BMC Complementary and Alternative Medicine
·
2013
·Vol. 13
·期 1
·1-11
·
IF 2.082
Wang Shan-Shan,Wang Dong-Mei,Pu Wen-Jun,Li Deng-Wu
Background Extracts from Potentilla species have been applied in traditional medicine and exhibit antioxidant, hypoglycemic, anti-inflammatory, antitumor and anti-ulcerogenic properties, but little has been known about the diversity of phytochemistry and pharmacology on this genus. This study investigated and compared the phytochemical profiles, antioxidant and antimicrobial activities of leaf extracts from three Potentilla species (Potentilla fruticosa, Potentilla glabra and Potentilla parvifolia) in order to discover new resources for lead structures and pharmaceutical products.Methods Chemical composition and content of six phenolic compounds were evaluated and determined by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau colourimetric method and sodium borohydride/chloranil-based method (SBC); Antioxidant activities were determined using DPPH, ABTS and FRAP assays; Antimicrobial properties were investigated by agar dilution and mycelial growth rate method.Results The results showed hyperoside was the predominant phenolic compound in three Potentilla species by RP-HPLC assay, with the content of 8.86 (P. fruticosa), 2.56 (P. glabra) and 2.68 mg/g (P. parvifolia), respectively. The highest content of total identified phenolic compounds (hyperoside, (+)-catechin, caffeic acid, ferulic acid, rutin and ellagic acid) was observed in P. parvifolia (14.17 mg/g), follow by P. fruticosa (10.01 mg/g) and P. glabra (7.01 mg/g). P. fruticosa possessed the highest content of total phenolic (84.93 ± 0.50 mmol gallic acid equivalent/100 g) and total flavonoid (84.14 ± 0.03 mmol quercetin equivalent/100 g), which were in good correlation with its significant DPPHIC50 (16.87 μg/mL), ABTS (2763.48 μmol Trolox equivalent/g) and FRAP (1398.70 μmol Trolox equivalent/g) capacities. Furthermore, the effective methodology to distinguish the different species of Potentilla was also established by chromatographic fingerprint analysis for the first time. The results of antimicrobial activities showed P. fruticosa exhibited the strongest inhibition aganist Gram-positive bacteria, Pseudomonas aeruginosa and Candida albicans with MIC values of 0.78–6.25 mg/mL. P. parvifolia possessed antibacterial and antifungal activities against all the microorganisms tested, with EC50 and MIC values of 20.52–47.02 mg/mL and 0.78–50 mg/mL, respectively.Conclusion sThese results indicated that leaf extracts from three Potentilla species could become useful supplement for pharmaceutical products as a new antioxidant and antimicrobial agents.
研究领域:
药用植物学
·
植物化学
·
药学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 3
·1472-1481
·
IF 6.317
Jiaxuan Li,Yu Li,Xuedi Zhang,Song Miao,Mingqian Tan,Wentao Su
Fruit juice is one of the most popular drinks, which requires strict processing conditions to ensure its quality, especially to prevent enzymatic browning and turbidity loss. In this work, a new strategy for the preparation of composite nanofibers for juice clarification and anti-browning control was proposed. The strategy used microfluidic spinning to combine Fucoxanthin (Fx), hydroxypropyl-γ-cyclodextrin (HP-γ-CD) and polyvinyl pyrrolidone (PVP) to prepare Fx/HP-γ-CD-PVP (PCF) nanofibers, which not only reflected the excellent antioxidant properties of cyclodextrin-wrapped Fx, but also achieved a more optimized juice clarification agent dosage. Molecular docking technique was used to prove that the stable inclusion complex of Fx and HP-γ-CD could be formed by hydrogen bonding when the molar ratio of Fx to HP-γ-CD was 1 : 2, and the binding energy was as low as −10.23 kcal mol−1. SEM, XRD, FT-IR and TGA were used to characterize the structure of the composite nanofibers, which showed that the thermal stability and water solubility of the embedded Fx were improved. Further studies showed that the apple juice with PCF nanofibers containing inclusion complexes of Fx and HP-γ-CD at a molar ratio of 1 : 2 (PCF 1 : 2) could significantly improve the DPPH and ABTS radical scavenging activity, and could significantly protect the cell membrane integrity of RAW264.7 cells against H2O2 oxidative damage. Finally, the effects of PCF nanofibers on the quality of fresh juice were studied, including clarification experiment and sensory evaluation. The results showed that the dosage of PVP in PCF 1 : 2 was only about 4% of the conventional dosage, and the browning index of fresh juice was significantly reduced with the best clarification. The available data provided in this study would provide a promising safety strategy for the food processing of fresh juice and the extension of its storage life.
