SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Zi-Ming Zhou,Yong Yu,Ming-Zhen Zhang,Jun Chen,Qiong-Qiong Ren,Ji-Tao Song,Bo Liu,Zhi-Ya Ma,Yuan-Di Zhao
It is important to design new ways with high specificity and sensitivity to detect lead (II). Here, ‘8–17’ DNAzyme was used as a Pb 2+ -dependent probe with high sensibility and selectivity based on chemiluminescence (CL). When Pb 2+ was present, the substrate strand was cleaved and the active hemin/G-quadruplex DNAzyme structure was formed in the presence of another sequence which contained two parts, one could be complementary with part of the substrate strand and the other was a G rich subunit. The active hemin/G-quadruplex DNAzyme structure could catalyze luminol oxidation and quantitative analysis of Pb 2+ was carried out according to the CL of luminol. Results showed that CL intensity of luminol at 452 nm had a linear relation with the concentration of Pb 2+ in the range from 5 nM to 1 μM, and the limit of detection was 0.79 nM ( S / N = 3). This method was also used to detect the contents of Pb 2+ in lake water and tobacco and the results (62.4 ± 4.0 nM and 15.2 ± 2.2 nM, respectively) were extremely closed to those of inductively coupled plasma-mass spectrometry (ICP-MS) (57.0 ± 1.4 nM and 14.5 ± 0.91 nM, respectively).
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TALANTA
·
2013
·
IF 3.498
Yuezhen He,Xiaoxun Wang,Yuzhong Zhang,Feng Gao,Yongxin Li,Hongqi Chen,Lun Wang
In this work, we reported a sensitive chemiluminescent immunosensor for the detection of human leptin by using hemin/G-quadruplex DNAzymes to amplify detection signal. In this sensing system, the primary antibody (anti-human leptin) was firstly bound to the 96-well plates, and human leptin and biotinylated secondary antibody were successively combined to form sandwich-type immune complex through specific interactions. Then streptavidin labeled with hemin/G-quadruplex DNAzymes was assembled to the sandwich-type immunocomplex by streptavidin–biotin interaction. The DNAzymes exhibited an excellent catalytic activity to the chemiluminescent reaction of luminol with hydrogen peroxide in strong alkaline solution, leading to significant enhancement in response signal. Under the optimum conditions, the proposed immunosensor showed high sensitivity and selectivity with a low detection limit of 1.9 pg mL −1 and a wide linear response range of human leptin from 10 to 1000 pg mL −1 . The immunosensor was used to detect human leptin in serum, and the results were in good agreement with the data obtained by conventional ELISA method.
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2013
·
IF 5.695
Chun Yang,Lei Liu,Tao Zeng,Daowu Yang,Zhiyi Yao,Yuliang Zhao,Hai-Chen Wu
Both Pb2+ and Ba2+ can bind with high affinity to some specific DNA sequences, inducing the formation of unimolecular G-quadruplex structures. Translocation of a DNA probe containing such sequences through an α-hemolysin nanopore in the presence of Pb2+ or Ba2+ would result in much prolonged DNA translocation events. Quantification of these events can reveal the concentrations of Pb2+ or Ba2+ at as low as 0.8 nM. Besides, Pb2+ and Ba2+ in the solution can be simultaneously detected and individually identified. Furthermore, the probe is highly selective for Pb2+ and Ba2+ detection without interference from other metal ions. This sensing strategy can be extended to many other analytes which can bind to DNA molecules with high affinity.
