Frontiers in Chemistry
·
2021
·
IF 5.221
Chen Kaiwei,Du Linlin,Gao Peng,Zheng Junli,Liu Yuanli,Lin Hua
The carboxylate-functionalized passion fruit peel (PFPCS) was an efficient and rapid biosorbent for wastewater treatment. The PFPCS exhibited excellent selectivity to the cationic dyes, where the maximum adsorption capacities for methylene blue (MB) and methyl violet (MV) were 1,775.76 mg g−1 and 3,756.33 mg g−1, respectively. And the adsorption process of MB and MV on PFPCS reached equilibrium within 20 min. Moreover, the adsorption conditions and mechanisms were investigated. The adsorption process was in good agreement with the pseudo-second-order and Langmuir isotherm models. The adsorption mechanism was also proposed to be electrostatic interaction and hydrogen bond. After six cycles of desorption-adsorption, the removal efficient of MB and MV could be kept above 95%. Thus, PFPCS was considered as a highly efficient absorbent for removing cationic dyes from polluted water due to excellent adsorption characteristics, low cost and environmental friendliness.
研究领域:
环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Li Wang,Timin Shan,Bing Xie,Chen Ling,Shuang Shao,Peng Jin,Yonghua Zheng
Glycine betaine (GB) treatment is useful to reduce chilling injury (CI) of several kinds of fruits including peach. However, the regulatory mechanism remains unknown. In this study, peach fruit was treated with 10 mmol L −1 GB solution for 10 min. The effects of GB treatment on CI, phenolic and soluble sugar metabolism were investigated in this study. Moreover, phenylpropanoid and soluble sugar content, and enzyme activities associated with phenolic and sugar metabolisms were also measured. The results showed that GB reduced CI and maintained high levels of total phenolic and flavonoid content. The activities of phenylpropanoid metabolism-related enzymes were significantly enhanced by GB. Higher content of sucrose and lower contents of fructose and glucose were observed in GB-treated fruits. Therefore, our results showed that GB could enhance chilling tolerance of peach through regulating phenolic and sugar metabolisms, and maintaining high levels of individual phenolic and sucrose content.
研究领域:
植物生理学
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食品科学
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农业化学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2019
·
IF 2.87
Kai Tian,Jiang Zhu,Mei Li,Xinghui Qiu
Capsaicin (8-methyl- N -vanillyl-6-nonenamide) is the most abundant capsaicinoids found in hot peppers ( Capsicum annum and Capsicum frutescens ). It has been well documented that capsaicin plays an important role in the defense against the attack of herbivores or pathogens on Capsicum plants. A few insect herbivores such as Helicoverpa armigera and Helicoverpa assulta have been recorded to be capable of feeding on hot pepper fruits, suggesting that these insects evolve mechanisms against the toxicity of capsaicin. Although cytochrome P450-meidated detoxification is considered to be an important mechanism by which cotton bollworms cope with capsaicin, experimental evidence is lacking. In this study, we compared the capacity of four H. armigera P450s (CYP6B6, CYP9A12, CYP9A14 and CYP9A17) in capsaicin metabolism, and the capsaicin metabolites were screened and tentatively identified by liquid chromatography–tandem mass spectrometry (LC–MS/MS). HPLC analyses showed that depletion rates of capsaicin were 21.9 ± 0.1, 11.9 ± 1.5, 16.3 ± 1.4 and 14.8 ± 0.2 min −1 for CYP6B6, CYP9A12, CYP9A14 and CYP9A17 respectively. The transformation of capsaicin was inhibited by the P450 inhibitor piperonyl butoxide . A total of seven products were detected, and hydroxylation (aromatic and aliphatic) and dehydrogenation were found to be two main pathways in capsaicin metabolism. In addition, capsaicin metabolism was enzyme selective: M1 (ω-hydroxylated N-macrocyclic metabolite) and M3 (ω-hydroxylated metabolite) were uniquely detected in the CYP6B6 catalytic reaction, while M4 (ω-n hydroxylated capsaicin), M5 (diene of capsaicin) and M6 (doubly oxidized metabolite of dehydrogenated capsaicin) were only detectable in CYP9A metabolisms. A capsaicin dimer (5, 5′-dicapsaicin) was found to be the major metabolite of CYP9A reactions, but the minor product produced by CYP6B6. An overall more similar behavior in capsaicin metabolism was observed among CYP9As than between CYP6B6 and CYP9As. Our data demonstrate that CYP6B6 and CYP9As have a potent capability to transform capsaicin, and individual P450 produce unique metabolite profile. These findings help us to understand the molecular basis of capsaicin adaptation in H. armigera .