研究领域:
食品科学
·
纳米技术
·
生物技术
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2023
·
IF 3.4
Yang Liu,Limei Ran,Yahong Wang,Peng Wan,Hongli Zhou
The polysaccharides from Sea buckthorn leaves (SBLPs) were extracted by hot water and purified by DEAE cellulose , then separated into six polysaccharides (SBLP-S) by DEAE-52 column. Six separated polysaccharides were characterized by Ultraviolet Spectroscopy, Infrared Spectrum, High Performance Liquid Chromatographic and Congo red analysis. The antioxidant activity and immunological activity were investigated in vitro . The results revealed that the monosaccharide composition of SBLP-S-1, SBLP-S-2, SBLP-S-3, SBLP-S-5 and SBLP-S-6 contained Man , GlcN , Rib , Rha , GluA , GalA , Glu, Gal, Xyl , Ara and Fuc , among them, rare glucosamine was found. And SBLP-S-4 contained all above components except GlcN and GluA. FT-IR showed that SBLP-S were sulfated polysaccharide containing uronic acid . Molecular weights of SBLP-S were 338.659, 401.305, 599.849, 393.904, 626.895 and 176.862 kDa. The Congo-red test indicated that SBLP-S-2, SBLP-S-4, SBLP-S-5, and SBLP-S-6 had triple helix conformation. Crude polysaccharides had the strong scavenging activities on DPPH radicals, ABTS radicals and hydroxyl radicals . The six polysaccharides had the activity of immune stimulation on RAW264.7 cell. SBLP-S-2 promoted the phagocytosis best and SBLP-S-6 promoted the NO production best. The results suggested that SBLPs could be used as potential antioxidants and immunomodulatory agents in pharmaceutical and functional food fields.
研究领域:
药理学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2014
·
IF 3.096
Huiling Kong,Jixin Yang,Yifeng Zhang,Yapeng Fang,Katsuyoshi Nishinari,Glyn O. Phillips
Selenium nanoparticles (SeNPs) were prepared by using gum arabic (GA) as the stabilizer in a facile synthetic approach. The size, morphology, stability and antioxidant activity in vitro of the gum arabic-selenium nanocomposites (GA-SeNPs) were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and ultraviolet–visible spectrophotometry (UV–vis). SeNPs (particle size of ∼34.9 nm) can be stabilized in gum arabic aqueous solutions for approximately 30 days. FTIR results show that SeNPs were combined to the hydroxyl groups of GA. In the present work, the alkali-hydrolyzed GA (AHGA) was also prepared and its efficiency in stabilizing SeNPs was compared with GA. It was concluded that the branched structure of GA was a significant factor for the functionality. The hydroxyl radical scavenging ability and DPPH scavenging ability of GA-SeNPs were higher than those of AHGA-SeNPs and could reach 85.3 ± 2.6%, 85.3 ± 1.9% at a concentration of 4 mg/ml, respectively.
