查找使用阿拉丁产品的科研文献

Anthrax as a Biological Weapon: From World War I to the Amerithrax Investigation

René Pita,Rohan Gunaratna

Sequential recognition of Hg2+ and I− based on a novel BODIPY-salen sensor

Kai Liu,Ye Zhang,Ti Zhou,Xiaohe Liu,Bo Chen,Xin Wang,Xiaojun Zhao,Jianzhong Huo,Yajing Wang,Bolin Zhu

A novel multifunctional sensor based on the ‘privileged ligand’ salen scaffold conjugated with BODIPY was designed and synthesized. This conjugate displays a sequential recognition of Hg 2+ and I − with high selectivity and sensitivity via formation of three-component ensembles.These new approaches have been successfully applied for the detection of their corresponding targets in Haihe River with acceptable recovery.

A highly luminescent copper (I) halide complex scintillator for X-ray imaging application

Xun-Yang Chen,Jun-Hua Wei,Zi-Lin He,Jing-Hua Chen,Xuejing Wang,Liang Li,Dai-Bin Kuang

Copper (I) halide complexes have garnered much attention due to their straightforward synthesis, high luminescence efficiency, and structural diversity. In this work, a new class of Cu 2X2L2 complexes (X = Cl, Br, and I; L = 2-(dicyclohexyl)-2-(N, N-dimethylamino) biphenyl) were synthesized. Among them, the Cu 2I2L2 complex exhibits a broad emission band and a high photoluminescence quantum yield of nearly 100%. Time-dependent density functional theory calculations reveal that the emission of Cu 2I2L2 stems primarily from the mixed metal/halogen-to-ligand charge transfer state. Notably, the Cu 2I2L2 complex exhibits a light yield of 38297 photons MeV -1 and an ultra-low detection limit of 49.4 nGy s -1 . The scintillation film fabricated from Cu2I2L2 enables high-resolution X-ray imaging with a spatial resolution of 10 lp mm -1 . These results underscore the promising potential of Cu 2I2L2 as an excellent scintillator for advanced X-ray imaging applications.

Optimization of Chitosan/Modified Chitosan–Silver(I) Composite Film and Application to Strawberries

Jinhong Huang,Yiping Wang,Mengyuan Pang,Chengpeng Li,Pengzhi Hong,Zhang Hu

Fresh fruits are prone to spoilage due to microbial contamination and moisture loss, highlighting the need for effective packaging materials with strong barrier and antimicrobial functions. In this work, a bilayer composite film with antimicrobial and preservative properties was fabricated using the solution casting method, consisting of an inner chitosan (CS) layer and an outer complex layer of chitosan-2-pyridinecarboxaldehyde-Ag(I) (CS-PCA-Ag(I)). Preparation conditions were optimized via single-factor experiments combined with response surface methodology. The resulting composite film showed significantly enhanced mechanical properties, with tensile strength of 38.52 ± 2.07 MPa and elongation at break of 67.32 ± 1.47%, as well as a low water vapor permeability of 2.81 × 10−7g·m−1·h−1·Pa−1. It also exhibited strong antibacterial activity againstStaphylococcus aureusandEscherichia coli, with inhibition zone diameters of 19.8 ± 0.3 mm and 15.6 ± 0.2 mm, respectively. Strawberry preservation tests demonstrated that the CS/CS-PCA-Ag(I) film effectively suppressed microbial growth and reduced fruit weight loss (8.27 ± 0.42% after 5 days), thereby extending the shelf life of strawberries. Cytotoxicity evaluation and silver ion migration analysis further confirmed the film’s good biocompatibility and safety. Collectively, the CS/CS-PCA-Ag(I) composite film holds considerable promise for fresh food preservation applications.

Maternal PFBS exposure disrupts oocyte meiotic prophase I and reduces primordial follicle pool

Xueke Zhang,Tian Ma,Weijia Hu,Yao Yao,Guanjian Li,Peipei Guo,Cong Ma,Huifen Xiang,Pei Zhou

The establishment of the primordial follicle pool during embryogenesis determines the reproductive lifespan of female mammals, and disturbances during meiotic prophase I (MPI) can permanently compromise ovarian reserve. Perfluorobutanesulfonic acid (PFBS), a short-chain replacement for legacy perfluoroalkyl substances (PFAS), is increasingly used in industrial and consumer products, yet its effects on fetal oocyte development remain insufficiently understood. In this study, pregnant ICR mice were orally exposed to PFBS (20 or 200 mg/kg) from 13.5 to 17.5 days post coitum, corresponding to the critical period of meiotic initiation. Maternal PFBS exposure delayed meiotic progression, reflected by the accumulation of pachytene-stage oocytes, without apparent disruption of synapsis. However, PFBS-exposed oocytes showed increased γ-H2A.X, DMC1, and RAD51 foci, indicating impaired DNA double-strand break repair and homologous recombination. Consequently, the number of diplotene-stage oocytes and postnatal primordial follicles was significantly reduced. These findings suggest that prenatal PFBS exposure interferes with homologous recombination–mediated DNA repair during fetal oocyte meiosis, resulting in a diminished ovarian reserve. This work provides mechanistic evidence supporting the potential reproductive toxicity of emerging short-chain PFAS.

