RSC Advances
·
2018
·Vol. 8
·期 60
·34428-34436
·
IF 2.936
Sihui Zhan,Qianlei Hou,Yi Li,Shuanglong Ma,Pengfei Wang,Yanan Li,Haitao Wang
Visible-light-driven photocatalytic disinfection is highly desired for water treatment due to its advantages such as wide applicability and being free of disinfection byproducts. In this study, AgBr/g-C3N4 hybrid nanocomposites were evaluated as photocatalysts under visible light irradiation for water disinfection using Escherichia coli as a model pathogen. The physicochemical and photo-electrochemical properties of the photocatalyst were systematically characterized using advanced techniques including scanning electron microscopy (SEM), transmission electron microscopy (HRTEM), powder X-ray diffraction (XRD), UV-visible diffuse reflectance spectra (DRS), X-ray photoelectron spectroscopy (XPS), photoluminescence (PL) spectra and electron spin resonance (ESR) spectroscopy. The inactivation mechanism of E. coli was systematically investigated by monitoring the morphology change of the bacteria and analyzing the role of reactive species. The optimized AgBr/g-C3N4 hybrid photocatalyst exhibited remarkably enhanced visible-light-driven photocatalytic disinfection performance towards E. coli over that of pure g-C3N4 and AgBr under visible light, which could completely inactivate 107 cfu mL−1 E. coli in 90 min. Quenching studies indicated that h+ is the main reactive species responsible for inactivating E. coli. The mechanism study revealed a Z-scheme charge transfer mechanism between AgBr and g-C3N4. The g-C3N4 could effectively trap the photogenerated conduction band electrons of AgBr via a Z-scheme type of route, thus significantly promoting the electron–hole separation. The trapping of electrons by g-C3N4 could facilitate h+ accumulation, which accounts for the better disinfection performance of AgBr/g-C3N4 compared to AgBr and g-C3N4.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Jinbin Liu,Jing Jiang,Yajun Bai,Tai-ping Fan,Ye Zhao,Xiaohui Zheng,Yujie Cai
Bacteria rarely produce natural 2-phenylethanol. We verified a new pathway from Proteus mirabilis JN458 to produce 2-phenylethanol using Escherichia coli to coexpress l-amino acid deaminase, α-keto acid decarboxylase, and alcohol dehydrogenase from P. mirabilis. Based on this pathway, a glucose dehydrogenase coenzyme regeneration system was constructed. The optimal conditions of biotransformation by the recombinant strain E-pAEAKaG were at 40 °C and pH 7.0. Finally, the recombinant strain E-pAEAKaG produced 3.21 ± 0.10 g/L 2-phenylethanol in M9 medium containing 10 g/L l-phenylalanine after a 16 h transformation. Furthermore, when the concentration of l-phenylalanine was 4 g/L (24 mM), the production of 2-phenylethanol reached 2.88 ± 0.18 g/L and displayed a higher conversion rate of 97.38 mol %.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2025
·
IF 2.8
Zhou Caixue,Dai Shuxia,Yin Li,Zhuo Yu,Zhao Nannan,Li Dehai,Sun Changyan
Purpose In this paper, we use Pinus pumila (Pall.) Pinecone to extract essential oil and improve its utilization. Methods The study used hydrodistillation to extract essential oil from Pinus pumila (Pall.) pinecone. Inhibition analysis of E. coli using extracted essential oils was evaluated to reveal the inhibitory activity by assessing growth curves, conductivity, nucleic acids, proteins, enzyme activities, and protein synthesis. Results The primary constituents, as shown by GC-MS chemical composition study, were α-pinitol, β-pinene and limonene. The results of the bacteriostatic activity indicated that the inhibitory zone (DIZ) had a diameter of 14.93 ± 0.14 mm, the minimum bactericidal concentration (MBC) was 16.0 µL/mL, and the minimum inhibitory concentration (MIC) was 8.0 µL/mL. Sweeping electron microscopy and laser confocal also demonstrated the disruption of cell wall and cellular enzymes, analyzed by CATase and ATPase activities, which showed a decrease in enzyme activity, and SDS-PAGE analysis showed its ability to inhibit protein synthesis, which leads to cellular damage. Conclusion The present study emphasizes the sustainable use of Pinus pumila (Pall.) Pinecone for economic opening of food preservation in addition to inhibition of spoilage bacteria.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Qiulan Li,Jitao Li,Yang Jiao,Xiaolan Yang,Dezhi Yang,Zitao Zhong,Yaling Yang
