SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Sibo Zhao,Huilin Wen,Yangteng Ou,Minhui Li,Lancheng Wang,Huimin Zhou,Bin Di,Ziyi Yu,Chi Hu
Biosensors integrating genetically programmed living cells into hostile and synthetic environments ( e.g. , devices or matrices) provide powerful tools for scientific research and enable new technological applications. However, it remains a challenge to maintain the viability and functionality of the living components exposed to different chemical, physiological or mechanical conditions in specific applications. To this end, we report herein a microfluidic approach to encapsulate microorganisms in gel-shell beads (GSBs) as a novel kind of biosensor. Inspired by biosilicification, hydrogel beads are coated with a mesoporous shell that can prevent cell leakage and afford mechanical stability, with the porous hydrogel structure naturally providing sustained supplies of water and nutrients to the embedded microorganisms. Communications between compartmentalized bacterial strains and the environment is achieved through the diffusion of signalling molecules across the mesoporous shell. Application of GSBs as living cell-based sensors is exemplified in a quorum sensing system, where genetically engineered Escherichia coli cells are encapsulated in GSBs and can respond to autoinducer concentrations ranging from 10 −6 to 10 −7 mol/L as secreted by human pathogen Pseudomonas aeruginosa in the infection site.
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高影响因子
PLoS One
·
2013
·Vol. 8
·期 9
·e74100
·
IF 3.73
Heyu Chen,Yao Wang,Jixi Zhang,Yingxin Ma,Caixia Wang,Ying Zhou,Hongchen Gu,Weihai Ying
Aim
To test the hypothesis that NAD + -carrying mesoporous silica nanoparticles (M-MSNs@NAD+) can effectively deliver NAD + into cells to produce cytoprotective effects.
Methods & Materials
NAD + was incorporated into M-MSNs. Primary rat astrocyte cultures and PC12 cells were treated with H 2 O 2 , followed by post-treatment with M-MSNs@NAD+. After various durations of the post-treatment, intracellular NAD + levels, intracellular ATP levels and lactate dehydrogenase (LDH) release were determined.
Results & Discussion
M-MSNs can be effectively loaded with NAD + . The M-MSNs@NAD+ can significantly attenuate H 2 O 2 -induced NAD + and ATP decreases in both astrocyte cultures and PC12 cells. M-MSNs@NAD+ can also partially prevent the H 2 O 2 -induced LDH release from both astrocyte cultures and PC12 cells. In contrast, the NAD + that is spontaneously released from the M-MSNs@NAD+ is insufficient to prevent the H 2 O 2 -induced damage.
Conclusions
Our study has suggested the first approach that can effectively deliver NAD + into cells, which provides an important basis both for elucidating the roles of intracellular NAD + in biological functions and for therapeutic applications of NAD + . Our study has also provided the first direct evidence demonstrating a key role of NAD + depletion in oxidative stress-induced ATP decreases.
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高影响因子
FISH & SHELLFISH IMMUNOLOGY
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2017
·
IF 3.148
Lei Liu,Xiao Tu,Yu-Feng Shen,Wei-Chao Chen,Bin Zhu,Gao-Xue Wang
Different viruses could induced ROS generation to alter intracellular redox state in the host cells, and unbalanced redox state was suggested to have various effects on viral replication. In this study, we investigated the influence of reactive oxygen species (ROS) on replication of spring viraemia of carp virus (SVCV) in fish cells. After SVCV infection, there existed a time-dependent increase in ROS generation. The present results revealed that antioxidant N-acetyl- l -cysteine (NAC) resulted in a lower ROS levels and increased SVCV replication in EPC cell. In contrast, a GSH synthesis inhibitor buthionine sulfoximine (BSO) induced ROS generation and decreased SVCV replication. In addition, activation of NF-κB suppressed SVCV replication by using two inhibitors of cytokine-induced IκBα phosphorylation. More importantly, enhancement of the activity of NF-κB was found in BSO treatment, which indicated that dropped SVCV replication likely occurred via ROS activation of NF-κB. Overall, our results revealed that the SVCV infection and replication could generate ROS and be affected by the redox state, where this progression was associated with the alteration in NF-κB pathway induced by oxidative stress .
