SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Xin Liu,Shiyue Niu,Liqian Niu,Xian Wang,Xinxin Liu,Xin Guan,Xinyu Fan,Shuyun Bi
A highly sensitive surface-enhanced Raman spectroscopy (SERS) approach was established for the detection of monosultap using the nanosubstrate of β -CD/Trp/MgO/AgNPs which was MgO-protected silver nanoparticles (AgNPs) assembled tryptophan (Trp) and β -cyclodextrin ( β -CD). Six nanosubstrates including AgNPs, MgO/AgNPs, Trp/AgNPs, β -CD/MgO/AgNPs, Trp/MgO/AgNPs and β -CD/Trp/MgO/AgNPs were compared for their enhancement effect on monosultap. Among them, β -CD/Trp/MgO/AgNPs had the greatest enhancement effect, with an enhancement factor (EF) reaching up to 1.95 × 10 6 . The nanosubstrate of β -CD/Trp/MgO/AgNPs was characterized by various instrumental techniques, such as transmission electron microscopy (TEM), high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), zeta potential, X-ray diffraction (XRD), thermogravimetric analysis (TGA), ultraviolet-visible (UV–vis) and Fourier transform infrared (FT-IR). The binding of β -CD/Trp/MgO/AgNPs with monosultap was studied in detail. The optimum experimental conditions were confirmed through single-factor experiments and response surface analysis. The interferences from the coexisting substances (common metal ions, glucose and congeneric drugs) were investigated to evaluate the method selectivity. Under the optimal conditions, the standard curve equation of monosultap was established within the concentration range of 2.00 × 10 −8 –1.00 × 10 −6 mol L −1 , with detection limit (LOD) of 0.970 nmol L −1 (3 σ / S ). The SERS approach was utilized for detecting monosultap in several vegetables and fruits with the recoveries from 96.3 % to 105 %.
研究领域:
分析化学
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纳米技术
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环境监测
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食品安全
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光谱学
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产品关联
DOI
PubMed
高影响因子
Chemical Engineering Journal Advances
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2021
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IF 0
Chao Liu,Cao Fang,Changsheng Shao,Xinxin Zheng,Hangbo Xu,Qing Huang
Currently, there is a huge demand for medical biomaterials aimed to improve antibacterial effect and promote wound healing. In this work, a facile and single-step approach of synthesizing silver nanoparticles (AgNPs) and reduced graphene oxide (rGO) composite material (namely, AgNPs@rGO) using high-voltage direct-current (DC) non-thermal plasma was proposed. Electron-beam (e-beam) from the DC-plasma discharge zone was implanted into the solution of AgNO 3 and graphene oxide (GO), leading to the reduction of both GO and silver ions. As a result, AgNPs were formed and distributed homogeneously on the reduced graphene sheets. This composite material exhibits excellent antibacterial performance, being able to kill both Gram-positive bacteria Staphylococcus aureus ( S. aureus ) and Gram-negative bacteria Escherichia coli ( E. coli ) effectively. Furthermore, this composite material was utilized to prepare wound-healing band-aids. The in vitro assay on mice confirmed the satisfactory curing effect. The cytotoxicity for bio-compatibility and the mechanism for sterilization were also examined and clarified. As such, this work puts forward a facile and new route of preparing antibacterial biomaterial and demonstrates its promising prospect in wound-healing application.
研究领域:
材料科学
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生物医学工程
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纳米医学
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DOI
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Jie Zhang,Xinru Wang,Jinyu Shan,Dong Yang,Guangming Tian,Yunzhi Dang,Jianhua Ma
Cellulose acetate (CA), an essential biocompatible polymer for medical dressings, requires integration with antimicrobial agents (e.g., antibiotics, silver nanoparticles) due to its inherent non-antimicrobial nature. While silver nanoparticles (AgNPs) offer broad-spectrum activity, their cytotoxicity caused by uncontrolled ion release remains a critical challenge. Herein, we report a green synthesis strategy using dopamine hydrochloride as both a reducing agent and interfacial modifier to in situ synthesize AgNPs on copper oxide (CuO) surfaces, achieving precise silver ion regulation through CuO-dopamine coordination. The optimized AgNPs@CuO composites were embedded into CA nanofibers via electrospinning, followed by the construction of a polycaprolactone (PCL) supporting layer to form a bilayer membrane. Remarkably, the PCL/CA/AgNPs@CuO membrane exhibited a significant increase in tensile strength compared to monolayer CA, while maintaining adequate porosity for optimal breathability. The CA/AgNPs@CuO nanofiber membrane achieved inhibition zone diameters of 3.9 mm and 4.7 mm against E. coli and S. aureus , respectively, and demonstrated continuous antimicrobial release over 36 h, ensuring long-term antibacterial efficacy. This hierarchically designed system provides a paradigm for developing robust antimicrobial dressings with balanced biocompatibility and durability.