The pathogenic bacteria induced foodborne disease has been detrimental to public health worldwide. Herein, the peroxidase (POD)-like Fe 3 O 4 /MWCNTs@Mo-CDs (FMMC) nanozyme was applied for the detection of Escherichia coli ( E. coli ). The E. coli aptamer was conjugated with the surface of the FMMC, which effectively enhanced the POD-like activity attributing to the higher affinity to the substrate, and then specific capture of E. coli in food matrices, leading to the reduction of POD-like activity. Therefore, a robust and facile colorimetric aptasensor was developed for detecting E. coli with a wide linear range of 10 1 –10 6 CFU/mL, low LOQ of 10 1 CFU/mL and LOD of 0.978 CFU/mL. The aptasensor demonstrated the satisfied selectivity for E. coli compared to the other strains. This method possessed the potential application for fast in situ screening of foodborne pathogens in food products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DAIRY SCIENCE
·
2016
·
IF 2.408
Shan Shan,Daofeng Liu,Qi Guo,Songsong Wu,Rui Chen,Kai Luo,Liming Hu,Yonghua Xiong,Weihua Lai
In this study, cascade signal amplification in ELISA involving double-antibody sandwich ELISA and indirectly competitive ELISA was established to sensitively detect Escherichia coli O157:H7. In the double-antibody sandwich ELISA, a complex was formed comprising anti- E. coli O157:H7 polyclonal antibody, E. coli O157:H7, biotinylated anti- E. coli O157:H7 monoclonal antibody, streptavidin, and biotinylated β-lactamase. Penicillin solution was then added into the ELISA well and hydrolyzed by β-lactamase. Afterward, the penicillin solution was transferred to indirectly competitive ELISA. The concentration of penicillin can be sensitively detected in indirectly competitive ELISA. In the cascade signal amplification system, increasing the amount of added E. coli O157:H7 resulted in more β-lactamase and less penicillin. The detection sensitivity of E. coli O157:H7, which was 20 cfu/mL with the cascade signal amplification in ELISA, was 1,000-fold higher than that of traditional ELISA. Furthermore, the novel method can be used to detect E. coli O157:H7 in milk (2 cfu/g). Therefore, this new signaling strategy will facilitate analyses of highly sensitive foodborne pathogens.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Song Lin,Zhengyu Tao,Zhenhui Li,Shangsong Li,Xiaoliang Wang,Xiaoman Liu,Xin Huang
The development of biotic-abiotic photosynthetic systems is becoming one of the most promising strategies towards the thirsty demand for sustainable energy production. However, considerable efforts are still required to further optimize the design and avoid the utilization of high-value substances while achieving highly efficient hydrogen production. Here we show a way to construct an Escherichia coli ( E. coli )-quantum dots hybrid system by expressing ferredoxin (Fd), Fd-NAD(P) + reductase (FNR), and [FeFe]-hydrogenase ([FeFe]H 2 ase) as a potential biocatalyst. We reveal that electrons originating from the quantum dots could be redirected through the newly established electron transport chain NADH/NAD + -FNR-Fd to [FeFe]H 2 ase, which contributes a key mechanism to boost the highest H 2 production activity (3149.5 μmol g dcw −1 h −1 ) by only using glycerol as the carbon source. Our work sheds light on the advantages of an intracellular biological hybrid system by combining the photocatalytic capacity with the optimized metabolic power to produce biofuels.
