SPECTROSCOPY LETTERS
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2025
·
IF 1.6
Ju Wei,Bingyu Luo,Jiao Fu,Shunli Zhou,E. Li,Xiaodong Su,Xiaotong Gao,Zhenfu Jia,Xiaojiang Li
To overcome the interference caused by the inner filter effect, this study optimally selected red fluorescence carbon dots as the signal source and constructed a novel dual-mode Ag@Red-CDs nanoprobe for the highly selective detection of Fe³+ using fluorescence and colorimetry. This probe is based on a unique reaction between Fe3+ and silver nanoparticles (AgNPs) that weakens the static quenching effect of AgNPs on R-CDs; at the same time, the depletion of AgNPs induces a change in the absorbance of the system. Under optimal conditions, the probe exhibited a linear fluorescence response in the Fe³+ concentration range of 0.10–25.00 μg/mL and a linear colorimetric response of 0.18–8.00 μg/mL. The detection limits for fluorescence and colorimetry were 0.31 μg/mL and 0.56 μg/mL, respectively. In spiked real samples, the fluorescence method achieved Fe³+ recovery rates of 99.10–102.34%, while the colorimetric method showed recovery rates of 99.83–101.42%. Overall, this research significantly broadened the potential applications of R-CDs-based materials for the quantitative detection of heavy metal ions in water bodies.
研究领域:
分析化学
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环境科学
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材料科学
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高影响因子
CANCER LETTERS
·
2025
·
IF 10.1
Hongchengcheng Chen,Guihui Yu,Haodong Lin,Xiao Albert Zhou,Jiansong Liu,Haoyun Liu,Yefei Luo,Kaiqi Cheng,Zuchao Mao,Yujie Ma,Jun Wang,Kezhong Chen,Jiadong Wang,Yun Li
R-loop accumulation has emerged as a critical factor that induces DNA damage and compromises genomic integrity. However, the regulatory mechanisms governing the R-loop-induced DNA damage remain unclear. Here, FTSJ3 was determined to be a pivotal regulator of R-loop homeostasis and genomic stability. We demonstrated that FTSJ3 was specifically recruited to R-loop structures, where it prevented DNA damage by suppressing excessive R-loop formation. FTSJ3 expression was significantly upregulated in multiple cancer types, and its elevated expression levels correlated with unfavorable survival in patients with lung adenocarcinoma (LUAD). FTSJ3 depletion increased R-loop-dependent DNA damage. Inhibiting FTSJ3 expression sensitized lung cancer cells to cisplatin both in vitro and in vivo . FTSJ3 could be a genome guardian that limits R-loop-associated damage, suggesting its potential role as a cancer intervention therapeutic target and a predictive biomarker for chemotherapy responsiveness.
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高影响因子
Bioresources and Bioprocessing
·
2019
·Vol. 6
·期 1
·1-12
·
IF 0
Zhang Can,Song Wei,Liu Jia,Chen Xiulai,Liu Liming
( R )- or ( S )-2-Hydroxy-4-(methylthio)butanoic acid (HMTBA) is used as a poultry nutritional supplement and to treat renal failure disease. Herein, we report an artificially designed biocatalytic cascade process, which uses l -methionine to synthesize ( R )- or ( S )-HMTBA. This biocatalysis cascade comprises a basic module and two different extender modules and operates in a modular assembly manner. The basic module responsible for the transformation of l -methionine to α-keto-γ-methylthiobutyric acid (KMTB) is comprised of the l -amino acid deaminase. Two different extender modules responsible for the transformation of KMTB to ( R )- or ( S )-HMTBA are comprised of the R/S-specific lactate dehydrogenase in combination with the formate dehydrogenase, respectively. Engineered Escherichia coli catalysts, one containing the basic module, the other containing the one of two different extender modules, produced 97.6 g L −1 ( R )-HMTBA and 96.4 g L −1 ( S )-HMTBA with a yield of 96.9% and 95.8% at the large scale (1 L) using a two-stage strategy in one pot, respectively. Therefore, this biocatalytic process lays the foundation for the industrial-scale conversion of low-cost l -amino acids to corresponding high-value enantiopure chiral 2-hydroxy acids.