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Yipeng Pang,Maofang Zhao,Yuhan Xie,Yanping Wang,Yuxin You,Yongding Ke,Chaoyi Zhang,Xiaohan Chen,Yijia Yang,Chunlei Zhang,Xi Chen,Yi Liu,Xingtang Fang
The development of non-antibiotic strategies for combating bacterial infections, particularly through reactive oxygen species (ROS) accumulation accompanying inflammation, has attracted much attention. Despite Allicin (Ac) emerges as a promising antimicrobial agent with wide and remarkable bioactivity, it still displays quite limited ROS scavenging and anti-inflammation capacity due to uncontrolled and poor bioavailability. Herein, a multifunctional nanoplatform with pH-dependent and ROS-scavenging antibacterial, anti-inflammatory and proangiogenic properties is introduced to promote infected wound healing by integrating Ac into silver nanoparticles (AgNPs)-loaded zeolite imidazole framework (ZIF-8), namely Ac@ZIF-8/AgNPs. Primarily, the nanoplatform is activated and controlled release in the infection microenvironment, with disrupting biofilms, scavenging ROS and antibacterial capacitys, outperforming conventional Ac. Moreover, in the mouse models induced by bacterial infection, Ac@ZIF-8/AgNPs can alleviate the inflammatory condition, scavenge ROS, facilitate the regeneration of collagen fiber and enhance vascular formation, collectively accelerating wound healing. Overall, this biocompatible nanoplatform is provided as a reliable approach to solve the long-standing problem of infected wound healing.
研究领域:
纳米技术
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生物医学工程
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药学
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伤口愈合
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DOI
高影响因子
TALANTA
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2024
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IF 5.6
Bin Yang,Jia Liu,Xin Liu,Shiyue Niu,Fengming Zhang,Yuting Wang,Daqian Song,Shuyun Bi
The high efficient surface-enhanced Raman scatterring (SERS) methods to detect thiacloprid and imidacloprid were established using ZIF-8-wrapped Ag nanoparticles (AgNPs) modified with β -cyclodextrin ( β -CD). The substrate of ZIF-8/ β -CD@AgNPs was characterized by ultraviolet visible spectra (UV–vis), thermogravimetric analysis (TGA), X-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). The interaction between the substrate and thiacloprid/imidacloprid was also explored. The optimum measurement conditions were obtained by response surface model based on single-factor experiments. Enhancement factors (EFs) of thiacloprid and imidacloprid were respectively 2.29 × 10 6 and 2.60 × 10 6 . A good linearity between the scattering intensity and the concentration of thiacloprid/imidacloprid within 3–1000 nmol L −1 /6–400 nmol L −1 was established. The interference experiments indicated that the methods had good selectivity . The SERS methods were successfully applied to detect thiacloprid and imidacloprid in several vegetables samples. The recoveries ranged from 95.5 % to 105 % (n = 5). The detection limits (LODs) (S/N = 3) for thiacloprid and imidacloprid were 1.50 and 0.83 nmol L −1 , respectively.
研究领域:
分析化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
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2025
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IF 2.5
Chen Yufeng,Li Yutong
A novel surface-enhanced Raman scattering (SERS) substrate was developed to determination of the pesticide carbendazim (CBZ) through the green synthesis of silver nanoparticles (SA-AgNPs-CD) using sodium alginate as a reducing agent and α-cyclodextrin as a molecular recognition stabilizer. The composite material was systematically characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and UV–vis spectroscopy. The analysis revealed spherical nanoparticles with an average diameter of approximately 59 nm and localized surface plasmon resonance at 412 nm. 4-aminothiophenol (PATP) was used as a SERS probe to discuss the SERS activity of the substrate and multivariate optimization of detection parameters (substrate–analyte ratio 3:1, pH 6.8, 15 min adsorption, and sequential reagent addition) via orthogonal experimental design. The linear response which extends from 5.0 × 10 −5 to 1.0 × 10 −7 mol/L for CBZ combined with principal component analysis (PCA) revealed an ideal linear relationship between CBZ concentration and principal component F 1 , achieving a limit of detection (LOD) of 1.95 × 10 −9 mol/L. It is expected that this SERS strategy will provide great value for rapid detecting pesticide residues in fruits and food products.
