LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Xi Cao,Linmeng Liao,Fengqin Feng
Lipase catalysis has become the most promising technique for enrichment of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) due to its mild and green conditions. In this study, Trichosporon sp. F1-2 was newly isolated from oil-contaminated sludge, and its extracellular lipase was purified using ammonium sulfate precipitation and DEAE-Sepharose chromatography. The molecular weight of the lipase was estimated to be 32.6 kDa. The optimal temperature and pH for lipase activity were 50 °C and 8.0, respectively, and the optimal storage conditions were found to be temperatures below 45 °C and pH 7.0–9.0. The lipase did not require metal ions as cofactors and was significantly inhibited by Zn 2+ . It was stable in various organic solvents with different log P values, and n-hexane, toluene, dichloromethane, diethyl ether and glycerol even enhanced its activity. It had sn -1,3 regioselectivity and the highest activity towards a medium-chain fatty acid (C8) ester. This study demonstrated the lipase has good potential as a catalyst in enrichment of EPA and DHA in fish oil. The content of EPA and DHA reached 22.9 and 25.4 mol%, respectively, using 100 U of the lipase within 24 h, and the enrichment efficiency of DHA was higher than that of EPA.
研究领域:
生物技术
·
酶学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 5
·2033-2042
·
IF 4.813
Wang Xiao,Xu Na,Li Qinglin,Chen Shengqi,Cheng Hui,Yang Mo,Jiang Ting,Chu Jun,Ma Xiaojing,Yin Dengke
Liver cancer, one of the most common types of cancer in the world, is the second leading cause of death for cancer patients. For liver cancer, there is an urgent need for an effective treatment with no or less toxic side effects. Lactonic sophorolipids (LSL), as a potential anticancer drug, has attracted wide attention of pharmaceutical researchers with its good biological activities. The effects of LSL and cell death inhibitors were measured by MTT test on HepG2 cells. Meanwhile, the morphology of the cells was observed under a microscope. The apoptosis rate was detected by flow cytometry, and the expression levels of enzyme activity of Caspase-3 and Caspase-9 were measured by detection kits. Meanwhile, mRNA levels of Apaf-1 , Caspase-3 , Bax , and Bcl-2 were measured by quantitative real-time RT-PCR; protein levels of Caspase-3, Cleaved Caspase-3, Bax, and Bcl-2 were measured by western blot. LSL can inhibit the proliferation of cells, and it is possible to induce apoptosis in cells. The HepG2 cells with LSL co-culture exhibited typical apoptotic morphology, and the expression levels of enzyme activity of Caspase-3 and Caspase-9 increased ( P < 0.05). We also found that LSL increases cell apoptosis rate and regulates the expression of genes and proteins associated with apoptosis through the Caspase-3 pathway. These results indicate that LSL may be one of the potential drug candidates to inhibit the proliferation and induce apoptosis in HepG2 cells.
Key points
• LSL, which is of good biological activities such as anti-bacterium, virus elimination, and inflammatory response elimination, has been firstly used to intervene in vitro to investigate its effect on HepG2 cell proliferation. • LSL can inhibit the proliferation of cells, and it is possible to induce apoptosis in HepG2 cells through the Caspase-3 pathway. • The mechanism of LSL action on HepG2 cell proliferation was firstly also discussed, which provides a certain experimental reference for the clinical treatment of liver cancer. Graphical abstract
研究领域:
肿瘤学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2021
·Vol. 13
·期 9
·8115-8121
·
IF 4.987
Ren Dezhang,Jiang Xuelei,Zhang Nahui,Duo Jia,Norinaga Koyo,Huo Zhibao
Production of pyridine derivatives from bio-based feedstocks instead of fossil fuels is a promising strategy. The development of effective heterogeneous catalysts and mild reaction systems for pyridinization of renewable biomass derivatives remains a huge challenge. In this article, a direct and environment-friendly method was reported to produce 3-hydroxypyridine from bio-based furfural in water over a Raney Fe catalyst. Raney Fe exhibited an efficient catalytic performance for activation of the aldehyde group of furfural and afforded an 18.2% yield of 3-hydroxypyridine at 120 °C with ammonia as N source. The polymerization of furfural on the catalyst in hot water was the main cause of deactivation, which was verified by the scanning electron microscope (SEM), X-ray diffraction (XRD), and N 2 adsorption and desorption analysis. A possible mechanism for this reaction was also proposed. This study provides a new way for academia and industry to realize the synthesis of nitrogen-containing chemicals from biomass and its derivatives.
