HISTOCHEMISTRY AND CELL BIOLOGY
·
2025
·Vol. 163
·期 1
·1-15
·
IF 2.1
Zhong Wen-Jing,Xiong Jian-Bing,Zhang Chen-Yu,Jin Ling,Yang Nan-Shi-Yu,Sha Han-Xi,Liu Yu-Biao,Duan Jia-Xi,Guan Cha-Xiang,Zhou Yong,Su Feng
Pulmonary fibrosis (PF) is an insidious, progressive, and fatal age-associated disease that occurs primarily in older adults and has a poor prognosis. Alveolar epithelial cell (AEC) senescence is the critical pathological mechanism of PF. The accumulation of oxygen radicals, commonly referred to as reactive oxygen species (ROS), strongly contributes to cellular senescence. The triggering receptor expressed on myeloid cells-1 (TREM-1) is a pattern recognition receptor. Triggering via TREM-1 results in ROS, leading to the amplification of inflammation. However, whether TREM-1 is involved in PF by inducing oxidative stress to exacerbate AEC senescence remains unclear. We first observed that blockade of TREM-1 during the fibrotic phase attenuated bleomycin (BLM)-induced PF in mice, with decreased expression of senescence-related proteins, including p16, p21, p53, and γ-H2AX, in the lung tissue. Moreover, TREM-1 blockade during the fibrosis stage restored antioxidant levels by increasing the percentage of Nrf2- and HO-1-positive cells in mice with PF. Notably, TREM-1 was highly expressed in surfactant-associated protein (SPC)-positive AECs in mice with PF. In vitro, blocking TREM-1 activated Nrf2 antioxidant signaling, thereby decreasing intracellular ROS levels and diminishing BLM-induced senescence in AECs. Furthermore, inhibition of Nrf2/HO-1 partially counteracted the anti-senescence effect of blocking TREM-1 in BLM-treated AECs. In this study, we reported that TREM-1 stimulated the senescence of AECs, induced ROS and exacerbated PF. We also provide compelling evidence suggesting that the Nrf2/HO-1 signaling pathway underpins TREM-1-triggered senescence. Therefore, our findings provide new insights into the molecular mechanisms associated with TREM-1 and AEC senescence in the pathogenesis of PF.
研究领域:
医学
·
分子生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 15
·9316-9320
·
IF 3.108
Li-Long Jiang,Xiangzhou Zeng,Mengkai Li,Man-Qing Wang,Tong-Yu Su,Xiao-Chun Tian,Jing Tang
We synthesized a HKUST-1 metal–organic framework (MOF) on indium tin oxide (ITO) using an electrodeposited Cu film. The electrodeposited Cu/ITO was used as a source of Cu ions, which were linked with benzene-1,3,5-tricarboxylic acid on the ITO surface to form HKUST-1. Synthesized HKUST-1/ITO was characterized by X-ray diffraction (XRD), Raman spectroscopy and scanning electron microscopy (SEM), which showed that the morphology of the HKUST-1 framework was nearly octahedral. Our results also revealed that the growth of HKUST-1 depended on the potential and synthesis time used. This method can be applied to fabricate ordered patterns of HKUST-1 on ITO substrates, offering a new way to grow MOFs on many other kinds of conductive substrates.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
NUCLEIC ACIDS RESEARCH
·
2022
·Vol. 50
·期 17
·9858-9872
·
IF 19.16
You Xue-Jiao,Zhang Shan,Chen Juan-Juan,Tang Feng,He Jingang,Wang Jie,Qi Chu-Bo,Feng Yu-Qi,Yuan Bi-Feng
RNA molecules harbor diverse modifications that play important regulatory roles in a variety of biological processes. Over 150 modifications have been identified in RNA molecules. N6-methyladenosine (m6A) and 1-methyladenosine (m1A) are prevalent modifications occurring in various RNA species of mammals. Apart from the single methylation of adenosine (m6A and m1A), dual methylation modification occurring in the nucleobase of adenosine, such as N6,N6-dimethyladenosine (m6,6A), also has been reported to be present in RNA of mammals. Whether there are other forms of dual methylation modification occurring in the nucleobase of adenosine other than m6,6A remains elusive. Here, we reported the existence of a novel adenosine dual methylation modification, i.e. 1,N6-dimethyladenosine (m1,6A), in tRNAs of living organisms. We confirmed that m1,6A is located at position 58 of tRNAs and is prevalent in mammalian cells and tissues. The measured level of m1,6A ranged from 0.0049% to 0.047% in tRNAs. Furthermore, we demonstrated that TRMT6/61A could catalyze the formation of m1,6A in tRNAs and m1,6A could be demethylated by ALKBH3. Collectively, the discovery of m1,6A expands the diversity of RNA modifications and may elicit a new tRNA modification-mediated gene regulation pathway.