研究领域:
分析化学
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纳米技术
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环境科学
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食品安全
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
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2024
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IF 5.5
Zixuan Wang,Juan Peng,Houlin Li,Ganghui Chu
Luteolin is a variety of bioactive flavonoids, it is of great significance to quickly and accurately determine the content of luteolin. This paper established a simple and rapid ratiometric electrochemical sensor for the sensitive determination of luteolin. Nano-flowering zirconium-based metal-organic frameworks (UiO-66) were synthesized and silver nanoparticles (AgNPs) were directly grown on the surface of UiO-66 to prepare AgNPs@UiO-66 composites. Then, AgNPs@UiO-66 was drop-coated on the electrode surface to prepare the ratiometric electrochemical sensor. AgNPs@UiO-66 has the dual function of providing a stable internal reference signal and enhancing the electrochemical signal. Under optimal conditions, the peak current of luteolin (I Luteolin ) increased with increasing luteolin concentration, while the peak current of AgNPs@UiO-66 (I AgNPs@UiO-66 ), which is used as an internal reference signal, remained relatively stable. I Luteolin /I AgNPs@UiO-66 showed a good linear relationship in the range of 0.07 - 0.9 μM and 0.9 - 40 μM, and the limit of detection (LOD) was 0.017 μM (S/ N = 3). The sensor detected luteolin in peanut shells with recoveries ranging from 96.7 % to 107 %, which agreed with ultraviolet-visible spectroscopy. These results demonstrate the possibility of this ratiometric electrochemical sensor for detecting luteolin in real life.
研究领域:
分析化学
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植物学
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生物医学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Yijie Wang,Yue Huang,Yan Zhang,Henan Wang
Staphylococcal enterotoxins as common bacterial toxins can bring about food contamination and lead to severe foodborne illnesses, so facile and sensitive detection of staphylococcal enterotoxins is significant to ensure food safety and protect public health. In this work, an ultrasensitive biosensor for staphylococcal enterotoxin B (SEB) detection is developed based on the novel electrochemical nanoprobe named MXene/AgNPs@pSC4/MB, which is prepared by functionalizing MXene with para-sulfonatocalix[4]arene-decorated silver nanoparticles (AgNPs@pSC4) and electroactive signal molecules methylene blue (MB). The collaborative effect of AgNPs and MXene endows the fabricated electrochemical nanoprobe with excellent conductivity and admirable loading capacity of signal molecules to significantly enhance the response of biosensor, while pSC4 as the robust linker can bridge the nanoprobe and target SEB by binding with multifarious amino acid residues on SEB. With the specific aptamer immobilized on the electrode as the identifying element to capture SEB, the electrochemical nanoprobes MXene/AgNPs@pSC4/MB are anchored to SEB via pSC4, producing remarkably amplified signal output. The proposed electrochemical biosensor facilitates the ultrasensitive detection of SEB with a low detection limit of 1.76 × 10 −7 ng/mL in addition to the merits of facile operation and rapid response. Moreover, benefiting from the high chemical stability and good resistance to fouling and passivation of the electrochemical nanoprobe, the biosensor exhibits long-lasting stability, good accuracy and anti-interference ability in SEB detection. The reliable detectability of the biosensor has also been examined in the analysis of real food samples, demonstrating potential application toward food safety monitoring.
研究领域:
食品安全检测
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生物传感
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纳米技术
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微生物学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Lin Yang,Tingwang Guo,Bin Yao,Gang Chen
Highly sensitive and low-cost detection methods of cancer biomarker are very important for the early screening of cancer. In this contribution, a novel enzyme-free colorimetric immunoassay for sensitive and simple detection of alpha-fetoprotein (AFP) was developed based on the silver amplified strategy via graphene oxide (GO) as AgNPs carrier. AgNPs deposited on the surface of GO to form GO-AgNPs nanocomposites. GO-AgNPs nanocomposites were linked with detection antibody of AFP and quantitatively introduced by AFP through specific reaction between antigen and antibody. In the presence of H 2 O 2 , AgNPs dissolved and released Ag + . Ag + can promote coupling of 2,4-dichlorophenol with 4-aminoantipyrine to yield quinoneimine dye, by measuring the absorbance of the quinoneimine dye enzyme-free colorimetric immunoassay of AFP was established. The large surface area of GO can increase the introduction of AgNPs and greatly amplify the detection signal. The enzyme-free colorimetric immunoassay in this work with the advantages of cost effective, rapid detection and high sensitivity, and possesses excellent analytical performance of AFP in the range from 5 ng mL −1 to 200 ng mL −1 with a limit of detection of 0.19 ng mL −1 (3σ/k, n = 10). The results of human serum samples performance satisfactory, which demonstrated good application values for point of care testing (POCT) in clinical diagnosis.