研究领域:
催化化学
·
绿色化学
·
可再生资源转化
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL PHYSICS LETTERS
·
2022
·
IF 2.719
Zixiang Guo,Xue Qin,Yulong Xie,Chanrong Lei,Tianyu Wei,Yuzhe Zhang
Nanoparticle LiTi 2 (PO 4 ) 3 was prepared by solvothermal and calcination methods. LiTi 2 (PO 4 ) 3 -xh(x = 5, 7, 9, 13) was successfully synthesized under different calcination times in nitrogen atmosphere, and LiTi 2 (PO 4 ) 3 –7h showed excellent rate and electrochemical performance. It delivered discharge capacities of 141.2, 119.9, 109.8, 98.2, 87.3, and 76.7 mAh·g −1 at rates of 0.5, 1, 2, 5, 10, and 20C, respectively. In particular, its initial discharge capacity can reach 123.3 mAh·g −1 at the high rate of 10C. Hence, these superior properties demonstrate that LiTi 2 (PO 4 ) 3 –7h has great application advantages as a cathode for lithium-ion batteries.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 31
·期 21
·215605
·
IF 3.551
Yu-Wei Liu,Shao-Hong Guo,Si-Qi You,Chun-Yi Sun,Xin-Long Wang,Liang Zhao,Zhong-Min Su
Halide perovskites have been employed as photocatalysts for CO2 photoreduction due to their excellent optical properties and unique electronic structure. However, their photocatalytic performance is relatively poor. Herein, we demonstrate a new strategy with Mn-doped CsPb(Br/Cl)3 mixed-halide perovskites as catalysts to enhance the efficiency of CO2 photoreduction. By tuning the content of Mn, a series of CsPb(Br/Cl)3:Mn perovskites are obtained and show high efficiency in CO2 conversion to CO and CH4. For the optimum catalyst sample, especially, the yields of CO and CH4 reach 1917 μmol g−1 and 82 μmol g−1 which are 14.2 and 1.4 times higher than those of CsPbBr3. This work provides new insights into improving the reactivity of perovskites in CO2 photoreduction.
研究领域:
材料科学
·
催化化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2023
·Vol. 13
·期 24
·7046-7058
·
IF 5
Wei Jin,Dekang Liu,Liyuan Zhang,Qian Sun,Yishan Wang,Enzhou Liu,Xiaoyun Hu,Hui Miao
Titanium dioxide (TiO2), a conventional n-type semiconductor, was widely used in photocatalysis, electrocatalysis and photoelectrocatalysis due to its good UV absorption and stable physical and chemical properties. However, its wide band gap and low oxygen reaction (OER) activity limited its application in photoelectrochemical (PEC) water splitting. In this work, we successfully constructed type-II Sb2(S,Se)3@TiO2 core–shell heterojunctions. Antimony sulfide selenide (Sb2(S,Se)3) was a quasi-one-dimensional light-absorbing material with an adjustable band gap (1.1–1.8 eV), which broadened the TiO2 light absorption range and effectively promoted the photogenerated carrier separation, transportation and utilization. Of particular note, novel Sb2(S,Se)3 nanospheres (NSPs) (ca. 69 nm) were in situ grown inside the tubes attributed to the unique space-confinement effect of TiO2 nanotubes (NTs). The IPCE value for Sb2(S,Se)3@TiO2 at 734 nm was 10.808% compared to 0.030% for TiO2. The separation efficiency and injection efficiency increased from 2.48% and 31.62% to 4.90% and 36.48%, respectively. The onset potential was moved negatively by 60 mV, and the maximum photocurrent density of 1.53 mA cm−2 at 1.23 V vs. RHE was 13.9 times higher than that of TiO2 (0.11 mA cm−2). This work provided a new idea for the application of TiO2 in the field of PEC water splitting.
