RSC Advances
·
2016
·Vol. 6
·期 20
·16500-16506
·
IF 3.289
Min Su,Haiyun Liu,Shenguang Ge,Na Ren,Longhua Ding,Jinghua Yu,Xianrang Song
In this work, a novel paper-based electrochemiluminescence (ECL) immunosensor for ultrasensitive and selective detection of corresponding antigens on living cells was developed employing AuPd nanoparticles as a signal amplifier and macroporous Au-paper working electrodes (Au-PWE) for efficient cell capture. The porous AuPd nanoparticles are chosen as label nanomaterials due to their controllable three-dimensional porosity and peroxidase like activity, large surface area, superior conductivity, and favorable catalytic activity to H2O2 which could enhance the ECL intensity for signal amplification. CdTe QDs and luminol groups as ECL probes were linked with the antibodies to recognize the corresponding antigens on the cell surface and the wide potential range between them compared to conventional antigens sensors could prevent interfacing with each other. Using this strategy, human carcinoembryonic (CEA) and alphafetoprotein (AFP) antigens on living MCF-7 cancer cells as models were quantified serially through a potential scanning way. Significantly, this strategy may be quite promising in cancer early diagnosis due to the excellent analytical performance.
研究领域:
生物医学工程
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分析化学
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肿瘤学
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产品关联
DOI
高影响因子
RUSSIAN JOURNAL OF GENERAL CHEMISTRY
·
2022
·Vol. 92
·期 10
·2119-2131
·
IF 0.779
Wang Yanyan,Chen Ruicheng,Hu Yiping,Jiao Shulin,Zou Zhihong
Abstract In this study, a novel series of 7-azaindole-1,2,3-triazole bearing N -benzamide derivatives 3a – 3r were synthesized and fully characterized by 1 H NMR, 13 C NMR, and MS. The prepared compounds were tested against A549 and HepG2 cell lines by using MTT assay in vitro anticancer activity. Compounds 3d , 3l , 3q , and 3r exhibited the most promising, with IC 50 (7.83, 7.58, 9.47, and 8.65 μM, respectively) against A549 compared with Gefitinib (IC 50 , 6.77 μM) and IC 50 (6.98, 6.69, 10.18, and 7.97 μM, respectively) against HepG2 compared with Sorafenib (IC 50 , 5.65 μM). Further molecular docking studies showed that compounds 3d , 3l , 3q , and 3r can bind well to protein targets 3LXY, 4AGD, and 3WZE, so these compounds have the potential to be developed into multi-targeted kinase inhibitor.
研究领域:
药理学
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化学
·
肿瘤学
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DOI
高影响因子
FEBS LETTERS
·
2015
·
IF 3.169
Chen Xie,Wei Chen,Mengdie Zhang,Qiuxian Cai,Weiyi Xu,Xiaodi Li,Songshan Jiang
Despite extensive investigation into the role of let-7 miRNAs in pathological tumor processes, their involvement in the DNA damage response remains unclear. Here we show that most let-7 family members down-regulate MDM4 expression via binding to MDM4 mRNA at a conserved DNA sequence. Expression of exogenous let-7 miRNA mimics decreased MDM4 protein but not mRNA levels. Several DNA damage reagents increased let-7 expression, thereby decreasing MDM4 protein levels in glioma cells. Inhibition of endogenous let-7 with antisense RNAs rescued MDM4 protein levels with or without MG132, a proteasome-dependent degradation inhibitor. An MDM4 mutation identified in a glioma patient was associated with loss of the putative MDM4 target site. Therefore, let-7 binding to MDM4 is implicated in the DNA damage response.