研究领域:
生物技术
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酶工程
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代谢工程
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合成生物学
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Journal of Ginseng Research
·
2022
·
IF 5.735
Hong-Yan Guo,Yue Xing,Yu-Qiao Sun,Can Liu,Qian Xu,Fan-Fan Shang,Run-Hui Zhang,Xue-Jun Jin,Fener Chen,Jung Joon Lee,Dongzhou Kang,Qing-Kun Shen,Zhe-Shan Quan
Background Ginseng possesses antitumor effects, and ginsenosides are considered to be one of its main active chemical components. Ginsenosides can further be hydrolyzed to generate secondary saponins, and 20( R )-panaxotriol is an important sapogenin of ginsenosides. We aimed to synthesize a new ginsengenin derivative from 20( R )-panaxotriol and investigate its antitumor activity in vivo and in vitro . Methods Here, 20( R )-panaxotriol was selected as a precursor and was modified into its derivatives. The new products were characterized by 1 H-NMR, 13 C-NMR and HR-MS and evaluated by molecular docking, MTT, luciferase reporter assay, western blotting, immunofluorescent staining, colony formation assay, EdU labeling and immunofluorescence, apoptosis assay, cells migration assay, transwell assay and in vivo antitumor activity assay. Results The derivative with the best antitumor activity was identified as 6,12-dihydroxy-4,4,8,10,14-pentamethyl-17-(2,6,6-trimethyltetrahydro-2 H -pyran-2-yl)hexadecahydro-1 H -cyclopenta[a]phenanthren-3-yl( tert -butoxycarbonyl)glycinate ( A11 ). The focus of this research was on the antitumor activity of the derivatives. The efficacy of the derivative A11 (IC 50 < 0.3 μM) was more than 100 times higher than that of 20( R )- panaxotriol (IC 50 > 30 μM). In addition, A11 inhibited the protein expression and nuclear accumulation of the hypoxia-inducible factor HIF-1α in HeLa cells under hypoxic conditions in a dose-dependent manner. Moreover, A11 dose-dependently inhibited the proliferation, migration, and invasion of HeLa cells, while promoting their apoptosis. Notably, the inhibition by A11 was more significant than that by 20( R )-panaxotriol ( p < 0.01) in vivo . Conclusion To our knowledge, this is the first study to report the production of derivative A11 from 20( R )-panaxotriol and its superior antitumor activity compared to its precursor. Moreover, derivative A11 can be used to further study and develop novel antitumor drugs.
研究领域:
药理学
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肿瘤学
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有机化学
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生物化学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Huajian Xu,Zhongtao Hu,Zongwei Hao,Changyue Deng,Xiaowen Pi,Binjia Zhang
The effects of amino acids with different R-groups, namely glutamic acid (GLU), glutamine (GLN), and theanine (THE), on the pasting and structure properties of corn starch (CS) were investigated. During gelatinization, GLU decreased viscosity of CS. GLN and THE increased pasting enthalpy of CS from 7.62 to 8.72 and 9.50 J/g, respectively. All starch-amino acid systems had higher storage and loss modulus. Amino acids could adhere to starch granule surface. After co-gelatinization, GLU increased short-range order degree of starch, while GLN and THE decreased. Both infrared spectroscopy and quantum computation showed that non-covalent interaction occurred between amino acids and starch. Molecular dynamics revealed that the interaction between GLU and starch weakened hydrogen bonding between starch and water, promoting cross-linking between starch chains. GLN and THE changed dihedral angle torsion of glycoside bonds, and mainly interacted with starch by electrostatic interaction (50.06 kJ/mol) and van der Waals force (50.30 kJ/mol), respectively.
研究领域:
食品科学
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化学
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材料科学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Xinman Hu,Fang-Yuan Chen,Jie Ding,Zihe Zhai,Huang Yang,Liyin Shen,Yang Zhu,Dong-Sheng Guo,Changyou Gao
The clinically restored blood supply post myocardial infarction (MI) will break the physiological and metabolic balance of the ischemic myocardial region, leading to a new injury known as myocardial ischemia/reperfusion (I/R) injury. Abundant oxygen brought by the fresh blood would trigger the damaged myocardium to produce excessive reactive oxygen species (ROS) and thereby inflammation along with the recruitment and infiltration of activated neutrophils and macrophages. Inspired by the active targeting process of inflammatory cells, a novel “neutrophil-like” nanoassembly (At@NQA) was prepared by modifying neutrophil membrane fragments onto At@QA NPs containing 1:1 (molar ratio) calixarene molecule (QA) and anti-inflammatory and anti-fibrotic atorvastatin calcium (At). Tail vein injection of the At@NQA could efficiently target the damaged myocardium, inhibit oxidative damage and inflammation, reduce myocardial fibrosis, increase myocardial troponin T (cTnT) and connexin 43 (Cx43) expression, and thus improve cardiac functions effectively.