研究领域:
材料科学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
Biomedical Materials
·
2025
·Vol. 20
·期 2
·025030
·
IF 3.7
Juan Huang,Yanhui Li,Mengru Zhu,Jigang Luo,Zhuoyue Song,Shijie Li,Tao Liu,Chunzhi Tang,Nenggui Xu,Shihui Liu
Neuropathic pain is a worldwide problem that causes physical and psychological pain to many patients. 3-acetylaconitine (AAC) is a kind of non-narcotic analgesic with long-lasting action, non-tolerant and non-addiction. However, it has some cardiac toxicity and can easily cause toxic organ damage. To solve these problems, dissolvable microneedle (MN) patches were prepared and delivered subcutaneously through the skin barrier. The results showed that the solid dispersion made with AAC and polyvinyl pyrrolidone (PVP) effectively changed the solubility of AAC and improved its bioavailability. The MN shape was conical and the bending forces of AAC/PVP-MN were all approximately 1.2 N/needle, which was enough to penetrate the stratum corneum of the skin. Through the use of the neuropathic pain model (spared nerve injury) test, it was found that the soluble MN mediated AAC hypodermic delivery provided effective analgesic activity. Compared with the model group, AAC/PVP-MN could increase mechanical pain threshold and hind legs load-bearing capacity, reduce the inflammation in the body, and have certain protective effect to spinal cord neurons. This study provided an idea for the clinical treatment of neuropathic pain and also a new approach for the safe use of toxic drugs with a narrow range.
研究领域:
药理学
·
疼痛管理
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 4.446
Shan Yuan,Miaomiao Jin,Chao Xu,Wencheng Yin,Mingsha Zhao,Mo Xian,Wei Liu
3-(Aminomethyl) pyridine (3-AMP) is a versatile compound for the synthesis of pharmaceuticals, agricultural chemicals and numerous polymers. Efficient and mild biocatalytic synthesis is often an excellent supplement to traditional chemical synthesis methods. However, there has been no report of biocatalytic 3-AMP synthesis. In this paper, 3-AMP was synthesized for the first time by using biocatalysts co-expressing carboxylic acid reductase from Neurospora crassa (ncCAR) and ω-transaminase from Ochrobactrum anthropi (OATA) in one pot. In addition, the metabolic pathway was optimized by knocking out the transcriptional dual regulator arcA , which resulted in a 90% increase in the yield of 3-AMP. Furthermore, efficient self-sufficient whole-cell bioconversion was established by optimization, achieving 93% yield and a 40.6 mM (4.4 g/L) titer of 3-AMP without additional carbon sources. This whole-cell bioconversion system was also suitable for the high-efficiency biocatalytic synthesis of other aromatic or heterocyclic primary amines, such as benzylamine, 2-furfurylamine, 4-fluorobenzylamine, 2,4-difluorobenzylamine and 2-(aminomethyl) pyridine. Being entirely biocatalytic, our one-pot procedure provides considerable advantages in terms of economic, environmental and safety impacts over reported chemical methods.
研究领域:
生物化学
·
合成生物学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Haoyue Ning,Lixin Lu,Jing Xu,Lijing Lu,Liao Pan,Zidong Lin
The purpose of this study was to compare the effects of different concentrations of the amine-functionalized isoreticular metal-organic framework-3 loaded with carvacrol (IRMOF-3/CA) on the properties of sodium alginate (SA) composite films, thus determining the optimal addition amount and further preparing bioactive packaging film with antibacterial and antioxidant activities. The morphology, structure, physical properties, antioxidant and antibacterial activities of the films were characterized and analyzed. The results showed that the thermal stability and light barrier property of the films were improved by the addition of IRMOF-3/CA. When the additional concentration was 0.4 wt%, the tensile, water vapor barrier and hydrophobic properties of the films were increased by 30.13%, 9.06% and 46.43% respectively compared with those of pure SA film. Moreover, the film added with IRMOF-3/CA had sustained antioxidant and antibacterial activities , and had an apparent fresh-keeping effect on pork, suggesting its application potential in food packaging.