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PubMed
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2015
·Vol. 30
·期 3
·160-166
·
IF 2.248
Yi Zhang,Rongying Wen,Di Guo,Hongxu Guo,Jianhua Chen,Zishan Zheng
ZnS@g-C 3 N 4 composite photocatalyst containing g-C 3 N 4 nanoplates and ZnS nanoparticles was facilely fabricated by one-step calcination from the complex sulfatotris(thiourea)zinc(II) for the first time. Thiourea serves not only as the sulfur source of ZnS, but also as the precursor of g-C 3 N 4 . The as-prepared composite photocatalyst was then characterized using Fourier transform infrared, diffuse reflectance and X-ray photoelectron spectroscopies, X-ray diffraction, scanning electron microscopy and Brunauer–Emmett–Teller surface area measurements. It displays a higher visible-light photocatalytic activity, including an ability to photodecompose water to produce hydrogen and organic dye photodegradation, than ZnS alone. The photocatalytic mechanism is also discussed in detail. Photoluminescence spectra and electrochemical impedance spectroscopy Nyquist plots indicate that the formation of the ZnS@g-C 3 N 4 composite contributes to the separation of photoinduced charge carriers which enhances its photocatalytic activity. This study presents a new method for the fabrication of novel sulfide/g-C 3 N 4 composites with enhanced photocatalytic performance. Copyright © 2015 John Wiley & Sons, Ltd.
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BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Fenglei Gao,Taotao Fan,Jing Wu,Sha Liu,Yan Du,Yao Yao,Fuyi Zhou,Yu Zhang,Xianjiu Liao,Deqin Geng
We describe a novel label-free and signal-on electrochemical DNA sensing platform via proximity hybridization triggered hemin/G-quadruplex formation based on the direct electron transfer of hemin . The thiolated modified G-DNA1 was first immobilized onto the Au electrode surface . In the presence of target DNA, Y-junction-structure ternary complex can be formed to trigger the proximity assembly of G-DNA1, hemin, and G-DNA2, which leads to the formation of hemin/G-quadruplex for generation an amplified electrochemical signal by differential pulse voltammetry . The observed signal gain was sufficient to achieve a demonstrated detection limit of 54 fM, with a wide linear dynamic range from 10 −13 to 10 −9 M and discriminated mismatched DNA from perfect matched target DNA with a high selectivity. Benefiting from the one step proximity dependent hemin/G-quadruplex formation, the assay method is extremely simple and can be carried out within 40 min. The advantages of free of any label conjugation step, and versatility make it a promising candidate for point-of-care testing and commercial application.
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PubMed
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2017
·
IF 4.339
Xuli Miao,Xiaoping Shen,Jiajia Wu,Zhenyuan Ji,Jiheng Wang,Lirong Kong,Miaomiao Liu,Chunsen Song
In this work, an excellent ternary visible-light all solid Z-scheme photocatalytic heterojunction of g-C 3 N 4 /GO/AgBr has been prepared by the attachment of graphene oxide (GO) to the surface of g-C 3 N 4 , followed by in-situ growth of AgBr nanoparticles on GO sheets. The g-C 3 N 4 /GO/AgBr heterojunction exhibits excellent photocatalytic efficiency for RhB degradation, which is about 17.5, 7.9, 3.5, 4.0 and 2.2 times as high as that of P25, g-C 3 N 4 , AgBr, g-C 3 N 4 /GO and g-C 3 N 4 /AgBr heterojunction, indicating the introduction of GO into the system largely improved the capability of the photocatalysis since GO acted as the charge transmission bridge between g-C 3 N 4 and AgBr. Moreover, the photocatalytic performance of g-C 3 N 4 /GO/AgBr just showed a slight decrease after 4 degradation cycles, demonstrating a high stability of the g-C 3 N 4 /GO/AgBr photocatalyst. The photocatalytic mechanism investigation indicated that O 2 − and h + are the major active species in the photocatalytic process. It is expected that the GO-inserted Z-scheme photocatalytic heterojunction could be applied to practical pollutant degradation.
