JOURNAL OF CONTROLLED RELEASE
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2023
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IF 10.8
Dan Su,Zhong Chen,Xiaobin An,Junkai Yang,Jinan Yang,Xuqiao Wang,Yang Qu,Chen Gong,Yani Chai,Xiaoying Liu,Wei Cheng,Dongyang Wang,Yan Wu,Jing Ma,Xinyue Zhao,Qin Wang,Yun Xu,Haisheng Peng,Jing Ai
The complex etiologies and mechanisms of Alzheimer's disease (AD) underscore the importance for devising multitarget drugs to achieve effective therapy. MicroRNAs (miRNAs) are capable of concurrently regulating the expression of multiple proteins by selectively targeting disease- associated genes in a sequence-specific fashion. Nonetheless, as RNA-based drugs, their stability in the circulation and capacity of traversing the blood–brain barrier (BBB) is largely compromised, thereby limiting their potential clinical applications. In this study, we formulated the nanoliposomes encapsulating polyethyleneimine (PEI)/ miR-195 complex (DPMT@PEI/ miR-195 ) that was engineered through dual modifications to contain P -aminophenyl-alpha-d-mannopyranoside (MAN) and cationic cell-penetrating peptide (TAT). DPMT@PEI/ miR-195 exhibited the enhanced BBB- and cell membrane penetrating capability. As expected, we observed that DPMT@PEI/ miR-195 administered through intravenous tail injection of produced greater effectiveness than donepezil and the same range of effect as aducanumab in alleviating the cognitive decline in 7-month-old APP/PS1 mice. Moreover, the combination treatment with DPMT@PEI/ miR-195 and donepezil effectively ameliorated the deterioration of cognition in 16-month-old APP/PS1 mice, with enhanced effects than either DPMT@PEI/ miR-195 or donepezil alone. Furthermore, DPMT@PEI/ miR-195 effectively attenuated the positive signals of Aβ, AT8, and CD68 in APP/PS1 mice without notable side effects. Our findings indicate DPMT@PEI/ miR-195 as a promising potentially new agent or approach for the prophylaxis and treatment of early and advanced stages of Alzheimer's disease.
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FOOD MICROBIOLOGY
·
2023
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IF 5.3
Hongchen Zhang,Yujun Zhai,Kewei Chen,Hui Shi
Escherichia coli O157:H7 can recover from sublethally injured (SI) state, which causes threat of foodborne illness . Adhesion plays a key role in the carriage of pathogens in food . In this study, we investigated the adhesion ability of SI and recovered E. coli O157:H7 wildtype and its three pili-deficient mutants (curli, type 1 fimbriae , and type IV pili) on six food-related surfaces. Plate counting was used to determine adhesion population after washing and oscillating the surfaces. Spinach exhibited the stronger adhesion population of E. coli O157:H7 than the other fresh produces (p < 0.05). In addition, at least one key pili dominated adhesion on these surfaces, and curli was always included. The adhesion population and contribution of different types of pili were jointly affected by surface and physiological state. This can be attributed to high hydrophobicity and positive charge density on surface and different expression levels of csgB , fimA , fimC and ppdD in SI and recovered cells. Among glucose, mannose , maltose , fructose, lactose, and sucrose , addition of 0.5% mannose could reduce adhesion of cells at all physiological states on stainless steel. Overall, this research will provide support for controlling adhesion of SI and recovered E. coli O157:H7.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Yuxiao Hu,Xueying Chen,Zhijian Tan
Ammonium sulfate and t -butanol are widely used in conventional three-phase partitioning (TPP); however, their use is not eco-friendly. In this study, a novel TPP was developed using pH-responsive deep eutectic solvents (DESs) and sugars as substitutes for t -butanol and ammonium sulfate, respectively. This TPP was further used for the separation and purification of radish ( Raphanus sativus L.) peroxidase (POD). DES-3 (dodecanoic acid: decanoic acid = 1:1) and sucrose were selected as optimal phase-forming components. Single-factor experiments were performed to investigate the factors influencing the TPP process, and three major factors (sucrose concentration, extraction time, and extraction temperature) were optimized by response surface methodology (RSM). Under the optimized conditions, the POD recovery and purification fold reached 101.66% and 11.56, respectively. Cyclic experiments confirmed the retrievability of DESs. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the novel TPP had better purification efficiency than the traditional TPP. The method proposed in this study is more efficient than conventional TPP, and it does not require the use of organic solvents and high concentration salt solutions. Therefore, this method is promising in the application of TPP.