研究领域:
昆虫学
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植物化学
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分子生物学
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DOI
PubMed
高影响因子
PLANTA
·
2015
·Vol. 243
·期 1
·183-197
·
IF 3.263
Chen Jingxin,Mao Linchun,Lu Wenjing,Ying Tiejin,Luo Zisheng
Main conclusion
Auxin and abscisic acid regulate strawberry fruit ripening and senescence through cross-talk of their signal transduction pathways that further modulate the structural genes related to physico-chemical properties of fruit.
The physiological and transcriptomic changes in harvested strawberry fruits in responses to IAA, ABA and their combination were analyzed. Exogenous IAA delayed the ripening process of strawberries after harvest while ABA promoted the postharvest ripening. However, treatment with a combination of IAA and ABA did not slow down nor accelerate the postharvest ripening in the strawberry fruits. At the molecular level, exogenous IAA up regulated the expressions of genes related to IAA signaling, including AUX/IAA , ARF , TOPLESS and genes encoding E3 ubiquitin protein ligase and annexin, and down regulated genes related to pectin depolymerization, cell wall degradation, sucrose and anthocyanin biosyntheses. In contrast, exogenous ABA induced genes related to fruit softening, and genes involved in signaling pathways including SKP1 , HSPs , CK2 , and SRG1 . Comparison of transcriptomes in responses to individual treatments with IAA or ABA or the combination revealed that there were cooperative and antagonistic actions between IAA and ABA in fruit. However, 17 % of the differentially expressed unigenes in response to the combination of IAA and ABA were unique and were not found in those unigenes responding to either IAA or ABA alone. The analyses also found that receptor-like kinases and ubiquitin ligases responded to both IAA and ABA, which seemed to play a pivotal role in both hormones’ signaling pathways and thus might be the cross-talk points of both hormones.
研究领域:
植物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
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IF 4.219
Lishan Xu,Yaojie Zhang,Lizhi Wang
Dragon fruit is a tropical fruit with good taste. It can bring health benefits to human body. As one of the major bioactive components in this fruit, the polysaccharides might contribute to the health benefits. However, the precise structure information remains unknown. A leading polysaccharide of dragon fruit pulp, DFPP, was purified and identified by NMR and GC–MS. →4-β- d -Glc p A-1→, →6-β- d -Gal p -1→ and →4-α- l -Rha p -1→ constituted the backbone and α- l -Ara f -1 → 5-α- l -Ara f -1→ formed the branch chain. The precise structure was putatively identified as below. The molecular weight was 2.2 × 10 3 kDa. The structure information of polysaccharides will be helpful to understand this fruit. Download : Download high-res image (88KB) Download : Download full-size image
研究领域:
食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Tang Liqin,Liu Haocheng,Wen Jing,Xu Yujuan,Tian Wenni,Li Lu,Yu Yuanshan,Lin Xian,Fu Manqin
The acquisition of yellow extract (YE) from gardenia ( Gardenia jasminoides ) fruit can promote the application of natural pigment. This study aims to establish an effective method for extracting YE from gardenia fruit and to evaluate the properties of YE. First, the seven compounds in YE obtained by ultrahigh-pressure extraction (UPE) were identified by LC-TOF-MS/MS. The effects of traditional extraction (TE), ultrasonic extraction (UE), and UPE methods on the yield of YE were then investigated. The results showed that the extraction yield of UPE was 7.73 ± 0.89 mg/g DW under the optimized conditions (47.78% ethanol concentration, 114.95 s time, and 18.08 mL/g liquid–solid ratio), which was higher than those of UE (4.81 ± 0.23 mg/g DW) and TE (3.55 ± 0.21 mg/g DW). Finally, the YE stability of UPE was studied. The results showed that the YE stability of UPE was similar to that of the TE group, and both of which were greatly affected by higher temperature (60–100 °C), ultraviolet radiation, alkalinity , and Fe 3+ . Meanwhile, YE of UPE had better stability under ultraviolet radiation and alkalinity in comparison with the TE group. Therefore, UPE is a potential method for YE extraction from gardenia fruit.
