MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Beili Wang,Wei Gao,Liang Yang
An Exo-III-triggered target-reconstructed DNAzyme walker enables ultrasensitive multiplex miRNA detection. • Cascade amplification integrates Exo-III recycling, polymerase/endonuclease cycling, and DNAzyme walking. • Orthogonal Pt, Ir, Rh tags allow simultaneous ICP-MS quantification of three miRNAs without spectral cross-talk. • Detection limits reach 0.15 fM (miR-21), 0.32 fM (miR-1246), and 0.26 fM (miR-20a) with excellent linearity. • The method discriminates single-base mismatches and performs well in diluted human serum (92–108% recovery). Cervical cancer remains a leading cause of cancer-related mortality among women, and the prognosis of patients after radiotherapy varies considerably due to differences in tumor radiosensitivity and treatment response. Reliable biomarkers for post-radiotherapy prognosis are therefore critically needed. MicroRNAs (miRNAs) have emerged as promising non-invasive indicators, yet accurate detection is hindered by their low abundance and the lack of multiplexing capability in conventional methods. Herein, we report a highly sensitive and specific platform for simultaneous detection of three cervical cancer radiotherapy-associated miRNAs (miR-21, miR-1246, and miR-20a) by integrating an Exo-III-triggered target-reconstructed DNAzyme walker with inductively coupled plasma mass spectrometry (ICP-MS). The strategy employs a structurally locked DNA walker that is sequentially activated through Exo-III-mediated target recycling and DNA polymerase/endonuclease-assisted cyclic amplification, ultimately unleashing a DNAzyme walker to cleave element-labeled substrates. Using orthogonal platinum group element tags (Pt, Ir, Rh), the released tags are quantified by ICP-MS without spectral cross-talk. The method achieves detection limits as low as 0.15 fM, 0.32 fM, and 0.26 fM for miR-21, miR-1246, and miR-20a, respectively, with excellent single-nucleotide discrimination and negligible cross-reactivity. Successful spike-recovery experiments in diluted human serum validate its practical feasibility. This cascade amplification strategy offers a robust, multiplex, and ultrasensitive tool for non-invasive liquid biopsy-based prognostic assessment of post-radiotherapy cervical cancer, and can be readily adapted for other disease-related miRNA panels. Download: Download high-res image (121KB) Download: Download full-size image
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DOI
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2022
·Vol. 48
·期 6
·2557-2573
·
IF 3.134
Liu Yueyu,Yin Zhili,Wang Ziqing,Mou Ronglin,Wei Zhong
High surface area ZrO 2 –KOH sample was prepared and used the catalyst for the synthesis of glycerol carbonate (GC) from dimethyl carbonate (DMC) and glycerol. The structure properties of ZrO 2 –KOH were characterized by XRD, BET, CO 2 -TPD, XPS, and ICP-OES. It was found that the strong basicity of ZrO 2 –KOH might be attributed to the oxygen vacancies as well as the big surface area. Experiments were developed to evaluate the effects of catalysis loading, proportion of reactants, temperature and reaction time on the conversion of glycerol to GC. The consequences showed that ZrO 2 –KOH was a highly efficient basic catalyst for synthesis of GC from glycerol. The catalytic performance of ZrO 2 –KOH is much better than that of ZrO 2 –KOH–CP, ZrO 2 –NH 4 OH, and some reported heterogeneous catalysts. And the higher performance of ZrO 2 –KOH was ascribed to the strong basicity. 99.43% conversion was obtained in a particular situation of catalyst/glycerol weight ratio of 3 wt%, DMC/glycerol molar ratio of 3:1, reaction temperature of 80 °C, and reaction time of 2 h. The plausible reaction mechanism for the transesterification on the strong basic active sites was discussed.
研究领域:
催化
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化学工程
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材料科学
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高影响因子
Toxics
·
2024
·Vol. 12
·期 5
·325
·
IF 3.9
Ge Song,Honglian Tan,Chuhan Cheng,Peng Li,Xinyang Sun,Yuling Zhou,Yong Fang
Hexavalent chromium (Cr(VI)) is known as the most hazardous species of chromium. Speciation analysis of Cr in foods is of a great significance for assessing its influences on human health. In this study, a fast HPLC-ICP-MS method for the determination of Cr(VI) was developed for determining the content of Cr(VI) and also investigating its transformation in foods. The developed method employs an alkali extraction and weak anion-exchange column separation for distinguishing the Cr species, facilitating accurate Cr(VI) quantification within 1.5 min. This technique was applied to determine the Cr(VI) levels in a range of food products, including yoghurt, milk powder, rice flour, orange juice, green tea, white vinegar, and whole wheat bread. The results showed that no Cr(VI) was detected in these food products. Spiking experiments revealed that the recovery rate of Cr(VI) decreased with the increase in its contact time with food products. A further exploration of Cr(VI) in various food components such as vitamin C, tea polyphenols, whey proteins, gelatin, fructose, and cellulose indicated the conversion of Cr(VI) to organic Cr(III) over a period from 20 min to 60 h. It was found that high temperatures and acidic conditions accelerated the rate of Cr(VI) conversion to organic Cr(III) in the six food components mentioned above. This evidence suggests that natural reducing substances in foods probably prevent the occurrence of Cr(VI).