研究领域:
材料科学
·
纳米技术
·
化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2014
·
IF 2.939
Ping Xiao,Hongzhi Huang,Jianwei Chen,Xiang Li
Ethnopharmacological relevance Radix Isatidis called “Ban-Lan-Gen” is one of the most commonly-used traditional Chinese medicines for antiviral, anti-inflammatory, antioxidant and antipyretic purposes. Investigate the bioaccessibility of uridine , epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol as well as the antioxidant and anti-inflammatory activities during an in vitro gastro-intestinal digestion of the Radix Isatidis extract (RIE). Materials and methods High performance liquid chromatography (HPLC) technique was adopted to determine the bioaccessibility of six bioactive compounds in RIE. Antioxidant activities of RIE in different digestive stages were determined by 1,1-Diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydroxyl radical scavenging abilities. Anti-inflammatory activity was assayed by the inhibitions of inflammatory cytokines such as nitrous oxide (NO), prostaglandin E 2 (PGE 2 ) and tumor necrosis factor α(TNF-α) producted by lipopolysaccharide (LPS) stimulated RAW264.7 cells. Results The bioaccessibility of uridine, epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol were 15.38%, 18.28%, 24.01%, 6.50%, 8.65% and 17.78%, respectively. Also, the digestion products still possessed certain antioxidant activities. The antioxidant activity was highly correlated with lignans (clemastanin B, indigoticoside A and isolariciresino). The anti-inflammation activity of the three samples decreased in the order: IN sample (the solution that had diffused into the dialysis tubing)>Nondigested sample (RIE solution)>Gastric sample (post-gastric digestion)>OUT sample (material that remained in the gastro-intestinal tract). Conclusions Results obtained in this research reveal the amount of bioactive compounds from RIE that could be available for absorption in vivo . The antioxidant activity decreased significantly but the anti-inflammatory activity was enhanced in serum-available fraction after gastro-intestinal digestion in vitro . This study could provide a scientific basis for a deeper pharmacological activity study of Radix Isatidis and a simple method for pharmacodynamic material basis research.
研究领域:
药理学
·
民族药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Yaoyao Qi,Leyi Xie,Zeyuan Deng,Bing Zhang,Hongyan Li
The stability and antioxidant activity changes of 10 phytochemicals (isoflavones: genistein, genistin, daidzein, daidzin, formononetin, and anthocyanidins: delphinidin, cyanidin, pelargonidin, malvidin, petunidin) and food representatives (soybeans and blueberries) during in vitro digestion were investigated. Results indicated that the stability of isoflavones and anthocyanidins decreased with digestion. Among the isoflavones, the loss rate of formononetin was the largest, reaching 98.24%, and that of daidzein was the smallest (22.78%). And after intestinal digestion, anthocyanidins degraded into phenolic acids and other compounds, and the loss rate was above 95%. The loss of phenolic compounds in food extract was smaller than the standards, and the loss rate of phenolic compounds in hydrolyzed blueberry extracts (90.66%–96.04%) was higher than that of soybean extract (31.22%–59.74%). Moreover, the antioxidant activity of isoflavones and anthocyanidins decreased after the oral phase. During the gastrointestinal phase, the ABTS, DPPH and FRAP of isoflavones increased, while the ORAC and CAA decreased. The antioxidant activity of anthocyanidins increased in the gastric digestion and decreased in the intestinal phase. The glucoside linkage in the A ring and the hydroxyl methoxylation in the B ring weakened the antioxidant activity of isoflavones, while the hydroxylation and methoxylation of B rings of anthocyanidins could enhance thier antioxidant activity. The changes in antioxidant activity of the two food extracts were consistent with their contents.
研究领域:
食品科学
·
营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Guangkai Wu,Zihan Zhao,Jun Hu,Yue Li,Jianxia Sun,Weibin Bai
The chemical synthesis of anthocyanins, especially delphinidin-3-O-glucoside and petunidin-3-O-glucoside, is preferable due to the challenges associated with their extraction and purification. However, the reported methods for the synthesis are scarce and intricate. Our research focused on exploring a one-step ester-to-ketone process and optimizing the ring formation reaction, simplifying and improving the overall synthesis strategy. Through these attempts, we were able to achieve higher production yields of delphinidin-3-O-glucoside and petunidin-3-O-glucoside. According to the results of DPPH, ABTS, and FRAP, the antioxidant activity of anthocyanins was increased with the number of B ring hydroxyl substituent. Additionally, both delphinidin-3-O-glucoside and petunidin-3-O-glucoside exhibited no cytotoxicity effects, highlighting their potential for safe application in various fields.
