Horticulture Research
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2024
·
IF 7.6
Yuan Ziyu,Li Gu,Zhang Huixian,Peng Zhaoxin,Ding Wenyu,Wen Huan,Zhou Hanxin,Zeng Jiwu,Chen Jiajing,Xu Juan
Citrus fruits have abundant flavonoid glycosides (FGs), an important class of natural functional and flavor components. However, there have been few reports about the modification of UDP-glycosyltransferases (UGTs) on flavonoids in citrus. Notably, in flavonoid biosynthesis, 7-O-glucosylation is the initial and essential step of glycosylation prior to the synthesis of flavanone disaccharides, the most abundant and iconic FGs in citrus fruits. Here, based on the accumulation of FGs observed at the very early fruit development stage of two pummelo varieties, we screened six novel flavonoid 7-O-glucosyltransferase genes (7GlcTs) via transcriptomic analysis and then characterized them in vitro. The results revealed that four Cg7GlcTs possess wide catalytic activities towards various flavonoid substrates, with CgUGT89AK1 exhibiting the highest catalytic efficiency. Transient overexpression of CgUGT90A31 and CgUGT89AK1 led to increases in FG synthesis in pummelo leaves. Interestingly, these two genes had conserved sequences and consistent functions across different germplasms. Moreover, CitUGT89AK1 was found to play a role in the response of citrus to Huanglongbing infection by promoting FG production. The findings improve our understanding of flavonoid 7-O-glucosylation by identifying the key genes, and may help improve the benefits of flavonoid biosynthesis for plants and humans in the future.
研究领域:
植物科学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
Journal of Functional Foods
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2015
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IF 3.574
Fengzhong Wang,Huifang Wang,Donghui Wang,Fang Fang,Jixiang Lai,Tao Wu,Rong Tsao
The changes in isoflavone and γ-aminobutyric (GABA) contents and antioxidant activity of germinated soybean seeds were investigated. Three Chinese soybean cultivars (ZH 13, 30 and 42) released for high yield and protein content were evaluated. The results showed that the total isoflavone contents at peak level were 86.3, 81.0, and 42.3% higher than that of the un-germinated control for ZH 42, 13 and 30, respectively. GABA content of ZH 13 in particular was a statistically significant 36.7-fold increase in day 5 germinated soybeans as compared to un-germinated soybean. Such increases also led to significantly elevated antioxidant activities . Moreover, germination can significantly suppress IL-8 expression in Caco-2 cells by ZH 13 extract ( P < 0.05) without cell apoptosis. Current results suggest that ZH 13 soybean is a cultivar that can be used to develop nutritional and functional foods with important health benefits.
研究领域:
食品科学
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植物学
·
营养学
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DOI
高影响因子
DEVELOPMENT
·
2024
·
IF 3.7
Yu Meng,Thorner Konrad,Parameswaran Sreeja,Wei Wei,Yu Chuyue,Lin Xinhua,Kopan Raphael,Hass Matthew R.
The conserved Runt-related (RUNX) transcription factor family are master regulators of developmental and regenerative processes. Runx1 and Runx2 are expressed in satellite cells (SC) and in skeletal myotubes. Conditional deletion of Runx1 in adult SC negatively impacted self-renewal and impaired skeletal muscle maintenance even though Runx2 expression persisted. Runx1 deletion in C2C12 cells that retain Runx2 expression identified unique molecular functions of Runx1 that cannot be compensated by Runx2. The reduced myoblast fusion in vitro caused by Runx1 loss was due in part to ectopic expression of Mef2c, a target repressed by Runx1 . Structure-function analysis demonstrated that the Ets-interacting MID/EID region of Runx1, absent from Runx2, is critical to Runx1 myoblast function and for Etv4 binding. Analysis of ChIP-seq datasets from Runx1 (T-cells, muscle) versus Runx2 (preosteoblasts) dependent tissues identified a composite Ets:Runx motif enriched in Runx1 -dependent tissues. The Ets:Runx composite motif was enriched in peaks open exclusively in ATAC-seq datasets from WT cells compared to ATAC peaks unique to Runx1KO cells. Thus, engagement of a set of targets by the RUNX1/ETS complex define the non-redundant functions of Runx1 .
