SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Yao Chen,Ying Tang,Haifei Wang
In this contribution, fluorescence spectroscopy based on G-quadruplex formation was developed for the quantification of ATP in cell. In the absence of ATP, thioflavin T (ThT) dye can combine with the G-rich ATP aptamer to form an ATP aptamer-ThT G-quandruplex complex, resulting in the enhancement of fluorescence intensity; otherwise, fluorescence intensity of the system will weaken with the addition of ATP, because ATP has a strong affinity with G-rich ATP aptamer and can replace ThT to form an ATP aptamer-ATP complex. A calibration model based on generalized ratio quantitative analysis model was employed to mitigate the influence of scatterers and background absorbers in cell suspensions. The proposed fluorescence method was applied to the quantitative analysis of ATP in two type of cell lines, and achieved satisfactory quantitative results with accuracy comparable to that of the reference method–ATP detection kit. The limit of detection and limit of quantification of the proposed method for ATP in cell were estimated to be 0.22 and 0.66 μM, respectively. This proposed fluorescence method is highly simple and rapid, and does not require the use of fluorescent labeling.
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高影响因子
LUMINESCENCE
·
2021
·Vol. 36
·期 7
·1584-1591
·
IF 2.464
Yaning Qin,Zhaoyue Yan,Rui Liu,Chaojie Qu,Haichen Mao,Lingbo Qu,Ran Yang
In this work, novel types of nitrogen-doped carbon dots (N-CDs) were prepared from citric acid and glycine (GLY) as precursors through a simple pyrolysis method. The GLY-CDs showed strong fluorescence with a fluorescence quantum yield as high as 33.34% and good water solubility. The fluorescence of GLY-CDs could be selectively quenched by iron(III) ion (Fe 3+ ) resulting in the non-fluorescent complex. Due to the high affinity of Fe 3+ to adenosine-5′-triphosphate (ATP), the fluorescence of the GLY-CDs in GLY-CDs-Fe 3+ could be recovered by ATP. Thereby, quantitatively fluorescent turn-on detection of ATP could be achieved. The fluorescence recovery ratio was linearly proportional to the concentration of ATP with a detection limit as low as 15.0 nM, indicating the CDs have high sensitivity. The GLY-CDs were successfully employed in the detection of ATP in serum and cell lysates.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-9
·
IF 3.998
Zhang Lei,Weng Yi-jin,Liu Xiao,Gu Wen,Zhang Xia,Han Lei
Surface Enhanced Raman Scattering (SERS) has been widely applied in many research fields such as biological detection and chemical analysis. However, for the common Au nanoparticles, it’s too hard to guarante the three aspects: the great enhanced effect, the controllable aggregation and the uniformity of nanoparticles, the environmental friendliness and biocompatibility of nanoparticles. In this paper, phytate acid (IP6)-coated Au nanoparticles (IP6@Au NPs) are more stable and have a higher enhancement factor than Au nanoparticles. In order to achieve the uniformity of the spherical IP6-coated@Au nanoparticles (IP6@Au NPs), IP6 was used as a soft template. In the presence of IP6, IP6@Ag nanoparticles were first synthesized by reducing AgNO 3 with trisodium citrate, then IP6@Au NPs were obtained by reducing HAuCl 4 with Ag nanoparticles. The IP6@Au NPs exhibit excellent Raman signal enhancement by using p-aminothiophenol (4-ATP) as the probe molecules. The effects of Fe 3+ on the performance of IP6@Au NPs SERS substrates were also studied. The results show that SERS has the best enhancement effect when adding proper amount of Fe 3+ (0.56 PPM), and the limit of detection was 10 −7 M 4-ATP.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2020
·
IF 3.306
Qian Qiu,Ru-Ru Gao,Aming Xie,Yingzhi Jiao,Wei Dong
Content of adenosine triphosphate (ATP) is an essential index in the development of various diseases and even some malignant tumors. Biosensing for ATP with high sensitivity and accuracy is of fundamental importance in both biological research and medical diagnostics. Here we report a ratiometric fluorescent sensor for ATP based on multi-color silver nanoclusters (Ag NCs). This nanosystem contains two core components: a hairpin DNA integrated with an ATP aptamer, an anchor sequence and a special template segment for synthesis of green-emitting Ag NCs; a single-stranded DNA (ssDNA) which is incompletely complementary with the anchor sequence to be a duplex scaffold for red-emitting Ag NCs. Once exposed to the target, formation of the ATP/aptamer complexes opens the hairpin structure and releases the anchor sequence to hybridize with the ssDNA, accompanied by the “turn-off” of the green fluorescence and “turn-on” of the red fluorescence. By measuring the fluorescence intensity ratio of the two signals, we successfully establish a ratiometric sensor which exhibits sensitive and accurate assay performance toward ATP, with the limit of detection (LOD) as low as 0.38 μM. Meanwhile, this ratiometric sensor shows stable recovery in real sample assay, indicating their potential promise in practical applications.
