Scientific Reports
·
2016
·Vol. 6
·期 1
·1-9
·
IF 5.228
Wang Jun,Zhu Xuexia,Huang Xin,Gu Lei,Chen Yafen,Yang Zhou
Obscure puffer Takifugu obscurus , a species of anadromous fish, experiences several salinity changes in its lifetime. Cadmium (Cd) is a toxic heavy metal that can potentially induce oxidative stress in fish. The present study aimed to detect the combined effects of Cd (0, 5, 10, 20 and 50 mg L −1 ) and salinity (0, 15 and 30 ppt) on juvenile T. obscurus . Results showed the juveniles could survive well under different salinities; however, with Cd exposure, the survival rates significantly decreased at 0 and 30 ppt. At 15 ppt, tolerance to Cd increased. Cd exposure clearly induced oxidative stress and the responses among different tissues were qualitatively similar. Salinity acted as a protective factor which could reduce the reactive oxygen species and malondialdehyde levels. In addition, salinity could enhance the antioxidant defense system, including superoxide dismutase, catalase and glutathione. Na + /K + –ATPase activity significantly decreased under Cd exposure in gill, kidney and intestine. These findings indicated that Cd could moderate the adaptability of juvenile T. obscurus to high salinity and low salinity played a protective role upon Cd exposure. Thus, the role of salinity should be considered when evaluating the effect of heavy metals on anadromous and estuarine fishes.
研究领域:
环境毒理学
·
鱼类生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
·
IF 2.694
Kangling Yin,Li Chen,Feng Liu,Taotao Fan,Zongcheng Yan
Aiming at developing more biocompatible and economic separation platforms, aqueous biphasic systems (ABS) composed of deep eutectic solvents (DES) and tert-butanol were proposed for the first time. The effects of key factors such as the hydrogen-bond donor (HBD) properties and temperature on the phase-forming ability of DES/tert-butanol ABS were investigated. Meanwhile, syringic acid and eugenol mixtures were selected as model biomolecules to evaluate the efficiency of DES/tert-butanol ABS to use as liquid–liquid extraction systems. The results indicated that DES components migrated preferentially to the bottom phase while tert-butanol was concentrated in the top phase. The more hydrophilic HBD or lower temperature was responsible for greater efficiency to induce the formation of DES/tert-butanol ABS. The reason for phase separation was the incompatibility of the hydrogen-bond acceptor (HBA) and HBD with tert-butanol in aqueous solution, where the higher hydrophilic HBD enriched favorably in the lower phase, allowing for the formation of the DES/tert-butanol ABS. The selective separation of syringic acid and eugenol was a consequence of the HBD characters, temperature, and tie-lines length of the ABS, and they could be greatly separated by using the ABS composed of betaine-xylitol (DES) and tert-butanol at 298.15 K. The highest extraction efficiency of syringic acid and eugenol was 91.64 and 97.62%, respectively. Further, syringic acid and eugenol as well as DES can be recovered and reused by using water as the antisolvent and the vacuum distillation method. This study may be expected to lay a solid foundation for the expansion of DES-based ABS and selective separation of similar biomolecules.
研究领域:
化学工程
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2014
·
IF 2.045
Xia Gui,Wei Wang,ChenWei Wang,Ling Zhang,Zhi Yun,Zhg Tang
The solubility of CO2 in polyethylene glycol dimethyl ether (NHD), octamethyl cyclotetrasiloxane (D4), and diethyl succinate (DS) was determined at the temperature variations from 285.19 K to 313.26 K up to 6 MPa by the constant-volume method. Then, Henry’s law constant (H) based on mole fraction was obtained by linear fitting of the vapor–liquid phase equilibrium data, and some thermodynamic properties of solutions were also calculated from the correlation of H. The values of H in NHD, D4, and DS vary from 2.5 MPa to 8 MPa and increase in the following order: DS < D4 < NHD, which is identical to the order of the solvents ability to dissolve CO2. Additionally, Henry’s law constant increases with temperature increase to confirm the solubility of CO2 decrease in the corresponding solvents for the pressure range studied.
