Nutrients
·
2022
·Vol. 14
·期 13
·2768
·
IF 6.706
Di Chen,Jiyong Yin,Zhuo Yang,Wen Qin,Junsheng Huo,Jian Huang,Jing Sun,Wei Piao
The current cytological evaluation technique of health food raw materials does not entirely meet the needs of evaluating health food. Our study adopted the microfluidic chip technique for the first time to construct a hepatocyte model of evaluating emodin, which was composed of a human hepatocellular carcinoma cell (HepG2) and microfluidic chip. The mixed glue of a model with rat tail collagen type I (1.3 mg/mL) + gelatin (7.5%) was used to simulate the microenvironment of a cell. The validity of this model was evaluated by cell proliferation activity and cell staining, and the toxicity of emodin was evaluated by a series of metabolic indicators on this model. The results indicated that the repeatability of the constructed hepatocyte model was favorable, with a coefficient of variation (CV) of 2.8%. After emodin continuously was exposed for 48 h, the cell inhibition was obvious at 100 and 200 μM, and the number of dead cells gradually increased with the increasing of emodin concentration, and the difference of BUN was significant between the emodin group and blank group (p< 0.05). The constructed model has a favorable applicability in evaluating emodin. This study provides an important platform and a potential in vitro alternative model for assessing and predicting the health effects of health food.Keywords:microfluidic chip;raw materials of health food;emodin;hepatocyte model;evaluation
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Micromachines
·
2021
·Vol. 12
·期 9
·1017
·
IF 2.891
Zhenhu Wang,De Gong,Jun Cai
Diatom frustules are a type of natural biomaterials that feature regular shape and intricate hierarchical micro/nano structures. They have shown excellent performance in biosensing, yet few studies have been performed on flow-through detection. In this study, diatom frustules were patterned into step-through holes and bonded with silicon substrate to form an open-ended filtration array. Then they were fixed into a microfluidic chip with a smartphone-based POCT. Human IgG and FITC-labeled goat–anti-human IgG were adopted to investigate the adsorption enhancement when analyte flowed through diatom frustules. The results indicated up to 16-fold enhancement of fluorescent signal sensitivity for the flow-through mode compared with flow-over mode, at a low concentration of 10.0 μg/mL. Moreover, the maximum flow rate reached 2.0 μL/s, which resulted in a significant decrease in the testing time in POCT. The adsorption simulation results of diatom array embedded in the microchannel shows good agreement with experimental results, which further proves the filtration enrichment effect of the diatom array. The methods put forward in this study may open a new window for the application of diatom frustules in biosensing platforms.Keywords:diatom frustules;array;flow-through;microfluidic chip;POCT;biosensing
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Min Guo,Xiao-Qing Yu,Jin Zhao,Jia-Wei Wang,Ren-Kun Qing,Ji-Dong Liu,Xingjiang Wu,Liangliang Zhu,Su Chen
Photonic crystals with orderly periodic structure and manipulated structural colors have garnered widespread attention in sensors field. However, the unsatisfactory detection range, low sensitivity and inconvenient integration highly limit their further practical applications. In this paper, by combining TiO 2 inverse opal photonic crystal with polyacrylamide hydrogel, a novel organic-inorganic composite solvents responsive photonic hydrogel (SRPH) was developed. By encapsulating TiO 2 inverse opal photonic crystal with high refractive index in this hydrogel, the photonic hydrogels, therefore, possess bright structural colors. Due to the discrepancy in swelling rate of SRPH film in various solvents (ethanol, dimethyl formamide , dimethyl sulfoxide , etc.), it can identify these organic solvents through the rapid change of structural colors and detect the concentrations of the solvent in a wide range. Even for solvents with similar refractive index, it can be clearly distinguished, along with the excellent repeatability. Benefiting from the wide detection range and high sensitivity, the chip sensors were then developed by integrating multiple hydrogel films into the microfluidic system, which have high efficiency in solvent naked-eye multi-identification and concentration multi-detection. The new composite photonic hydrogels provide a reference for the design of functional photonic crystals and next-generation application of sensors .
