ANALYST
·
2023
·Vol. 148
·期 9
·2081-2091
·
IF 4.2
Hanlin Guo,Yu Li,Fuwei Pi
Given the prevalence of food safety, online monitoring of food quality is essential. Surface-enhanced Raman scattering (SERS) has excellent sensitivity and molecular fingerprinting capabilities in analytical fields, but its accuracy in food safety monitoring is severely constrained, particularly for gaseous molecules. To further develop the SERS technique in food sensing, in this work, a slippery liquid-infused porous surface (SLIPS) platform was developed for the real-time monitoring of the change in gaseous molecules in shrimp spoilage processes. In order to monitor the change in pH and gaseous biogenic amine molecules (BAs), 4-mercaptopyridine (4-Mpy) and 4-mercaptobenzaldehyde (4-MBA)-functionalized ZIF-8-encapsulated gold nanostars (AuNS@ZIF-8) were utilized as response probes, respectively. Due to the superior gaseous molecule trapping ability of ZIF-8 and the excellent enrichment effect of SLIPS substrates, the use of 4-Mpy and 4-MBA-functionalized AuNS@ZIF-8-SLIPS substrates exhibited excellent online SERS sensing performance for pH and gaseous putrescine molecules. The detection ranges for pH and gaseous BAs were 4.0–9.0 and 10−7–10−3 (v/v) with RSDs of 4.1% and 4.2%, respectively. Furthermore, the SERS monitoring platform was used to monitor shrimp spoilage at 25 °C and 4 °C in real time. Hence, the AuNS@ZIF-8-SLIPS membrane strategy can serve as a promising alternative to ensure accurate, real-time, and non-destructive monitoring of gaseous molecules for food freshness.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Dechan Lu,Rongyuan Cai,Yuqin Liao,Ruiyun You,Yudong Lu
Abnormal uric acid (UA) content in body fluids can fully reflect the status of metabolism and immunity in the body. We have developed a simple, efficient and label-free surface enhanced Raman scattering (SERS) method for UA detection. Briefly, p-aminothiophenol (p-ATP) was used as the internal standard molecule and linking molecule to prepare a glass/p-ATP/Ag NPs SERS substrate. The Raman characteristic peak of p-ATP at 1076 cm −1 can be used as an internal standard molecule to correct the signal fluctuation of UA detection. The results show that the SERS method owns a linear response with a ranging from 5 × 10 -6 to 10 -3 M of UA characteristic peak of both 693 cm −1 and 493 cm −1 with a determination coefficient (R 2 ) of 0.9878 and 0.9649, respectively. Additionally, the SERS sensor has been further used for the analysis of UA in sweat and good recoveries were obtained for the sensing of sweat. We believe that the developed SERS substrate has potential for applications in healthcare monitoring.
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Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Xiong Junfeng,Zhang Junkai,Zhong Yukun,Song Xiaoxia,Wang Haoying,Cheang U Kei
Ultraviolet lithography is a very promising technology used for the batch fabrication of biomedical microswimmers. However, creating microswimmers that can swim at low Reynolds number using biocompatible materials while retaining strong magnetic properties and excellent biomedical functionality is a great challenge. Most of the previously reported biomedical microswimmers possess either strong magnetic properties by using non-biocompatible nickel coating or good biocompatibility by using iron oxide particle-embedded hydrogel with weak magnetism, but not both. Alternatively, iron oxide nanoparticles can be coated on the surface of microswimmers to improve magnetic properties; however, this method limited the usability of the microswimmers’ surfaces. To address these shortcomings, this work utilized an in situ synthesis technique to generate high magnetic content inside hydrogel-based achiral planar microswimmers while leaving their surfaces free to be functionalized for SERS detection. The hydrogel matrices of the magnetically actuated hydrogel-based microswimmers were first prepared by ultraviolet lithography. Then, the high concentration of iron oxide was achieved through multiple continuous in situ coprecipitation cycles. Finally, the SERS detection capability of magnetically actuated hydrogel-based microswimmers was enabled by uniformly growing silver nanoparticles on the surface of the microswimmers. In the motion control tests, the microswimmers showed a high swimming efficiency, high step-out frequency, and consistent synchronized motion. Furthermore, the magnetically actuated hydrogel-based microswimmers were able to improve the detection efficiency of analytes under magnetic guidance.