研究领域:
分子生物学
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细胞生物学
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转录因子
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肌肉再生
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 21
·13506
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IF 6.208
Guobin Li,Lan Zhang,Zhe Lu,Baoqiang Yang,Hui Yang,Peng Shang,Jean X. Jiang,Dong’en Wang,Huiyun Xu
Osteoporosis and sarcopenia (termed “Osteosarcopenia”), the twin-aging diseases, are major contributors to reduced bone mass and muscle weakness in the elderly population. Connexin 43 (Cx43) in osteocytes has been previously reported to play vital roles in bone homeostasis and muscle function in mature mice. The Cx43-formed gap junctions (GJs) and hemichannels (HCs) in osteocytes are important portals for the exchange of small molecules in cell-to-cell and cell-to-extracellular matrix, respectively. However, the roles of Cx43-based GJs and HCs in both bone and muscle aging are still unclear. Here, we used two transgenic mouse models with overexpression of the dominant negative Cx43 mutants primarily in osteocytes driven by the 10-kb Dmp1 promoter, R76W mice (inhibited gap junctions but enhanced hemichannels) and Δ130–136 mice (both gap junction and hemichannels are inhibited), to determine the actions of Cx43-based hemichannels (HCs) and gap junctions (GJs) in the regulation of bone and skeletal muscle from aged mice (18 months) as compared with those from adult mice (10 months). We demonstrated that enhancement of Cx43 HCs reduces bone mass due to increased osteoclast surfaces while the impairment of Cx43 HCs increases osteocyte apoptosis in aged mice caused by reduced PGE2levels. Furthermore, altered mitochondrial homeostasis with reduced expression of Sirt-1, OPA-1, and Drp-1 resulted in excessive ROS level in muscle soleus (SL) of aged transgenic mice. In vitro, the impairment of Cx43 HCs in osteocytes from aged mice also promoted muscle collagen synthesis through activation of TGFβ/smad2/3 signaling because of reduced PGE2levels in the PO CM. These findings indicate that the enhancement of Cx43 HCs while GJs are inhibited reduces bone mass, and the impairment of Cx43 HCs inhibits PGE2level in osteocytes and this reduction promotes muscle collagen synthesis in skeletal muscle through activation of TGFβ/smad2/3 signaling, which together with increased ROS level contributes to reduced muscle force in aged mice.Keywords:osteocytes;hemichannels;gap junctions;bone-muscle crosstalk;aging
研究领域:
生物学
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生理学
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老年学
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肌肉骨骼系统
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DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2023
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IF 6.5
Yuedong He,Jing Zhang,Yuewei He,Huhu Liu,Chong Wang,Guiping Guan,Yucheng Zhao,Yun Tian,Xiaohong Zhong,Xiangyang Lu
Angelica decursiva , an important traditional medicinal plant, possesses a unique pharmacological activity. Its principal active ingredients are coumarins, including scopoletin, bergapten, and imperatorin. However, the enzymes catalyzing the critical step of coumarins biosynthesis pathway remain unidentified. This study initially screened 14 candidate O -methyltransferases (OMTs) through transcriptomics and metabolic determination. Combined with gene expression profile and biochemical assays, two OMTs (AdOMT1 and AdOMT2) were identified to be responsible for the O -methylation of coumarins in A. decursiva. AdOMT1 showed higher catalytic efficiency for bergaptol (Kcat/Km = 3123.70), while AdOMT2 exhibited higher substrate and catalytic promiscuity, allowing it to catalyze the methylation of various coumarins, phenylpropanes, and flavonoids. Based on molecular docking and site-specific mutagenesis determined that His126/Asn132, Phe171/Phe177, Trp261/Trp267, and Asn312/Ile317 were the key catalytic residues of AdOMT1 and AdOMT2 for the O -methylation of bergaptol and xanthotoxol. Further phylogenetic analysis confirmed the reasons for the catalytic functional differentiation of AdOMT1 and AdOMT2. This study provides a basis for exploring the coumarins O -methylation mechanism and plays a critical role in diversifying the structures used in coumarins drug discovery.