✓ 已验证
产品关联
DOI
高影响因子
Acta Biomaterialia
·
2021
·
IF 8.947
Kun Du,Shibo Li,Chengpan Li,Ping Li,Chunguang Miao,Tianzhi Luo,Bensheng Qiu,Weiping Ding
Nonalcoholic fatty liver disease (NAFLD) has emerged as a public health concern. To date, the mechanism of NAFLD progression remains unclear, and pharmacological treatment options are scarce. Traditional animal NAFLD models are limited in helping address these problems due to interspecies differences. Liver chips are promising for modeling NAFLD. However, pre-existing liver chips cannot reproduce complex physicochemical microenvironments of the liver effectively; thus, NAFLD modeling based on these chips is incomplete. Herein, we develop a biomimetic liver lobule chip (LC) and then establish a more accurate on-chip NAFLD model. The self-developed LC achieves dual blood supply through the designed hepatic portal vein and hepatic artery and the microtissue cultured on the LC forms multiple structures similar to in vivo liver. Based on the LC, NAFLD is modeled. Steatosis is successfully induced and more importantly, changing lipid zonation in a liver lobule with the progression of NAFLD is demonstrated for the first time on a microfluidic chip. In addition, the application of the induced NAFLD model has been preliminarily demonstrated in the prevention and reversibility of promising drugs. This study provides a promising platform to understand NAFLD progression and identify drugs for treating NAFLD. Statement of significance Liver chips are promising for modeling nonalcoholic fatty liver disease. However, on-chip replicating liver physicochemical microenvironments is still a challenge. Herein, we developed a liver lobule chip with dual blood supply, achieving self-organized liver microtissue that is similar to in vivo tissue. Based on the chip, we successfully modeled NAFLD under physiologically differentiated nutrient supplies. For the first time, the changing lipid zonation in a single liver lobule with the early-stage progression of NAFLD was demonstrated on a liver chip. This study provides a promising platform for modeling liver-related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LAB ON A CHIP
·
2020
·Vol. 20
·期 7
·1227-1237
·
IF 6.774
Chang Chen,Dong Xu,Siwei Bai,Zhihang Yu,Yonggang Zhu,Xiao Xing,Huaying Chen
Inoculation of single cells into separate culture chambers is one of the key requirements in single-cell analysis. This paper reports an innovative microfluidic chip integrating two pneumatic microvalves to screen and print single cells onto a well plate. The upper and lower size limits of cells can be dynamically controlled by regulating the deformation of two adjacent microvalves. Numerical simulations were employed to systematically study the influence of membrane dimensions and pressure on the deflection of a valve. A mathematical model was then modified to predict the size of cells captured by a microvalve at various pressures. The membrane deflection was further studied using confocal imaging. The critical pressure trapping beads of various sizes was experimentally determined. These experiments validated the accuracy of both numerical simulations and the mathematical model. Furthermore, single beads and endothelial cells with the desired size range were screened using dual valves and printed onto well plates with 100% efficiency. Viability studies suggested that the screening process had no significant impact on cells. This device enables dynamic regulation of both the lower and the upper size limits of cells for printing. It has significant application potential in inoculating cells with desired sizes for various fields such as clonal expansion, monoclonality development and single-cell genomic studies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 42
·5413-5420
·
IF 2.378
Jiawei Li,Ping Yang,Rui Huang,Danqun Huo,Changjun Hou
An accurate, effective, and convenient method for identification of Baijiu (a distilled liquor popular in China) components could be important for its commercial management. Herein, we report, for the first time, a fluorescence-amplifying sensor array chip based on photonic crystals (PCs) employed to identify Baijiu components. Transmission electron microscopy and scanning electron microscopy were employed to characterize polystyrene microspheres and PCs, which exhibited characteristic uniform and compact arrangements. A spotting machine and fluorescence scanner were used for dye loading and data collection. Array performance was evaluated by pattern recognition and neural-network simulation. Our array chip was manufactured successfully and revealed outstanding discriminative ability for Baijiu. Compared with other liquid sensor arrays used only for configuration, our array chip possessed >90% leave-one-out accuracy after 10 days of dry storage under a N2 atmosphere. This simple and accurate method shows potential for quality control and detection in factories/markets.