✓ 已验证
产品关联
DOI
高影响因子
POULTRY SCIENCE
·
2025
·
IF 4.2
Mengru Chen,Yifei He,Yimin Jia,Lei Wu,Ruqian Zhao
Avian pathogenic Escherichia coli ( APEC ) infection has high morbidity and mortality, and multiple stressors encountered during rearing place poultry in a state of stress. However, research on how poultry cope with APEC infection under stress situation is still limited. In this study, we established a broiler stress model by corticosterone ( CORT ) administration subcutaneously for 7 consecutive days, followed by APEC challenge intramuscularly. CORT treatment significantly reduced body weight ( BW ) and average daily gain ( ADG ) while increasing feed conversion ratio ( FCR ) ( P < 0.01). APEC infection significantly decreased ADG ( P < 0.01). CORT treatment and APEC infection elevated plasma corticosterone and heterophil to lymphocyte ( H/L ) ratio ( P < 0.05). Additionally, plasma aspartate aminotransferase ( AST ), AST to alanine aminotransferase ( AST/ALT ) ratio, and lactate dehydrogenase ( LDH ) levels increased significantly ( P < 0.01). Histopathological analysis revealed structural damage of liver, indicating that CORT treatment and APEC infection induced liver injury. However, CORT pretreatment broilers exhibited a milder histopathological lesions and significantly lower AST, ALT, and LDH levels ( P < 0.05) compared to APEC infection alone. CORT treatment and APEC infection increased plasma levels of lysozyme ( LZM ), total protein ( TP ), and globulin ( GLOB ) ( P < 0.05), while CORT pretreatment further elevating their concentrations compared to APEC infection alone, suggesting an enhanced innate immune response. Liver transcriptomic analysis identified 768, 335, and 567 differentially expressed genes ( DEGs ) following CORT, APEC, or both treatments, respectively, enriched in cytokine-cytokine receptor interaction, PPAR signaling pathway, Toll-like receptor signaling pathway, MAPK signaling pathway, steroid hormone biosynthesis pathway, arachidonic acid metabolism, and phagosome pathway, etc., indicating that CORT treatment regulates lipid metabolism and immunity, while APEC infection induces inflammation and disrupts lipid metabolism. Notably, CORT pretreatment may mitigate APEC induced liver injury by enhancing phagosome function. Moreover, glucocorticoid receptor ( GR ) may regulate DEGs expressions, thus affected broilers response to CORT, APEC, or both treatments. These results suggest that CORT treatment, APEC infection, or both significantly affect the growth performance, immune response and liver function of broilers, while lipid metabolism may play a crucial role.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2011
·
IF 5.361
Yingqiao Teng,Xinai Zhang,Ying Fu,Huijie Liu,Zhongchuan Wang,Litong Jin,Wen Zhang
A novel amplified electrochemical immunoassay based on ferrocene (Fc)-functionalized ZnO nanorods (NRs) was developed in the present work. The detection antibody ( d Ab) and Fc were immobilized onto the surface of ZnO NRs, denoted as { d Ab–ZnO–Fc} bioconjugates. The amount of d Ab and Fc in the bioconjugates was investigated using the copper reduction/bicinchoninic acid reaction (BCA protein assay) and inductive coupled plasma-atomic emission spectroscopy (ICP-AES), respectively. Greatly amplified signal was achieved in the sandwich-type immunoassay when d Ab and Fc linked to ZnO NRs at a proper ratio. Using Escherichia coli ( E. coli ) as a model antigen, the designed immunoassay showed an excellent analytical performance, and exhibited a wide dynamic response range of E. coli concentration from 10 2 to 10 6 cfu/mL with a detection limit of 50 cfu/mL ( S / N = 3). By introducing a pre-enrichment step, the detection of 5 cfu/10 mL E. coli in hospital sewage water was realized. This proposed signal amplification strategy was promising and could be easily extended to monitor other biorecognition events.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Zhengfeng Guo,Tingting Zhang,Hao Yang,Xiaolin Zhu,Siyuan Lu,Anjie Chen,Mingyu Fan,Jiao Qu
In aquatic environments, antibiotics degrade into byproducts, potentially enhancing bacterial resistance. However, the specific mechanisms by which these byproducts induce bacterial resistance remain elusive. This study conducted experimental evolution experiments to explore how E. coli adapts to tetracycline (TC) and its primary degradation products—anhydrotetracycline (ATC), epitetracycline (ETC), and 4-epianhydrotetracycline (EATC)—through evolution experiments. Prolonged exposure to TC and its byproducts significantly increased frequency of resistant mutants in E. coli ATCC25922, with a maximum 10 6 -fold increase. Resistant mutants exhibited markedly elevated minimum inhibitory concentrations (MICs) for TC, ampicillin (AMP), and ciprofloxacin (CIP), indicating multidrug resistance. Transcriptomic analysis showed that the antibiotic resistance phenotype could be related to enhanced target protection, metabolic adaptations, and reduced membrane permeability. The induction pathways between TC and its byproducts were distinct. Specifically, TC 20d (where TC 20d represents the mutants collected after 20 days of continuous exposure to TC) was associated with more alterations in ribosome-associated genes, which was correlated with an enhanced defensive response as shown by the data. Moreover, variations in energy metabolism gene expression suggest a robust metabolic defense in ATC 20d and ETC 20d . When TC and its byproducts—ATC, ETC, and EATC—act together, they induce antibiotic resistant mutants at rates of 29.8 %, 18.9 %, 18.3 %, and 31.9 %, respectively. This study provides a descriptive overview of the possible adaptive mechanisms and pathways that may be involved in antibiotic resistance due to environmental exposure.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2025
