ULTRASONICS SONOCHEMISTRY
·
2020
·
IF 6.513
Subramanian Siva,Changzhu Li,Haiying Cui,Venkatasamy Meenatchi,Lin Lin
Essential oils derived from medicinal plants are prosperous sources of active components having high biological potential. Cuminaldehye and isoeugenol, are hydrophobic essential oil components (EOC), are showing drastic limitations in their applications by low water solubility and the respective volatility. Methyl- β -cyclodextrin inclusion complexes (M β CD-ICs) were prepared in aqueous solution and in solid state with the EOC via the ultrasonication method, an energy saving, high efficiency and eco-friend technique, aim to extend their aqueous solubility and biological properties. UV–Vis absorption, fluorescence, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), proton nuclear magnetic resonance ( 1 H NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, powder X-ray diffraction (PXRD) and computational simulations confirmed the formation of EOC/M β CD-ICs. Result of solubility studies proved the enhanced solubilization of EOC in the presence of M β CD in aqueous and double reciprocal profiles substantiated the guest/host stoichiometry of 1:1. TGA and DSC studies indicated the improved stability of EOC in M β CD-ICs. The efficiency of ICs in terms of the antioxidant activity was verified and the IC displayed higher antioxidant activity compared to that of free EOC, as determined by free radical scavenging assay. Finally, the antibacterial effect of EOC/M β CD-ICs against gram-positive Staphylococcus aureus and gram-negative Escherichia coli bacteria was demonstrated. Overall results not only revealed the potential of M β CD on the bioavailability, solubility and stability, but also that the intensification caused by the IC may be greater that the antioxidant and antibacterial effects of the selected EOC for this study.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 11
·2551-2564
·
IF 3.637
Yuan Meng-Meng,Zou Jiao,Huang Zhao-Ning,Peng Dong-Ming,Yu Jin-Gang
A novel electrochemical sensor, platinum nanoparticles/graphene nanoplatelets/multi-walled carbon nanotubes/β-cyclodextrin composite (PtNPs-GNPs-MWCNTs-β-CD) modified carbon glass electrode (GCE), was fabricated and used for the sensitive detection of folic acid (FA). The PtNPs-GNPs-MWCNTs-β-CD nanocomposite was easily prepared with an ultrasound-assisted assembly method, and it was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The electrochemical behavior of FA at PtNPs-GNPs-MWCNTs-β-CD/GCE was investigated in detail. Some key experimental parameters such as pH, amount of PtNPs-GNPs-MWCNTs-β-CD composite, and scan rate were optimized. A good linear relationship ( R 2 = 0.9942) between peak current of cyclic voltammetry (CV) and FA concentration in the range 0.02–0.50 mmol L −1 was observed at PtNPs-GNPs-MWCNTs-β-CD/GCE. The detection limit was 0.48 μmol L −1 (signal-to-noise ratio = 3). A recovery of 97.55–102.96% was obtained for the determination of FA in FA pills (containing 0.4 mg FA per pill) at PtNPs-GNPs-MWCNTs-β-CD/GCE, indicating that the modified electrode possessed relatively high sensitivity and stability for the determination of FA in real samples. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 6
·2331-2338
·
IF 3.978
Shushu Ding,Yunxia Xu,Qi Liu,Hui Gu,Anwei Zhu,Guoyue Shi
Ultrasensitive detection of monomeric β-amyloid peptides is of fundamental significance for studying the pathological progression of Alzheimer's disease (AD). In this article, by facilely engineering a gold microelectrode interface, we developed a novel electrochemical biosensor for sensitive and selective monitoring of β-amyloid peptide (Aβ) monomers in cerebrospinal fluid (CSF). Through specific Cu2+-Aβ-hemin coordination, Aβ directed the assembly of Cu2+-PEI/AuNPs-hemin nanoprobes into network aggregates on a microelectrode interface, which promoted the enrichment of Aβ monomers on the microelectrode. Furthermore, the AuNP aggregate promotes the deposition of silver nanoparticles, which were utilized for the electrochemical stripping analysis of the Aβ monomer. The proposed method displayed ultra-sensitivity for Aβ monomers with the detection limit down to 0.2 pM. Besides, high selectivity toward Aβ monomers was observed. These remarkable analytical performances render the electrochemical biosensor useful for evaluating the dynamic change of Aβ monomer level in CSF of live mice with AD, promoting the investigation of the role that Aβ monomers play in brain events.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Dong Ji,Zhen Wang,Yanjiao Zhu,Haibin Yu
Hexagonal boron nitride nanosheets (BNNs) are gaining much attention owing to their extraordinary performance; however, difficulties remain in achieving their large-scale preparation and dispersion at high concentration in solvents. A facile yet efficient β-cyclodextrin-assisted mechanochemical exfoliation method is proposed to exfoliate and functionalize hexagonal boron nitride (h-BN). The β-cyclodextrin-assisted ball milling process gave a high yield of 60%, with the resultant BNNs being covalently grafted with hydroxyl, and well dispersed in water and other solvents. The concentration of the hydroxyl-functionalized boron nitride nanosheets (OH-BNNs) reached 3 mg/mL, and was stable for several weeks. Next, we fabricated a membrane based on the resulting OH-BNNs to effectively capture Congo red dye. This work not only offers an environmentally friendly, efficient, solvent-less and extremely cost-effective scalable production method, but also assists in our understanding of the mechanism of exfoliation and functionalization in other layered materials.