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2019
·
IF 6.944
Xiaomin Yue,Yan Su,Xinnan Wang,Linfang Li,Wei Ji,Yukihiro Ozaki
An innovative ratiometric surface-enhanced Raman scattering (SERS) sensor using a 4-mercaptoboric acid (4-MPBA)-modified silver nanoparticle-decorated silicon wafer (Si@Ag NPs chip) was proposed for the ultrasensitive determination of F– ions in aqueous solutions. The principle of sensing strategy is based on fluoride-induced structural symmetry breaking and charge redistribution of phenylboronic acid, leading to a band shift of the C–C stretching mode of 4-MPBA from 1589 to 1576 cm–1. Accordingly, a ratiometric signal of the area ratio (A1576/A1589) between the fluoride-bond MPBA molecules and unoccupied MPBA molecules can be used for the quantitative response of F– ions. In comparison with other SERS-based sensing methods, this ratiometric method can avoid a large error resulting from the inhomogeneity of substrates. Under the optimized analytical conditions, the proposed SERS sensor possesses a quick response to F– ions within 2 min and exhibits high selectivity for F– ions with the determination limit of 10–8 M, which is over 3 orders of magnitude lower than the World Health Organization (WHO) guideline value for F– ions in drinking water. Of particular significance, the present sensor features favorable recyclability, which preserves suitable reproducibility during 6-time cyclic determination of F– ions. The practical utility of this sensing system for the determination of F– ions was tested with real water and toothpaste samples, and the results demonstrate that this sensor shows high recoveries (90–110%). Given its simple principle and easy operation, the present silicon-based SERS sensor could serve as a promising sensor for various practical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Zhaoyan Tian,Li Mi,Yafeng Wu,Fengying Shao,Mingqiang Zou,Zhenxian Zhou,Songqin Liu
This work reports an electrofluorochromic strategy on the basis of electric field control of fluorescent signal generation on bipolar electrodes (BPEs) for visualizing cancer cell surface glycoprotein (mucin 1). The device included two separate cells: anodic sensing cell and cathodic reporting cell, which were connected by a screen-printing electrode patterned on poly(ethylene terephthalate) (PET) membrane. In the sensing cell, anti-MUC1 antibody immobilized on a chitosan-multiwalled carbon nanotube (CS-MWCNT)-modified anodic BPE channel was used for capturing mucin-1 (MUC1) or MCF-7 cancer cells. Then ferrocene (Fc)-labeled mucin 1 aptamers were introduced through hybridization. Under an applied voltage, the ferrocene was oxidized and the electroactive molecules of 1,4-benzoquinone (BQ) in the cathodic reporting cell were reduced according to electroneutrality. This produced a strongly basic 1,4-benzoquinone anion radical (BQ•–), which turned on the fluorescence of pH-responsive fluorescent molecules of (2-(2-(4-hydroxystyryl)-6-methyl-4H-pyran-4-ylidene)malononitrile) (SPM) coexisting in the cathode reporting cell for both spectrophotometric detection and imaging. This strategy allowed sensitive detection of MUC1 at a concentration down to 10 fM and was capable of detecting a minimum of three MCF-7 cells. Furthermore, the amount of MUC1 on MCF-7 cells was calculated to be 6.02 × 104 molecules/cell. Our strategy also had the advantages of high temporal and spatial resolution, short response time, and high luminous contrast and is of great significance for human health and the promotion of life science development.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Micromachines
·
2021
·Vol. 12
·期 9
·1016
·
IF 2.891
Zhou Zhou,Manman Xu,Chenlin Zhu,Gonghan He,Kunpeng Zhang,Daoheng Sun
A control chip with a multistage flow-rate regulation function based on the correlation between the flow resistance and flow rate has been developed in this article. Compared with the traditional proportional solenoid valve, this kind of flow valve based on microfluidic technology has the characteristics of being light-weight and having no electric drive. It solves such technical problems as how the current digital microfluidic chip can only adjust the flow switch, and the adjustment of the flow rate is difficult. To linearize the output signal, we propose a design method of weighted resistance. The output flow is controlled by a 4-bit binary pressure signal. According to the binary value of the 4-bit pressure signal at the input, the output can achieve 16-stage flow adjustment. Furthermore, we integrate the three-dimensional flow resistance network, multilayer structure microvalve, and parallel fluid network into a single chip by using 3D printing to obtain a modular flow control unit. This structure enables the microflow control signal to be converted from a digital signal to an analogue signal (DA conversion), and is suitable for microflow driving components, such as in microfluidic chip sampling systems and proportional mixing systems. In the future, we expect this device to even be used in the automatic control system of a miniature pneumatic soft actuator.Keywords:microfluidic;soft robotics;hydraulic actuators;analog circuits