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STEROIDS
·
2011
·
IF 3.106
Long Ji,Yonglei Yuan,Liping Luo,Zhe Chen,Xiaoqiong Ma,Zhongjun Ma,Lin Cheng
Michael reaction acceptors (MRAs) are a class of active molecules that are directly or indirectly involved in various cellular processes, including the regulation of many signaling pathways . In this study, the inducible nitric oxide synthase (iNOS) assay was used to demonstrate that the dichloromethane extract of Physalis alkekengi var . franchetii (DCEP) possesses anti-inflammatory activity that might be attributed to the modification of key cysteine residues in IKKβ by the MRAs in DCEP. To isolate these MRAs, glutathione (GSH) was employed, and a simple ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) screening method was developed to investigate the GSH conjugates with potential MRAs. Five physalins, including one new compound isophysalin A ( 2 ), together with four known steroidal compounds, physalin A ( 1 ), physalin O ( 3 ), physalin L ( 4 ) and physalin G ( 5 ), were isolated to evaluate the GSH conjugating abilities, and it was indicated that compounds 1 , 2 and 3 , which had a common α,β-unsaturated ketone moiety, exhibited conjugating abilities with GSH and also showed significant nitric oxide (NO) production inhibiting activities. The anti-inflammatory activities of compounds 1 , 2 and 3 might be attributed to their targeting multiple cysteine residues on IKKβ; therefore, the alkylation of IKKβ by compound 1 was further studied by micrOTOF-MS. The result showed that six cysteine residues (C 59 , C 179 , C 299 , C 370 , C 412 , and C 618 ) were alkylated, which indicated that IKKβ is a potential target for the anti-inflammatory activity of physalin A.
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Frontiers in Sustainable Food Systems
·
2025
·
IF 3.1
Huifang Liu,Jianmei Yao,Rongxiu Yin,Chiyu Ma,Lulu Li,Wen Yang,Zhiwei Lei
Introduction: Tea gray blight disease is a major leaf disease in tea plants, significantly reducing tea quality and yield. Plants are rich in bioactive compounds that are safe, non-toxic, and biodegradable. In this study, we evaluated the antifungal activities and mechanisms of 11 plant extracts against N. ellipsospora.Methods: This study evaluated the antifungal activity of 11 plant extracts against N. ellipsospora of tea gray blight disease and systematically examined the impacts of osthole and carvacrol on the mycelial morphology, mycelial weight, cell microstructure, membrane permeability, various biochemical substrate levels and related gene expression levels of N. ellipsospora.Results: The results showed that carvacrol and osthole exhibited significant antifungal effects among 11 plant extracts with EC50 values of 24.40 and 9.38 mg/L, respectively. Further research demonstrated that carvacrol and osthole significantly inhibited mycelial growth, reduced lesion areas on tea leaves, and markedly affected mycelial morphology and ultrastructure. Observations of mycelial morphology and ultrastructure revealed that carvacrol and osthole caused shrinkage and distortion of the mycelial surface, damage to cell wall and membrane, and disorganization of cellular organelles. Particularly carvacrol and osthole significantly increased chitinase activity, inhibited β-1,3-glucanase activity, and regulated the expression of genes encoding these enzymes.Discussion: The findings indicated that carvacrol and osthole could inhibit the growth of N. ellipsospora by disrupting the integrity of the cell wall and membrane and interfere with energy metabolism. This study would provide a theoretical basis for the development of novel plant-based fungicides.
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Biomed Research International
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2018
·
IF 2.583
Wenjing Sun,Yan Guo,Shirong Zhang,Zhihui Chen,Kangqi Wu,Qin Liu,Kaijun Liu,Liangzhi Wen,Yanling Wei,Bin Wang,Dongfeng Chen
Aim: We tested the hypothesis that fecal microbiota transplantation (FMT) could regulate the biotransformation of bile acids, such as deoxycholic acid (DCA) and cholic acid (CA), which in turn regulate the biosynthesis of serotonin in the gut and relieve gastrointestinal dysmotility in high-fat diet- (HFD-) induced obesity in rats. Methods: Male Sprague-Dawley rats were randomly divided into the control diet group, HFD group, and HFD-fed with receiving FMT. HFD was fed for 12 weeks. At the end of two-week HFD, FMT was carried out for two weeks. The gastrointestinal transit, serotonin concentration, the expression of tryptophan hydroxylase 1 (TPH1) and serotonin reuptake transporter (SERT), and the levels of bile acids in intestinal contents were examined. Results: Compared with the control group, the gastrointestinal transit and small intestinal serotonin concentration of HFD-fed rats were increased. In HFD-fed rats, TPH1 protein expression was increased significantly, while SERT protein expression was decreased, but not significant. The levels of CA and DCA in intestinal contents were also significantly increased in HFD-fed rats compared with the control group. After HFD-fed rats receiving FMT treatment, the gastrointestinal transit, small intestinal serotonin concentration, and TPH1 expression were decreased, while SERT expression was not affected. Moreover, the levels of CA and DCA in intestinal contents were also decreased. Conclusions: FMT could alleviate small intestinal transit in the HFD-fed rats by regulating the serotonin biosynthesis. In this process, CA and DCA may be related to the regulation of synthesis of serotonin.