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Applied Materials Today
·
2020
·
IF 8.352
Xiaoqin Zhu,Yan He,Zhongrui Liu,Ziting Zhu,Yuehua He,Jiewen Qiu,Danqiong Liu,Mingshu Mo,Peng Wang,Xiumei Tian,Pingyi Xu
Epilepsy is characterized by repeated spontaneous seizures and remains untreatable due to its complicate pathogenesis. Low-dose hydrogen sulfide (H 2 S) has been shown to exert antiepileptic and protective effects in the central nervous system, but the administration of H 2 S gas to suppress seizures is difficult. In the present study, we synthesized a safe and efficient carbazole-based H 2 S donor from aldehydes with a one-pot procedure and characterized this specific H 2 S donor via proton nuclear magnetic resonance ( 1 HNMR), scanning electron microscopy (SEM), and absorption spectroscopy. In vitro and in vivo risk assessments demonstrated that the H 2 S donor (up to a 400-μM concentration) had sufficient biocompatibility and membrane permeability and suppressed epileptic seizures. Importantly, the suppression of seizures was determined in the brain when the H 2 S donor was injected into the lateral ventricle in a rat model of advanced seizures. Furthermore, the mechanism by which the H 2 S donor suppressed the expression of seizures may have been related to H 2 S donor-induced increases in the expressions of the ATP-sensitive potassium channel (K ATP ) subunits, Kir6.2 and SUR1. Taken together, these assays suggested that our novel H 2 S donor may represent a potential therapeutic strategy for ameliorating seizures in epilepsy.
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CHEMOSPHERE
·
2023
·
IF 8.8
Jian Sun,Huayi Qu,Waseem Ali,Yan Chen,Tao Wang,Yonggang Ma,Yan Yuan,Jianhong Gu,Jianchun Bian,Zongping Liu,Hui Zou
Microplastics (MPs) and cadmium (Cd) are important environmental pollutants, that damage the liver. However, the effect and mechanism of combined Cd and MPs exposure on liver fibrosis are still largely unknown. In this study investigated, Cd + MPs exposure increased superoxide anion production and promoted extracellular ATP release compared with exposure to Cd or MPs individually. Cd + MPs increased inflammatory cell infiltration , activated the P2X7-NLRP3 signaling pathway, and promoted inflammatory factor release. Cd + MPs aggravated Cd- or MPs-induced liver fibrosis and induced liver inflammation. In AML12/HSC-T6 cell in vitro poisoning model, exposure of AML12 cells to Cd + MPs increased the opening of connexin hemichannels and promoted extracellular ATP release. Treatment of HSC-T6 cells with the supernatant of AML12 cells exposed to Cd + MPs significantly promoted HSC-T6 cell activation. Treatment of HSC-T6 cells with different concentrations of ATP produced similar results. TAT-Gap19TFA, an inhibitor of connexin hemichannels, significantly inhibited the ATP release and activation of Cd + MPs-treated HSC-T6 cells. Finally, the expression of the ATP receptor P2X7 was silenced in HSC-T6 cells, which significantly inhibited their activation. In conclusion, exposure to Cd + MPs promoted liver fibrosis through the ATP-P2X7 pathway and synergistically affected liver inflammation and fibrosis.