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高影响因子
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
·
2017
·Vol. 2017
·期 40
·4797-4802
·
IF 2.444
Yulan Peng,Lingzhi Wang,Yongdi Liu,Haijun Chen,Juying Lei,Jinlong Zhang
Graphical A CoP-decorated g-C 3 N 4 composite catalyst was developed for highly efficient and stable photocatalytic production of H 2 O 2 under visible-light irradiation. The CoP nanoparticles act as a noble metal free cocatalyst for g-C 3 N 4 to enhance the visible-light absorption and promote the separation of photogenerated charge carriers. A composite catalyst combining CoP with g-C 3 N 4 was explored for the photocatalytic production of H 2 O 2 under visible-light irradiation. The composite catalyst was fabricated by growth of CoP nanoparticles on the surface of g-C 3 N 4 . The CoP nanoparticles were well dispersed on the surface of g-C 3 N 4 and could interact with g-C 3 N 4 to improve charge separation and electron transfer. The composite catalyst showed superior catalytic activity compared with pure CoP or g-C 3 N 4 under visible-light irradiation. The optimal catalyst with 1.76 wt.-% CoP loading exhibited the best photocatalytic efficiency with an H 2 O 2 production of 140 µ m in 2 h, which is about 4.6 and 23.3 times those of pure g-C 3 N 4 and pure CoP, respectively. A possible mechanism for the visible-light-driven photocatalytic production of H 2 O 2 is proposed. The composite catalyst exhibited stable performance without obvious loss of catalytic activity after seven successive runs, and thus has good application prospects in sustainable H 2 O 2 production.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2016
·Vol. 51
·期 14
·6998-7007
·
IF 2.302
Deng Fang,Lu Xiaoying,Zhao Lina,Luo Yiting,Pei Xule,Luo Xubiao,Luo Shenglian
Hierarchical network-like graphitic carbon nitride/SnIn 4 S 8 (g-C 3 N 4 /SnIn 4 S 8 ) composites were prepared through a facile low-temperature co-precipitation method. The g-C 3 N 4 /SnIn 4 S 8 composite showed enhanced visible-light absorption. The band gap energies of g-C 3 N 4 , pure SnIn 4 S 8 , and 15 % g-C 3 N 4 /SnIn 4 S 8 are 2.58, 1.8, and 1.68 eV, respectively. The photocurrent and photocatalytic activity of the g-C 3 N 4 /SnIn 4 S 8 composites firstly increased and then decreased with increasing g-C 3 N 4 content, and it was found that the optimal 15 % g-C 3 N 4 /SnIn 4 S 8 exhibited the highest photocurrent intensity and best photocatalytic performance with complete degradation of MO within 80 min under visible-light irradiation, which is much higher than that of pure SnIn 4 S 8 . The effect of main reactive species on the MO degradation follows the order of h + > • O 2
− > • OH, and the possible degradation mechanism was proposed. Moreover, 15 % g-C 3 N 4 /SnIn 4 S 8 exhibits excellent reusability and stability without an obvious decrease of photocatalytic activity after four consecutive photocatalytic degradation–regeneration cycles.
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高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Yu Fan,Xingrong Tan,Xin Ou,Qiyi Lu,Shihong Chen,Shaping Wei
In this work, a novel “on-off” electrochemiluminescence (ECL) biosensor was successfully constructed for ultrasensitive detection of concanavalin A (ConA). The “signal-on” state with strong initial ECL signal was obtained by Ag-doped graphitic carbon nitride nanosheet (Ag-g-C 3 N 4 ), which was modified onto the glassy carbon electrode (GCE) for immobilizing phenoxy dextran (DexP) through π-π interaction. Then, the target ConA was anchored onto the DexP/Ag-g-C 3 N 4 modified film via the specific carbohydrate-ConA interaction. Successively, polyaniline-3,4,9,10-perylenetetracarboxylic acid-DexP conjugate (denoted as PANI-PTCA-DexP), as the ECL signal quenching probe, was incubated onto the electrode through the carbohydrate-ConA interaction to achieve the “signal-off” state. Here, PTCA was used as a matrix for the high loading of PANI and DexP, PANI served as the quencher towards Ag-g-C 3 N 4 system, and DexP as the recognition element for bounding ConA. With the formation of the sandwiched structure of DexP, ConA and PANI-PTCA-DexP, a desirable quenching ECL signal was measured with S 2 O 8 2− as the co-reactant of Ag-g-C 3 N 4 . The quenching effect of PANI towards Ag-g-C 3 N 4 is positively correlated with the concentration of ConA. With such an “on-off” system, the detection of ConA was achieved with a wide linear range from 0.001 ng/mL to 50 ng/mL as well as a low detection limit down to 0.0003 ng/mL.