研究领域:
化学
·
药学
·
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xiaoli Zhou,Yumeng Qin,Yanli Wang,Yaping Wang,Zhicheng Qin
Rosa roxburghii Tratt (RRT) is renowned for its abundance of phenolics, various methods have been used to extract free phenolics from RRT, there remains a paucity of research on the comparative distinctions between bound phenolic compounds and their free counterparts in RRT, especially in vitro and in vivo antioxidant potential of RRT-BP necessitates further exploration. This study sought to optimize the process parameters for extracting bound polyphenols through alkaline hydrolysis . Among the bound phenolic compounds, 20 phenolic acids and 34 flavonoids were identified, while the free phenolic compounds comprised 19 phenolic acids and 42 flavonoids. The IC 50 values for DPPH and ABTS radical scavenging were 44.92 μg/mL and 60.92 μg/mL for free phenols , 87.02 μg/mL and 95.02 μg/mL for bound phenols, respectively. Compared with the control group, the lifespan of Caenorhabditis elegans fed with 1 mg/mL of free and bound phenol extended from 11.63 d to 16.77 d and 13.88 d, respectively, with no adverse impact on the reproductive capability of the nematodes. The longevity of C. elegans was observed to persist even under thermal and oxidative stress , primarily attributed to elevated superoxide dismutase and catalase activity, coupled with reduced malondialdehyde content in C. elegans . These findings underscore that free polyphenols exhibit superior polyphenolic components and antioxidant properties compared to their bound counterparts. Nevertheless, the potential health effects of bound polyphenols as a supplement to free polyphenols in RRT should not be ignored.
研究领域:
药理学
·
生物化学
·
分子生物学
·
营养学
·
生物技术
✓ 已验证
DOI
高影响因子
Food Quality and Safety
·
2024
·
IF 3
Zhang Rongbin,Zhou Zhilei,Ji Zhongwei,Ren Qingxi,Liu Shuangping,Xu Yuezheng,Mao Jian
Lees were the major by-product of Huangjiu brewing, and prone to decompose due to abundant protein residues. To convert the lees into high-value peptides with umami and antioxidant difunctional, herein, a one-step hydrolysis method combined with flavourzyme followed by amylase pretreatment was developed and optimized. The target peptide fraction was collected and evaluated after ultrafiltration and ethanol precipitation, then identified by nano-HPLC-MS/MS. The results show that 80U/g amylase and 3500U/g flavourzyme synergistic hydrolysis 50℃ for 3h performed best umami intensity and antioxidant activity. A total of 5266 peptides was identified from the 80% ethanol precipitation fraction, then filtered 7 umami peptides through virtual screening, sensory, and radical scavenging verification. The umami recognition threshold of 7 peptides ranged from 0.38 to 0.66 mmol/L at water. Among them, DPDGW and DNPNW exhibited well DPPH antioxidant ability with IC50 values of 0.6982 and 0.4315 mg/mL, respectively. Additionally, molecular docking studies indicated that all umami peptides tend to interact with the T1R3 receptor through hydrogen bonds and van der Waals forces, involving key residues such as ASN68, SER104, HIS145, SER276, VAL277, GLU301, ALA302, THR305, and HIS387. This research provides a reference for the flavor peptide resource development, oriented towards dual functionality in flavor and health.
