TOXICOLOGY AND INDUSTRIAL HEALTH
·
2024
·
IF 1.7
Yuxin Wang,Yi Guo,Jiajia Ren,Qiling Liu,Chong Wang
Earlier research has demonstrated that developmental exposure to bisphenol A (BPA) has persistent impacts on both adult brain growth and actions. It has been suggested that BPA might obstruct the methylation coding of the genes in the brain. In this study, the methylation changes in the hippocampus tissue of male rat pups were examined following prenatal BPA exposure. Pregnant Sprague-Dawley rats were treated with either vehicle (tocopherol-stripped corn oil) or BPA (4, 40, or 400 μg/kg·body weight/day) throughout the entire duration of gestation and lactation. At 3 weeks of age, the male rat offspring were euthanized, and the hippocampus were dissected out for analysis. The expression levels of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) and DNA demethylases (TET1, Gadd45a, Gadd45b, and Apobec1) were analyzed in the hippocampus by means of quantitative real-time polymerase chain reaction and Western blotting, respectively. The results showed that prenatal exposure to BPA upregulated the expression of enzymes associated with DNA methylation and demethylation processes in the hippocampus of male rat offspring. These findings suggest that prenatal exposure to a low dose of BPA could potentially disrupt the balance of methylation and demethylation in the hippocampus, thereby perturbing epigenetic modifications. This may represent a neurotoxicity mechanism of BPA.
研究领域:
神经科学
·
表观遗传学
·
毒理学
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DOI
PubMed
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 1
·73-79
·
IF 1.474
Chomba Haji,Pan Ting,Zhuo Xinyue,Zhao Lan,Wang Yulin,Huang Zhao,Martin Haikael,Chen Dongchu,Nie Libo
Simple, cost effective, high sensitive and selective detection strategies for disease related DNA sequences in clinical diagnostics and research purposes are still on demand. Detection of DNA specific sequences of particular biomedical importance, based on electrochemical signaling,
has been reported as a promising analytical approach for medical diagnostics due to its simplicity, cost effective, sensitivity, selectivity and rapidity. Herein, a simple and cost effective DNA biosensor based on poly T30 Copper Nanoparticle Signaling and biobarcode technique is presented
for the first time. In this design, complementary sequence places the poly T30 modified bio-barcode probe (P2-SiO2-T30) on the sensor interface. Upon copper reduction reaction, copper nanoparticles (CuNPs) are clustered along the poly T30 modified bio-barcode probe (P2-SiO2-T30-CuNPs).
During electrochemical measurements copper nanoparticles (CuNPs) are oxidized to give current signal. This detection strategy has a detection limit of 10 pM.
研究领域:
生物医学工程
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分析化学
·
纳米技术
·
医学诊断
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DOI
高影响因子
ANALYST
·
2013
·Vol. 139
·期 4
·801-806
·
IF 3.969
Jing Mei Fang,Fei Leng,Xi Juan Zhao,Xiao Li Hu,Yuan Fang Li
A label-free and sensitive fluorescence method for recognition of sequence-specific DNA using DNA-intercalating dye and metal–organic frameworks (MOFs) is developed. Here, MIL-101 (Cr3F(H2O)2O[(O2C)–C6H4–(CO2)]3·nH2O) is introduced as a quenching platform to decrease the high background fluorescence of SYBR Green I (SG)/probe DNA complex. Mechanism investigations show that MIL-101 can strongly adsorb the SG/probe DNA complex through π–π stacking and electrostatic interactions, and as a consequence, the fluorescence of the SG dye is greatly quenched. While in the presence of target DNA, the as-formed rigid double-stranded (ds) structure of DNA will be far away from the surface of MIL-101; meanwhile, the SG dye can be bound with the dsDNA in the mode of intercalation and minor groove binding, resulting in enhancement of the SG dye fluorescence. The increased signal-to-background ratio has a linear relationship with the concentration of target DNA in the range of 0.1–14 nM. It is confirmed that the detection limit is 73 pM (3σ), which is much lower than that based on the carbon nanotubes and graphene oxide platform. Moreover, one-base-mismatched target DNA can be discriminated effectively. With the introduction of MIL-101, the signal-to-background ratio has been improved ∼8-fold, demonstrating that MIL-101 is an efficient low-background signal platform.
