JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Jiangxiong Zhu,Hao Yin,Yidong Xin,Lumei Wang,Xueqing Geng,Haiyun Zhao,Yun Deng
A novel structure-switching “turn-on” fluorometric aptasensor was developed for simultaneous detection of Hg(II) and Pb(II) in grapes, broccoli, and cabbage. The sensing mechanism utilizes fluorescence resonance energy transfer (FRET) between a FAM-labeled aptamer (FAM-Apt) and a BHQ1-labeled complementary strand (CS-BHQ1). In the absence of target ions, FAM-Apt and CS- BHQ1 bind through complementary base pairing, bringing FAM and BHQ1 into close proximity, which induces FRET and quenches FAM’s fluorescence. Target ion binding induces a conformational change in the FAM-Apt, disrupting FRET and restoring fluorescence. Optimized parameters include reagent addition sequence, Apt and CS concentrations, incubation times, buffer conditions, and salt concentrations. Under optimized conditions, the aptasensor achieved limits of detection (LODs) of 2.29 μg/L for Hg(II) and 0.24 μg/L for Pb(II), much lower than the regulatory limits for fruits and vegetables, and the limits of quantification (LOQ) for food samples were 6.02 μg/kg and 2.07 μg/kg, respectively. Furthermore, the constructed aptasensor could rapidly complete detection within 22.5 min and demonstrated high selectivity against interfering ions. Spiked sample recoveries ranged from 98.07-104.12% for Hg(II) and 89.23-105.46% for Pb(II), with relative standard deviations of 0.95-5.64% and 1.21-14.53%, respectively. This aptasensor provides a sensitive, rapid, and cost-effective alternative to conventional detection methods.
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高影响因子
RSC Advances
·
2025
·Vol. 15
·期 45
·38069-38081
·
IF 4.6
Mingmin Bai,Wenyuan Guo,Yiyang Zhang,Ruiqiang Yang,Weixin Li
A novel multifunctional carbon-modified MoS2 material (C-W-D-MoS2-x) was synthesized through a solvothermal method, exhibiting expanded interlayer spacing, a large surface area (∼47.03 m2 g−1), and abundant exposed sulfur (S) active sites that enabled efficient adsorption of Hg2+ from wastewater. Among the prepared variants, C-W-D-MoS2-0.03 showed the highest adsorption performance, achieving an exceptional distribution coefficient (Kd) of 2.0 × 105 mL g−1. The adsorption kinetics were best described by the pseudo-second-order model, while the adsorption isotherms were well-fitted to the Langmuir model, with a maximum adsorption capacity (qm) of 1974.0 mg g−1. This remarkable adsorption capability of C-W-D-MoS2-0.03 can be attributed to the synergistic effect of carbon functional groups and the high density of S active sites. Furthermore, an alumina inorganic membrane functionalized with C-W-D-MoS2-0.03 was successfully assembled into a device, demonstrating a dynamic removal process that reduced 50 mg L−1 of Hg2+ to below 0.1 mg L−1.