研究领域:
癌症生物学
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分子生物学
·
遗传学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 15
·15218-15226
·
IF 2.76
Yue Xinxin,Guo Weilin,Li Xianghui,Zhou Haihong,Wang Ruiqin
In this study, a novel core-shell Fe 3 O 4 @MIL-101 (MIL stands for Materials of Institute Lavoisier) composite was successfully synthesized by hydrothermal method and was fully characterized by X-ray diffraction, transmission electron microscopy, Fourier-transform infrared spectra, and X-ray photoelectron spectroscopy. The composite was introduced as a catalyst to generate powerful radicals from persulfate for the removal of Acid Orange 7 in an aqueous solution. Effects of the central metal ions of MIL-101, amino group content of MIL-101, and pH were evaluated in batch experiments. It was found that both hydroxyl and sulfate radicals were generated; importantly, sulfate radicals were speculated to serve as the dominant active species in the catalytic oxidation of Acid Orange 7. In addition, a possible mechanism was proposed. This study provides new physical insights for the rational design of advanced metal-organic frameworks (MOF)-based catalysts for improved environmental remediation.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 183
·期 2
·683-688
·
IF 3.741
Jia Shengsong,Li Pengfei,Koh Kwangnak,Chen Hongxia
The article describes the use of a designed histidine (His)-tagged arginine-glycine-aspartic acid (RGD) peptide as a linker to bind integrin to captured cancer cells, and how to amplify the surface plasmon resonance (SPR) signal after binding of NiO nanoparticles (NiO-NPs) via the His-tag on the peptide. Specifically, breast cancer cells were captured via the interaction between human mucin (MUC-1) and a gold surface modified with a MUC-selective aptamer. The resulting cytosensor exhibits specificity and high sensitivity which is due to the enhancement of the SPR signal by NiO-NPs. The breast cancer cell line MCF-7 can be easily distinguished from normal islet beta cells by using this biosensor. Implementation of the His-tagged RGD peptide modified with NiO-NPs resulted in 20-fold enhancement of the SPR signal at the limit of detection. Hence, the actual limit of detection is lowered to 136 cells per mL. In our perception, this cytosensor has a large clinical potential in that it may also be used to detect various other kinds of tumor cells.
Histidine-tagged arginine-glycine-aspartic acid peptide carried by NiO interacts with the integrin of cancer cells and amplifies the SPR signal resulting from interaction between human mucin (MUC-1) and a gold surface modified with a MUC-selective aptamer. As little as 136 cells·mL −1 are detectable.
研究领域:
生物传感技术
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肿瘤检测
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医学诊断
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产品关联
DOI
高影响因子
ACS Applied Optical Materials
·
2023
·
IF 0
Long-Jing Wu,Rui Fu,Guozheng Huang,Rui-Lian Lin,Jing-Xin Liu
The design and construction of near-infrared (NIR) photochromic materials that show absorption and responsiveness in the NIR region remain a challenge. In this work, we prepared two triazine-bridged viologens, one being 2,4,6-tri(4-pyridinium)-1,3,5-triazine trichloride (TPA·Cl3) and the other one being its tribenzyl-substituted derivative (TPB·Br3). 1H NMR spectroscopy reveals that the cucurbit[7]uril (Q[7]) can bind with the two viologens to form quaternary inclusion complexes TPA3+@Q[7]3 and TPB3+@Q[7]3. Both inclusion complexes demonstrate NIR photochromic behavior, and the color changed from white to bluish green. Electron-spin resonance (ESR) spectra indicate that their photochromism is due to the formation of free radicals TPA•2+/TPB•2+. In addition, the inclusion complexes show photomodulable fluorescence emission. The excellent photochromic behavior and photomodulable fluorescence endow the inclusion complexes with practical utility in erasable inkless printing and anticounterfeiting.
研究领域:
化学
·
材料科学
·
物理化学
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DOI
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-10
·
IF 5.479
Dong Nan,Zhang Lingxue,Yao Jianmei,Ma Peijian,He Jing,Li Tao,Wang Yuan
A detailed study has been carried out on monohydroxycucurbit[7]uril-based stir-bar sorptive extraction (SBSE). A polydimethylsiloxane coating was produced by a sol–gel technique and doped with monohydroxycucurbit[7]uril ((HO) 1 Q [7]) as a selective sorbent phase. (HO) 1 Q [7] was chemically bound to the sol–gel silica substrate through hydrolysis and polycondensation. The coating possesses a porous surface, shows strong solvent resistance and good thermal stability, and has a long lifespan. Four groups of compounds, with polarities ranging from apolar polycyclic aromatic hydrocarbons to polar ketones, aromatic amines and phenols, were selected as test analytes. They were extracted with the coated stir bar, then desorbed with methanol and quantified by high-performance liquid chromatography with ultraviolet detection. The limits of detection range between 1.3 and 15 μg L −1 , the linear ranges extend from 5 to 10,000 μg L −1 , and the relative recoveries from spiked samples range between 76.4 and 97.9%. The intraday relative standard deviations range from 2.3 to 8.6% (for n = 3, at 500 μg L −1 ). Compared with a commercial PDMS-coated stir bar and a polyether sulfone-coated stir bar, the new stir bar shows wider applicability and better extraction efficiency for each group of compounds. In addition, the stir bar can simultaneously extract mixtures of chemicals of different polarities. This endows it with the potential for recovering a broad group of polar organic compounds.