研究领域:
胃肠病学
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微生物学
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营养学
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药理学
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PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICAL RESEARCH
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2025
·Vol. 30
·期 1
·1-14
·
IF 3.4
Wang Xiu,Du Wentao,Wang Yunyun,Khan Ahsan Riaz,Zhang Haijun
Objective This study aims to design and fabricate a leadless pacemaker (LPM) electrode loaded with rapamycin (RAPA)–poly(lactic-co-glycolic acid) (PLGA) drug sustained-release system to reduce the local fibrous tissue proliferation after LPM implantation, reduce local bioelectrical impedance, and facilitate the safe extraction of LPM after use. Methods We fabricated an LPM electrode loaded with the RAPA-PLGA drug-sustained-release system and carried out in vitro and in vivo experiments to verify its effect. Results A scanning electron microscope showed that the LPM electrode cavity was loaded with the RAPA-PLGA drug’s sustained-release system. The energy-dispersive spectrometer showed that the LPM electrode had RAPA and PLGA-related elements. The average drug loading rate of the drug sustained-release system was (51.02% ± 2.66) %, and the encapsulation rate was (85.04% ± 4.43%). The RAPA loaded in the electrode chamber was about (337.83 ± 53.66)μg. In vitro release results show that the LPM electrode loaded with RAPA–PLGA can continue to release for 44 days. In vitro cell inhibition experiments showed that the drug-loaded electrode group had an obvious inhibitory effect on fibroblasts, and the difference between the groups was significant ( p < 0.05). In vivo experiments showed that the local bioelectrical impedance of the drug-loaded electrode group is lower than that of the control group, with a difference between groups with statistical significance ( p < 0.05). The histopathological analysis of tissue sections from the site of (LPM electrode implantation revealed reduced fibrous tissue hyperplasia in the drug-loaded electrode group compared to the control group. Additionally, H&E staining indicated that the implantation of drug-loaded electrodes did not induce abnormal alterations in the liver, heart, spleen, lung, or kidney tissues. Conclusion The LPM electrode loaded with RAPA–PLGA demonstrates significant, sustained drug release and anti-proliferative effects in vitro. This drug-loaded electrode has been deemed safe for implantation in animal models. It can effectively inhibit local fibrous tissue proliferation and reduce local bioelectrical impedance, offering a technical strategy to prolong the in vivo functionality of LPMs and enhance clinical procedures.