研究领域:
生物医学工程
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临床诊断学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Tianyue Zhang,Xueting Li,Dongmei Liu,Jiyu An,Maofeng Zhang,Jian hua Li,Changlong Jiang
In-situ detection of analytes on curved surfaces is a persistent and great challenge in the application of SERS technique. A hydrogel-based flexible SERS substrate demonstrates a unique charm for providing close contact to the real-sample surface but limited by its weak enhancement capability in the quantitative analysis. Herein, we report a reinforced hydrogel by reduction of plasmonic silver nanoparticles (AgNPs) on a PNIPAM membrane with the aid of polyvinylpyrrolidone (PVP) surfactant. The modification of AgNPs on PNIPAM endows the AgNPs/PNIPAM composite hydrogel with good mechanical properties and reinforced swelling property. Moreover, the AgNPs/PNIPAM hydrogel-based SERS sensor exhibits an enhanced SERS sensitivity as well as improved signal uniformity (RSD ＜10 %), and the detection concentration for pesticide molecules can be down to 10 -9 M. Due to the fingerprint feature of SERS, multicomponent detection of pesticide mixtures was readily realized. In addition, the flexible AgNPs/PNIPAM hydrogel was successfully applied to the curved apple surface for SERS detection. Notably, it is capable of achieving qualitative and quantitative analyses of different pesticides, which has lower analytical concentrations than European Union (EU) standards, indicating the potential applications in rapid in-situ pesticide analysis.
研究领域:
分析化学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
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2025
·
IF 6.5
Tiantian Liu,Feifei Xie,Lele Geng,Ruizhe He,Mengzhe Sun,Tao Ni,Peng Xu,Chao Xing,Yinbo Peng,Ke Chen,Yong Fang
Purpose The purpose of this study is to develop an innovative solution for chronic wounds in high-mobility areas, such as joints, where conventional treatments are hindered by passive healing mechanisms and the need for immobilization. By designing a micro-electro-Nanofiber dressing composed of piezoelectric polyvinylidene fluoride (PVDF) integrated with antimicrobial silver nanoparticles (AgNPs), this research aims to address the dual challenges of promoting effective wound healing and maintaining joint mobility.Methods Herein, we developed a novel micro-electro-Nanofiber dressing using electrospinning technology, incorporating polyvinylidene fluoride (PVDF) with silver nanoparticles (AgNPs). The optimized PVDF-AgNPs Nanofiber dressings exhibited strong piezoelectric effects suitable for joint wounds.Results In vitro experiments demonstrated that the dressing effectively promoted fibroblast migration and collagen synthesis. In vivo, the dressing exhibited a trend of rapid healing in infected wounds within 12 days while modulating macrophage differentiation toward the anti-inflammatory M2 phenotype. Additionally, the incorporation of antimicrobial nanosilver effectively controlled local infections, further facilitating the healing process.Conclusion To sum up, by harnessing the piezoelectric effect to stimulate endogenous healing mechanisms without restricting joint mobility, the developed PVDF-AgNPs Nanofiber dressings represent a transformative approach for the treatment of wounds in highly mobile body areas.
研究领域:
生物医学工程
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材料科学
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伤口护理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS A-PHYSICAL
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2024
·
IF 4.1
Chaoying Wang,Chuangao Ma,Zhuping Han,Mengnan Li,Shaoning Lu,Peibang Dai
Highly sensitive sensors have attracted considerable attention in areas such as electronic skin, health monitoring and artificial intelligence. Herein, a simple and efficient approach was proposed to fabricate hierarchically structured flexible sensors. A common metal mesh was used as a template and its pattern was transferred to the surface of a composite film of silver nanoparticles (AgNPs) decorated polydopamine (PDA) and polyurethane (TPU) electrospun fibers by hot imprinting. The resulting film with a uniform convex microstructure array on the surface was used as the conductive sensing layer of the AgNPs/PDA/TPU sensor. The sensor exhibited a linear detection range increased from 15 kPa to 20 kPa compared to the control sensor without the microstructure. Furthermore, the sensitivity was improved from 8.13 kPa −1 to 10.44 kPa −1 . The response time and recovery time of the sensor were determined to be 110 ms and 90 ms, respectively, and it maintained good stability at 10 kPa even after 1000 compression cycles. In addition, the sensor enabled real-time pressure signal detection for touch, joint movement and voice recognition, showing promising applications in health monitoring, pressure detection and human-machine interaction.
研究领域:
材料科学
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生物医学工程
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电子皮肤技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）