研究领域:
微流体技术
·
机器人学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Zhenyong Dong,Feixiang Zheng,Hao Wang,Yukai Liu,Xiao Ren,Feng Lin,Song Zhou,Li Chen,Yan-qing Lu,Guanghui Wang
The use of multiple pesticides in agriculture has caused mixed residues, complicating food safety testing. Surface-enhanced Raman scattering (SERS) offers high sensitivity and fingerprinting capabilities for pesticide detection; however,its application is limited by spot size, uneven sample distribution, spectral analysis challenges in mixed samples, and the lack of integrated automated systems for sample preparation, detection, and analysis. Hence, we developed a SERS microfluidic chip integrated with plasmonic microsphere lens arrays, which integrates optical field-modulated microspheres and plasmonic nanostructures to realise the transition of SERS detection from point-hotspots to uniform area-hotspots, achieving high-sensitivity, high-stability Raman detection under wide-field laser excitation. Moreover, a comprehensive a sample-to-answer total analysis system was established with this chip as the core, incorporating the micro-QuEChERS pretreatment module for efficient extraction of pesticides in complex matrices and the random forest-dual annealing spectral parsing algorithm for mixed spectral analysis. The results indicate that the limits of detection for 2,4-dichlorophenoxyacetic acid, acetamiprid, and thiabendazole were 1.15 nM, 0.63 nM, and 0.69 nM, respectively. The linear ranges were 10⁻⁴–10⁻⁹ M, with R² values of 0.9982, 0.9989, and 0.9996, respectively. Average recoveries ranged from 90.8 % -104.66 %, 89.1 %-107.89 %, and 89.73 %-108.4 %, respectively, with relative standard deviations below 8 %. The algorithm quantified mixed pesticides with mean absolute percentage errors of 4.96 %, 5.35 %, and 7.72 %, respectively. The system showed 3 s detection response and 15-minute analysis time. Thus, the method provides a rapid, on-site solution for detecting mixed pesticides in complex matrices by exceptional optical performance and automated processing functionality.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Chemistry
·
2019
·
IF 1.727
Hao Zhou,Yilin Song,Zheng Yang
A coupled chip aiming at economical and highly selective ammonium detection was fabricated. It consisted of a reaction chip, a gas-diffusion chip, and a detection chip. Zinc tetraphenylporphyrin dyed on the cation-exchange resin microbeads was used as the indicating material to avoid excess consumption for its reversibility. PDMS was selected as the material of the gas-diffusion membrane. A portable spectrometer was applied for spectrum analysis. By analysis of spectrum change, the high selectivity was confirmed because no component had interference on detection effect. Good performance was shown for all the tested concentrations (0.2–50 mg·L−1). The stability and reversibility were also judged by the spectrum data obtained from the indicating process and the recovering process. Finally, real samples containing ammonium were tested and the results were compared to those came from a standard method to confirm the accuracy of our method.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
Plasmonics
·
2015
·Vol. 11
·期 4
·1119-1128
·
IF 2.238
Pang Kai,Dong Wei,Zhang Bing,Zhan Shuyue,Wang Xiaoping
We demonstrate that a designed bimetallic chip is capable of improving the performance of a surface plasmon resonance (SPR) sensor based on angular interrogation. Through a numerical simulation and a refractometry experiment, we prove that this bimetallic chip can effectively reduce the noise level by about a factor of 2 compared to the traditional SPR sensors that only use a single gold film. The bimetallic chip presents a lower refractive index resolution of 5.3 × 10 −7 refractive index units. In addition, the enhancement of the electric field intensity at the surface of the configuration by a factor of 2 makes it possible to have a high sensitivity in a larger region, which promotes the biosensing applications of the chip. Through a simple and novel method for the detection of cadmium ions (Cd 2+ ) based on the bimetallic configuration, a detection level for Cd 2+ (0.01 μM or 1.12 ppb) can be realized, which compares favorably with similar studies.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）