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高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2024
·
IF 3.4
Wu Shihao,Zhao Peng,Wang Aihua,Dong Linpei,Wu Xiaojun,Wang Jifen,Zhang Yunfeng
Monochromatic excitation X-ray fluorescence (ME-XRF) spectrometry emerges as a novel technique in trace element analysis, distinguished by its simplicity, rapidity, and efficiency. Its application in forensic toxicology has grown significantly for identifying toxic trace metals in biological samples. This study further expands its utility by developing a method for the rapid determination of arsenic (As), mercury (Hg), thallium (Tl), and lead (Pb) in hair samples. Calibration curves were established using analytical reference samples, and optimal detection conditions were determined and validated. The LODs for As, Hg, Tl, and Pb were 0.03, 0.03, 0.03, and 0.05 μg g −1 , respectively. Method precision was evaluated through seven parallel analyses of samples within the concentration range of 0.27 to 20.051 μg g⁻ 1 , with relative standard deviations (RSDs) consistently below 10%. The feasibility of the ME-XRF technique for forensic toxicology analysis was validated through the analysis of authentic poisoned hair samples collected from the animal experiment.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wanyan Wu,Yan Guo,Shunyu Hu,Hui Li,Jiangpeng Tang,Jingwen Ling,Jiaqiong Xiong,Jiaming Ou,Chang-ye Hui
Mercury (Hg) is a toxic heavy metal that primarily accumulates and biotransforms in water bodies. Though accurate, instrumental detection methods often have high thresholds, making them unsuitable for frequent monitoring. This study introduces a novel whole-cell biosensor that utilizes the IdgS-Sfp system to biosynthesize indigoidine, a water-soluble blue pigment. This approach eliminates the need for extraction methods typically used for lipophilic pigments. The biosensor employs genetically engineered E. coli strains equipped with optimized genetic circuits. Key enhancements include incorporating the MerC transport protein and optimizing induction temperature and time to improve sensitivity and expand the detection range. The IdgS-Sfp-based biosensor exhibits high sensitivity with a limit of detection of 0.08 nM and a broad quantitative range of 2.44–156.25 nM. It shows high selectivity for Hg(II) even in the presence of multiple metal ions and maintains robust performance across various environmental water matrices, including freshwater and seawater. This biosensor offers a promising tool for rapid and cost-effective Hg(II) detection in environmental samples with potential environmental monitoring applications.
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Inorganic Chemistry Frontiers
·
2024
·
IF 6.1
Feng Xu,Xiang Xu,Bingxuan Li,Ge Zhang,Chan Zheng,Jindong Chen,Ning Ye
Long-wave infrared (IR) nonlinear optical (NLO) crystals hold great promise for extensive applications. However, IR NLO crystals with outstanding comprehensive optical and NLO performances are still in great demand. Herein, two Hg-based chalcogenides, Hg3AsS4Cl and Hg3AsS4Br, are synthesized via a chemical vapor transport technique. Attributed to the synergistic combination of [HgS3] groups and structurally aligned [AsS3] groups with lone pair electrons, the two compounds exhibit superior NLO performance, encompassing strong second-harmonic generation (SHG) effects, tremendous birefringence, and a broad transparency range, positioning them as promising long-wave IR NLO materials. Moreover, the luminescent performances of the two compounds are also investigated, revealing interesting ultraviolet (UV) region luminescence emissions, broad emission bands, and large Stokes shifts for potential luminescent applications.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 3
·1-8
·
IF 5.705
He Liuying,Lu Yuexiang,Wang Feiyang,Gao Xinxin,Chen Ying,Liu Yueying
The authors describe a colorimetric method for the determination of Hg 2+ ions based on the inhibition of the activity of the enzyme urease. The pH value of solution increases when urease hydrolyzes urea, which can be visualized by adding a pH indicator such as Phenol Red (PhR). Mercaptoethanol as a typical thiol is added to the system to improve selectivity because it binds metal ions and then - unlike the Hg 2+ mercaptoethanol complex - does not inhibit urease. Hence, the color of the pH indicator PhR turns from yellow to pink as the solution becomes alkaline. The Hg 2+ mercaptoethanol complex, in contrast, strongly inhibits urease and the color of the solution remains yellow. The findings were used to design a photometric assay based on the measurement of the ratio of absorptions of PhR at 558 nm and 430 nm. It has a linear response over the 25 to 40 nM Hg 2+ concentration range and a 5 nM detection limit. This is well below the guideline values of Hg 2+ specified by the US Environmental Protection Agency and the World Health Organization for drinking water (10 nM and 30 nM, respectively). The method was employed to the determination of Hg 2+ in water samples spiked with 10 nM levels of Hg 2+ where color changes still can be observed visually.
Schematic presentation of a colorimetric method for the ultrasensitive detection of Hg 2+ based on the inhibition of urease activity. Mercaptoethanol is used to improve the selectivity. Even at Hg 2+ concentrations as low as 5 nM, the color change still can be easily observed by bare eyes.