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高影响因子
Foods
·
2023
·Vol. 12
·期 10
·2011
·
IF 5.2
Lilai Mo,Xinzhuan Yao,Hu Tang,Yan Li,Yujie Jiao,Yumei He,Yihe Jiang,Shiyu Tian,Litang Lu
Cuticular wax is a complex mixture of very long-chain fatty acids (VLCFAs) and their derivatives that constitute a natural barrier against biotic and abiotic stresses on the aerial surface of terrestrial plants. In tea plants, leaf cuticular wax also contributes to the unique flavor and quality of tea products. However, the mechanism of wax formation in tea cuticles is still unclear. The cuticular wax content of 108 germplasms (Niaowang species) was investigated in this study. The transcriptome analysis of germplasms with high, medium, and low cuticular wax content revealed that the expression levels ofCsKCS3andCsKCS18were strongly associated with the high content of cuticular wax in leaves. Hence, silencingCsKCS3andCsKCS18using virus-induced gene silencing (VIGS) inhibited the synthesis of cuticular wax and caffeine in tea leaves, indicating that expression of these genes is necessary for the synthesis of cuticular wax in tea leaves. The findings contribute to a better understanding of the molecular mechanism of cuticular wax formation in tea leaves. The study also revealed new candidate target genes for further improving tea quality and flavor and cultivating high-stress-resistant tea germplasms.Keywords:cuticle wax;KCSgene family;tea plant (Camellia sinensis);wax biosynthesis
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PubMed
高影响因子
Translational Research
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2022
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IF 10.171
Tiantian Wang,Han Gao,Chengwei He,Lei Gao,Boya Wang,Rongxuan Hua,Yixuan Du,Chen Liang,Shuzi Xin,Hongwei Shang,Ying Wang,Wen Wang,Jingdong Xu
ABSTRACT Hypertension (HTN) is a common endpoint for numerous cardiovascular diseases, the prevalence of which has been quickly increasing due to a wide range of reasons. Previous research has found that following stress, ELISA and 16S rDNA sequencing indicated substantial changes in plasma cytokines or hormones, as well as alterations in gut microbiota in juvenile hypertensive rats. However, it remains still unclear how such interaction modifications affect microbial populations and organismal function. Stress-related hormones show a significant drop. Similar to earlier research, the stress group had dramatically increased release of pro-inflammatory cytokines such as IL-17. Importantly, a unified collection of tools that allows for deep and comprehensive colonic structural investigation has been developed. Stress may limit the transition of macrophages (Mφs) to M1Mφs while increasing the transfer to M2Mφs. Evidence highlighted that tight junction proteins were decreased along with enhancement in intestinal permeability . Morphological analysis revealed that the SHR-S group exhibited considerably higher levels of morphological alterations and fibrosis in colon, heart, and thoracic aorta tissues.Significant improvements in bacteria linked with short-chain fatty acid synthesis, such as Prevotella and Ruminococcus , were discovered by metagenomic analysis. Adult hypertensive rats are more susceptible to gut microbiota disruption and fibrosis as a result of mild restraint stress . This might contribute to some innovative ideas for HTN both treatment and prevention.
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PubMed
高影响因子
Journal of Physical Chemistry C
·
2023
·
IF 3.7
Chunyu Yu,Xue Li,Xin Qiao,Zhiwei Zhang,Danyang Zhang,Hua Liu,Xue-Li Hao,Zhongzhi Wang,Hongpeng You,Liang Zhou
Fluorescent carbon quantum dots (r-CDs) were synthesized safely and readily by stirring at room temperature with a yield of 76.5%. The synthesized r-CDs exhibit bright green emission with an absolute quantum yield of 12.0% under the excitation of ultraviolet light. Within the pH range of 3–12, the r-CDs exhibit excellent fluorescence stability. Interestingly, the fluorescence intensity of r-CDs faded promptly when the pH value diminished to 2 and then regained when the pH value returned to 3. This means that r-CDs can be used as “on–off–on” fluorescent probes for pH sensing. The mechanism of this sensitive pH response behavior was discussed in detail, and a possible mechanism of protonation–deprotonation–protonation processes was proposed. In addition, we have proposed a green preparation route for eco-friendly filter paper using anticounterfeiting. Interestingly, the presence of Fe2+ significantly quenched the fluorescence of r-CDs. Experimental results demonstrated the r-CDs can also be used to detect the content of Fe2+ in water, and the detection limit was 8.82 μM/L.
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2022
·
IF 6.371
Yinchen Dai,Wubing Chen,Baochun Guo,Xiao Li,Jibiao Guan,Lina Wang,Ming Zhang
Efficient and inexpensive electrocatalysts play an important role in electrolysis of water and hydrogen evolution reaction . The catalytic activity of electrocatalyst can be improved by adjusting the electronic structure and increasing the active center. In this study, Ni 2 P nanosheets were grown on carbon cloth through straightforward solvothermal, and tiny MoS 2 nanosheets were uniformly covered on Ni 2 P nanosheets by in-situ growth method to form heterogeneous electrocatalyst (Ni 2 P @ MoS 2 /CC). The results show that Ni 2 P @ MoS 2 /CC had more active sites than Ni 2 P/CC and MoS 2 /CC catalysts. In addition, the interface interaction based on heterogeneous structure promotes its charge transfer kinetics. In alkaline electrolyte , Ni 2 P @ MoS 2 /CC electrocatalyst had good HER performance. At a current density of 10 mA cm −2 , the overpotential was 99 mV, and the Tafel slope was 97 mV dec −1 . In addition, the catalyst showed excellent electrochemical stability, with no significant loss of activity after 2000 cyclic voltammetry tests and 50 h i-t tests.
