory experience of Feng-flavor Baijiu as well as contributes to our understanding of Feng-flavor Baijiu drinking process.
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高影响因子
CELL CYCLE
·
2022
·
IF 5.173
Feng Zhang,Peng Liu,Zhaopeng He,like Zhang,Xinqi He,Feng Liu,Jinsheng Qi
Crocin (CRO) is feasible in alleviating atherosclerosis (AS), the mechanism of which was therefore explored in the study. High-fat diet (HFD)-induced apolipoprotein E-deficient (ApoE−/−) mice and lysophosphatidic acid (LPA)-treated macrophages received CRO treatment. Treated macrophage viability was determined via MTT assay. In both murine and macrophage, the lipid level and total Cholesterol/Cholesteryl l Ester (TC/CE) levels were quantified by oil-red-O staining and ELISA, respectively. Lipid droplet, aortic plaque formation and collagen deposition were detected via Oil-red-O staining, hematoxylin–eosin staining and Masson staining, respectively. Liver X Receptor-α (LXR-α), Peroxisome Proliferator-Activated Receptor γ (PPARγ), CD68, PCSK9, CD36, ATP Binding Cassette Subfamily A Member 1 (ABCA1), phosphorylated (p)-AKT, and AKT expressions were detected via Western blot, the former three also being detected using Immunohistochemistry and the first being measured by qRT-PCR. CRO decreased HFD-induced weight gain, ameliorated the abnormal serum lipid levels of HFD-treated mice, and inhibited aortic plaque formation and lipid deposition, and increased collagen fibers, with upregulated high-density lipoprotein-cholesterol (HDL-C) and downregulated TC and low-density lipoprotein-cholesterol (LDL-C). CRO alleviated the HFD-induced upregulations of CD68, PCSK9 and CD36 as well as downregulations of PPARγ/LXR-α, ABCA1 and AKT phosphorylation. In LPA-treated macrophages, CRO alone exerted no effect on the viability yet inhibited the lipid droplets formation and downregulated TC/CE levels. Silent LXR-α reversed the effect of CRO on the lipid droplets formation and levels of lipid metabolism-related factors. CRO ameliorated AS by inhibiting foam cells formation and promoting reverse cholesterol transport via PPARγ/LXR-α.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 10
·4170
·
IF 4.6
Jing Chen,Qi Huang,Zhuobin He,Guoying Tan,Yuansheng Zou,Juying Xie,Zhengming Qian
Polygoni Cuspidati Rhizoma et Radix (PCR), the rhizome and root ofPolygonum cuspidatumSieb. et Zucc., has been used as an herbal medicine for a long time. In this study, the ultrafiltration combined with high performance liquid chromatography (UF-HPLC) method was developed to screen tyrosinase (TYR), α-glucosidase (α-GLU), and xanthine oxidase (XOD) inhibitors from PCR. Firstly, the inhibitory activity of 50% methanol PCR extract on TYR, α-GLU, XOD, and acetylcholinesterase (ACHE) was tested. The extract showed a good inhibition on the enzymes, except for ACHE. Therefore, UF-HPLC experiments were carried out to screen TYR, α-GLU, and XOD inhibitors from PCR extract. Seven potential bioactive components were discovered, including methylgallate (1), 1,6-di-O-galloyl-D-glucose (2), polydatin-4′-O-D-glucoside (3), resveratrol-4′-O-D-glucoside (4), polydatin (5), malonyl glucoside resveratrol (6), and resveratrol-5-O-D-glucoside (7). Most of them were found as enzyme inhibitors from PCR for the first time, except polydatin (5), which had been reported as an α-GLUI in PCR in the literature. Finally, molecular docking analysis was applied to validate the interactions of these seven potential active components with the enzymes. Compounds1–7were proven as TYR inhibitors, compounds2,4–7were identified as XOD inhibitors, and compounds4–6were confirmed as α-GLU inhibitors. In short, the current study provides a good reference for the screening of enzyme inhibitors through UF-HPLC, and provides scientific data for future studies of PCR.Keywords:Polygoni Cuspidati Rhizoma et Radix;enzyme inhibitor;tyrosinase;xanthine oxidase;α-glucosidase;ultrafiltration
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Nano Research
·
2023
·Vol. 16
·期 7
·10026-10033
·
IF 9.9
Jiang Shihui,Ke Jianxi,Li Guowei,Liu Aijun,Li Xiaohan,Ye Lixiang,Jiang Feilong,Liu Yongsheng,Hong Maochun
Hexagonal-phase NaYF 4 (β-NaYF 4 ) has been acknowledged to be one of the most efficient doping hosts to prepare bright lanthanide-doped luminescent nano-bioprobes for various biomedical applications. However, to date, it remains a great challenge to synthesize ultra-bright lanthanide-doped β-NaYF 4 nano-bioprobes under a low reaction temperature by using conventional synthetic methods. Herein, we first develop an acetic acid (HAc)-mediated coprecipitation method for the preparation of ultra-bright lanthanide-doped β-NaYF 4 nanoprobes under a low reaction temperature at 200 °C. Based on a series of comparative spectroscopic investigations, we show that the use of HAc in the reaction environment can not only promote the rapid α–β phase transformation of NaYF 4 host at 200 °C within 1 h but also boost the absolute photoluminescence quantum yield (PLQY) of NaYF 4 nanocrystals to 30.68% for near-infrared emission and to 3.79% for upconversion luminescence, both of which are amongst the highest values for diverse lanthanide-doped luminescent nanocrystals ever reported. By virtue of their superior near-infrared luminescence, we achieve optical-guided dynamic vasculature imaging in vivo of the whole body at a high spatial resolution (23.8 µm) under 980 nm excitation, indicating its potential for the diagnosis and treatment evaluation of vasculature-related diseases.