研究领域:
化学工程
·
热力学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Li-Rong Guo,Guo-Liang Nie,Rui-Zhi Cui,Wu Li,Yong-Ming Zhang
In addition to the common sodium salt, potassium salt, magnesium salt, and sulfate, salt lake brine is also rich in trace components in varying degrees, such as lithium, bromine, rubidium, cesium, and so forth, which is of great industrial value. These trace components are further concentrated and enriched in old brine during the process of evaporation and concentration of brine resources. In view of the coexistence characteristics of lithium, potassium, bromine, and other ions in old halide, the phase equilibria of quinary system Li+, Na+, K+/Br–, and SO42––H2O were studied by the isothermal dissolution equilibrium method at 298.15 K. The solubility data of the equilibrium liquid phase were measured, and the compositions of the solid phase as well as the crystal forms of the equilibrium solid phase were identified when the equilibrium was achieved. The corresponding 3D and dry-base phase diagrams (Na2SO4·10H2O, K2SO4, LiBr·2H2O, Li2SO4·H2O, KBr, and NaBr·2H2O saturated) were drawn based on the phase equilibrium experimental data. The research results of phase equilibria and phase diagrams reveal that the phase relations and crystallization–dissolution law of each component in solution lay a certain foundation for further research on multi-temperature phase equilibrium and thermodynamic properties of the high-component brine system and to provide basic thermodynamic data for guiding the rational development and comprehensive utilization of brine resources.
研究领域:
化学
·
材料科学
·
资源与环境科学
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Qinghai Sun,Zhibo Zheng,Xiaolu Yang,Yanxia Zhao,Zhou Luo,Chao Wang,Yanhua Song,Haifeng Zou,Shihou Sheng
Herein, a variety of single-phase full-spectrum emission phosphors doped with Eu 2+ were synthesized. Under the 350 nm excitation, as K + and Y 3+ were introduced into Ca 10 Na(PO 4 ) 7 (CNP):1.5%Eu 2+ , respectively, the emission color of Ca 9.85 K y Na (1- y ) ( PO 4 ) 7 :1.5%Eu 2+ (0 ≤ y ≤ 1.0) was tuned from yellow to cyan, while that of Ca (9.85- z ) NaY 0.667 z (PO 4 ) 7 :1.5%Eu 2+ (0 ≤ z ≤ 1.0) was tuned from yellow to green. The internal quantum yield (QY) of Ca 9·85 K 0·4 Na 0·6 (PO 4 ) 7 (CKNP):1.5%Eu 2+ and Ca 9·45 NaY 0·267 (PO 4 ) 7 (CNYP):1.5%Eu 2+ reached 47.47 % and 27.11 %, respectively. The introduction of K + or Y 3+ made Eu 2+ selectively occupy the Ca sites and led to changes in the local environment of Eu 2+ , allowing for spectral tuning emission. In addition, the luminescence intensity of the samples retained 54.59 % and 31.34 % of their room temperature values. Eventually, two white light emitting diode (WLED) devices were fabricated, combining the synthesized white emitting phosphors with 365 nm ultraviolet (UV) chips. They exhibited high color rendering indices of 84.7 and 77.0, along with low correlated color temperatures of 5435 K and 5023 K. The Commission Internationale de L'Eclairage (CIE) color coordinates were (0.3349, 0.3975) and (0.3517, 0.4366), respectively. The results pointed to the phosphors' potential suitability for use in the WLED sector.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Jie Wang,Haibao Liu,Defang Ma,Yan Wang,Guangping Yao,Qinyan Yue,Baoyu Gao,Shue Wang,Xing Xu
In this work, the combined ultraviolet ozone process (UV/O 3 ) was applied for organic contaminant (Reactive Blue K-GL, RB) degradation in high salinity . The degradation rates of RB in both O 3 and UV/O 3 systems were enhanced by NaCl (the k increased from 0.080 to 0.116 to 0.132 and 0.267 min −1 respectively), while mineralization rate varied at different salt conditions. In addition, UV irradiation promoted the degradation efficiency of RB with the presence of salt. Singlet oxygen ( 1 O 2 ) was the primary active species in the UV/O 3 system. The quenching experiments and signal intensity of 1 O 2 corresponded well to the mineralization of RB. Under conditions of high salinity and high pH, O 3 has high mass transfer coefficient (k La , 3.303 min −1 ) and self-decomposition (k d , 0.600 min −1 ), which further promoted the formation of 1 O 2 for mineralization of RB. Furthermore, UV/O 3 system was efficient in real textile wastewater treatment (COD Cr removal rate 91.7% and decolorization rate 98.7%).