研究领域:
分子生物学
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表观遗传学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Shuqi Guo,Qiang Zhao,Hongbo Hu,Wei Wang,Muhammad Bilal,Qiang Fei,Xuehong Zhang
Phenazine-1-carboxylic acid (PCA) secreted by Pseudomonas chlororaphis has been commercialized and widely employed as an antifungal pesticide. However, it displays potential hazards to nontarget microorganisms and the environment. Although the PCA degradation characteristics have received extensive attention, the biodegradation efficiency is still insufficient to address the environmental risks. In this study, an engineered Pseudomonas capable of degrading PCA was constructed by introducing heterologous PCA 1,2-dioxygenase (PcaA1A2A3A4). By integrating the PCA degradation module in the chemical mutagenesis mutant P3, 7.94 g/L PCA can be degraded in 60 h, which exhibited the highest PCA degradation efficiency to date and was 35.4-fold higher than that of the PCA natural degraders. Additionally, PCA was converted to 1-methoxyphenazine through structure modification by introducing the functional enzymes PhzSPa and PhzMLa, which has good antifungal activity and environmental compatibility. This work demonstrates new possibilities for developing PCA-derived biopesticides and enables targeted control of the impact of PCA in diverse environments.
研究领域:
环境科学
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微生物学
·
生物技术
·
化学工程
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DOI
PubMed
高影响因子
PLoS One
·
2013
·Vol. 8
·期 8
·e70565
·
IF 3.73
Luyan Guo,Yanyan Xing,Rui Pan,Mingliang Jiang,Zheng Gong,Liqing Lin,Junbing Wang,Guoyin Xiong,Jun Dong
Curcumin is a molecule found in turmeric root that has anti-inflammatory, antioxidant, and anti-tumor properties and has been widely used as both an herbal drug and a food additive to treat or prevent neurodegenerative diseases. To explore whether curcumin is able to ameliorate HIV-1-associated neurotoxicity, we treated a murine microglial cell line (N9) and primary rat cortical neurons with curcumin in the presence or absence of neurotoxic HIV-1 gp120 (V3 loop) protein. We found that HIV-1 gp120 profoundly induced N9 cells to produce reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1). HIV-1 gp120 also induced apoptosis of primary rat cortical neurons. Curcumin exerted a powerful inhibitory effect against HIV-1 gp120-induced neuronal damage, reducing the production of ROS, TNF-α and MCP-1 by N9 cells and inhibiting apoptosis of primary rat cortical neurons. Curcumin may exert its biological activities through inhibition of the delayed rectification and transient outward potassium (K + ) current, as curcumin effectively reduced HIV-1 gp120-mediated elevation of the delayed rectification and transient outward K + channel current in neurons. We conclude that HIV-1 gp120 increases ROS, TNF-α and MCP-1 production in microglia, and induces cortical neuron apoptosis by affecting the delayed rectification and transient outward K + channel current. Curcumin reduces production of ROS and inflammatory mediators in HIV-1-gp120-stimulated microglia, and protects cortical neurons against HIV-1-mediated apoptosis, most likely through inhibition of HIV-1 gp120-induced elevation of the delayed rectification and transient outward K + current.
研究领域:
神经科学
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免疫学
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药理学
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DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 11
·期 1
·192-197
·
IF 3.119
Qiaoe Wang,Meiling Lian,Xiaowen Zhu,Xu Chen
The copper-based MOF, HKUST-1 has been applied for humidity sensing owing to hydrophilic ligands and open metal sites which are suitable for sensitively detecting moisture. However, most of the research on the sensor HKUST-1 focuses on the role of the central metal. There are few reports on the morphology–activity relationship of HKUST-1. In this work, we synthesized two kinds of HKUST-1 including octahedral structures and ultrathin nanosheets, and systematically studied the performance of moisture sensing. Compared to HKUST-1 octahedra, HKUST-1 nanosheets showed lower and wider detectable humidity range, achieving a fast response. Starting from the exposed hydrophilic functional groups of HKUST-1 nanosheets, we have revealed that hydrophilic ligands play an important role in improving the adsorption capacity during the adsorption process. In addition, ultra-thin HKUST-1 nanosheets act as an excellent mass transfer medium, accelerating proton transfer and water molecule movement. To further improve the performance of the HKUST-1 humidity sensor, black phosphorus quantum dots (BPQDs) with a high surface reactivity were used to build a composite sensing platform. The excellent proton transfer capability of BPQDs leads to one order of magnitude improvement in the sensitivity of the BPQDs/HKUST-1 systems compared to HKUST-1 only.