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Advanced Sustainable Systems
·
2022
·Vol. 7
·期 2
·2200387
·
IF 6.737
Meng He,Zhihao Li,Yujie Guo,Maofeng Zhang,Zhengdong Zhu,Xiaoyi Shi,Konghao Han,Youju Huang,Honglin Liu
The practical application of surface-enhanced Raman spectroscopy (SERS) technology for quantitative analysis still confronts the hinder of controlling the uniformity of signal and anchoring target molecules in a designed space. Herein, a facile method of devising the shrinkage of three-dimension (3D) hot spots droplets on a hydrophobic array substrate is demonstrated for the quantitative and multiplex detection of pesticide residues by using a portable Raman spectrometer. The shrunk droplet which is consisted of the analyte solution and AgNPs sol can induce simultaneously increasing density of plasmonic hot spots and immobilizing target molecules in hot spots. Besides, the closely packed Ag nanoparticles (AgNPs) are anchored in the gaps of the MoS 2 nanosheet array, generating uniform hotspots distribution with relative standard deviation (RSD) of about 8 %. In addition, the synergetic effects between MoS 2 and AgNPs further enhance the SERS sensitivity, and thereby target molecules of 10 −9 m are readily detected. Besides, the proposed strategy exhibits excellent SERS sensitivity for the analysis of pesticides down to 10 −8 m . Importantly, quantitative analysis and multi-components detection of pesticide residues on honeysuckle surface are achieved. Therefore, it is expected to be used for quantitative analysis of complex pesticide systems on real samples.
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Nanomaterials
·
2023
·Vol. 13
·期 2
·334
·
IF 5.3
Jie Li,Qiutian She,Wenxi Wang,Ru Liu,Ruiyun You,Yaling Wu,Jingzheng Weng,Yunzhen Liu,Yudong Lu
Label-free surface-enhanced Raman scattering (SERS) analysis shows tremendous potential for the early diagnosis and screening of colon cancer, owing to the advantage of being noninvasive and sensitive. As a clinical diagnostic tool, however, the reproducibility of analytical methods is a priority. Herein, we successfully fabricated Ag NPs/cellulose nanocrystals/graphene oxide (Ag NPs/CNC/GO) nanocomposite film as a uniform SERS active substrate for label-free SERS analysis of clinical serum. The Ag NPs/CNC/GO suspensions by self-assembling GO into CNC solution through in-situ reduction method. Furthermore, we spin-coated the prepared suspensions on the bacterial cellulose membrane (BCM) to form Ag NPs/CNC/GO nanocomposite film. The nanofilm showed excellent sensitivity (LOD = 30 nM) and uniformity (RSD = 14.2%) for Nile Blue A detection. With a proof-of-concept demonstration for the label-free analysis of serum, the nanofilm combined with the principal component analysis-linear discriminant analysis (PCA-LDA) model can be effectively employed for colon cancer screening. The results showed that our model had an overall prediction accuracy of 84.1% for colon cancer (n= 28) and the normal (n= 28), and the specificity and sensitivity were 89.3% and 71.4%, respectively. This study indicated that label-free serum SERS analysis based on Ag NPs/CNC/GO nanocomposite film combined with machine learning holds promise for the early diagnosis of colon cancer.Keywords:graphene oxide;label-free;PCA-LDA;colon cancer;SERS
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xuejiao Wang,Xupeng Zhu,Yufeng Tao,Erjin Zhang,Xudong Ren
Pesticide residues threaten the ecological environment and human health. Therefore, developing high performance SERS substrate to achieve highly sensitive detection of pesticide residues is meaningful. In this study, based on the strategy of combining “hot spots” engineering and material hybridization, we construct a novel hybrid SERS substrate by depositing Ag nanoflowers (NFs) on ZnO nanorods (NRs). Benefiting from the synergistic effect of electromagnetic enhancement and charge transfer effect, the Ag NFs@ZnO NRs substrate exhibits a low detection limit (10 −13 M) for crystal violet molecules. This SERS substrate has good uniformity with a relative standard deviation of 7.463 %. Besides, owning to the photocatalytic property of ZnO NRs, the hybrid substrate can degrade probe molecules after SERS detection and realize recyclability. As a demonstration, we employed our SERS substrate for the trace detection of pesticide residues on apple surface and in river water. This study provides a new idea for improving the SERS performance of hybrid substrates.