研究领域:
分析化学
·
生物化学
·
纳米技术
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 10
·7765-7771
·
IF 3.289
Ruipeng Hou,Xiaoqing Niu,Fengling Cui
A highly sensitive and selective label-free biosensor was designed for DNA determination based on target-induced opening of the hairpin DNA and forming a G-quadruplex with protoporphyrin IX (PPIX) as a signal reporter. In the absence of target-DNA (T-DNA), the probe-DNA (P-DNA) forms a hairpin structure. A G-rich sequence in the strand is partially caged in the stem-loop structure and cannot fold into a G-quadruplex. In the presence of T-DNA, the specific combination of targets with the sensing sequence (loop sequence) triggers the release of the G-rich sequence and allows it to fold properly and bind with PPIX resulting in the fluorescence of the G-quadruplex–PPIX providing the readout signal for the sensing event (detection limit of 3.5 nM). Based on the higher efficiency of Pb2+ at stabilizing G-quadruplexes than K+ and the Pb2+-stabilized G-quadruplexes not binding to PPIX, which resulted in fluorescence decrease, the proposed DNA-sensing system can be further exploited as a Pb2+-sensing method. The detection limit for Pb2+ is 2.6 nM.
研究领域:
分析化学
·
生物化学
·
环境科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2012
·
IF 5.602
Nai-ying Wu,Wei Gao,Xu-lun He,Zhu Chang,Mao-tian Xu
A direct electrochemical DNA biosensor based on zero current potentiometry was fabricated by immobilization of ssDNA onto gold nanoparticles (AuNPs) coated pencil graphite electrode (PGE). One ssDNA/AuNPs/PGE was connected in series between clips of working and counter electrodes of a potentiostat , and then immersed into the solution together with a reference electrode , establishing a novel DNA biosensor for specific DNA detection. The variation of zero current potential difference (Δ E zcp ) before and after hybridization of the self-assembled probe DNA with the target DNA was used as a signal to characterize and quantify the target DNA sequence . The whole DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of ferricyanide as an electrochemical redox indicator . Under the optimized conditions, Δ E zcp was linear with the concentrations of the complementary target DNA in the range from 10 nM to 1 μM, with a detection limit of 6.9 nM. The DNA biosensor showed a good reproducibility and selectivity . Prepared DNA biosensor is facile and sensitive, and it eliminates the need of using exogenous reagents to monitor the oligonucleotides hybridization.
研究领域:
分析化学
·
电化学
·
生物传感器
·
分子诊断
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-13
·
IF 5.3
Zhao Tangtang,Xiao Ruilin,Li Yueqi,Ren Jierong,Niu Liyun,Chang Bingmei
With their regulated Boolean logic operations in vitro and in vivo, DNA logic circuits have shown great promise for target recognition and disease diagnosis. However, significant obstacles must be overcome to improve their operational efficiency and broaden their range of applications. In this study, we propose an Exo III-powered closed-loop DNA circuit (ECDC) architecture that integrates four highly efficient AND logic gates. The ECDC utilizes Exo III as the sole enzyme-activated actuator, simplifying the circuit design and ensuring optimal performance. Moreover, the use of Exo III enables a self-feedback (autocatalytic) mechanism in the dynamic switching between AND logic gates within this circulating logic circuit. After validating the signal flow and examining the impact of each AND logic gate on the regulation of the circuit, we demonstrate the intelligent determination of miR-21 using the carefully designed ECDC architecture in vitro. The proposed ECDC exhibits a linear detection range for miR-21 from 0 to 300 nM, with a limit of detection (LOD) of approximately 0.01 nM, surpassing most reported methods. It also shows excellent selectivity for miR-21 detection and holds potential for identifying and imaging live cancer cells. This study presents a practical and efficient strategy for monitoring various nucleic acid-based biomarkers in vitro and in vivo through specific sequence modifications, offering significant potential for early cancer diagnosis, bioanalysis, and prognostic clinical applications. Graphical abstract
研究领域:
分子生物学
·
生物医学工程
·