研究领域:
材料科学
·
化学工程
·
传感器技术
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DOI
高影响因子
Reaction Chemistry & Engineering
·
2025
·
IF 3.1
Xueqian Zhang,Jiasong Yan,Shuai Yuan,Shanqing Yu,Qingyu Liu,Xikai Wang,Rui Li
Whether cycloalkanes undergo six- or five-membered ring-opening reaction pathways remains controversial. In this work, the catalytic cracking reactions of cyclohexene and 1-methylcyclopentene, as model compounds, were investigated in a pulse microreactor. The characteristics of the reactions of the two model compounds were compared in terms of isomerization, cracking, and hydrogen transfer reaction, and the reasons for the differences in their catalytic reactions were analyzed in combination with thermodynamic and molecular simulation calculations. The results have shown that the catalytic cracking product distributions of cyclohexene and 1-methylcyclopentene were similar owing to their easy mutual isomerization; the five-membered ring-opening cracking pathway was more thermodynamically advantageous than that of six-membered ring-opening cracking, and therefore, the yield of the ring-opening cracking product of 1-methylcyclopentene was comparatively higher; the main products of the hydrogen transfer reaction of the two compounds were alkanes and aromatic hydrocarbons. By comparing the product distributions and reaction activation energies, the ring-opening reaction pathways were clarified, and the catalytic cracking reaction networks of cyclohexene and 1-methylcyclopentene were established.
研究领域:
化学
·
催化反应
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DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2021
·Vol. 299
·期 9
·1459-1468
·
IF 1.931
Guan Peiwen,Ren Cong,Shan Houchao,Cai Di,Zhao Peimian,Ma Dongze,Qin Peiyong,Li Shufeng,Si Zhihao
To meet the increasing requirements toward biobutanol, pervaporation membrane with high separation performance needs to be developed. In this work, a hydrophobic MAF-6 (RHO-[Zn(eim) 2 ]) was synthesized and its adsorption properties for 1-butanol was systematically studied, which are supposed to benefit the separation performance of polydimethylsiloxane (PDMS) membrane. The hydrophobic MAF-6 particles were therefore doped into PDMS to fabricate mixed matrix membrane. The morphology, chemical structure, and other properties of particles and hybrid membrane were fully studied by SEM, PXRD, FT-IR, TGA, and BET. Results demonstrate that the hydrophobicity of PDMS membrane is improved with the water contact angle increasing from 112.7° to 118.1° after being doped with MAF-6, as well as enhanced adsorption ability toward 1-butanol. More importantly, when doping MAF-6 into PDMS layer, the enhancements of 23.30% and 41.93% are separately observed in the separation factor and total flux to separate 1.5 wt% 1-butanol solution, compared with the PDMS membrane. In addition, the heat energy consumption of pervaporation process is decreased from 4.27 to 3.71 kJ g −1 via the doping of MAF-6. Overall, this work confirms that the hydrophobic particle such as MAF-6 is not only conductive to enhance the separation performance of PDMS membrane but also helpful to decrease the energy consumption.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
·
2023
·
IF 5.2
Pei Li,Zhishuang Yang,Ting Lei,Yujie Dai,Yang Zhou,Dekang Zhu,Hongyan Luo
Objectives: To elucidate the role of a novel carbapenem-hydrolysing class D β-lactamase (RAD-1) from Riemerella anatipestifer. Methods: We applied WGS and bioinformatic analysis to screen putative β-lactamase genes in R. anatipestifer SCVM0004. A putative class D β-lactamase gene was cloned into pET24a and transferred into Escherichia coli BL21 (DE3) for antibiotic susceptibility determination and protein purification. Meanwhile, the purified native protein was used to determine the enzymatic activities. Results: A class D β-lactamase, RAD-1, was identified in the genome of R. anatipestifer SCVM0004. It was distinct from all characterized class D β-lactamases (≤42% amino acid sequence identity). Searching in GenBank showed that blaRAD-1 was widely disseminated among R. anatipestifer. Genomic environment analysis indicated that the chromosomal structures of blaRAD-1-located regions were relatively conserved. Expression of RAD-1 in E. coli results in elevated MICs for various β-lactam antibiotics, including penicillins, extended-spectrum cephalosporins, a monobactam and carbapenems. Moreover, kinetic analysis of purified RAD-1 revealed: (i) high-level activity against penicillins; (ii) highest affinity for carbapenems; (iii) moderate hydrolysis of extended-spectrum cephalosporins and a monobactam; and (iv) no activity for oxacillin and cefoxitin. Conclusions: This study identified a novel chromosomally located class D carbapenemase RAD-1 (Bush-Jacoby functional group 2def) in R. anatipestifer SCVM0004. Moreover, bioinformatic analysis confirmed that the RAD-1 was widely prevalent and conserved in R. anatipestifer.