研究领域:
食品科学
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植物化学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Bi Wang,Pirui Li,Jingjing Yang,Xuhong Yong,Min Yin,Yu Chen,Xu Feng,Qizhi Wang
Phytophthora capsici is a notorious pathogen capable of infecting various host plants and causing serious threats to agriculture worldwide. Tetradium glabrifolium fruit essential oil (TFO) is widely utilized in traditional Chinese medicine treatment, but its antimicrobial activity is unknown. In this study, we first investigated the inhibitory efficacy of TFO against P. capsici and found TFO strongly inhibited the development of phytophthora blight on pepper by in vivo inoculation assay. Second, we found TFO could significantly suppress vegetative and reproductive growth of P. capsici in vitro . Microscope observations indicated that TFO destroyed the mycelial morphology and cellular ultrastructure of P. capsici. Additionally, TFO could damage the cell membrane integrity of the pathogen, leading to cellular leakages of intercellular contents of P. capsici . Third, TFO could enhance the activities of defence-related enzymes in pepper fruits during pathogen infection. Finally, we identified the chemical composition of TFO by GC-MS analysis and demonstrated three abundant components ( D -limonene, β -elemene, and 2-tridecanone) contributed to the antifungal activity of TFO. Together, out results revealed that TFO had outstanding inhibitory efficacy against P. capsici , and uncovered its potential antifungal mechanism. This study would propose the application of TFO as an efficient alternative approach for controlling phytophthora blight.
研究领域:
植物学
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植物病理学
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农业科学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Huan Cheng,Wenyan Wu,Jin Chen,Haibo Pan,Enbo Xu,Shiguo Chen,Xingqian Ye,Jianle Chen
Black wolfberries ( Lycium ruthenicum Murray) establish various biological activities, owing to anthocyanins, polysaccharides, pectin and so on. However, minor appearance differences are found in black wolfberries from different geographical origins. Thus, in this study, total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC) were determined in black wolfberries from four provinces in China (Xinjiang, Qinghai, Gansu, Ningxia Provinces) for obtaining a distinguishable chemical index. Compared with TPC and TFC, TAC largely was affected by environment conditions, and thus, it may be a good chemical marker for geographical origin identification. Then, anthocyanin profiles were analysed by ultra-performance liquid chromatography (UPLC) coupled with triple quadrupole (triple Q)-time-of-flight mass spectrometry (TOF-MS), and principal component analysis (PCA) was applied for geographical origin identification based on anthocyanin fingerprints. A total of 17 anthocyanins were tentatively determined including two new individual anthocyanins, delphinidin-3-rutinoside ( cis - p -coumaroyl)-5-O-diglucoside and delphinidin-3-rutinoside ( trans - p -coumaroyl)-5-O-diglucoside. PCA indicated that individual anthocyanin contents calculated according to HPLC data and raw HPLC data both allowed a clear separation of geographical origins of black wolfberry fruits, explaining more than 95% of information of the samples. The present study could provide a scientific guidance for distinguishing the black wolfberry from different geographical origins.
研究领域:
食品科学
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分析化学
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植物化学
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地理标志
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产品关联
DOI
高影响因子
Food & Function
·
2022
·Vol. 13
·期 3
·1472-1481
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IF 6.317
Jiaxuan Li,Yu Li,Xuedi Zhang,Song Miao,Mingqian Tan,Wentao Su
Fruit juice is one of the most popular drinks, which requires strict processing conditions to ensure its quality, especially to prevent enzymatic browning and turbidity loss. In this work, a new strategy for the preparation of composite nanofibers for juice clarification and anti-browning control was proposed. The strategy used microfluidic spinning to combine Fucoxanthin (Fx), hydroxypropyl-γ-cyclodextrin (HP-γ-CD) and polyvinyl pyrrolidone (PVP) to prepare Fx/HP-γ-CD-PVP (PCF) nanofibers, which not only reflected the excellent antioxidant properties of cyclodextrin-wrapped Fx, but also achieved a more optimized juice clarification agent dosage. Molecular docking technique was used to prove that the stable inclusion complex of Fx and HP-γ-CD could be formed by hydrogen bonding when the molar ratio of Fx to HP-γ-CD was 1 : 2, and the binding energy was as low as −10.23 kcal mol−1. SEM, XRD, FT-IR and TGA were used to characterize the structure of the composite nanofibers, which showed that the thermal stability and water solubility of the embedded Fx were improved. Further studies showed that the apple juice with PCF nanofibers containing inclusion complexes of Fx and HP-γ-CD at a molar ratio of 1 : 2 (PCF 1 : 2) could significantly improve the DPPH and ABTS radical scavenging activity, and could significantly protect the cell membrane integrity of RAW264.7 cells against H2O2 oxidative damage. Finally, the effects of PCF nanofibers on the quality of fresh juice were studied, including clarification experiment and sensory evaluation. The results showed that the dosage of PVP in PCF 1 : 2 was only about 4% of the conventional dosage, and the browning index of fresh juice was significantly reduced with the best clarification. The available data provided in this study would provide a promising safety strategy for the food processing of fresh juice and the extension of its storage life.