研究领域:
分析化学
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食品科学
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环境健康
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DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2024
·
IF 5.6
Yuke Hou,Yangjian Hu,Min Li,Jiahui Nong,Fengyuan Xie,Yuhan Fan,Jianhao Zhang,Xianming Zeng,Minyi Han,Xinglian Xu,Xia Wang
The cold plasma (CP) technique was applied to alleviate the contamination of polycyclic aromatic hydrocarbon (PAH) in this investigation. Two different CP treatments methods were implemented in the production of beef patties, to investigate their inhibition and degradation capacity on PAHs. With 5 different cooking oils and fats addition, the inhibition mechanism of in-package cold plasma (ICP) pretreatment was explored from the aspect of raw patties fatty acids composition variation. The results of principal component analysis showed that the first two principal components accounted for more than 80 % of the total variation in the original data, indicating that the content of saturated fatty acids was significantly positively correlated with the formation of PAHs. ICP pretreatment inhibited the formation of PAHs by changing the composition of fatty acids, which showed that the total amount of polyunsaturated fatty acids decreased and the total amount of monounsaturated fatty acids increased. Sensory discrimination tests demonstrated there were discernable differences between 2 CP treated samples and the controls, utilization of the ICP pretreatment in meat products processing was expected to achieve satisfying eating quality. In conclusion, CP treatment degraded PAHs through stepwise ring-opening oxidation in 2 reported pathways, the toxicity of PAHs contaminated products was alleviated after CP treatment.
研究领域:
食品科学
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环境化学
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等离子技术
·
肉类加工
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产品关联
DOI
高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2020
·
IF 1.701
Long Wang,Zhiming Wang,Cuo Zhou,Weijun Song,Chunyan Sun
A cesium potentiometric microsensor based on magnetic ion-imprinted polymer (MIIP) has been developed for Cs(I) sensing. The core-shell structure Cs(I)-MIIP was synthesized by surface-imprinting technique, using functionalized magnetic Fe3O4@SiO2 microspheres, Cs(I) ion and carboxymethyl chitosan as supporter, template and functional monomers respectively. The resultant Cs(I)-MIIP was used as ionophore in the construction of the PVC membrane electrode to obtain a selective potentiometric microsensor toward Cs(I) ions. The fabricated sensor exhibited a highly sensitive Nernstian response (60.2 mV/decade) over the concentration range of 1 × 10−6 to 1 × 10−1 M of Cs(I). The response time of the microsensor was considerably short (less than 3 s) and its detection limit was determined as 3.0 × 10−7 M. The microsensor was effectively performed in the pH range of 4–6, and revealed desirable selectivity over a wide variety of other cations including alkali, alkaline earth and heavy metals. The proposed microsensor was also used for the determination of Cs(I) contents in the environmental water samples. The potentiometric results were in good harmony with the results obtained by the ICP-MS method.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
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2018
·
IF 3.956
Qilei Yang,Ang Cao,Na Kang,Kang An,Zhao-Tie Liu,Yuan Liu
The catalyst for direct ethanol synthesis (DES) from syngas was investigated in this work, which is Co/La 2 O 3 -doped La 4 Ga 2 O 9 . The catalyst was prepared by using LaCo 1−x Ga x O 3 with perovskite phase as the precursor to intensify the interaction in the elements of Co, La and Ga. The catalysts were characterized by using XRD , H 2 -TPR, TEM , EDS, XPS , BET, ICP and TG techniques. This catalyst showed high selectivity to ethanol and excellent stability including excellent resistance to sintering and carbon deposition . It seems that the ethanol is generated in the interface between metallic Co and La 4 Ga 2 O 9 , and in the interface cobalt would be positively charged due to the interaction between Co nanoparticles and La 4 Ga 2 O 9 . The results and the related discussions indicate that Co/La 2 O 3 -doped La 4 Ga 2 O 9 catalyst is a promising catalyst for DES from syngas. Graphical abstracts.