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高影响因子
ACS Applied Energy Materials
·
2023
·
IF 6.4
Ke Wei,Jiu-Xiang Yang,Fang-Yuan Bai,Yuan-Sheng Shen,Kai Liu,Jing Liu,Li-Hua Chen,Yu Li,Bao-Lian Su
Ti3C2 MXene has been extensively studied in the field of photocatalytic hydrogen production since its discovery due to the large specific surface area and adjustable surface groups. However, the weak interaction at the interface of Ti3C2-based composites leads to a high energy barrier, which is not conducive to carrier transport. Herein, we propose the construction of a sandwich Ti3C2/R-TiO2 composite photocatalyst by in situ growing rutile TiO2 (R-TiO2) nanoneedles in Ti3C2 MXene through NaOH oxidation treatment and the microwave hydrothermal method. This in situ synthesis can make Ti3C2 MXene and TiO2 nanoneedles closely link together, which is conducive to carrier transport and material stability. The best Ti3C2/R-TiO2 composite achieves an excellent hydrogen production rate of 1.62 mmol g–1 h–1, as endowed by the 2D nanosheet-constructed sandwich structure, an increased specific surface area, and improved charge carrier separation and transport.
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高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2018
·Vol. 136
·期 7
·47068
·
IF 1.901
N.-C. Wang,L.-F. Fang,J. Wang,P. Zhang,W.-B. Wang,C.-E. Lin,L. Xiao,C. Chen,B. Zhao,H. Abdallah,H. Matsuyama,B.-K. Zhu
Carboxyl groups in the membranes usually contribute the hydrophilicity and antifouling properties. However, membrane performance including permeation and separation properties is not stable at different pH environments due to pH sensitivity of carboxyl groups. In this work, a series of membranes from random copolymers poly(vinyl chloride- r -acrylic acid) [P(VC- r -AA)] were fabricated via nonsolvent induced phase separation process. The morphology and surface properties were investigated. The pH-dependent property of carboxyl groups contained ultrafiltration membranes were studied in detail. The results indicated that pH sensitivity of these membrane was restrained, which was mainly attributed to the random sequential structure of VC and AA repeating unit in P(VC- r -AA) copolymer. The probable arched structures on the pore surface/wall at alkaline environment limited the obvious change of the pore size, which resulted in the stable permeation and separation properties at the varied pH environments. Therefore, it indicated that P(VC- r -AA) was a kind of promising membrane material which would be widely applied in water treatment field. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47068.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
·
IF 5.2
Feng Wang,Chao Li,Haohao Wang,Lu Yu,Fuming Zhang,Robert J. Linhardt
Natural amino acid polymers have been wide-used as bio-compatible emulsion stabilizers . In this study, Amphiphilic O(Phe- r -Glu)s were synthesized through the papain-catalyzed random polymerization of L -phenylalanine and L -glutamate with the major degree of polymerization value of 6–7. The polymerization yield was increased by more than 35 % using choline to reduce the energy barrier for the amino substrates to form the tetrahedral intermediate with the catalytic triad. The squalene/water emulsions stabilized by O(Phe- r -Glu) nanoparticles were pH-insensitive at pH 1–12. The investigation of mechanism found the combined effects of steric hindrance , electrostatic repulsion and the viscosity of the emulsion contributed to the stability of the emulsion droplets. Under acidic condition, the layers of O(Pho- r -Glu)s particle aggregates formed around the emulsion droplets and the higher viscosity of emulsion mainly contributed to the stability of emulsions . At basic condition, the strong electrostatic repulsion between emulsion droplets covered by the negatively charged O(Pho- r -Glu) particles and the steric hindrance contributed mainly to the prevention of the coalescence of emulsion droplets. The application of enzymatically synthesized amphiphilic peptides in emulsion, along with tunable performance through the polymerization of amino substrates, is in line with the sustainable development requirements in fields such as pharmaceuticals, food and cosmetics.