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高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2021
·Vol. 68
·期 9
·1739-1747
·
IF 1.967
Xiaoqing Wang,Jing Zhang,Zimeng Xu,Chen Rao,Leilei Pi,Yaqin Fu,Yubing Dong,Chen Shen,Lanying Yao,Chunhua Xiong
The MCTS-g-AT was developed as a novel magnetic adsorbent with core-shell structure. Its unique synthesis route endows MCTS-g-AT with green, convenient, economical and safe characteristics, and also good selective adsorption of Hg(II). The material was characterized using an appropriate set of spectroscopic and analytical techniques (SEM, EDS, XRD, VSM, FTIR, and TGA). Its adsorption kinetics fits the pseudo-secondary model well and adsorption isotherm fits the Langmuir equation well, suggesting the mechanism of chemisorption with monolayer adsorption. The results of the thermodynamic parameters indicate spontaneous and endothermic process. Column adsorption experiments showed that the maximum adsorption capacity of Hg(II) was 207.6 mg/g (298 K). Moreover, the Hg(II) adsorbed on composite microspheres was completely eluted by 3 mol/L HNO 3 or 4 mol/L HCl. Furthermore, MCTS-g-AT adsorption column combined with spectrophotometer can achieve more accurate detection of Hg(II) in aquatic products.
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JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Hongyu Guo,Linfang Liang,Jiayi Guo,Liangbin Lin,Fafu Yang
The metal-coordination self-assemble system played an important role on constructing novel supramolecular smart-response materials. Although the single and double-response materials based on metal-induced self-assembly had been studied extensively, the triple responses for this kind of supramolecular material was seldom reported. In this work, the first metal-induced self-assemble system with triple responses on photophysical, liquid crystalline and gel properties was presented based on Hg 2+ -coordination tetraphenylene dimer ( Bis-TPE ). Bis-TPE was prepared by bridging two alkyl-substituted tetraphenylene with amido chain. It possessed the fluorescence response from the strong emission for Bis-TPE to the faint emission for Bis-TPE -Hg 2+ complex. The self-assemble mechanism of Bis-TPE -Hg 2+ was clarified as that Hg 2+ was located in the cavity of the amido-ether chain of Bis-TPE . The Hg 2+ -induced mesomorphic transition was also observed from smectic mesophase for Bis-TPE to hexagonal columnar mesophase for Bis-TPE -Hg 2+ complex. The unique Hg 2+ -induced gel transition was further distinguished from low-order spherical morphology for Bis-TPE to high-order fibrous morphology for Bis-TPE -Hg 2+ complex. The maximum fluorescence wavelength exhibited the giant shift from 494 nm for Bis-TPE in gel to 606 nm for Bis-TPE -Hg 2+ in gel. This kind of triple responses on photophysical, liquid crystalline and gel properties for metal-coordination self-assemble system was described for the first time, affording a new idea on design of new multiple-response materials with unique properties.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Zhu Yu,Zaiwen Tian,Zhengqian Li,Zaoli Luo,Yan Li,Yi Li,Jun Ren
In this paper, a thioacetal chemosensor 1 incorporating simultaneously a carbazole moiety and an azobenzene moiety was synthesized. Among other metal ions , Hg 2+ proved to be able to selectively induce both color and fluorescence changes of 1 . The mechanism of Hg 2+ -promoted cleavage of thioacetal was confirmed by 1 H NMR experiments. Upon the addition of Hg 2+ into the yellow CH 3 CN/H 2 O solution of 1 , the resultant azobenzene-containing thioglycolamide was precipitated from the solution as a mercuric salt, leading to a colorless solution. Therefore, a novel color change pattern was developed.