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Acta Pharmaceutica Sinica B
·
2022
·
IF 14.903
Jiulong Zhang,Xiaoyan Sun,Xiufeng Zhao,Chunrong Yang,Menghao Shi,Benzhuo Zhang,Haiyang Hu,Mingxi Qiao,Dawei Chen,Xiuli Zhao
Amplifying “eat me signal” during tumor immunogenic cell death (ICD) cascade is crucial for tumor immunotherapy. Inspired by the indispensable role of adenosine triphosphate (ATP, a necessary “eat me signal” for ICD), a versatile ICD amplifier was developed for chemotherapy-sensitized immunotherapy. Doxorubicin (DOX), ATP and ferrous ions (Fe 2+ ) were co-assembled into nanosized amplifier (ADO-Fe) through π‒π stacking and coordination effect. Meanwhile, phenylboric acid-polyethylene glycol-phenylboric acid (PBA-PEG-PBA) was modified on the surface of ADO-Fe (denoted as PADO-Fe) by the virtue of d -ribose unit of ATP. PADO-Fe could display active targetability against tumor cells via sialic acid/PBA interaction. In acidic microenvironment, PBA-PEG-PBA would dissociate from amplifier. Moreover, high H 2 O 2 concentration would induce hydroxyl radical (·OH) and oxygen (O 2 ) generation through Fenton reaction by Fe 2+ . DOX and ATP would be released from the amplifier, which could induce ICD effect and “ICD adjuvant” to amplify this process. Together with programmed death ligands 1 (PD-L1) checkpoint blockade immunotherapy, PADO-Fe could not only activate immune response against primary tumor, but also strong abscopal effect against distant tumor. Our simple and multifunctional ICD amplifier opens a new window for enhancing ICD effect and immune checkpoint blockade therapy.
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Advanced Healthcare Materials
·
2023
·
IF 10
Jingyi Yang,Jiahao Qu,Xuanming Teng,Weiping Zhu,Yufang Xu,Yangyang Yang,Xuhong Qian
Most solid tumors are clinically treated using surgical resection, and the presence of residual tumor tissues at the surgical margins often determines tumor survival and recurrence. Herein, a hydrogel (Apt-HEX/Cp-BHQ1 Gel, termed AHB Gel) is developed for fluorescence-guided surgical resection. AHB Gel is constructed by tethering a polyacrylamide hydrogel and ATP-responsive aptamers together. It exhibits strong fluorescence under high ATP concentrations corresponding to the TME (100–500 µ m ) but shows little fluorescence at low ATP concentrations (10–100 n m ) such as those in normal tissues. AHB Gel can rapidly (within 3 min) emit fluorescence after exposure to ATP, and the fluorescence signal only occurs at sites exposed to high ATP, resulting in a clear boundary between the ATP-high and ATP-low regions. In vivo, AHB Gel exhibits specific tumor-targeting capacity with no fluorescence response in normal tissue, providing clear tumor boundaries. In addition, AHB Gel has good storage stability, which is conducive to its future clinical application. In summary, AHB Gel is a novel tumor microenvironment-targeted DNA-hybrid hydrogel for ATP-based fluorescence imaging. It can enable the precise imaging of tumor tissues, showing promising application in fluorescence-guided surgeries in the future.
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Colloid and Interface Science Communications
·
2025
·
IF 4.7
Qiaoyue Ren,Rui Wang,Bingfeng Wu,Dingming Huang,Ding Xiong,Yu Shi,Zhenming Wang
Effective wound healing remains a challenge in clinical medicine due to limitations in traditional dressings, which often lack the necessary biocompatibility, breathability and ability to provide bioenergy. In this study, we developed bioenergetic-active cryogels using adenosine salts (AMP 2Na, ADP-2Na, and ATP 2Na) as crosslinkers to enhance tissue regeneration through improved energy metabolism. The cryogels feature a porous and loose structure that facilitates water and gas exchange, as well as the absorption of wound exudate. Additionally, ATP-2Na-coordinated cryogel (G-ATP) stimulates cellular proliferation and migration, while exhibiting anti-inflammatory properties, thus creating an optimal environment for wound repair. In vivo experiments in a full-thickness wound model showed accelerated healing with the G-ATP group, which outperformed control groups in wound contraction and collagen deposition. These findings suggest that G-ATP serve as breathable physical barriers and bioactive dressings that enhance healing by supporting cellular energy needs. This novel approach provides a promising platform for advanced wound care, with potential applications in tissue engineering and regenerative medicine.