A label-free fluorescent aptamer sensor for testosterone based on SYBR Green I

Yucui Hou,Xiaoling Lu,Jie Yang,Chunhua Tang,Hanbing Jiang,Tongji Cai,Meilun Chen,Zheng Wei,Peng Yu

Testosterone is a steroid hormone that plays an indispensable role in the normal metabolism of organisms. However, exogenous testosterone, even as low as nmol L−1, will harm the human body due to accumulation. In this study, we developed an unlabeled fluorescent sensor for testosterone based on SYBR Green I. SYBR Green I is a fluorescent dye that can be embedded into the G-quadruplex of the testosterone aptamer T5. The fluorescence quenching effect is utilized to achieve quantitative detection, which occurs by the competition between testosterone and SYBR Green I for the T5 aptamer binding sites. In this work, we optimized the detection conditions to make the fluorescent sensor more sensitive and verify the specificity, linear range, and detection ability in the buffer and real water samples. The sensor's LOD and LOQ values were 0.27 nmol L−1 and 0.91 nmol L−1, respectively, while the detection range was linear from 0.91 nmol L−1 to 2000 nmol L−1. According to the results, the sensor shows high specificity and good performance even in real sample detection such as tap water and river water, providing an alternative method for the quantitative detection of testosterone in the environment, which is more convenient and efficient.

TPGS-Modified Long-Circulating Liposomes Loading Ziyuglycoside I for Enhanced Therapy of Myelosuppression

Tingting Song,Hong Wang,Yue Liu,Rongshan Cai,Dezhi Yang,Yongai Xiong

Background Ziyuglycoside I (ZgI), an active ingredient isolated from traditional Chinese medicine Sanguisorba officinalis L, has been demonstrated to increase the leucocytes and protect hematopoietic stem cells. However, the poor solubility and a short half-life of ZgI limit its bioavailability and efficacy. The D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) has been widely used to increase the solubility, improve the encapsulation rate, and extend the half-life of drugs.Methods Here, we formulated the TPGS-modified long-circulating liposomes loading ZgI with a sustained drug release and enhanced therapy for myelosuppression. ZgI-TPGS-liposomes were manufactured using a thin-film hydration technique, followed by characterizations of physicochemical properties, including the particle size, zeta potential, TEM, SEM, FTIR, XRD, stability, drug loading (DL), encapsulation efficiency (EE). The in vitro and in vivo delivery efficiency were further evaluated by cellular uptake, in vitro drug release and in vivo pharmacokinetics. Finally, therapeutic effect on myelosuppression was investigated.Results The ZgI-TPGS-liposomes had an particle size of 97.89 ± 1.42 nm and ZP of −28.65 ± 0.16 mV. It exhibited DL of 9.06 ± 0.76% and EE of 92.34 ± 3.83%, along with excellent storage stability, cellular uptake and sustained drug release to free ZgI and liposomes without TPGS. Additionally, the TPGS modified liposomes significantly enhanced the therapeutic effect of ZgI on CTX induced myelosuppression, which can be confirmed in the apoptosis inhibition and cell viability promotion of CTX injured HSPC-1 cells. Also, the mice in vivo pharmacodynamics demonstrated that TPGS liposomes promoted ZgI increasing the numbers of leucocytes and neutrophils in myelosuppression mice induced by CTX.Conclusion Our research suggest that TPGS-modified long-circulating liposomes loading ziyuglycoside I has potential application in myelosuppression therapy.

Reconfigurable Peptide Analogs of Apolipoprotein A-I Reveal Tunable Features of Nanodisc Assembly

Daiyun Xu,Xu Chen,Yongxiao Li,Zhidong Chen,Wanting Xu,Xinpei Wang,Yonghui Lv,Zhe Wang,Meiying Wu,Gang Liu,Junqing Wang

Nanodisc (ND)-forming membrane scaffold proteins or peptides developed from apolipoprotein A-I (apoA-I) have led to considerable promise in structural biology and therapeutic applications. However, the rationale and regularity characteristics in peptide sequence design remain inconclusive. Here, we proposed a consensus-based normalization approach through the reversed engineering of apoA-IΔ1–45 to design reconfigurable apoA-I peptide analogs (APAs) for tunable ND assembly. We present extensive morphological validations and computational simulation analyses on divergent APA-NDs that are generated by our method. Fifteen divergent APAs were generated accordingly to study the assembly machinery of NDs. We show that APA designs exhibit multifactorial influence in terms of varying APA tandem repeats, sequence composition, and lipid-to-APA ratio to form tunable diameters of NDs. There is a strong positive correlation between DMPC-to-APA ratios and ND diameters. Longer APA with more tandem repeats tends to yield higher particle size homogeneity. Our results also suggest proline is a dispensable residue for the APA-ND formation. Interestingly, proline-rich substitution not only provides an inward-bending effect in forming smaller NDs but also induces the cumulative chain flexibility that enables larger ND formation at higher lipid ratios. Additionally, proline-tryptophan residues in APAs play a dominant role in forming larger NDs. Molecular simulation shows that enriched basic and acidic residues in APAs evoke abundant hydrogen-bond and salt bridge networks to reinforce the structural stability of APA-NDs. Together, our findings provide a rational basis for understanding APA design. The proposed model could be extended to other apolipoproteins for desired ND engineering.