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高影响因子
Frontiers in Plant Science
·
2020
·
IF 4.402
Ji Chao,Liu Zhaoyang,Hao Liping,Song Xin,Wang Changdong,Liu Yue,Li Huying,Li Chaohui,Gao Qixiong,Liu Xunli
The present study investigated the physiological and biochemical characteristics of Enterobacter cloacae HG-1 isolated from saline-alkali soil. We further studied the effect of this strain on the salt tolerance of wheat and on the community structure of nitrogen-fixing bacteria in rhizosphere soil. We determined that the investigated strain had high nitrogen fixation activity and produced iron carriers, 1-aminocyclopropane-1-carboxylic acid deaminase, and plant hormones. The metabolites of this strain contained 2,3-butanediol, [R-(R*, R*)], 2-heptanone, and other growth-promoting and antibacterial substances. The strain was also highly salt-tolerant (10% NaCl). After the inoculation of wheat with the HG-1 strain, we recorded increases in root length, plant height, fresh weight, and dry weight of 19.15%, 18.83%, 16.67%, and 17.96%, respectively, compared with uninoculated plants (P < 0.05). Compared with the leaves of uninoculated plants, the proline concentration in the leaves of inoculated plants increased by 12.43% (P < 0.05), the malondialdehyde level decreased by 27.26% (P < 0.05), K+ increased by 20.69%, Ca2+ increased by 57.53% and Na+ decreased by 31.43% (all P<0.05). Furthermore, we detected that inoculation with the HG-1 strain did not affect the species composition of nitrogen-fixing bacteria in wheat rhizosphere soil at the phylum level. However, the average relative abundance of Proteobacteria was significantly increased, whereas the abundance of Verrucomiorobia was significantly decreased compared with uninoculated plants. At the genus level, we detected 32 genera in control samples and 27 genera in inoculated samples, and the species diversity and relative abundance of samples inoculated with the HG-1 strain decreased compared with uninoculated plants. Inoculated samples had lower abundances of Azospirillum, Rhodomicrobium, and Anabaena. Our study demonstrated that the inoculation of wheat with E. cloacae HG-1 could promote the growth of wheat under salt stress and increase salt stress tolerance. The results of this study investigating the interaction among soil, plants, and microorganisms supplement agricultural microbial databases and could provide a reference for the development of microbial-based saline soil improvement programs.
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高影响因子
CHEMICAL PAPERS
·
2022
·Vol. 76
·期 11
·7037-7049
·
IF 2.146
Zhang Lin,Jiao Xinyue,Zhang Huijuan,He Suyan,Cheng Xinjian
Heavy metal ions are harmful to the environment and the human beings and other creatures. Fluorescent agents play important roles in the detection of heavy metals. Chitosan-based fluorescent probes were synthesized and investigated in this work. Firstly, 4-bromo-1,8-naphthimide was introduced into chitosan through nucleophilic addition–elimination reaction, and then the Br was replaced by amino acid through substitution reaction. By this way, three chitosan–naphthalimide–amino acid fluorescent probes were obtained consequently. The structure and fluorescence properties of macromolecular probes were characterized and confirmed. These macromolecules have good selectivity and sensitivity to Hg 2+ /Hg + , and Fe 2+ ions. When Hg + /Hg 2+ ions are present, the probe P1 produces a significant increase in fluorescence intensity. The LOD of P1 to Hg + and Hg 2+ are 1.66 × 10 −6 M and 1.60 × 10 −6 M, respectively. When Fe 2+ ions exist with the presence of P2 and P3 probes, P2 and P3 show significant fluorescence quenching, and their LODs are 1.09 × 10 −6 M and 6.65 × 10 −7 M, respectively. And these three chitosan derivatives also have good adsorption effect on ions, the maximum adsorption capacity of mercury can reach 108.7 mg/g, and the maximum adsorption capacity of ferrous iron can reach 52.0 mg/g. This mechanism of recognition and adsorption is formed as the complexation of N and ions on the probe. In this article, a material with good recognition, and removal of Hg + /Hg 2+ and Fe 2+ is developed, which also provides a way for the potential remediation of heavy metal polluted water.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Wenge Yang,Shouhai Yang,Qirun Guo,Tuan Zhang,Keyi Wu,Yonghong Hu
A phenothiazine–aminothiourea-based open chemosensor ( P1 ) has been designed and synthesized, which exhibits high selectivity toward Hg 2+ by forming a 1:1 complex. In its Hg 2+ ensemble, it is reported as a highly sensitive and selective probe for the detection of iodide based on the displacement approach in aqueous solution. The addition of iodide to a solution of phenothiazine–aminothiourea–Hg 2+ ensemble resulted in a rapid and remarkable fluorescence off–on (emission at 565 nm). The practical use of an ‘ensemble’ was demonstrated by its application to the detection of iodide in the living cells.
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危险品化学品经营许可证（带存储）