·Vol. 14
·期 8
·914
·
IF 6.6
Pengxin Jiang,Kangping Liu,Yanan Cui,Puyu Liu,Xutao Wang,Zijuan Hou,Jiamei Cui,Ning Chen,Jinghui Fan,Jianguo Li,Yuzhu Zuo,Yan Li
EnterotoxigenicEscherichia coli(ETEC) leads to severe diarrhea in piglets. Naringenin (Nar), a natural flavonoid compound, is known for its antibacterial and anti-antioxidant properties. However, the protective effects of Nar against ETEC-induced diarrhea have not been reported yet. This study investigated the protective mechanisms of Nar against ETEC infection in porcine intestinal epithelial cells (IPEC-J2). ETEC infection induced oxidative stress and ferroptosis in IPEC-J2 cells by elevating intracellular iron content and ROS accumulation, increasing MDA levels, downregulating SOD activity and GPX4 expression, and upregulating the transcription of CHAC1 and SLC7A11. In contrast, Nar suppressed ETEC-induced ferroptosis of IPEC-J2 cells by inhibiting the SLC7A11/GPX4 pathway. Specifically, Nar mitigated mitochondrial damage, reduced intracellular iron levels and ROS accumulation, and ultimately reversed the oxidative stress. Network pharmacology and molecular docking identified heat-shock protein 90 (HSP90) as a potential target of Nar. Overexpression and knockdown experiments revealed that ETEC-induced ferroptosis was mediated by upregulation of HSP90, while the protective effects of Nar against ETEC-induced ferroptosis were dependent on the downregulation of HSP90. In conclusion, Nar targets host HSP90 to protect IPEC-J2 cells from ferroptosis caused by ETEC infection. This study demonstrates that Nar is a potent antioxidant natural compound with potential for preventing ETEC-induced intestinal damage.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yanying Rao,Hong Li,Xiaoying Ding,Binggui Wang,Yuanli Liu,Xiaoxu Zhao
In this study, Surface-enhanced Raman Spectroscopy (SERS) and Fourier Transform Infrared Spectroscopy (FTIR) were employed to investigate the molecular changes in Escherichia coli ( E. coli ) induced by exposure to ampicillin (AMP), enrofloxacin (ENR), ciprofloxacin (CIP), and norfloxacin (NFX) over time. The optimal concentration of E. coli for SERS analysis was determined to be 50 μL of bacterial suspension, diluted six times to achieve an OD600 ≈ 0.1. The primary changes in the SERS spectra were observed at 1267 cm −1 , corresponding to the amide III band in proteins, while the FTIR spectra revealed significant changes in the 1200–900 cm −1 range, associated with carbohydrates, under AMP treatment. ENR, CIP, and NFX, which are quinolone antibiotics, act as inhibitors of DNA synthesis. The main changes in the SERS spectra for antibiotic-resistant E. coli were observed at 760 cm −1 (attributed to cytosine and uracil), 960 cm −1 (C N stretching and C C deformation), and 1140 cm −1 (C O C stretching and ring breathing). In the FTIR spectra, significant changes were detected at 1655 cm −1 , 1544 cm −1 , and 1239 cm −1 , corresponding to the amide I, amide II, and amide III bands, respectively. The combination of SERS and FTIR with principal component analysis (PCA) enabled the detection of molecular modifications in antibiotic-resistant E. coli exposed to different classes of antibiotics. These findings enhance our understanding of the mechanisms of action of antibiotics in bacteria.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2024
·
IF 3.7
Chi Zhang,Guoguang Wei,Ning Zhou,Yingying Wang,Jia Feng,Xin Wang,Alei Zhang,Kequan Chen
In this study, we proposed a biological approach to efficiently produce pseudouridine (Ψ) from glucose and uracil in vivo using engineered Escherichia coli. By screening host strains and core enzymes, E. coli MG1655 overexpressing Ψ monophosphate (ΨMP) glycosidase and ΨMP phosphatase was obtained, which displayed the highest Ψ concentration. Then, optimization of the RBS sequences, enhancement of ribose 5-phosphate supply in the cells, and overexpression of the membrane transport protein UraA were investigated. Finally, fed-batch fermentation of Ψ in a 5 L fermentor can reach 27.5 g/L with a yield of 89.2 mol % toward uracil and 25.6 mol % toward glucose within 48 h, both of which are the highest to date. In addition, the Ψ product with a high purity of 99.8% can be purified from the fermentation broth after crystallization. This work provides an efficient and environmentally friendly protocol for allowing for the possibility of Ψ bioproduction on an industrial scale.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）