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BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Hossain M. Zabed,Suely Akter,Mudasir A. Dar,Jamila A. Tuly,Mukesh Kumar Aswathi,Junhua Yun,Jia Li,Xianghui Qi
This study aimed to produce enhanced fermentable sugars from a novel stover system through the bioprocessing of its soluble sugars and insoluble carbohydrates. The pretreatment conditions were optimized for this high sugar-containing stover (HSS) to control inhibitor formation and obtain enhanced fermentable sugar concentrations. The optimum temperature, acid loading, and reaction time for the pretreatment were 155 °C, 0.5%, and 30 min, respectively, providing up to 97.15% sugar yield and 76.51 g/L total sugars at 10% solid-load. Sugar concentration further increased to 126.9 g/L at 20% solid-load, generating 3.89 g/L acetate, 0.92 g/L 5-hydroxymethyl furfural, 0.82 g/L furfural, and 3.75 g/L total phenolics as inhibitors. To determine the effects of soluble sugars in HSS on fermentable sugar yield and inhibitor formation, sugar-removed HSS was further studied under the optimum conditions. Although prior removal of sugars exhibited a reduction in inhibitor generation, it also decreased total fermentable sugar production to 115.45 g/L.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 20
·4935-4947
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IF 4.3
Chen Yiyi,Shen Yanghong,Wang Haifeng,Zhang Jinzhi,Zhu Junli
Due to universal contamination and synergistic toxicity of multiple mycotoxins in foodstuff, reliable and high-throughput detection methods for multiple mycotoxins are urgently needed in corn products. In this study, a novel dual-channel immunochromatographic assay (ICA) based on improved up-conversion nanoparticles (IUCNPs) was developed for rapidly detecting aflatoxin B1 (AFB1) and zearalenone (ZEN). The synthesized IUCNPs doped by 30% Lu 3+ showed a larger size, more regular structure, and brighter fluorescence intensity than conventional UCNPs. The limits of detection (LODs) of single-channel ICA test strips for AFB1 and ZEN detection were 0.01 and 0.1 ng/mL, respectively. After the optimization, the dual-channel ICA of AFB1 and ZEN in 10 min was conducted, resulting in low detection limits of 0.025 and 0.1 ng/mL, respectively. Moreover, the built assay was revealed to be highly specific for six other food-contaminated mycotoxins, and exhibited excellent accuracy, with corresponding R 2 of 0.9931 and 0.9982 in calibration curves, respectively. Long-term storage experiments indicated that the dual-channel test strips had superior stability and precision. The LODs of AFB1 and ZEN in spiked maize were 0.025 and 0.25 μg/kg, demonstrating great sensitivity and matrix tolerance. Furthermore, the IUNCP-ICA was validated by high-performance liquid chromatography (HPLC) analyses, and a satisfactory consistency was obtained in 15 natural maize samples. Thus, the IUCNPs-ICA proposed in this work realized rapid and sensitive detection of AFB1 and ZEN, providing broad application potential in on-site screening for multiple mycotoxins in agricultural products. Graphical abstract
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International Journal of Electrochemical Science
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2023
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IF 1.5
Hong Liu,Yichen Deng,Jinxiang Mao,Minmin Chen,Jinxia Hu,Zhicheng Ju,Zheng Xing,Xichuan Cao
Nitrogen-doped graphene (NG) materials were successfully synthesized in one step by the restricted template method. Specifically, the use of different nitrogen sources and carbon sources makes the content of various nitrogen doping structures in NGs different and yields a higher nitrogen doping amount. In the lithium insertion process, nitrogen doping provides more active sites, which effectively improve the specific capacity. As anodes for lithium-ion batteries (LIBs), NGs exhibit excellent electrochemical performance with high reversible specific capacity, good cycle stability, and excellent rate performance.