A stir bar for sorptive extraction based on monohydroxycucurbit[7]uril has been prepared by a sol–gel technique. The stir bar coupled with HPLC with UV detection allows for the determination of a variety of polar compounds and multicomponent mixtures, respectively.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ZOOLOGICAL RESEARCH
·
2024
·Vol. 45
·期 6
·1287
·
IF 4
Yu-Tong Wu,Ze-Qiong Ru,Ying Peng,Zhe Fu,Qiu-Ye Jia,Zi-Jian Kang,Yuan-Sheng Li,Yu-Bing Huang,Sai-Ge Yin,Kun Guo,Nai-Xin Liu,Cheng-An Feng,Jing Tang,Bai-Yu Zhang,Zhi Yang,Ying Wang,Xin-Wang Yang
Individuals with diabetes frequently face serious challenges, including delayed wound healing and increased risk of infection. Notably, the regeneration of hair follicles plays a crucial role in accelerating diabetic skin damage repair, reducing the risk of infection, and enhancing overall skin health. Research has predominantly emphasized the re-epithelialization of diabetic wounds, with a paucity of in-depth studies on hair follicle regeneration. In the current study, we explored the effects of a bioactive amphibian-derived peptide, Cy RL-QN15 , on promoting hair regeneration in a diabetic skin model. In vivo experiments demonstrated that local treatment with Cy RL-QN15 not only accelerated wound healing of scalded skin on the backs of diabetic Kunming (KM) mice but also improved growth of damaged hair follicles. Additionally, back-shaved diabetic C57BL/6 mice showed a significant increase in the growth of newly formed hair after 28 days of continuous Cy RL-QN15 treatment. Further analysis indicated that the hair-regenerating effects of Cy RL-QN15 were closely associated with the proliferation and migration of hair follicle stem cells (HFSCs). Cy RL-QN15 enhanced intracellular β-catenin expression by binding to the Frizzled-7 receptor on the surface of HFSCs. The up-regulation in β-catenin modulated the levels of downstream proteins, such as c-MYC, Cyclin D1, and Lef1, ultimately inducing hair regeneration. This study not only reveals the robust effects of the bioactive peptide Cy RL-QN15 in hair follicle regeneration but also provides novel avenues for the development of more targeted and effective therapeutics for diabetic wound healing in the future.
研究领域:
糖尿病研究
·
伤口愈合
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干细胞生物学
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肽疗法
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分子生物学
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DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Xilin Huang,Xuli Gao,Junbao Huang,Yani Luo,Wei Tao,Mingyu Guo,Yongyuan Liu,Jing Wu,Yu Chen,Yan Liu
A recombinant strain (BS168-Δ bdhA ) that increased co-production of MK-7 and NK was constructed by knocking out the 2,3-butanediol dehydrogenase gene ( bdhA ) of Bacillus subtilis 168 to reduce the carbon spillover. The results showed that the 2,3- butanediol content in the recombinant strain was reduced by 64% to 2.76 g/L. The recombinant strain had an MK-7 production of 30.6 mg/L and an NK activity of 6.5 FU/mL. RNA-Seq analysis showed that: bdhA knockdown blocked the carbon flux of 2,3-butanediol and promoted glycerol uptake, and the carbon flux flowed more to the MK-7 and NK synthesis pathways. The negative regulator of NK, codY , was down-regulated by 2.19-fold. secA , which is responsible for the energy supply of transmembrane proteins, was down-regulated by 0.37-fold, the Tat secretion pathway was up-regulated by 2.8-fold and 0.5-fold for tatAD and tatC , respectively. This study revealed the mechanism of co-production of MK-7 and NK in recombinant strains for the first time and revealed potential molecular targets to promote the co-production of MK-7 and NK, which will provide a new strategy for metabolic engineering to increase the production of MK-7 and NK.