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ACS Nano
·
2023
·
IF 17.1
Xiaoming Su,Xinyu Liu,Yangcenzi Xie,Mingyang Chen,Chao Zheng,Hong Zhong,Ming Li
Protein profiles of exosomes (EXOs) in clinical samples of cancer patients have become a promising diagnostic and therapeutic biomarker. However, simultaneous quantitative analysis of multiple exosomal proteins of interest remains challenging. To address the unmet need, we develop a paper-based surface-enhanced Raman spectroscopy (SERS)-vertical flow biosensor, named iREX (integrated Raman spectroscopic EXO) biosensor, for multiplexed quantitative profiling of exosomal proteins in clinical serum samples of patients. Utilizing this iREX biosensor, we are able to quantitatively profile MUC1, HER2 and CEA in EXO samples derived from various breast cancer cell subtypes. The results show discriminative expression profiles of the three exosomal proteins in these cell subtypes, which allows for accurate diagnosis and molecular subtyping of breast cancer. We further validate the clinical utility of the iREX biosensor for simultaneous quantitative analysis of MUC1, HER2 and CEA in patient’s blood serums, thereby aiding in noninvasive breast cancer subtyping and longitudinal treatment monitoring. Our iREX biosensor integrating the SERS detection in a vertical flow diagnostic device offers great advantages of high sensitivity, molecular specificity, powerful multiplexing capability, and high diagnostic accuracy. We believe that the iREX biosensor could be a promising clinical tool for comprehensive analysis of exosomal proteins in clinical samples for personalized diagnosis and precise management of breast cancer.
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JOURNAL OF MATERIALS RESEARCH
·
2022
·Vol. 38
·期 2
·519-531
·
IF 2.909
Zhu Sijia,Wang Qing,Yang Jinhui,Li Hongmei,Gao Ningning,Zhong Furu,Fu Jihong,Tang Jun,Wang Tao
Biomass-derived porous carbon (PC) loaded with precious metals have a synergistic enhancement effect on surface-enhanced Raman scattering (SERS). In this paper, PC containing various functional groups and large specific surface area, good stability and certain biocompatibility were prepared using tomato skins and introduced into the preparation of Ag nanoflowers (NFs) SERS substrates. Rhodamine 6G (R6G) was used as the Raman probe molecule to evaluate the sensing performance of Ag NFs@PC. The results showed that the stable dispersion and protective effect of PC on Ag NPs significantly improved the sensitivity and long-term stability of traditional Ag NFs. In practical application, Ag NFs@PC was further used to successfully achieve the quantitative analysis of trace methylene blue (10 ppt) and malachite green (10 ppt) in a lake water system. Therefore, SERS sensor Ag NFs@PC is expected to become a promising candidate sensor for detecting in the field of environment and food monitoring.
Graphical abstract
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Analytical Methods
·
2023
·Vol. 15
·期 37
·4851-4861
·
IF 3.1
Rui Yang,Baowen Zhang,Ya Wang,Yi Zheng,Qian Zhang,Xiupei Yang
Substrate materials with high sensitivity and storage stability are crucial for the practical analytical application of surface-enhanced Raman scattering (SERS) techniques. In this work, a SERS-active substrate (Si/Au@Ag/ZIF-67) was fabricated with a metal–organic framework (ZIF-67) on a plasmonic surface (Si/Au@Ag) via self-assembly. The as-prepared material combined the properties of the abundant hotspots of the Au@Ag nanoparticles and the excellent adsorption performance of ZIF-67 for organic molecules. The synergy leads to high sensitivity of the composite substrate with a low detection limit for 4-aminothiophenol (a typical Raman reporter molecule) down to 2.0 × 10−9 M and the analytical enhancement factor (AEF) of the SERS substrate is 3.4 × 106. Moreover, the substrates exhibited good repeatability, high reproducibility, and reliable stability due to the MOF coating. The SERS signal was stable after 60 days of storage at room temperature. Ultimately, the optimal Si/Au@Ag/ZIF-67 was applied as a SERS sensor to analyze thiram, and the results showed a linear concentration range from 10−7 to 10−5 M with good linearity (R2 = 0.9934). The recoveries of thiram in spiked apple juice were in the range of 95.7–102.3%, with relative standard deviations less than 4.3%. These results predict that the proposed SERS substrates may hold great potential for the detection of environmental and food pollution in practical applications.