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Biosensors-Basel
·
2023
·Vol. 13
·期 6
·661
·
IF 5.4
Tongtong Zhang,Shuai Xu,Xingyu Lin,Jiyang Liu,Kai Wang
Herein we report a highly specific electrochemical aptasenseor for AFB1 determination based on AFB1-controlled diffusion of redox probe (Ru(NH3)63+) through nanochannels of AFB1-specific aptamer functionalized VMSF. A high density of silanol groups on the inner surface confers VMSF with cationic permselectivity, enabling electrostatic preconcentration of Ru(NH3)63+and producing amplified electrochemical signals. Upon the addition of AFB1, the specific interaction between the aptamer and AFB1 occurs and generates steric hindrance effect on the access of Ru(NH3)63+, finally resulting in the reduced electrochemical responses and allowing the quantitative determination of AFB1. The proposed electrochemical aptasensor shows excellent detection performance in the range of 3 pg/mL to 3 μg/mL with a low detection limit of 2.3 pg/mL for AFB1 detection. Practical analysis of AFB1 in peanut and corn samples is also accomplished with satisfactory results by our fabricated electrochemical aptasensor.Keywords:vertically aligned mesoporous silica films;electrochemical aptasensor;aflatoxin B1
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CHEMICAL SENSES
·
2023
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IF 3.5
He Yue,Chen Jianshe,Shi Weiyao,Shi Jingang,Ma Tian,Wang Xinmiao
While accumulating evidence implied the involvement of retro-nasal sensation in the consumption of nonvolatile taste compounds, it is still unclear whether it was caused by the taste compounds themselves, and if so, how can they migrate from the oral to nasal cavity. At first, we proposed aerosol particles as an alternative oral–nasal mass transfer mechanism. The high-speed camera approved that aerosol particles could be generated by the typical oral and pharynx actions during food oral processing; while the narrow-band imaging of nasal cleft and mass spectrometry of nostril-exhaled air approved the migration of aerosol within the oral–nasal route. Then, the “smelling” of taste compounds within the aerosol particles was testified. The four-alternative forced choices (4AFC) approved that the potential volatile residues or contaminants within the headspace air of pure taste solution cannot arouse significant smell, while the taste compounds embedded in the in vitro prepared aerosol particles can be “smelled” via the ortho route. The “smell” of sucrose is very different from its taste and the “smell” of quinine, implying its actual olfaction. The sweetness intensity of sucrose solution was also reduced when the volunteers’ noses were clipped, indicating the involvement of retro-nasal sensation during its drinking. At last, the efficiency of aerosol as a mechanism of oral–nasal mass transfer was demonstrated to be comparable with the volatile molecules under the experimental condition, giving it the potential to be a substantial and unique source of retro-nasal sensation during food oral processing.
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APPLIED CLAY SCIENCE
·
2023
·
IF 5.6
Ting Zhang,Ge Bai,Nan Cai,Yongqian Lei,Pengran Guo,Jingwei Xu
Since the outbreak of COVID-19, the extensive use of epidemic control drugs such as Arbidol (ARB) and Acyclovir (ACV) has significantly increased and the environmental pollution caused by these residues has attracted increasing attention. In this article, a novel montmorillonite graphene-like carbon hybrid Fe 3 O 4 compound (Mt/GH/Fe 3 O 4 ) as particle electrode catalyst was prepared and applied in three-dimensional-electro-Fenton (3D-EF) for the ARB and ACV degradation. The structure and composition of the compound catalyst were characterized. The degradation efficiency of targets was significantly increased in the 3D-EF degradation system and even more thorough for the degradation of targets, compared with simple 2D, 3D, and EF catalytic degradation systems. The degradation rate of antiviral drugs can reach >90% in 5 min with excellent stability. The research demonstrated that the clay mineral with a special structure loaded with graphene-like carbon and Fe 3 O 4 had supplied an ideal reaction medium for the electro-Fenton reaction. Mt./GH/Fe 3 O 4 -based 3D-EF degradation system would have promising applications in the degradation and removal of antiviral drug residuals in the environment.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2023
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IF 6.2