研究领域:
微生物学
·
分子生物学
·
代谢工程
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产品关联
DOI
高影响因子
ACS Omega
·
2018
·
IF 0
Xiao Tian,Li Xia Chen,Yu Qing Yao,Kai Chen,Min-Dong Chen,Xi Zeng,Zhu Tao
Upon mixing of aqueous solutions of the freely soluble building blocks cucurbit[7]uril (Q[7]) and 4-sulfocalix[4]arene (SC[4]A), white microcrystals instantly separate in near-quantitative yield. The driving force for this assembly is suggested to be the outer-surface interaction of the Q[n]. Dynamic light scattering, scanning electron microscopy, and NMR (diffusion-ordered NMR spectroscopy) analyses have confirmed the supramolecular aggregation of Q[7] and SC[4]A. Titration 1H NMR spectroscopy and isothermal titration calorimetry have shown that the interaction ratio of Q[7] and SC[4]A is close to 3:1. Moreover, the Q[7]/SC[4]A-based supramolecular assembly can accommodate molecules of some volatile compounds or luminescent dyes. Thus, this work offers a simple and highly efficient means of preparing adsorbent or solid fluorescent materials.
研究领域:
化学
·
超分子化学
·
材料科学
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DOI
PubMed
PHYTOTHERAPY RESEARCH
·
2024
·
IF 6.1
Xinyi Qi,Yurou Zhang,Qichao Liao,Yang Xiao,Tianyu Jiang,Siqi Liu,Lei Zhou,Yixing Li
The escalating incidence of nonalcoholic fatty liver disease (NAFLD) is closely associated with a high-fat diet, leading to a decline in quality of life and significant health impairment. 7-Hydroxyflavone (7-HY) is a flavonoid known for its anti-inflammatory, anticarcinogenic, and antioxidant effects. This study aims to assess the ameliorative effects of 7-HY on NAFLD induced by a high-fat diet and elucidate underlying mechanisms. Oleic acid/palmitic acid-induced HepG2 cells and C57BL/6 mice on a high-fat diet were utilized as in vitro and in vivo models. In animal experiments, 7-HY was utilized as a dietary supplement. The 15-week in vivo experiment monitored body weight, body fat percentage, glucose tolerance, insulin tolerance, and metabolic indexes. Commercial kits assessed triglyceride (TG) and total cholesterol levels in cells, liver tissue, and blood. Discovery Studio identified potential targets of 7-HY, compared with NAFLD-associated targets in the GeneCards database. Results indicated 7-HY mitigated fat accumulation, hepatic steatosis, and oxidative stress induced by a high-fat diet. Furthermore, 7-HY showed potential efficacy in ameliorating abnormal glucose metabolism and promoting energy metabolism. Reverse target finding and molecular docking demonstrated a robust interaction between 7-HY and serine/threonine kinase 24 (STK24). Subsequent experimental results confirmed 7-HY's ability to inhibit TG deposition in HepG2 cells through interaction with STK24. In conclusion, 7-HY demonstrated the capacity to alleviate high-fat diet-induced NAFLD, presenting a novel strategy for the prevention and treatment of NAFLD.
研究领域:
药理学
·
分子生物学
·
代谢疾病
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Peigang Zhang,Yunhui Fu,Tianyi Zheng,Huihui Ge,Martin M.F. Choi,Li Zhang,Wei Bian
Glutathione (GSH), an essential biothiol, plays a critical role in reversible redox reactions, cellular signaling, detoxification, and metabolism. Fluctuations in GSH levels are closely linked to serious health conditions, underscoring the significance of monitoring its concentration. Fluorescence-based sensing with nanomaterials offers a promising strategy for rapid GSH detection. In this work, we developed a fluorescent probe integrating 7-amino-4-methylcoumarin (AMC) and cobalt oxyhydroxide nanoflakes (CoOOH NFs) for GSH detection through a combined internal-filter effect and static quenching mechanism. The introduction of GSH triggered a redox reaction that degraded CoOOH NFs, suppressing their quenching effect and restoring AMC fluorescence, which enabled GSH quantification through fluorescence recovery. The probe detected GSH in the range of 20–200 μM with a detection limit of 0.13 μM. It was successfully applied to determine GSH in human serum, and the results showed excellent agreement with those measured by a commercial GSH detection kit based on the use of Ellman's reagent.
研究领域:
分析化学
·
纳米技术
·
生物化学
·
传感器
·
荧光光谱学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）