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DALTON TRANSACTIONS
·
2021
·Vol. 50
·期 48
·18061-18068
·
IF 4.39
Jiahui Guo,Xueqing Feng,Songrui Wang,Qikang Wu,Shanshan Lv,Yan Zhou,Han Li,Zheng Chen,Yuzhong Zhang
Novel hexagonal α-cobalt hydroxide nanosheets are synthesized through a 2-methylimidazole-induced hydrolysis strategy with cetyltrimethylammonium bromide (CTAB) as a surfactant. The weak alkaline environment provides favorable conditions for the formation of metastable α-Co(OH)2, while the same raw material will produce β-Co(OH)2 when a strong alkali solution is used. CTAB plays a vital role not only in hexagonal oriented growth, but also in the formation of the hydrotalcite-like structure of α-Co(OH)2 with high crystallinity. The crystallinity of both α- and β-Co(OH)2 is very poor without CTAB as a surfactant. The Co in this Co(OH)2−x layer presents most of the CoII and a small part of the CoIII, and the interlayer nitrate anion balances the positive charge of the host layer. The redox function produced by the CoII and CoIII of α-Co(OH)2 together with the large layer spacing jointly promotes the electron and mass transfer. The use of hydrazine hydrate for transfer hydrogenation involves the transport of protons and electrons produced by decomposition, and the rapid transport is bound to be conducive to the reduction process. Nitro compounds with varieties of functional groups can be smoothly reduced to the corresponding amines with high selectivity, when α-Co(OH)2 was used as a catalyst under mild conditions.
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Food Bioscience
·
2021
·
IF 4.24
Jia-Bin Wang,Le-Yi Yu,Xu Zeng,Jun-Wei Zheng,Bin Wang,Li Pan
Inhibition of α-glucosidase activity is an important strategy in lowering the concentration of blood sugar . In this paper, using domestic and foreign characteristic food-derived substances as the sources of lactobacillus , the performance of them were evaluated by measuring the strains’ α-glucosidase inhibitory ability. Finally, the cell-free extracellular supernatants (CFS) of Lactobacillus rhamnosus LB1lac10 was determined to have the highest α-glucosidase inhibition ability. Based on the Nanopore third-generation sequencing technology platform, the genome of LB1lac10 was sequenced and functional gene annotation was performed. After that, the biological activity and structural composition of the exopolysaccharide produced by L. rhamnosus LB1lac10 were studied. The purified exopolysaccharide EPS1-1 also showed efficient α-glucosidase inhibitory ability. The structure and conformation characteristics of EPS1-1 were further analyzed. The EPS1-1 from L. rhamnosus LB1lac10 had a molecular weight of 88,650 Da, and it was mainly composed of mannose , glucuronic acid , glucose, xylose , galactose , and arabinose . From the FT-IR and NMR analyses, EPS1-1 had functional groups of a typical polysaccharide structure and contained two types of glycosidic bonds with α-configuration pyranose . The main glycosidic bond corresponded to → 4)-α- d -Glcp-(1→, which might be an important reason why EPS1-1 could inhibit α-glycosidase. Thermodynamic studies showed that EPS1-1 had high heat resistance to meet the needs of food processing. The results suggested that L. rhamnosus LB1lac10 could be used as a potential probiotic to lower blood sugar, and the EPS1-1 has the potential to serve as a natural α-glycosidase inhibitor to regulate the concentration of blood glucose.