诊断学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 49
·43501-43508
·
IF 3.289
Hongzhi Wang,Yu Wang,Su Liu,Jinghua Yu,Yuna Guo,Ying Xu,Jiadong Huang
In this work, a novel electrochemical DNA sensor based on exonuclease III (Exo III)-assisted autocatalytic DNA biosensing platform for ultrasensitive detection of antibiotics has been reported. When the arched probe was challenged with target, the released primer caused by the specific recognition of the aptamer and target antibiotics hybridized with the 3′-protruding terminus in the HP1. Then, Exo III could catalyze the stepwise removal of mononucleotides from this flat terminus, resulting in the release of the primer and trigger. As a secondary target, trigger could replace Helper from the 5′-MB labeled HP2, and HP2 formed a “close” probe structure, confining MB close to the electrode surface for efficient electron transfer. To our best knowledge, such work is the first report about Exo III-assisted autocatalytic DNA biosensing platform combing with signal-on sensing strategy, which has been utilized for quantitative determination of antibiotics. It would be further used as a general strategy associated with more analytical techniques toward the detection of a wide spectrum of analytes. Thus, it holds great potential for the development of ultrasensitive biosensing platform for the applications in bioanalysis, disease diagnostics, and clinical biomedicine.
研究领域:
生物分析
·
分子诊断
·
临床生物医学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yuwan Du,Shuaifeng Ji,Qingyang Dong,Jiang Wang,Dianpeng Han,Zhixian Gao
Nucleic acid markers have been widely used in the detection of various virus-related diseases, including hepatitis B virus (HBV), which is spreading worldwide. The trans -activated CRISPR-Cas system has shown excellent sensitivity and specificity in nucleic acid detection. However, nucleic acid testing usually requires amplification of the target nucleic acid for more accurate and specific detection; furthermore, current nucleic acid assays are time-consuming, costly, and are limited by non-specific cross-reactivity. We developed an amplification-free viral DNA biosensor-based diagnostic method that uses a clustered regularly interspaced short palindromic repeats-associated system (CRISPR/Cas)-based approach with surface enhanced Raman spectroscopy . This method can specifically identify the target site by changing the crRNA sequence. In addition, the incubation period and development of the disease can be determined by quantitative detection of viral DNA. This system could achieve rapid and highly sensitive detection of HBV DNA within 50 min and vast detection range from 0.1 pM to 1 nM. Therefore, a combined CRISPR/Cas12a-SERS-based assay would improve the sensitivity of detection in assays using multiple biomarkers. In conclusion, our CRISPR/Cas12a-based biosensor would enable rapid, simple, and sensitive detection of HBV nucleic acids.
研究领域:
病毒学
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分子诊断
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Xueping Yang,Jingyun Li,Suixin Zhang,Chao Li,Jiehua Ma
CRISPR/Cas-based systems are highly attractive for developing next-generation diagnostic technologies because of their intrinsic merits such as simplicity, sensitivity, and specificity. However, currently, nucleic acid amplification procedures are still needed to achieve attomolar sensitivity in most CRISPR/Cas-based assays, which causes high cost, operation difficulty, and low efficiency. Herein, we combine the CRISPR/Cas12a-based assay and a single-microbead detection platform for one-step and amplification-free detection of DNA at the single-molecule level. By modifying DNA reporters on a biomimetic membrane-coated microbead, the activated Cas12a by targets will cleave these reporters and lighten the bead within 10 min. The method allows the detection of the target down to three copies in a 5 μL sample. Furthermore, we successfully apply this method for the specific identification of viral infection, foodborne bacteria, and DNA mutation in real samples without extra nucleic acid amplification. We believe that this approach offers new insights for developing CRISPR/Cas-based DNA assays in biomedical applications.