研究领域:
催化
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化学工程
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材料科学
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DOI
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
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2011
·Vol. 26
·期 6
·1191-1197
·
IF 4.372
Jing-Min Liu,Xiu-Ping Yan
An ultrasensitive method for simultaneous determination of cytochrome c (cyt-c) and insulin was developed by combining aptamer-based bioassay and immunoassay, multielement-tagging and inductively coupled plasma mass spectrometry (ICP-MS). Aptamer-modified gold nanoparticles (apt-AuNPs) and antibody-modified silver nanoparticles (ab-AgNPs) were employed as specific element tags for cyt-c and insulin, respectively. The prepared surface-functionalized magnetic microparticles (MMPs) were used for efficient and fast magnetic separation. The bioassay conditions were carefully optimized, including the amount of MMPs, the concentration of AuNPs and AgNPs, and the reaction time. Under optimal conditions, the developed method gave a linear range of 0.1–20 nM for cyt-c and 0.2–40 nM for insulin, a detection limit (3s) of 1.5 fmol (30 pM in 50 μL) for cyt-c and 5.5 fmol (110 pM in 50 μL) for insulin. The precision (relative standard deviation) for six replicate determinations of cyt-c (0.6 nM) and insulin (2.0 nM) was 6.6%, and 6.0%, respectively. The present method exhibits good specificity with recoveries from 87% to 98% for spiked cyt-c and insulin in human serum samples. The methodology demonstrated here provides a new possibility for bioassays and clinical diagnoses, which has potential for simultaneous determination of two or more low-abundance biomarkers of interest via multi-element tags.
研究领域:
生物分析化学
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分子诊断
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纳米生物技术
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临床检验医学
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蛋白质组学
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DOI
高影响因子
ANALYTICAL LETTERS
·
2021
·
IF 2.329
Zhaoxue Zhang,Yi Lu,Haipu Li,Nan Zhang,Junfei Cao,Bo Qiu,Zhaoguang Yang
The simultaneous separation and determination of inorganic trivalent antimony Sb(III) and pentavalent antimony Sb(V) in plant, soil, and sediment samples was carried out by high performance liquid chromatography – inductively coupled plasma – mass spectrometry (HPLC-ICP-MS). The chromatographic conditions (composition and concentration of competing ion, pH value and flow rate of mobile phase) and the operating parameters for inductively coupled plasma – mass spectrometry were optimized by single factor experiments. The separation of the Sb species was achieved within 6 min using the optimized chromatographic conditions. The detection limits of the method were 0.016 μg/L and 0.021 μg/L for Sb(III) and Sb(V), respectively. The retention times of Sb(V) and Sb(III) were 2.7 min and 4.5 min, respectively. The relative standard deviations for replicate measurements (n = 6) of low (5 μg/L), medium (20 μg/L), and high (40 μg/L) concentrations of Sb(III) and Sb(V) were less than 4%. The results of spiking and recovery experiments for the extracting solutions of soil, sediments, and plants showed that the spiked recoveries of the two species ranged from 92% to 103%. This method has been used to determine the antimony species in extracts of environmental samples.
研究领域:
环境科学
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分析化学
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高影响因子
ASIAN JOURNAL OF ANDROLOGY
·
2019
·
IF 2.862
Tao Li,Fu-Dong Fu,Chang-Jing Wu,Feng Qin,Run Wang,Jiu-Hong Yuan
This study aimed to explore whether and how anti-lysyl oxidase (anti-LOX) combined with a vacuum device (VD) could promote penile lengthening and to evaluate the effect on erectile function. This study was performed on four groups of adult rats: control, anti-LOX, VD (negative pressure value of −300 mmHg), and anti-LOX + VD. Penile length was measured by a modified VD method and verified on exposed length data. Intracavernous pressure (ICP) and maximum ICP/mean arterial pressure (MAP) ratio were recorded to assess erectile function. For corpus cavernosum, LOX activity and concentrations of pyridinoline, desmosine, hydroxyproline, and elastin were analyzed; transmission electron microscope and Hart's elastin staining were performed to monitor microstructural changes. Anti-LOX and VD significantly lengthened the penis by 10.8% (3.75 mm) and 8.2% (2.48 mm) compared with the control group, respectively, while anti-LOX + VD achieved the longest penile size (40.58 ± 0.40 mm) which was 17.4% longer than the control group (34.58 ± 0.54 mm). After 1-week washout, no penile retraction was observed. Meanwhile, exposed penile length data confirmed that the penis in the anti-LOX + VD group was also significantly longer. Anti-LOX inhibited LOX activity to reduce pyridinoline level, which led the penile tunica albuginea remodeling. However, it had no effect on hydroxyproline, desmosine, and elastin levels. Moreover, anti-LOX had no impact on erectile function, which was determined by ICP and ICP/MAP ratio. These results suggest that anti-LOX elongates the penis by reducing pyridinoline, which induces tunica albuginea remodeling. This lengthening effect was more obvious when combined with a VD. All procedures had no impact on erectile function.