研究领域:
食品科学
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纳米技术
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生物技术
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化学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2020
·Vol. 44
·期 3
·e13141
·
IF 1.662
Canying Li,Junhu Zhang,Yonghong Ge,Xiaoyan Li,Meilin Wei,Jiabao Hou,Yuan Cheng,Jingyi Lv
“Golden delicious” apples were dipped in 100 mg/L acibenzolar-S-methyl (ASM) to investigate the fruit quality and softening during 12 days of storage. Weight loss, flesh firmness, ethylene release, respiratory rate, content of total soluble solids and titratable acid, the activity of pectinase, cellulase, and β-glucosidase, and water-insoluble pectin and water-soluble pectin contents were investigated. The results demonstrated that ASM treatment inhibited ethylene release and respiratory rate, reduced titratable acidity, and enhanced total soluble solids content in apples. Moreover, application of ASM suppressed the reduction of flesh firmness, activity of pectin methylesterase, and polygalacturonase. Cellulase, β-glucosidase, and degradation of protopectin in apple fruit were also suppressed by ASM treatment during storage. In conclusion, ASM could maintain fruit quality by regulating cell wall-degrading enzymes during storage. Practical applications Application of acibenzolar-S-methyl after harvest has the potential of delaying fruit softening by regulating cell wall-degrading enzymes, thus retain fruit quality.
研究领域:
农业
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食品科学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2020
·Vol. 20
·期 1
·1-17
·
IF 3.497
Xue Cheng,Guan Si-Cong,Chen Jian-Qing,Wen Chen-Jin,Cai Jian-Fa,Chen Xu
Background Pectin methylesterase (PME) is a hydrolytic enzyme that catalyzes the demethylesterification of homogalacturonans and controls pectin reconstruction, being essential in regulation of cell wall modification. During fruit ripening stage, PME-mediated cell wall remodeling is an important process to determine fruit firmness and softening. Strawberry fruit is a soft fruit with a short postharvest life, due to a rapid loss of firm texture. Hence, preharvest improvement of strawberry fruit rigidity is a prerequisite for extension of fruit refreshing time. Although PME has been well characterized in model plants, knowledge regarding the functionality and evolutionary property of PME gene family in strawberry remain limited.Results A total of 54 PME genes (FvPMEs) were identified in woodland strawberry (Fragaria vesca ‘Hawaii 4’). Phylogeny and gene structure analysis divided these FvPME genes into four groups (Group 1–4). Duplicate events analysis suggested that tandem and dispersed duplications effectively contributed to the expansion of the PME family in strawberry. Through transcriptome analysis, we identified FvPME38 and FvPME39 as the most abundant-expressed PMEs at fruit ripening stages, and they were positively regulated by abscisic acid. Genetic manipulation of FvPME38 and FvPME39 by overexpression and RNAi-silencing significantly influences the fruit firmness, pectin content and cell wall structure, indicating a requirement of PME for strawberry fruit softening.Conclusion Our study globally analyzed strawberry pectin methylesterases by the approaches of phylogenetics, evolutionary prediction and genetic analysis. We verified the essential role of FvPME38 and FvPME39 in regulation of strawberry fruit softening process, which provided a guide for improving strawberry fruit firmness by modifying PME level.
研究领域:
植物生物学
·
基因组学
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分子遗传学
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农学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhuo Sun,Juan Lu,Xin Zhang,Xiangyu Shan,Qian Wu,Chao Li,Huiling Li,Shuning Yang,Li Tian
In this work, Zn-MOF electrospun nanofibres (Zn-MOF NFs), CdTe and Au@Pt-rGo were applied to Ru(bpy) 3 2+ /tri- n -propylamine electrochemiluminescence (ECL) system. And a novel ECL sensor for the determination of fenpropathrin was constructed for the first time. Due to the large surface area and pores, Zn-MOF NFs can both carry CdTe and Au@Pt-rGo and promote luminescence by facilitating electron transfer and reducing electron transport paths. CdTe participated in the luminescence reaction and improved the system’s sensitivity together with Ru(bpy) 3 2+ . Au@Pt-rGo can promote electron transport in the system. Under the synergistic effect of the three, the ECL sensor had achieved satisfactory results in weak alkaline solution. The constructed ECL sensor can detect fenpropathrin in the range of 4 × 10 −11 –4 × 10 −6 M, and the detection limit was 1.33 × 10 −11 M (S/N = 3). It can be used to detect fenpropathrin in juice samples, which provided a new idea for determining fenpropathrin.
研究领域:
分析化学
·
环境科学
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食品安全
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）