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AAPS PHARMSCITECH
·
2021
·Vol. 23
·期 1
·1-11
·
IF 3.246
Liu Hongfei,Zou Yi,Zhu Jie,He Haibing,Feng Yingshu,Firempong Caleb Kesse,Yu Yang,Sun Changshan
Recombinant human interferon α-2b (rhINF-α-2b), like most proteins, has several shortcomings such as relatively short half-life, low therapeutic index, high circulating drug fluctuations, and rapid degradation which could hinder its effective delivery. Novel electrostatic spray and ion exchange drug-loading techniques were combined to formulate rhINF-α-2b sodium hyaluronate cross-linked porous sustained-release microspheres (rhINF-α-2b-SHCPM), a protein delivery system. The different properties of rhINF-α-2b-SHCPM including the physicochemical nature, in vitro release behavior, and antitumor activity were evaluated. The loading rate (10.31 ± 0.94%) and encapsulation efficiency (89.09 ± 2.37%) of rhINF-α-2b-SHCPM produced acceptable values. The in vitro cumulative release rate of rhINF-α-2b-SHCPM within 24 h was also 86.26 ± 2.11% with a much better sustained release effect. Thus, the half-life (10.763 h) and retention time (14.067 h) of rhIFNα-2b-SHCPM were significantly prolonged with enhanced bioavailability (43,198.387 ng/L*h) and decreased peak concentration (15,266.4 ngL -1 ) compared with the free rhIFNα-2b protein (0.912 h, 0.952 h, 34,749.048 ng/L*h, and 48,870.2 ngL -1 , respectively). The in vitro anti-proliferative activity and in vivo tumor inhibitory rate of rhIFNα-2b-SHCPM also increased by 90 and 55.86%, respectively, compared with the free rhIFNα-2b solution. The findings significantly supported a well-developed protein delivery system with improved sustained release, acceptable bioavailability, and increased antitumor activities.
Graphical Abstract
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2021
·
IF 2.262
Xiaojuan Wang,Hanqing Gu,Guolin Wu
A series of poly (amino acid) with zwitterionic character was prepared for surface coating to avoid protein adsorption, by modifying the poly (aspartic acid) side chain with tertiary amine groups. An interesting and facile procedure is described with starts with polysuccinic acid which is followed by ring-opening with amino functions and finally hydrolysis of the remaining succinic acid groups achieving the polyaspartic acid structure as backbone. The degree of cationic tertiary amino group was easily varied by the degree of ring-opening with amines. The materials show isoelectric point between pH 4 and 9, have a typical zwitterionic character, and can be used to coat negatively as well as positively charged surfaces, depending on composition. The protein adsorption behavior is thus pH dependent and was studied with various proteins mainly available in blood. The adsorption test proved that a significant reduction of protein adsorption could be found for fibrinogen and albumin, and this protein-repellent behavior is strongly dependent on the amount of the absorbed polymer (adsorbed at pH = 7.4). This is a nice additional contribution to polymers with zwitterionic character used as coating reducing nonspecific protein adsorption.
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高影响因子
Nanoscale
·
2021
·Vol. 13
·期 47
·20170-20185
·
IF 7.79
Yanqin Song,Muhammad Ismail,Qi Shan,Jianing Zhao,Yanping Zhu,Leiming Zhang,Yuan Du,Longbing Ling
Rheumatoid arthritis (RA) is an autoimmune inflammatory disorder that has seriously affected human health worldwide and its current management requires more successful therapeutic approaches. The combination of nanomedicines and pathophysiology into one system may provide an alternative strategy for precise RA treatment. In this work, a practical ROS-mediated liposome, abbreviated as Dex@FA-ROS-Lips that comprised synthetic dimeric thioether lipids (di-S-PC) and a surface functionalized with folic acid (FA), was proposed for dexamethasone (Dex) delivery. Incorporation with thioether lipids and a FA segment significantly improved the triggered release and improved the triggered release of cytotoxic Dex as well as the active targeting of RA, altering its overall pharmacokinetics and safety profiles in vivo. As proof, the designed Dex@FA-ROS-Lips demonstrated effective internalization by LPS-activated Raw264.7 macrophages with FA receptor overexpression and released Dex at the inflammatory site due to the ROS-triggered disassembly. Intravenous injection of this Dex@FA-ROS-Lips into adjuvant-induced arthritis (AIA) mice led to its incremental accumulation in inflamed joint tissues and significantly alleviated the cartilage destruction and joint swelling via suppression of proinflammatory cytokines (iRhom2, TNF-α and BAFF), as compared to the effect of commercial free Dex. Importantly, the Dex@FA-ROS-Lips nanoformulation showed better hemocompatibility with less adverse effects on the body weight and immune organ index of AIA mice. The anti-inflammatory mechanism of Dex@FA-ROS-Lips was further studied and it was found that it is possibly associated with the down-regulation of iRhom2 and the activation of the TNF-α/BAFF signaling pathway. Therefore, the integration of nanomedicines and the RA microenvironment using multifunctional Dex@FA-ROS-Lips shall be a novel RA treatment modality with full clinical potential, and based on the enhanced therapeutic effect, the signaling pathway of iRhom2/TNF-α/BAFF reasonably explained the mechanism of Dex@FA-ROS-Lips in anti-RA, which suggested a molecular target for RA therapy and other inflammatory diseases.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Qun Yu,Yuhang Yang,Yan Xu,Yuqiao Wang,Qingdong Qin