研究领域:
生物材料学
·
再生医学
·
伤口护理
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Catalysts
·
2025
·Vol. 15
·期 3
·252
·
IF 4
Haitao Zhang,Jian Shi,Chaoya Han,Zhizhao Song,Yao Xiao,Xiazhang Li
With the increasing demand for air pollution control, the development of efficient and stable catalysts to degrade hazardous VOCs such as toluene has become particularly important. Herein, various copper-doped attapulgite-supported cobalt oxide spinel composites (CuxCo3−xO4/ATP) were synthesized using an ultrasonic-assisted precipitation method. The results showed that the abundant Si-OH groups on the surface of ATP played a crucial role in anchoring Co, and the instantaneous high-energy input of ultrasonication facilitated the formation of Si-O-Co bonds in Co3O4/ATP. The doping of Cu ions induced the expansion of the Co3O4lattice, resulting in a significant number of oxygen vacancies. The ultrasound-induced synthesized Cu0.1Co2.9O4/ATP catalyst exhibited the best catalytic oxidation performance, achieving a 99% toluene degradation rate at 300 °C under a weight hourly space velocity (WHSV) of 10,000 mL·g−1h−1and initial toluene concentration of 1000 ppm, along with high stability during 12 h of continuous running. This work presents a new strategy for the cost-effective catalytic elimination of VOCs.
研究领域:
环境科学
·
催化材料
·
空气污染控制
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2018
·Vol. 186
·期 2
·467-473
·
IF 2.361
Cao Changyu,Luo Junchong,Li Xiaowen,Zhang Mengdan,Zhang Haoji,Zhang Jipei,Wang Kai
The aim of this study was to evaluate the protective effects of SeY (selenium-rich yeast) against Al (aluminum)-induced inflammation and ionic imbalances. Male Kunming mice were treated with Al (10 mg/kg) and/or SeY (0.1 mg/kg) by oral gavage for 28 days. The degree of inflammation was assessed by mRNA expression of inflammatory biomarkers. Ionic disorders were assessed by determining the Na + , K + , and Ca 2+ content, as well as the alteration in ATP-modifying enzymes (ATPases), including Na + K + -ATPase, Ca 2+ -ATPase, Mg 2+ -ATPase, Ca 2+ Mg 2+ -ATPase, and the mRNA levels of ATPase’s subunits in kidney. It was observed here that SeY exhibited a significant protective effect on the kidney against the Al-induced upregulation of pro-inflammatory and downregulation of anti-inflammatory cytokines. Furthermore, a significant effect of Al on the Na + , K + , Ca 2+ , and Mg 2+ levels in kidney was observed, and Al was observed to decrease the activities of Na + K + -ATPase, Mg 2+ -ATPase, and Ca 2+ Mg 2+ -ATPase. The mRNA expression of the Na + K + -ATPase subunits and Ca 2+ -ATPase subunits was regulated significantly by Al. Notably, SeY modulated the Al-induced alterations of ion concentrations, ATPase activity, and mRNA expression of their subunits. These results suggest that SeY prevents renal toxicity caused by Al via regulation of inflammatory responses, ATPase activities, and transcription of their subunits.
研究领域:
毒理学
·
营养学
·
分子生物学
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Min Long,Jiayang He,Xinyue Jia,Yuqing Zhang,Zhihan Zhang,Yaqin Chai,Ruo Yuan,Wenju Xu
To better balance background leakage and disassociation kinetics in traditional ligand-aptamer competition-based transduction, herein we explore the ratiometric fluorescence ability of red- and green-emissive Ag cluster ( r AgC and g AgC) that is illuminated by the adenosine triphosphate (ATP)-induced proximity hybridization. For proof-of-concept, an ATP-recognizable strand (AS) encoding the aptamer sequence (aS) in the middle and a stem-loop bi-template hairpin ( b TH) are introduced. Originally, the partial sequence for r AgC is blocked in the stem of hairpin-structured b TH, while tethering an exposed g AgC-clustering toehold for ‘green-on’ emission . Upon inputting ATP, the specific ATP-aptamer binding induces the dynamic switch of random-coil AS into stable hairpin structure (off-to-on transition) with a short-paired stem, in which two split invaders are oriented closely as a parent invader to interfere the unpaired loop of b TH. Through perfect proximity hybridization, rapid orthogonal DNA assembly urges b TH to lose its hairpin conformation geometry, thereby connecting the contiguous stem for preferable development of r AgC with ‘red-on’ emitting signal. Thus, ligand ATP and aptamer AS are like a logic ‘key/switch’ pair to execute an input-output connection. As predicted, the fluorescence intensity of r AgC and g AgC reversely changes in a tailored ATP-responsive route, obtaining the dose-dependent ratiometric response for sensing ATP. The conformation alteration by ligand-aptamer recognition would be more beneficial to guide precise proximity hybridization between two reactive species, constructing an applicable biosensing platform with simplification, specificity, low background and rapid reaction kinetics.
研究领域:
生物化学
·
分子生物学
·
分析化学
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