研究领域:
微生物学
·
基因组学
·
抗菌素耐药性
·
生物信息学
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DOI
PubMed
高影响因子
MOLECULAR PHARMACOLOGY
·
2025
·
IF 3
Shixin Li,Jingyan Wei,Shaofen Zhou,Shuaiqi Ma,Peihang Jiang,Jian He
The rise of drug-resistant influenza A virus (IAV) strains and severe lung injury caused by excessive inflammation highlight the need for new antivirals that combine antiviral and immunomodulatory effects. Adamantane, an established anti-IAV agent, targets the viral M2 matrix protein, but widespread mutations have limited its clinical utility. Previously, we identified that 2,5-dihydroxybenzylalcohol and its derivatives possess anti-IAV activity. In this study, we synthesized a series of adamantane-2,5-dihydroxy-benzylalcohol derivatives via reductive amination. Among these, Amt-1 demonstrated strong antiviral activity against both amantadine-sensitive (H1N1 PR8) and clinical isolates IAV strains (-log IC 50 =6.12–6.51 M). Mechanistic analyses, including immunofluorescence, ITC, qPCR, ELISA, luciferase assays, and Western blotting, revealed that Amt-1 not only interacts with the peptide segment of M2 ion channel protein (-log Kd = 5.08 M) but also activates Nrf2, leading to increased HO-1 expression and reduced production of pro-inflammatory cytokines IL-6, IL-1β, and TNF-α. Additionally, Amt-1 inhibited IAV-induced NF-κB activation, thereby suppressing viral replication. In vivo , Amt-1 reduced lung viral titers and mitigated histopathological damage in mice, surpassing amantadine in controlling inflammation and increased survival to 33%. In summary, Amt-1 is the first adamantane derivative shown to exert anti-IAV effects by modulating Nrf2/HO-1-mediated anti-inflammatory pathways and NF-κB-driven antiviral responses, while retaining M2 ion channel inhibition. This host-directed mechanism addresses drug resistance and limits immunopathology, making Amt-1 a promising therapeutic candidate for resistant influenza infections.
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PubMed
高影响因子
JOURNAL OF BIOLOGICAL CHEMISTRY
·
2020
·Vol. 295
·期 19
·6425-6446
·
IF 4.238
Chengmeng Jin,Xiaobing Zhu,Hao Wu,Yuqi Wang,Xun Hu
Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the PGK1-catalyzed forward reaction. The Km values for 1,3-bisphosphoglyceric acid (1,3-BPG, the forward reaction substrate) were 4.36 μm (yeast PGK1) and 6.86 μm (human PKG1). The Km values for 3-phosphoglycerate (3-PG, the reverse reaction substrate and a serine precursor) were 146 μm (yeast PGK1) and 186 μm (human PGK1). The Vmax of the forward reaction was about 3.5- and 5.8-fold higher than that of the reverse reaction for the human and yeast enzymes, respectively. Consistently, the intracellular steady-state concentrations of 3-PG were between 180 and 550 μm in cancer cells, providing a basis for glycolysis to shuttle 3-PG to the serine synthesis pathway. Using siRNA-mediated PGK1-specific knockdown in five cancer cell lines derived from different tissues, along with titration of PGK1 in a cell-free glycolysis system, we found that the perturbation of PGK1 had no effect or only marginal effects on the glucose consumption and lactate generation. The PGK1 knockdown increased the concentrations of fructose 1,6-bisphosphate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, and 1,3-BPG in nearly equal proportions, controlled by the kinetic and thermodynamic states of glycolysis. We conclude that perturbation of PGK1 in cancer cells insignificantly affects the conversion of glucose to lactate in glycolysis.
研究领域:
生物化学
·
癌症生物学
·
细胞代谢
·
酶学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
C. Shen,Y.J. Wang,J.H. Xu,G.S. Luo
Porous glass supported with titanium silicalite (TS-1) particles were successfully prepared and used for oxidative desulfurization (ODS) of dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT). Uniform-sized TS-1 particles were homogeneously dispersed on the surface of the porous glass beads. Effects of reaction duration, catalyst content, initial concentration, oxidant amount, and temperature on conversions were investigated. The prepared catalysts showed excellent catalytic performance. The optimal mole ratio of oxidant-to-DBT was 10 or 15. Complete conversion was obtained within 3 min at 343 K using catalyst with Ti content of 0.507 wt.%. Compared with other studies, the space–time yield was increased by one order of magnitude without any addition of solvent or metal nanoparticles. Experimental results and computer simulations revealed that the coordinated state of Ti species has a key role in the catalytic activity. Spent catalyst would be regenerated by heating at high temperature, no decrease was observed in catalytic activity after three cycles.
研究领域:
化学工程
·
催化材料
·
环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）