研究领域:
食品科学
·
材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 16
·2472-2477
·
IF 2.7
Chenhao Zhao,Xingdan Wang,Jing Wu,Yeli Hu,Qiwei Zhang,Qi Zheng
Sialic acids are a family of monosaccharides that share a nine-carbon backbone and a carboxyl group. A recent derivatization method based on 3-nitrophenylhydrazine (3-NPH) provides a mild chemical labeling technique for biomolecules containing carbonyl or carboxyl groups. In this study, we utilized 3-NPH to label sialic acids via a two-step derivatization process. The derivatized species can produce a common reporter ion corresponding to C1–C3 with two labels, and a fragment differentiating between Neu5Ac, Neu5Gc, and KDN. This method is compatible with O-acetylated sialic acids and provides high sensitivity to Neu5Gc and KDN, and since the utilization of dual labeling significantly enhances the hydrophobicity of derivatives, it can effectively mitigate matrix effects when combined with parallel reaction monitoring technology. Negative-ion tandem mass spectrometry (MS/MS) analysis reveals a distinctive fragmentation profile for the 4-O-acetylated species, while the other sialic acids yield similar MS/MS spectra with a high abundance of reporter ions. Using the reporter ion as a transition, this analytical strategy is effective for analyzing complex biological samples. For example, it was successfully employed to quantify sialic acids in the intestinal tissues of several carp species, demonstrating its potential in sialylation research.
研究领域:
分析化学
·
糖生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Qin-Chao Wang,Zhaoquan Peng,Sha He,Hao Chen,Jinglong Du,Huilin Zang,Xiaoge Li,Xiuqin Zhan,Jie Han
Sodium superionic conductor NaTi 2 (PO 4 ) 3 has attracted significant interest as an anode material for sodium-ion batteries (SIBs). However, its practical application is hindered by its low inherent electrical conductivity, particularly at low temperatures. In this study, oxygen vacancies (V O ) were introduced into NaTi 2 (PO 4 ) 3 nanoribbons to enhance sodium storage performance at low temperatures. X-ray diffraction with Rietveld refinement, electron paramagnetic resonance, and X-ray photoelectron spectroscopy confirm that NaTi 2 (PO 4 ) 3 -2 nanoribbons (NTP-2) exhibit the richest V O concentration. These V O , which bridge TiO 6 octahedra and PO 4 tetrahedra, significantly enhance the antibonding interactions of Ti1–O2 and P1–O1 bonds, while stabilizing the bonding in NaTi 2 (PO 4 ) 3 . The energy barrier for Na + migration is reduced to 0.40 eV involving the V O . The optimized NTP-2 anode demonstrates superior low-temperature performance, maintaining a capacity of 106.1 mAh g −1 (about 96.1 % of its initial capacity) at −20 °C after 300 cycles. Additionally, the NTP-2 anode exhibits a moderate Na + diffusion coefficient of 1.47 × 10 –11 cm 2 s −1 at −20 °C. Furthermore, the Na 3 V 2 (PO 4 ) 3 //NTP-2 full cell retains a capacity of 64 mAh g −1 at −20 °C after 250 cycles, highlighting its potential for low-temperature applications. By integrating oxygen vacancies and nanoengineering, both electronic and ionic conductivities are significantly enhanced in NaTi 2 (PO 4 ) 3 , positioning promising applications for SIBs in low-temperature environments.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2018
·Vol. 6
·期 36
·9778-9785
·
IF 5.976
Qiang Zhang,Yan Xu,Zhuopeng Wang,Xia Zhang,Zhiguo Xia
Dual-emitting hierarchical Bi4(GeO4)3/Zn2GeO4 hybrid microspheres have been prepared by a facile one-pot hydrothermal synthesis of Zn2GeO4 with the addition of pre-synthesized BiOBr nanoplates. The phase composition and morphology evolution of the Bi4(GeO4)3/Zn2GeO4 composites are investigated depending on the amounts of BiOBr precursor used in the hydrothermal process. The photoluminescence peaks at 412 and 532 nm for the as-prepared Bi4(GeO4)3/Zn2GeO4 are ascribed to Bi3+ emission and defect-related centers in the Zn2GeO4 component, respectively, and the features show a quenching response toward a temperature increase. The fluorescence intensity ratio (FIR) of Bi3+: 3P1–1S0 (412 nm) to the defect emission center of Zn2GeO4 (532 nm) can be exploited as a self-calibrated temperature index for potential thermometer applications. The maximum relative sensitivity (Sr) is 3.96% °C−1 in the temperature range of 20 °C to 100 °C. This work expands the rational design strategy for controllable particle morphology and structure modulation, and further explores their synergetic distinctive properties and potential optical sensor applications.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）