研究领域:
医学工程
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药理学
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心血管病学
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高影响因子
JOURNAL OF PHYSICAL CHEMISTRY A
·
2024
·
IF 2.7
Alexander M. Mebel,Wang Li,Luna Pratali Maffei,Carlo Cavallotti,Alexander N. Morozov,Chang-Yang Wang,Jiu-Zhong Yang,Long Zhao,Ralf I. Kaiser
To understand the reactivity of resonantly stabilized radicals, often found in relevant concentrations in gaseous environments, it is important to determine their main reaction pathways. Here, it is investigated whether the fulvenallenyl radical (C7H5·) reacts preferentially with closed-shell molecules or radicals. Electronic structure calculations on the C10H9 potential energy surface accessed by the reactions of C7H5· with methylacetylene (CH3CCH) and allene (H2CCCH2) were combined with RRKM-ME calculations of temperature- and pressure-dependent rate constants using the automated EStokTP software suite and kinetic modeling to assess the reactivity of C7H5· with closed-shell unsaturated hydrocarbons. Experimentally, the reactions were attempted in a chemical microreactor heated to 998 ± 10 K by preparing fulvenallenyl radicals via pyrolysis of trichloromethylbenzene (C7H5Cl3) and seeding the radicals in methylacetylene or allene carrier gas, with product identification by means of photoionization mass spectrometry. The measured photoionization efficiency curve of m/z = 128 was assigned to a linear combination of the reference curves of two C10H8 isomers, azulene (minor) and naphthalene (major), presumably resulting from the C7H5· plus C3H4 reactions. However, the calculations demonstrated that these reactions are too slow, and kinetic modeling of processes in the reactor allowed us to conclude that the observation of naphthalene and azulene is due to the C7H5· plus C3H3· reaction, where propargyl is produced by direct hydrogen atom abstraction by chlorine (Cl) atoms from allene or methylacetylene and Cl stem from the pyrolysis of C7H5Cl3. Modeling results under the copyrolysis conditions of toluene and methylacetylene in high-temperature shock tube experiments confirmed the prevalence of the fulvenallenyl reaction with propargyl over its reactions with C3H4 even when the concentrations of allene and methylacetylene largely exceed that of propargyl. Overall, the reactions of fulvenallenyl with both allene and methylacetylene were found to be noncompetitive in the formation of naphthalene and azulene thus attesting the inefficiency of the fulvenallenyl radical reactions with the prototype closed-shell hydrocarbon species. In the meantime, the new reaction pathways revealed, including H-assisted isomerizations between C10H8 isomers and decomposition reactions of various C10H9 isomers, emerge as relevant and are recommended for inclusion in combustion kinetic models for naphthalene formation.
研究领域:
物理化学
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燃烧科学
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反应动力学
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理论化学
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质谱分析
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高影响因子
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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2021
·
IF 3.636
Feng-Ying Gao,Xin Zhou,Mai-Xin Lu,Miao Wang,Zhi-Gang Liu,Jian-Meng Cao,Xiao-Li Ke,Meng-Meng Yi,Deng-Gao Qiu
Toll-like receptors (TLRs) play a critical role in the innate immune response of fish. In this study, we isolated the cDNA sequence of Nile tilapia TLR1 ( On TLR1). The deduced OnTLR1 protein contains a signal peptide , 7 leucine-rich repeats (LRRs), a C-terminal LRR (LRR-CT), a transmembrane region and a highly conserved TIR domain. In healthy Nile tilapia, the On TLR1 transcript was broadly expressed in all examined tissues, with the highest expression levels in the spleen . After infection with Streptococcus agalactiae , the On TLR1 transcripts were upregulated in the gill and kidney. After stimulation with polyinosinic-polycytidylic acid (poly(I:C)), the expression levels of On TLR1 were significantly downregulated in the intestine , whereas On TLR1 transcripts were significantly upregulated in the kidney. After challenge with lipopolysaccharide (LPS), the expression levels of On TLR1 were significantly upregulated in the spleen and kidney. The subcellular localization showed that On TLR1 was expressed in the cytoplasm. TLR1 significantly increased MyD88-dependent NF-κB activity. However, the results of a pull-down assay showed that On TLR1 did not interact with MyD88 or TIRAP. Binding assays revealed the specificity of OnTLR1 for pathogen-associated molecular patterns (PAMPs) and bacteria that included S. agalactiae , Aeromonas hydrophila and poly(I:C) and LPS. Taken together, these findings suggest that OnTLR1, as a pattern recognition receptor (PRR), might play an important role in the immune response to pathogen invasion.
研究领域:
免疫学
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分子生物学
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鱼类生物学
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病原识别
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Lei Tian,Hualin Jiang,Pinghua Chen,Qi Wang,Pingping Niu,Yangming Shi,Minghui Zhou,Yuancheng Qing,Xvbiao Luo
Competing for adsorption active sites decrease the efficiency of simultaneous adsorption of co-existent adsorbates. On the other hand, different adsorbates being desorbed at the same eluting condition will cause they are mixed together after the desorption, and ruin the recovery. Aim at these problems, a novel adsorbent with two functional domains was designed in this study. This adsorbent is a nano hybrid with two functional domains. One is GO domain which is responsible for the adsorption of inorganic metal ions ; another is PNIPAm domain which is responsible for the adsorption of organic molecules. This two-function-domain structure can avoid organic and inorganic adsorbates to compete the same adsorption active sites. Pb 2+ and 4-NP were taken as the model inorganic and organic adsorbates in this study. The locations of adsorbed Pb 2+ and 4-NP were confirmed by the results of adsorption experiments, BET and XPS analysis. The structure of hybrid was studied by FTIR, Raman spectra and TGA analysis. The hybrid exhibits high adsorption capacity toward Pb 2+ and 4-NP with high anti-inference ability. Furthermore, Pb 2+ and 4-NP can be efficiently desorped one by one with acidic or alkalinous condition, respectively. So they will not be mixed together after the desorption, and can be sequentially recovered. Due to the high efficiency and easy operation, the hybrid is considered as a promising adsorbent to simultaneously adsorb and recover organic and inorganic resources, and the method reported here could provide a new thinking way to develop highly effective adsorbents.