研究领域:
食品科学
·
生物化学
·
肽化学
·
风味化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2011
·
IF 2.502
Jianbo Xiao,Jianglei Huo,Huixian Jiang,Fan Yang
The chemical compositions and bioactivities of crude tea polysaccharides (TPS) from the out-of-date tea leaves (beyond their useful date), namely Xihu Longjing (XTPS), Anxi Tieguanyin (TTPS), Chawentianxia (CTPS) and Huizhoulvcha (HTPS), in market were investigated. These TPS showed similar neutral sugar content and different distribution of molecular weight (1–800 kD). These crude TPS were mainly composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, and galacturonic acid. IR spectra confirmed that these crude TPS were composed of polysaccharide, protein and uronic acids. These TPS showed similar DPPH scavenging activity and exhibited lower DPPH scavenging activities than Vc within 25–200 μg/mL. However, these TPS with higher concentrations (200–400 μg/mL) showed similar DPPH scavenging activity with Vc. HTPS exhibited significant higher superoxide anion scavenging activity than others TPS and gallic acid. XTPS showed significant higher inhibitory effects on α-glucosidase and α-amylase with inhibitory percentages of 64.35% and 82.24% than others TPS. TTPS, XTPS, and HTPS exhibited similar inhibition ability on α- d -glucosidase and α-amylase. The overdue tea leaves can be a resource of tea polysaccharides as function food.
研究领域:
食品科学
·
营养学
·
药理学
·
农业化学
✓ 已验证
DOI
PubMed
高影响因子
CyTA-Journal of Food
·
2013
·
IF 0.5
Xin-Huai Zhao,Yu Fu,Nan Yue
Casein hydrolysate of a degree of hydrolysis of 9.4% was generated from caseinate using papain and modified by plastein reaction with extrinsic phenylalanine or tyrosine addition, and then fractionated by an ethanol-water solvent (6:4, v/v). Compared to casein hydrolysate or mixtures of casein hydrolysate and phenylalanine or tyrosine, two modified hydrolysates prepared thereof showed higher scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) or hydroxyl radicals and reducing power. The obtained soluble and precipitated fractions had higher and lower total hydrophobic amino acids especially stronger and weaker three antioxidant properties than the parent substrates, respectively. All hydrolysates particularly soluble fractions protected rat hepatocytes against H2O2- or galactosamine-induced injury in vitro, resulting in enhanced cell viability, decreased cellular malondialdehyde content, and lactate dehydrogenase leakage. Cytoprotection of the hydrolysates was observed at a dosage-dependence manner. A negative correlation (P < 0.05) was found between malondialdehyde content and scavenging activity on hydroxyl radicals or reducing power of the hydrolysates.
研究领域:
食品科学
·
生物化学
·
药理学
·
细胞生物学
·
药物化学
✓ 已验证
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2019
·Vol. 13
·期 4
·2790-2797
·
IF 1.415
Han Yi-Tong,Zhao Chen-Chen,Han Jia-Run,Yan Jia-Nan,Du Yi-Nan,Shang Wen-Hui,Wu Hai-Tao
The purpose of the present study was to explore the antioxidant activity of organic extracts from Maillard reaction products (MRPs) of sea cucumber ( Stichopus japonicus ) gut hydrolysates (SCGHs). The SCGHs were obtained after treatment with neutrase, and they were then reacted with ribose at a mass ratio of 1:2, pH 8.0, and 95 °C for up to 12 h. The MRPs were extracted by methanol and ethanol. The methanol extracts of the MRPs had a higher UV absorbance, browning intensity, and fluorescence intensity than the crude MRPs. The scavenging capacities of methanol and ethanol extracts from MRPs in both 2′,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assays increased significantly ( p < 0.05). The methanol extracts were better than the ethanol extracts showing the reduced IC 50 levels for ABTS and DPPH by 50% and 50%, respectively. The inhibitory activity for peroxidation of linoleic acid of MRPs was also enhanced in the methanol extracts of MRPs, but this was reduced in ethanol extracts. Moreover, HepG2 cell viability after exposure to the methanol extracts of MRPs was increased to 59.30 ± 0.43% compared with the SCGHs and MRPs, which was 3.97 times that of the H 2 O 2 -treated group. These results suggest that methanol extraction can improve the antioxidant activity of SCGHs-ribose MRPs and that the corresponding extracts can be potentially used as food antioxidants.
研究领域:
食品科学
·
营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）