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高影响因子
RSC Advances
·
2017
·Vol. 7
·期 39
·24097-24104
·
IF 3.108
Jie Meng,Jingyuan Pei,Zefang He,Shiyan Wu,Qingyun Lin,Xiao Wei,Jixue Li,Ze Zhang
Graphitic carbon nitride (g-C3N4) nanosheets loaded with WO3 nanoparticles were prepared via heat treatment of g-C3N4 together with WOx-EDA nanobelts. The thermal treatment temperature and WOx-EDA precursor play the key role in enhancing the interaction between WO3 nanoparticles and g-C3N4 nanosheets, because the temperature of thermal decomposition of WOx-EDA (∼400 °C) is close to the thermal exfoliation temperature of g-C3N4. The structure evolution and promotion effect of the nanocomposites in photocatalytic performance were well studied. It is found that WO3 nanoparticles uniformly dispersed on the surface of the g-C3N4 nanosheets, and the close integration of WO3 and g-C3N4 lead to the high photocatalytic activity in the degradation of RhB under visible light irradiation. Meanwhile, a larger specific area and increase of visible light absorption are also of benefit to speed up the degradation of organic dye. Also, the mechanism of the photocatalytic reaction and its reutilization properties were investigated.
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高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2016
·Vol. 99
·期 6
·2131-2137
·
IF 2.787
YeHong Cheng,Yushi Qi,Ping Hu,Shanbao Zhou,Guiqing Chen,Jiadong An,Kunfeng Jin,Wenbo Han
To avoid introduction of milling media during ball-milling process and ensure uniform distribution of SiC and graphite in ZrB 2 matrix, ultrafine ZrB 2 –SiC–C composite powders were in-situ synthesized using inorganic–organic hybrid precursors of Zr(OPr) 4 , Si(OC 2 H 5 ) 4 , H 3 BO 3 , and excessive C 6 H 14 O 6 as source of zirconium, silicon, boron, and carbon, respectively. To inhabit grain growth, the ZrB 2 –SiC–C composite powders were densified by spark plasma sintering (SPS) at 1950°C for 10 min with the heating rate of 100°C/min. The precursor powders were investigated by thermogravimetric analysis–differential scanning calorimetry and Fourier transform infrared spectroscopy. The ceramic powders were analyzed by X-ray diffraction, X-ray photoelectron spectroscopy, and scanning electron microscopy. The lamellar substance was found and determined as graphite nanosheet by scanning electron microscopy, Raman spectrum, and X-ray diffraction. The SiC grains and graphite nanosheets distributed in ZrB 2 matrix uniformly and the grain sizes of ZrB 2 and SiC were about 5 μm and 2 μm, respectively. The carbon converted into graphite nanosheets under high temperature during the process of SPS. The presence of graphite nanosheets alters the load-displacement curves in the fracture process of ZrB 2 –SiC–G composite. A novel way was explored to prepare ZrB 2 –SiC–G composite by SPS of in-situ synthesized ZrB 2 –SiC–C composite powders.
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高影响因子
APPLIED SURFACE SCIENCE
·
2016
·
IF 3.15
Zhi Zhu,Xu Tang,Changchang Ma,Minshan Song,Nailing Gao,Youshan Wang,Pengwei Huo,Ziyang Lu,Yongsheng Yan
The ternary conductive Ppy@Ag/g-C 3 N 4 composite photocatalysts was successfully synthesized by polymerization process and surface polymerization technique. And the as-prepared Ppy@Ag/g-C 3 N 4 sample exhibited the higher photocatalytic activity for various pollutant (MO, DM, TC, CIP, GFLX and EH) remove than that of pure g-C 3 N 4 and Ag/g-C 3 N 4 under visible light irradiation. It mainly originated from the Ag nanoparticles anchored between g-C 3 N 4 and Ppy acted as electron transfer mediator that facilitated the charge carrier separation and then expending the lifetime of the carriers. Meanwhile, the obtained Ppy@Ag/g-C 3 N 4 sample also showed a relatively good recycling stability which was the crucial factor for photocatalyst practical application. This work provided a new facile strategy for improving photo-degradation activity of g-C 3 N 4 photocatalyst.
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