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Nanomaterials
·
2023
·Vol. 13
·期 13
·2016
·
IF 5.3
Yanyan Lu,Xuzhou Yuan,Cuiping Jia,Biao Lei,Hongwen Zhang,Zhipeng Zhao,Shuyi Zhu,Qian Zhao,Weiping Cai
Volatile organic compounds (VOCs), particularly monoaromatic hydrocarbon compounds (MACHs), pose a potential risk to the atmospheric environment and human health. Therefore, the progressive development of efficient detection methodologies is a pertinent need, which is still a challenge at present. In this study, we present a rapid and sensitive method to detect trace amounts of MACHs using a bifunctional SERS composite substrate. We prepared an Au/SiO2enhanced layer and a porous Cu(OH)2adsorption layer via microfluidic-assisted gas-liquid interface self-assembly. The composite substrate effectively monitored changes in benzaldehyde using time-varying SERS spectra, and track-specifically identified various VOCs such as benzene, xylene, styrene, and nitrobenzene. In general, the substrate exhibited a rapid response time of 20 s to gaseous benzaldehyde, with a minimum detection concentration of less than 500 ppt. Further experimental assessments revealed an optimum Cu(OH)2thickness of the surrounding adsorption layer of 150 nm, which can achieve an efficient SERS response to MACHs. Furthermore, the recoverable and reusable property of the composite substrate highlights its practicality. This study presents a straightforward and efficient approach for detecting trace gaseous VOCs using SERS, with significant implications in the designing of SERS substrates for detecting other VOCs.Keywords:VOCs;bifunctional copper hydroxide/gold ordered nanoarray composites;self-assembly;fast and sensitive SERS detection;recyclable SERS substrate
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Dechan Lu,Qun Zhang,Zufang Huang,Yudong Lu,Shangyuan Feng,Ruiyun You,Minling Li,Shaoqin Zhang
Tyrosinase (TYR) is a polyphenol oxidase that regulates melanin biosynthesis . Abnormal levels of TYR have been confirmed closely associated with melanoma cancer and other diseases, making the establishment of highly sensitive and accurate quantitative detection of TYR is thus essential for fundamental research and clinical applications. Herein, we proposed a new strategy that combines surface-enhanced Raman scattering (SERS) with Dopamine (DA) and Prussian blue (PB) functionalized gold-gold hybrid nanoparticles for TYR detection. DA is oxidized to dopaquinone with the presence of TYR, leading to the consumption of DA in the reaction solution and the corresponding decrease in DA characteristic peak intensity at 1480 cm −1 . Our SERS quantitative assay of TYR with “on-off” sensing strategy yields an excellent limit of detection (LOD) of 3 × 10 −3 U mL −1 in a linear range of 10 −3 to 100 U mL −1 . In particular, the intensity ratio of 1480 cm −1 to 2121 cm −1 allows us to achieve remarkably reliable quantitative detection of TYR, with the 2121 cm −1 peak intensity of the standard internal (IS) molecule PB being used to correct SERS signal fluctuations. Furthermore, our proposed assay has been successfully demonstrated to quantify TYR spiked in human serum samples, with excellent percentage recovery of 90.0–110.6 %. The potential of our ratiometric SERS strategy for the performance evaluation of TYR inhibitors has also been verified. Our work is therefore expected to provide a new route for accurate and reliable monitoring of TYR activity in the complex biological environment.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Suyang Li,Pei Liang,Qiang Chen,Biao Sun,Ziyang Shang,Jie Huang,Mingqiang Zou,Xiaohua Qi,Jiechen Wu
A new Mo 1- x W x S 2 two-dimensional nanosheets were prepared by the one-pot method. After certain Mo atoms in MoS 2 were replaced by W ones in a hydrothermal reduction procedure, Mo 1- x W x S 2 was formed on the Mo foil. Well enhanced Mo 1- x W x S 2 nanosheets were prepared when the sodium tungstate concentration got under control. Various characterizations were carried out, which indicate that Mo 1- x W x S 2 nanosheets with good crystallinity. Compared with MoS 2 , the Raman intensity of Rhodamine 6G (10 −6 M) was amplified by 1.7 times with Mo 1- x W x S 2 nanosheets as the substrate. The characteristic Raman peaks could still be clearly distinguished until the concentration of Rhodamine 6G (R6G), Methylene blue (MB) and Crystal violet (CV) down to 10 −8 , 10 −8 and 10 −7 M, respectively. With abundant edge active sites that facilitate charge transfer, Mo 1- x W x S 2 nanosheets could better enhance SERS signals of target detection molecules and get a good linear relationship exists within the concentration and Raman peak strength. In addition, R6G SERS detection also shows excellent reproducibility and long-term stability of this TMDs SERS substrate.
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