✓ 已验证
产品关联
DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2020
·
IF 3.333
Qikun Wei,Lan Bai,Xiaomei Qin,Chunyan Hu,Lei Li,Wei Jiang,Fei Song,Yuzhong Wang
The adsorption capacity of existing β-CD-based adsorbents is generally restrained by the limited dimension of β-CD's inherent cavity. To find an effective way to expand the inclusion/adsorption capacity of β-CD, a series of N-containing groups, amine with straight chain, imidazole with rigid five-membered ring and pyridine with rigid six-membered ring, are firstly utilized to alkylate the secondary rim of β-CD through the thiol-Michael addition. The capsulation ability of the resultant β-CD derivatives are compared and the β-CD derivatives appended with aromatic imidazole or pyridine provide extremely rich host surroundings for loading Rhodamine B (RB), which is identified by the 1 H NMR titration and UV–Vis spectroscopy. The bi-functional adsorbents correlative to those β-CD derivatives are then synthesized by the polymerization of vinylated β-CD and the corresponding vinyl N-containing monomers , and applied to remove RB and Cd (II) from the aqueous condition. Governed by multiple factors such as porosity, surface charge and binding affinity , the imidazole modified β-CD adsorbent revealed the best adsorption efficiency for organic dyes and metal ions , in both single- and bi-component solutions. Our work provides effective strategy and reliable basis for the design and fabrication of β-CD-based materials with high capacity and multi-functionality.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2020
·Vol. 103
·期 10
·5567-5572
·
IF 3.502
Weide Wang,Dongxu Yao,Hanqin Liang,Yongfeng Xia,Kaihui Zuo,Jinwei Yin,Yu-Ping Zeng
A two-step sintering process was conducted to produce β-Si 3 N 4 ceramics with high thermal conductivity. During the first step, native SiO 2 was eliminated, and Y 2 O 3 was in situ generated by a metal hydride reduction process, resulting in a high Y 2 O 3 /SiO 2 ratio. The substitution YH 2 for Y 2 O 3 endow Si 3 N 4 ceramics with an increase of 29% in thermal conductivity from 95.3 to 123 W m −1 K −1 after sintered at 1900°C for 12 hours despite an inferior sinterability. This was primarily attributed to the purified enlarged grains, devitrified grain boundary phase, and reduced lattice oxygen content in the YH 2 -MgO-doped material.