Growth of SnX2 (X = Br, I) Single Crystals with Self-Trapped Exciton Emission

Dayu Huang,Qiuyun Ouyang,Jinjiang Wu,Youchao Kong,Bo Wang,Hongzhou Lian,Jun Lin

Broadband emission with a large Stokes shift is important to obtain an excellent color rendering index of the solid-state lighting device. Among low-dimensional material and perovskite-like phosphors with broadband self-trapped emission, Sn-based phosphors have attracted much attention due to their high photoluminescence quantum yield (PLQY). However, the disadvantage is that the synthesis of Sn-based phosphors needs to be performed in a glovebox. Upon photoexcitation, the broadband emission of self-trapped excitons results from exciton–phonon coupling induced by the transient distortion of the lattice. Low-dimensional material structures often promote self-trapped emission because of more vibrational degrees of freedom and easier polarization under photoexcitation. Here, zero-dimensional (0D) SnX2 (X = Br, I) single crystals are synthesized by the solvent evaporation method in the air. SnX2 emits blue light, broadband yellow light, and deep red light, among which SnBr2 has the best luminescence performance. The photoluminescence quantum yield (PLQY) of SnBr2 reaches 85% and the Stokes shift reaches 265 nm. The PL intensity of SnX2 is linearly related to excitation power, which preliminarily indicates that the origin of SnX2 luminescence is attributed to self-trapped emission (STE). The white light-emitting diodes (WLEDs) were fabricated using yellow-emitting SnBr2 and blue-emitting BaMgAl10O17:Eu2+, which has a low correlated color temperature (3160 K) and a relatively common color rendering index (79).

Metabolic pathway assembly using docking domains from type I cis-AT polyketide synthases

Sun Xixi,Yuan Yujie,Chen Qitong,Nie Shiqi,Guo Jiaxuan,Ou Zutian,Huang Min,Deng Zixin,Liu Tiangang,Ma Tian

Engineered metabolic pathways in microbial cell factories often have no natural organization and have challenging flux imbalances, leading to low biocatalytic efficiency. Modular polyketide synthases (PKSs) are multienzyme complexes that synthesize polyketide products via an assembly line thiotemplate mechanism. Here, we develop a strategy named mimic PKS enzyme assembly line (mPKSeal) that assembles key cascade enzymes to enhance biocatalytic efficiency and increase target production by recruiting cascade enzymes tagged with docking domains from type I cis -AT PKS. We apply this strategy to the astaxanthin biosynthetic pathway in engineered Escherichia coli for multienzyme assembly to increase astaxanthin production by 2.4-fold. The docking pairs, from the same PKSs or those from different cis -AT PKSs evidently belonging to distinct classes, are effective enzyme assembly tools for increasing astaxanthin production. This study addresses the challenge of cascade catalytic efficiency and highlights the potential for engineering enzyme assembly.

Label-free detection of cardiac troponin-I with packaged thin-walled microbubble resonators

Zhihe Guo,Xuyang Zhao,Yi Zhou,Yuxiang Li,Zhiran Liu,Man Luo,Xiang Wu,You Wang,Meng Zhang,Xi Yang

The measurement of cardiac troponin-I (cTnI) is widely used for diagnosing acute myocardial infarction (AMI) diseases because of its myocardial specificity. Packaged microbubble resonators with thin wall are utilized for label-free and specific detection of cTnI based on whispering gallery mode (WGM). This packaged structure can provide a good protection for the biosensor, improve the anti-interference ability of the sensor and reduce the system noise. The theoretical detection limit of the biosensors for cTnI in phosphate buffer saline (PBS) is 0.4 ag mL −1 (0.02 a M ). Furthermore, we demonstrated that the biosensors can be used to detect cTnI molecules in simulated serum and the theoretical detection limit is also 0.4 ag mL −1 (0.02 a M ). These results are much far below the clinical cut-off value and show a huge application potential for the detection of cardiac biomarkers of AMI.

An interesting 3D energetic metal - framework based Ag(I) ions and 3,4-diaminofurazan

Xiaopeng Zhang,Yige Wu,Pengcheng Wang,Qiuhan Lin,Shusen Chen,Shaohua Jin,Yuangang Xu,Ming Lu

As a new structural motif, an interesting 3D energetic metal – framework of silver ions and 3,4-diaminofurazan, [Ag+(daf)NO3−]n, is successfully synthesized and the structure is determined by single-crystal X-ray diffraction. The effects of hydrogen bond, coordination bond, adsorption and filling interactions make this compound have a high density (2.694 g·cm−3 at 293 K). The calculated detonation velocity and the sensitivities are all comparable with trinitrotoluene (TNT). The onset decomposition temperature is equal to hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), state-of-the-art explosive. This framework provides a potential superiority of E-MOFs for energetic materials.