研究领域:
环境科学
·
材料科学
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Xinchen Chen,Hongtao Xia,Jianqi Lv,Yuting Liu,Yang Li,Lejin Xu,Chuting Xie,Yun Wang
Reasonable surface modification and increasing structural porosity were two key factors to improve the adsorption performance of biochar in the adsorption field. In this study, three different proportions of bamboo-based magnetic biochar modified with Fe 3 O 4 (Fe 3 O 4 @MBC) were synthesized by “one-can” method. BET showed that this method could effectively increase the specific surface area (129.79 m 2 g −1 ) and VSM indicated Fe 3 O 4 @MBC had an excellent magnetic response (32.84 emu g −1 ), which was conducive to the separation after adsorption. The accurate matching of pseudo-second-order kinetic model, thermodynamic model and Langmuir isotherm showed that at 298 K, pH = 4.5, the adsorption process dominated by chemical complexation and monolayer adsorption was spontaneous, endothermic and disordered. Saturated adsorption capacity of Fe 3 O 4 @MBC with the more Fe 3 O 4 loading proportion could reach 70.45 mg g −1 within 12 h. The loading of Fe 3 O 4 by “one-can” method not only provided abundant Fe-O groups to assist adsorption, but also magnetized bamboo matrix. Results showed that adsorption mechanism of U(VI) by Fe 3 O 4 @MBC included electrostatic action and chemical complexation involving Fe-O, carbonyl group and ample oxygen-containing functional groups. Therefore, Fe 3 O 4 @MBC with easy separation, excellent regeneration, remarkable economic potential and green value provided a novel solution for the field of radioactive wastewater treatment.
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CATENA
·
2022
·
IF 6.367
Yuhang Jiang,Xiaoqin Lin,Muhammad Umar Khan,Wenjia Jiang,Yina Xu,Zhong Li,Wenxiong Lin
Leaf and branch pruning is essential for improving the productivity of continuously cropped leaf-harvested crops, such as tea, which is conductive to stabilize the production, delay the aging, and subsequently improve economic benefits. Generally, farmers adopt crew-cut pruning to get more yield in tea orchard pruning management. However, the long-term effects of crew-cut pruning on ecosystem of tea orchard are unclear. The present study investigated the environmental differences of ecosystem between crew-cut tea (CT) pruning and umbrella-shaped tea (UT) pruning treatments in the Tieguanyin tea orchards of China. We analyzed the soil enzymes activities, the rhizosphere and phyllosphere's microbial communities, the secondary metabolites of fresh tea shoots, and the insect abundance and its diversity in the two types of tea orchards. The results showed that both saccharase and urease were improved at different soil layers by UT. Fresh tea shoots analysis revealed 22.91% and 16.59% higher epigallocatechin gallate and epicatechin gallate levels, respectively under UT regime than those in CT, but a 9.42% lower ratio of polyphenol to the amino acid in comparision with that in CT. The total volatiles, especially ( E )-geraniol and furanoid linalool oxides, in the fresh shoots of UT were also significantly higher than those in CT. Further analysis showed that UT had more beneficial insects than CT. In contrast, tea gardens in CT were at a higher risk of pest attack than that in UT. Prolonged umbrella pruning of tea plantations significantly improved the bacterial diversity and the beneficial fungi in the leaf surface. Moreover, the relative abundances of rhizobia and ammonia-oxidizing bacteria in the rhizosphere were higher in UT than that in CT. The findings suggest that tea pruning in umbrella-shaped can reduce rhizosphere soil degradation and improve the ecosystem functioning, suggesting that proper pruning pattern is needed to improve tea quality.
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