✓ 已验证
DOI
高影响因子
Materials
·
2020
·Vol. 13
·期 17
·3857
·
IF 3.057
Liao Pan,Meiying Zhang,Lixin Lu,Bingxian Ou,Xi Chen
Antioxidant packaging is an effective method to protect oxygen-sensitive food from oxidation. In order to concurrently obtain a storage stability and excellent oxygen scavenging of antioxidant film for the high moisture food, a moisture activating oxygen scavenging film was prepared by using tea polyphenols as the oxygen scavenger. The moisture activating function was achieved by introducing the β-cyclodextrin embedding technology, and the tea polyphenols–β-cyclodextrin inclusion complex was co-extruded with low-density polyethylene (LDPE) to improve the storage stability. The results indicate that the tea polyphenols is well embedded by β-cyclodextrin according to the Fourier transform infrared spectra (FT-IR), and a relatively homogeneous dispersion of oxygen scavenger is observed while the oxygen scavenger content is less than 5%. The oxygen scavenging increases with the increase of oxygen scavenger from 1% to 5%, and a maximal oxygen absorption of 0.0150 mol/m2is exhibited at oxygen scavenger content value of 5%. Then, the oxygen scavenging significantly decrease under the oxygen scavenger content of 7% and 10%. Moreover, the oxygen scavenging amount sharply increase after steeping in water or storage in extremely high humidity of RH 84% while the oxygen scavenging is restrained under RH 32–75%, indicating that the moisture activating oxygen scavenging is functioning. The oxygen scavenging is obvious restrained under low temperature of 4 °C while the oxygen scavenging is activated at 23 °C and 50 °C with similar oxygen scavenging amount. Besides, both of the tensile and heat-sealing strength deteriorative with the increase of oxygen scavenger content, while they are acceptable at oxygen scavenger content of 5%. Finally, the prepared oxygen scavenging film was used for packaging orange juice and received a good antioxidant effect. Thus, the acquired moisture activating oxygen scavenging film has a good stability under regular storage condition, and shows a potentially application for oxygen-sensitive food with high moisture content.Keywords:moisture activating;oxygen scavenging film;tea polyphenols;β-cyclodextrin;low-density polyethylene (LDPE)
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
·
2020
·Vol. 113
·期 10
·1467-1477
·
IF 2.674
Yin Yi-Rui,Sang Peng,Yang Feng-Li,Li Tao,Yang Run-Feng,Liu Hong-Yan,Luo Zong-Long,Li Wen-Jun,Yang Li-Quan
A β-glucosidase gene ( bsbgl1a ) from Bacillus sp. CGMCC 1.16541 was expressed in Escherichia coli BL21 and subsequently characterized. The amino acid sequence shared 83.64% identity with β-glucosidase (WP_066390903.1) from Fictibacillus phosphorivorans . The recombinant β-glucosidase (BsBgl1A) had a molecular weight of 52.2 kDa and could hydrolyze cellobiose, cellotriose, cellotetrose, p-nitrophenyl-β- d -glucopyranoside ( p NPG), and p-nitrophenyl-β- d -xylopyranoside ( p NPX). Optimal activity for BsBgl1A was recorded at 45 °C with a pH between 5.6 and 7.6, and 100% of its activity was maintained after a 24 h incubation between pH 4 and 9. Kinetic characterization revealed an enzymatic turnover (Kcat) of 616 ± 2 s −1 (with cellobiose) and 3.5 ± 0.1 s −1 (with p-nitrophenyl-β- d -glucopyranoside). Interestingly, the recombinant enzyme showed cupric ion (Cu 2+ ), sodium dodecyl sulfate (SDS) and alcohol tolerance at 10 mM for Cu 2+ and 10% for both SDS and alcohol. Additionally, BsBgl1A had high tolerance for glucose (Ki = 2095 mM), which is an extremely desirable feature for industrial applications. Following the addition of BsBgl1A (0.05 mg/ml) to a commercial cellulase reaction system, glucose yields from sugarcane bagasse increased 100% after 1 day at 45 °C. This work identifies a Cu 2+ , SDS, alcohol, and glucose tolerant GH1 β-glucosidase with potential applications in the hydrolysis of cellulose for the bioenergy industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Jiaying Zhu,Jiaqi Sun,YaJie Tang,Jingli Xie,Dongzhi Wei