Shasha Wang,Qing Wang,Wuxin Liu,Yan Wang,Yuli Wei,Shaohua Luo,Pengqing Hou,Yahui Zhang,Shengxue Yan,Xin Liu
Due to the advantages of abundant precursors, simple preparation, and environmental friendliness, nitrogen-doped carbon is considered one of the most potential anodes for potassium ion batteries (PIBs). Here, nitrogen-doped carbon materials (NC-2) were prepared by using secondary exfoliated g-C 3 N 4 as a precursor, combined with sucrose, and subjected to hydrothermal and high-temperature pyrolysis. NC-2 is characterized by high nitrogen content, porous layered structure, and large interlayer spacing. NC-2 used as anode of PIBs shows a high reversible capacity of 261.07 mAh g −1 at 0.1 A g −1 and an impressive rate performance of 153.36 mAh g −1 at 2 A g −1 . The impressive performance of NC-2 can be originated from the porous layered structure and enlarged interlayer spacing promotes the intercalation/deintercalation and the diffusion of K ions , and the defective carbon structure formed after nitrogen doping can supply abundant active sites for K ions. The results show that NC-2 has great potential in the field of PIBs anode.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
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IF 8.4
Shuhuai Li,Yan Deng,Chaohai Pang,Xionghui Ma,Yuwei Wu,Mingyue Wang,Jianping Li,Zhi Xu,Lianming Zhang
The performance of an electrochemiluminescent reagent directly affects the performance of its corresponding electrochemiluminescent (ECL) sensor. In this study, Fe/Zn-benzene-1,3,5-tricarboxylic acid metal–organic framework@C-dots (Fe/Zn-BTC@C-dots), which consist of a nano-loaded carbon quantum dot material with an excellent luminescence performance, were synthesised for the first time. Subsequently, they were used to modify the surface of a glassy carbon electrode, and combined with a single-chain thiamethoxam DNA aptamer to construct a biosensor. A novel method for the detection of thiamethoxam was established based on control of the excellent luminescence signal of the Fe/Zn-BTC@C-dots by specific recognition of the aptamer by the target molecule (a signal switch). Upon capturing thiamethoxam, the electrochemical luminescence intensity of the sensor was quenched. The ability of Fe/Zn-BTC to effectively catalyse and amplify the electrochemical luminescence of the C-dots significantly improved the sensitivity of the sensor, while the specific recognition of the target by the aptamer imparted the sensor with an excellent selection ability. Such an advanced approach is promising for the detection of thiamethoxam, with a linear range of 5–5000 × 10 −11 mol/L and a sensitivity of 9.81 × 10 −12 mol/L being achieved. Moreover, the sensor exhibited recovery rates of 90.5–109.3% upon the detection of thiamethoxam in actual samples.
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RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2023
·Vol. 37
·期 18
·e9603
·
IF 2
Weiyang Zhang,Jing Cai,Wenqing Gao,Renlu Han,Haixing Wang,Yanfei Wu,Jiawei Wu,Yong Wu,Chenlu Wang,Keqi Tang,Jiancheng Yu
Rationale In the field of separation science, ion mobility spectrometry (IMS) plays an important role as an analytical tool. However, the lack of sufficient structural resolution is a common problem in qualitative and quantitative analysis using IMS. A method is needed to solve the problem of overlapping peaks caused by insufficient resolution. Methods The method uses multiple strategies to more effectively use population information to balance exploration and exploitation capabilities, prevent local optimization, accurately resolve overlapping peaks, quickly obtain optimal spectral peak model coefficients, and accurately identify compounds. Results Multistrategy JAYA algorithm's (MSJAYA) performance is compared with improved particle swarm optimization (IPSO), dynamic inertia weight particle swarm optimization (DIWPSO), and multiobjective dynamic teaching-learning-based optimization (MDTLBO). The analysis shows that MSJAYA's maximum separation error is within 0.6%, a level of accuracy not guaranteed by the other algorithms. In addition, the separation error fluctuates within a much smaller range, demonstrating MSJAYA's superior robustness. Conclusions Compared with other overlapping peak separation algorithms, MSJAYA is more applicable because no special parameters are used. The method allows fast deconvolution analysis of strong overlapping peaks with multiple components, which greatly improves the resolution of IMS.
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危险品化学品经营许可证（带存储）