The application of metal doping has emerged as a prominent approach for modifying transition-metal-based heterogeneous catalysts in activating peroxymonosulfate (PMS). Herein, silver-doped α-MnO 2 (Ag-α-MnO 2 ) was synthesized via a one-step hydrothermal approach and utilized for activating PMS to degrade sulfamethoxazole (SMX). The catalyst was systematically characterized and examined to determine that the efficient dispersion of Ag increased the specific surface area and pore volume of α-MnO 2 . The degradation experiments demonstrated that SMX could be efficiently degraded by a combination of PMS and Ag-α-MnO 2 within 60 min. The apparent kinetic rate constant was 0.039 min −1 , which was approximately ten times higher than that of α-MnO 2 . The impacts of external water conditions on catalytic performance and the stability of Ag-α-MnO 2 were subsequently investigated. Density functional theory calculations revealed that Ag as a crucial active center reduced the PMS adsorption energy, promoted electron transfer, and orchestrated electron distribution. EPR spectra, quenching experiments, electrochemical tests, and XPS analysis were performed to validate the radical and non-radical mechanisms, including SO 4 − , OH, O 2 − and 1 O 2 . The two redox cycles of Ag + /Ag 0 and Mn 4+ /Mn 3+ on the surface of Ag-α-MnO 2 contributed to the activation of PMS. Additionally, probable degradation pathways for SMX were presented. This study provides an initially valuable insight into the cooperative mechanism of PMS activation for SMX degradation employing Mn-based catalysts doped with precious metal silver.
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LUMINESCENCE
·
2023
·
IF 2.9
Huanzong Zhang,Qingshui Wang,Fan Cai,Chaoqun Huang,Yinghui Wang,Junneng Zhang,Jinxu Huang
Rapid and accurate identification of tumor metabolic markers is important for early tumor diagnosis and individualized treatment. Here, a stable monodisperse sub-nanometer platinum (Pt) material was developed as a highly efficient nanozyme with a specific activity of peroxidase as high as 20.86 U mg −1 through the growth of in situ domain-limited Pt quantum dots via the polymer polyvinylpyrrolidone. Further, the synthesis of large quantities of Pt-loaded SiO 2 (Pt-SiO 2 ) was determined by silylation reaction and used for naked eye colorimetric testing of human alpha-fetoprotein (AFP). In particular, the immunization incubation process occurred in preprepared microplates. A nanozyme-based immunomodel was constructed in the presence of the target AFP, and a chromogenic reaction occurred with exogenous hydrogen peroxide and the chromogenic substrate tetramethylbenzidine. On optimization of experimental conditions, the dynamic working response range for AFP was found to be 0.05–20 ng mL −1 , with a limit of detection of 38.7 pg mL −1 . This work provides a new strategy to design efficient nanozyme-based enzyme-linked immunochromatographic platforms to meet the practical use of replacing natural enzymes.
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高影响因子
ADVANCED MATERIALS
·
2023
·
IF 29.4
Mingyuan Wang,Qiong Huang,Min Liu,Tianjiao Zhao,Xiangping Song,Qiaohui Chen,Yongqi Yang,Yayun Nan,Zerun Liu,Yuntao Zhang,Wei Wu,Kelong Ai
The increased incidence of inflammatory bowel disease (IBD) has seriously affected the life quality of patients. IBD develops due to excessive intestinal epithelial cell (IEC) apoptosis, disrupting the gut barrier, colonizing harmful bacteria, and initiating persistent inflammation. The current therapeutic approaches that reduce inflammation are limited. Although IBD can be treated significantly by directly preventing IEC apoptosis, achieving this therapeutic approach remains challenging. Accordingly, we are the first to develop an oral pifithrin-α (PFTα, a highly specific p53 inhibitor) embedded nanomedicine (OPEN) to effectively treat IBD by inhibiting excessive IEC apoptosis. As a major hub for various stressors, p53 is a central determinant of cell fate, and its inhibition can effectively reduce excessive IEC apoptosis. The tailored OPEN can precisely inhibit the off-target and inactivation resulting from PFTα entry into the bloodstream. Subsequently, it persistently targets IBD lesions with high specificity to inhibit the pathological events caused by excessive IEC apoptosis. Eventually, OPEN exerts a significant curative effect compared with the clinical first-line drugs 5-aminosalicylic acid (5-ASA) and dexamethasone (DEX). Consequently, the OPEN therapeutic strategy provides new insights into comprehensive IBD therapy. This article is protected by copyright. All rights reserved
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