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高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2021
·
IF 9.028
Yun Wu,Wenzhe Wei,Jiayi Luo,Yang Pan,Mengting Yang,Ming Hua,Wenhai Chu,Chendong Shuang,Aimin Li
In recent years, dozens of halogenated disinfection byproducts (DBPs) with cyclic structures were identified and detected in drinking water globally. Previous in vivo toxicity studies have shown that a few new cyclic DBPs possessed higher developmental toxicity and growth inhibition rate than common aliphatic DBPs; however, in vitro toxicity studies have proved that the latter exhibited higher cytotoxicity and genotoxicity than the former. Thus, to provide a more comprehensive toxicity comparison of DBPs from different endpoints, 11 groups of cyclic DBPs and nine groups of aliphatic DBPs were evaluated for their comparative in vitro and in vivo toxicity using human hepatoma cells (Hep G2) and zebrafish embryos. Notably, results showed that the in vitro Hep G2 cytotoxicity index of the aliphatic DBPs was nearly eight times higher than that of the cyclic DBPs, whereas the in vivo zebrafish embryo developmental/acute toxicity indexes of the cyclic DBPs were roughly 48–50 times higher than those of the aliphatic DBPs, indicating that the toxicity rank order differed when different endpoints were applied. For a broader comparison, a Pearson correlation analysis of DBP toxicity data from nine different endpoints was conducted. It was found that the observed Hep G2 cytotoxicity and zebrafish embryo developmental/acute toxicity in this study were highly correlated with the previously reported in vitro CHO cytotoxicity and in vivo toxicity in aquatic organisms (P < 0.01), respectively. However, the observed in vitro toxicity had no correlation with the in vivo toxicity (P > 0.05), suggesting that the toxicity rank orders obtained from in vitro and in vivo bioassays had large discrepancies. According to the observed toxicity data in this study and the candidate descriptors, two quantitative structure–activity relationship (QSAR) models were established, which help to further interpret the toxicity mechanisms of DBPs from different endpoints.
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Huan Tan,Ruiyun Zhang,Liyang Han,Tong Zhang,To Ngai
Herein, gelatin was modified with ethylenediamine and processed into nanoparticles , named aminated gelatin nanoparticles (AGNPs). They had higher surface charge and were more hydrophobic compared with native gelatin nanoparticles (GNPs). The freely extending outer polymer layer of AGNPs is easy to be protonated or deprotonated at various pH conditions, giving AGNPs a remarkable size change of 63.23 nm and highly deformable property. AGNPs were then used as stabilizers to fabricate stable oil-in-water (O/W) Pickering emulsions using different edible oils (MCT oil and corn oil) as internal phase with volume fraction up to 85%. Most of AGNPs tended to irreversibly adsorb at oil−water interface to form independent droplets, whereas GNPs favored accumulating in aqueous phase, trapping oil droplets to form three-dimensional networks. The droplet size of AGNPs-stabilized emulsions could outstrip 150 μm and the emulsions still exerted stability against 10 months, centrifugation up to 10000 g, and pH changes. Increasing oil fraction led to form compact packing of oil droplets and in turn improve creaming stability, centrifugation stability, rheology, oxidation stability, as well as retention of encapsulated β-carotene of the emulsions. AGNPs-stabilized emulsions were generally more stable than GNPs-stabilized emulsions and could provide stronger protective features for lipid oxidation or entrapped β-carotene, especially for the cases in corn oil emulsions. Owing to the viscoelastic absorbed AGNPs layers, the surface-active long-chain fatty acids in corn oil and the incorporated β-carotene displayed very limited effect on the stability and microstructure of the emulsions. The findings in this work provided a perspective about ultra-stable Pickering emulsions with independent droplets merely stabilized by protein nanoparticle adsorbed layers, which had potential in encapsulating and protecting easily-oxidized functional ingredients in food and pharmaceutical applications.
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高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jing Chen,Shuying Li,Ying Chen,Jiao Yang,Jianbin Dong,Xiaoquan Lu
5-Hydroxytryptamine (5-HT) is an important neurotransmitter, and its content in the human body is of great significance to human health. In this study, an l-cysteine-terminated triangular silver nanoplate loaded on a MXene (two-dimensional transition metal carbide or nitride) (Tri-AgNP/l-Cys/MXene) electrochemical sensor was used to detect 5-HT. As an electrically active amino acid with a sulfhydryl group, l-cysteine (l-Cys) forms a more stable Ag–S bond with silver nanoparticles, which can selectively substitute trisodium citrate (TSC) in TSC-capped triangular silver nanoplates (Tri-Ag-NP/TSC). Due to the good conductivity, biocompatibility, and large surface area, MXenes provide a good platform for loading Tri-AgNP/l-Cys. Under optimized conditions, the concentration range for detecting 5-HT with the sensor is 0.5–150 μM, and the limit of detection (LOD) is 0.08 μM (S/N = 3). For detecting 5-HT in actual serum samples, the sensor also showed a good recovery rate (95.38–102.3%), and the relative standard deviation was 2.2–3.4%.
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高影响因子
危险品化学品经营许可证（带存储）