研究领域:
分子生物学
·
生物技术
·
诊断技术
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2012
·
IF 2.098
Shucai Liang,Hui Yu,Jin Xiang,Wang Yang,Xiaohui Chen,Yanbin Liu,Chen Gao,Guoping Yan
A new naphthalimide modified polyethylenimine (PEI) nanoparticles (called NPEI-NPs) was synthesized and applied as fluorescent probe for rapid, selective and sensitive fluorometric detection of trace DNA. The synthesis involved the covalent modification of PEI with 4-butylamino-N-carboxymethyl-1,8-naphthalimide(BACMN) for getting amphiphilic polymer. Then the amphiphilic polymer was self-assembled in water to give the NPEI-NPs. NPEI-NPs was soluble in water and emitted fluorescence at 545 nm with exciting at 460 nm. The fluorescence spectra resulting from the interaction between NPEI-NPs and DNA indicated that the fluorescence of NPEI-NPs increased in the present of DNA. Therefore, a fluorescence enhancement method was developed for the determination of trace fish sperm DNA (fsDNA) and calf thymus DNA (ctDNA). Under the optimal conditions, the calibration curves were linear over the concentration ranges of 0.05–2.8 μg/mL for fsDNA and 0.08–3.0 μg/mL for ctDNA. The detection limits for fsDNA and ctDNA were 1.6 and 2.0 ng/mL, respectively. The proposed method has been employed to quantify DNA in synthetic samples with the satisfactory results.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 9
·4044-4052
·
IF 6.97
Lijun Geng,Xudong Yu,Yajuan Li,Yanqiu Wang,Yongquan Wu,Jujie Ren,Fengfeng Xue,Tao Yi
Biomolecule-based hydrogels have potential use in a wide range of applications such as controlled drug release, tissue engineering, and biofabrication. Herein, driven by specific interactions between ds-DNA (double-stranded DNA) and Zn2+ based metal-complexes, we report that the use of DNA as cross-linkers can enhance interactions between self-assembling Zn2+ complexes containing terpyridine and sugar groups in the generation of bioinspired hydrogels from solutions or suspensions. The gelation process is fast and straightforward without tedious steps and happens at room temperature. Such a hydrogelation process of different Zn2+ complexes endows the visualized and selective DNA analogue discrimination. Several experiments suggest that the strong intercalation binding of Zn2+ complexes with ds-DNA results in the unzipping of ds-DNA into ss-DNA (single-stranded DNA), which further behave as linkers to enhance the intermolecular interactions of self-assembling Zn2+ complex molecules via coordination interactions. This work demonstrates an efficient and universal strategy to prepare hydrogels based on biomolecular recognition. Moreover, the DNA responsive behaviors of Zn2+ complexes are further compared with that of solutions and cells.
研究领域:
材料科学
·
生物技术
·
生物化学
·
纳米技术
·
药学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 19
·5842-5847
·
IF 4.019
Yifan Fei,Chenxiao Yan,Yali Yu,Longlong Gao,Ting Ye,Qingqing Zhang,Heng Gao,Xiaoshun Zhou,Yong Shao
Depurination occurs via hydrolysis of the purine-deoxyribose glycosyl bond and causes nucleic acid damage. In particular, the DNA sequences that can undergo a self-catalyzed depurination (SCD) will cause a great uncertainty in duplicating, separating, purifying, and storing the DNA samples. Therefore, there is a great demand to develop a rapid detection method for SCD events. Herein, the use of a convenient fluorescence method to follow the site-specific SCD was demonstrated. We found that the resultant apurine site (AP site) from depurination can be selectively recognized by a fluorescent probe of palmatine (PAL) with a turn-on fluorescence response. The dependence of SCD on the bases of the depurination site, pH, metal ions, and time shows that our method can be used to rapidly evaluate the depurination process. Furthermore, the depurination process can be photo-switched using a photoacid as an external initiator. Our work will find wide applications in preliminarily identifying the DNA depurination.
研究领域:
生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
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