研究领域:
泌尿学
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药理学
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生物医学工程
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Guangyang Xiao,Beibei Chen,Man He,Xiaoting Li,Bin Hu
In this work, a simple, sensitive and specific assay for DNA was proposed by combining inductively coupled plasma mass spectrometry (ICP-MS) detection with gold nanoparticle (AuNPs) amplification and isothermal circular strand-displacement polymerization reaction (ICSDPR). First, AuNPs were decorated with hairpin-structured DNA (HP-DNA) through Au–S bond to form the AuNPs probe. The ICSDPR was conducted on AuNPs probe in a homogeneous phase to realize the dual amplification and simplify the analytical process at the same time. By using a biotin modified primer, AuNPs were connected with biotins after the ICSDPR, then captured by the streptavidin modified magnetic beads (SA-MBs), and finally detected by ICP-MS. Many key factors including probe volume, hybridization time, polymerase amount, primer concentration, enzyme reaction time, SA-MBs capture time and desorption time were optimized. Under the optimized condition, the proposed method could detect target DNA as low as 45 zmol (8.9 fM in 5 μL) in a relative short time (about 4.5 h) with good specificity, and the linear range of this method is 0.1–10000 pM, the relative standard deviations are in the range of 3.6–6.4%. The proposed method was applied for determination of target DNA in human serum samples, the recovery for the spiked human serum samples is in the range of 84–120%. It demonstrates a good application potential of the developed method for biological studies and clinical diagnosis of human pathogenic diseases.
研究领域:
分析化学
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纳米技术
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生物医学工程
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分子生物学
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临床诊断
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yamin Xiong,Lihong Zhou,Hui Wang,Huiling Li,Yue Hu,Peijun Meng,Xueying Zhang,Peili Huang
Monitoring the level of exchangeable copper (CuEXC) and ceruloplasmin (Cp) in serum is of great significance for Wilson’s disease (WD). In this work, a facile one-step competitive immunoassay for rapid and simultaneous detection of CuEXC and Cp has been developed based on antigens coated magnetic nanoparticles (immuno-MNPs) and antibodies functionalized quantum dots (QDs-Abs). Just by mixing immuno-MNPs, with diluted serum and QDs-Abs together, the antigens on immuno-MNPs compete with the target in serum and bind to QDs-Abs, thereby causing the changes in fluorescence intensity of QDs. Under selected conditions, this one-step method exhibited excellent precision and selectivity with the measured limit of detection of 0.2 nM and 7.0 μg/L for CuEXC and Cp, respectively. Most importantly, this method was successfully applied to detect CuEXC and Cp without the usual time-consuming pretreatment. The results showed no significant difference ( P >0.01) from those of ICP-AES and ELISA; moreover, CuEXC significantly decreased to a normal level after the treatment of preliminarily diagnosed WD patients, while the changes of Cp level is not significant ( P > 0.01). Therefore, the proposed one-step competitive immunoassay has great potential applications in the rapid laboratory diagnosis of WD with distinct advantages over the existing methods in terms of multi-target, accuracy, facile, fast and cost-effectiveness.
研究领域:
医学诊断
·
纳米技术
·
临床化学
·
免疫学
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产品关联
DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 5
·638-644
·
IF 0
Jiang Pengfei,Haji Chomba,Ye Xiufang,Chang Menglei,Li Wenjun,He Xuliang,Chen Dongchu,Nie Libo
An inductive coupled plasma mass spectrometry (ICP-MS) method based on gold nanoparticles (AuNPs) and bio-barcode signal amplification was presented for the sensitive detection of gastric cancer related gene. The target DNA, magnetic nanoprobes and SiO2/AuNPs barcode probes
were hybridized to form a "sandwich" structure, then a large amount of AuNPs which were amplified by bio-barcode technology were detected by ICP-MS. This method presented a limit of detection as low as 1 fM. The ratio of the background-subtracted 197 Au signals for totally complementary
DNA, single-base mismatched DNA, double-base mismatched DNA and totally mismatched DNA was 115:30:16:1, respectively. It's suggested that the complementary and mismatched DNA can be distinguished clearly. This novel ICP-MS biosensor is prospective in DNA detection with high sensitivity and
specificity.
研究领域:
分析化学
·
分子生物学
·
癌症研究
·
纳米技术
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DOI
危险品化学品经营许可证（带存储）