A heterodimeric β-galactosidase was discovered in the novel strain Lactobacillus curieae M2011381. The gene encoding the enzyme was expressed in Escherichia coli BL21 (DE3). The specific enzyme activities of the recombinant holoenzyme (LacLM) and large subunit (LacL) measured 11.4 U/mg and 3.8 U/mg, respectively. The k cat /K m values of LacLM and LacL were 740 mM −1 s −1 and 1.40 mM −1 s −1 , respectively. LacLM showed maximum activity at pH 8.0 and 55 °C, and it could maintain its activity at a neutral pH and below 45 °C. LacLM displayed both hydrolysis and transgalactosylation activity on 200 g/L lactose. When LacLM was added to milk, the lactose was hydrolyzed after 6 h without galactooligosaccharide generation. The sequence alignment and homology modeling of the structures of the holoenzyme and subunits revealed that LacL has a catalytic domain with a catalytic dyad, Glu470 and Glu538, and small subunit LacM is a β-sheet domain with a conserved Trp294. The molecular docking of LacLM helped to illustrate the roles of both subunits in the reaction with lactose.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 28
·12201-12207
·
IF 3.288
Yan Qiao,Xionglin Zhou,Hongchao Geng,Leilei Sun,Deshuai Zhen,Qingyun Cai
A β-NaYF4:Yb,Er,Gd nanorods@1T/2H-MoS2 nanocomposite was fabricated and used for photocatalytic sterilization under illumination from single near-infrared (NIR) light. Approximately 99.3% of Escherichia coli were killed effectively by 15 min irradiation of NIR light at a dose of 40 μg mL−1. The effective bactericidal activity is ascribed to the high upconversion luminescence efficiency of β-NaYF4:Yb,Er,Gd and the layered two-dimensional nanosheet structure of 1T/2H-MoS2. Under 980 nm light irradiation, β-NaYF4:Yb,Er,Gd emits visible light, which is absorbed by MoS2, producing strongly oxidative holes and reductive electrons. Photogenerated electron–hole pairs were able to react with H2O, O2 and OH− to produce reactive species in aqueous solution. These reactive species are strong nonselective oxidizing agents for sterilization.
✓ 已验证
产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2020
·
IF 4.268
Chunjun Wang,Jihong Yao,Linjie Ju,Xiaohua Wen,Luan Shu
Background Diabetes is characterized by β-cell loss and dysfunction. A strategy for diabetes treatment is to promote new β-cell formation. Puerarin is an isoflavone from the root of Pueraria lobata (Willd.) Ohwi . Our previous study demonstrated puerarin could ameliorate hyperglycemia in diabetic mice. However, related mechanisms and potential roles of puerarin in β-cell neogenesis have not been elucidated. Purpose The present study aims to investigate whether anti-diabetic effect of puerarin is dependent on promoting β-cell neogenesis via GLP-1R signaling activation. Methods A high-fat diet (HFD) induced diabetic mouse model was applied to investigate effects of puerarin in vivo , exendin-4 (GLP-1R agonist) and metformin were used as positive controls. Moreover, related mechanisms and GLP-1R downstream signal transduction were explored in isolated cultured mouse pancreatic ductal cells. Results Puerarin improved glucose homeostasis in HFD diabetic mice significantly. Markers of new β-cell formation (insulin, PDX1 and Ngn3) were observed in pancreatic ducts of HFD mice treated by puerarin. Of note, efficacy of puerarin in vivo was suppressed by GLP-1R antagonist exendin9-39, but enhanced by exendin-4 respectively. In cultured mouse pancreatic ductal cells, puerarin induced expressions of insulin and PDX1, upregulated GLP-1R expression and activated β-catenin and STAT3 subsequently. Expressions of insulin and PDX1 in ductal cells could be blocked by exendin9-39, or β-catenin inhibitor ICG001, or JAK2 inhibitor AG490 . Conclusion These data clarified puerarin ameliorated hyperglycemia of HFD mice via a novel mechanism involved promoting β-cell neogenesis. Our finding highlights the potential value of puerarin developing as an anti-diabetic agent.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Yueran Zhu,Yunguang Lu,Longyan Shi,Yaling Yang
A green, reliable and simple assay for Fluoroquinolone derivatives (FQs) is developed based on Mono-(6-Amino-6-Deoxy)-β-Cyclodextrin (β-CD) were modified on the surface of N,Zn co-doped carbon dots (N,Zn-CDs). FQs not only enhance β-CD-N,Zn-CDs fluorescence intensity, but also red-shift. Since FQs has the same effect on the fluorescence of β-CD-N,Zn-CDs, so ofloxacin (OFL) as a representative for analysis. Under optimized detection conditions, the linear range is 0.075–3.75 μM and the LOD is 0.05 μM (S/N = =3). Finally, the proposed method was successfully applied in the determination of OFL in milk and domestic water with satisfactory recoveries.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）