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高影响因子
Advanced Composites and Hybrid Materials
·
2022
·Vol. 5
·期 3
·2138-2153
·
IF 11.806
Ding Zhaodong,Tian Zhongjian,Ji Xingxiang,Dai Hongqi,Si Chuanling
Inspired by the hydrophobic waxy layer structure on the back of the desert beetle, a super-hydrophobic material was prepared via the sol–gel silanisation of porous cellulose membrane (CM) using triethoxysilanes with different carbon chain lengths (R-siloxane) and (CH 3 CH 2 O) 4 Ti as a catalyst in 3 min. The R-siloxane CM was both super-hydrophobic and oleophilic (water contact angle (CA) > 150°, dodecane CA ≈ 0°) and resisted liquids with surface tensions as low as 15 mN·m −1 , correlated with the length of the R-siloxane and roughness of the CM. Instructed by the water collection behavior of the desert beetle, a pristine filter CM was added on the R-siloxane filter CM to accelerate separation of Span 80–stabilised water-in-oil emulsions by generating a surface energy gradient (SEG). The composite membrane with SEG separated the emulsions with higher surfactant concentrations and provided a higher efficiency up to 99.98% compared with single-layer R-siloxane membrane. This bio-inspired catalytic one-step approach for preparing R-siloxane cellulose composite membranes can potentially replace petroleum-based products for oil purification. Graphical Abstract Bio-inspired preparation of R-siloxane composite cellulose membrane with surface energy gradient for highly efficient water-in-oil emulsion separation.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 32
·期 9
·2108830
·
IF 18.808
Hao Liu,Shuxin Wei,Huiyu Qiu,Muqing Si,Guoqing Lin,Zhenkuang Lei,Wei Lu,Lei Zhou,Tao Chen
Many living creatures have evolved to show diverse appearance color changes in response to multiple environmental stimuli for attraction, warning, or disguise in their environments. However, it is challenging to construct artificial soft polymer hydrogels with similar multi-responsive multicolor tunable behaviors, but such materials can serve as soft biomimetic skins to dramatically enhance the function of certain machines. Herein, a specially designed material structure to present an innovative class of supramolecular fluorescent polymeric hydrogels with the integrated properties of wide multi-color tunability, multi-responsiveness, self-healing, and remolding capacities is proposed. A key feature of this rational hydrogel design is that multiple fluorophores (blue (B) aggregation-induced emissive and red/green (R/G) lanthanide coordinated ones) are organized separately into different polymer chains of one single supramolecular polymer network. Consequently, the B and R/G fluorophores are engineered to be orthogonally responsive, and the fluorescence intensity of each fluorophore can be controlled independently by different external stimuli, which contribute to multi-responsive multicolor fluorescence response. Besides, the hydrogels also have satisfying self-healing and remolding capacities. All of these promising advantages together further enabled the construction of soft biomimetic color-changing skins that can help the existing robots achieve the desirable camouflaging function.
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高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2021
·Vol. 193
·期 11
·3624-3640
·
IF 2.926
Jia Dong-Xu,Peng Chen,Li Jun-Liang,Wang Fan,Liu Zhi-Qiang,Zheng Yu-Guo
ω-Transaminase (ω-TA) is an attractive biocatalyst for stereospecific preparation of amino acids and derivatives, but low catalytic efficiency and unfavorable substrate specificity hamper their industrial application. In this work, to obtain applicable ( R )-ω-TA responsible for amination of α-keto acids substrates, the reactivities of eight previously synthesized ω-TAs toward pyruvate using ( R )-α-methylbenzylamine (( R )-α-MBA) as amine donor were investigated, and Gibberella zeae TA (GzTA) with the highest ( R )-TA activity and stereoselectivity was selected as starting scaffold for engineering. Site-directed mutagenesis around enzymatic active pocket and access tunnel identified three positive mutation sites, S214A, F113L, and V60A. Kinetic analysis synchronously with molecular docking revealed that these mutations afforded desirable alleviation of steric hindrance for pyruvate and α-MBA. Furthermore, the constructed single-, double-, and triple-mutant exhibited varying degrees of improved specificities toward bulkier α-keto acids. Using 2-oxo-2-phenylacetic acid ( 1d ) as substrate, the conversion rate of triple-mutant F113L/V60A/S214A increased by 3.8-fold relative to that of wide-type GzTA. This study provided a practical engineering strategy for improving catalytic efficiency and substrate specificity of ( R )-ω-TA. The obtained experience shed light on creating more industrial ω-TAs mutants that can accommodate structurally diverse substrates. Graphical abstract
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高影响因子
危险品化学品经营许可证（带存储）