研究领域:
环境科学
·
水处理
·
污染物降解
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 26
·39027-39040
·
IF 5.19
Zhou Yiming,Elchalakani Mohamed,Liu Houfeng,Briseghella Bruno,Sun Chuanzhi
Since the advent of photocatalytic degradation technology, it has brought new vitality to the environmental governance and the response to the energy crisis. Photocatalysts harvest optical energy to drive chemical reactions, which means people can use solar energy to complete some resource-consuming activities by photocatalysts, such as environmental governance. In recent years, researchers have tried to combine photocatalyst TiO 2 with building materials to purify urban air and obtained good results. One of the important functions of photocatalysts is to degrade organic pollutants in water through light energy, but this technology has not been reported in the practical application areas. To extend this technology to practical application areas, photocatalytic concrete for degrading pollutants in waters was proposed and demonstrated for the first time in this paper. The photocatalytic concrete proposed based on the K-g-C 3 N 4 shows a strong ability to degrade the organic dyes. According to the experiment results, the angle of light source plays an important role in the process of photocatalytic degradation, while waters with pH value of 6.5–8.5 hardly influenced the degradation of organic dyes. When the angle of light source is advantageous for photocatalytic concrete to absorb more visible light, more organic dyes will be degraded by photocatalytic concrete. The degradation rate of methylene blue could reach about 80% in ½ hour under desirable conditions and is satisfied compared with that of reported works. This study implicates that photocatalytic concrete can effectively degrade organic dyes in water. The influences of changes in the water environment hardly affect the degradation of organic pollutants, which means photocatalytic concrete can be widely used in green infrastructures to achieve urban sewage treatment.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 11
·2420-2429
·
IF 3.366
Cao Jinping,Cheng Yuanzhi,Xu Bocheng,Wang Yizhen,Wang Fengqin
To ensure reasonable intake of selenium for humans and provide insights on the development and nutritional analysis of high-quality selenium-enriched food, we established a reliable and highly sensitive method for the determination of selenium species in Z0206 selenium-enriched polysaccharide (Se-EPS). By optimizing the pre-treatment conditions (different enzymes, enzyme combinations, enzymatic time, concentration of enzymes, and centrifugation time), we found that Se-EPS can be effectively hydrolyzed by proteinase K for 18 h, followed by HPLC-ICP-MS determination. The developed method presented high sensitivity with the limit of detection and quantitation in the range of 0.44–2.35 μg L −1 and 1.54–7.21 μg L −1 . The relative standard deviation (RSD) was all less than 10% and recovery was greater than 80% except for SeCys2, which further validated the repeatability and accuracy of the proposed method. Under the optimized conditions, we could determine 4 selenium species: elemental selenium (78.37%), seleno-methionine (14.27%), selenocystine (0.03%), and Se-methylselenocysteine (5.22%) in Se-EPS. This work provides new insights into the determination of different selenium species in selenium-enriched food.
研究领域:
分析化学
·
营养科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Liang Yan,Yongqi Ke,Yuhe Kan,Dao Lin,Jingkui Yang,Yujian He,Li Wu
The isomerization of l -amino acids in peptides and proteins into d -configuration under physiological conditions would affect the physiological dysfunction and caused protein conformational diseases. The presence of d -amino acids might change the higher-order structure of proteins and triggered abnormal aggregation. In order to better understand this phenomenon and promote degradation, we systematically studied the enzymatic hydrolysis of a series of peptides obtained by replacing l -amino acids in different positions of template peptide KYNETWRSED with d -amino acids under the action of Protease K. The results showed that, compared with normal peptide, isomerization of different amino acids had different effects on the anti-enzymatic hydrolysis of the peptides, especially d -tryptophan at position 6, which significantly inhibited enzymatic hydrolysis. The analysis of the peptide cleavage site revealed that the efficiency of enzymatic hydrolysis mainly depended on the isomerization of the amino acids at a specific site of the peptide cleavage. Further studies showed that the enzymatic hydrolysis of substrates could be facilitated by optimized reaction conditions such as temperature, pH, addition of metal ions, and change of buffer. In this way the accumulation of disease-associated d -amino acid containing polypeptides/proteins could be prevented.
研究领域:
生物化学
·
分子生物学
·
蛋白质化学
✓ 已验证
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Shuaishuai Cui,Cunxin Guo,Liang Yan,Yujian He,Li Wu
The incorporation of D-amino acids can influence the structure and enzymatic stability of proteins or peptides, especially when they are introduced at enzyme cleavage sites, significantly inhibiting the enzymatic hydrolysis of peptides. The abnormal accumulation of these peptides has been linked to age-related conditions, including cataracts and Alzheimer's disease. N-terminal acetylation, an essential post-translational modification, significantly enhances the physicochemical properties of peptides and plays an essential role in regulating their performance within biological systems. This research examined the impact of N-terminal acetylation on the enzymatic hydrolysis of peptides incorporating D-amino acids. Enzymatic activity assessments showed that N-terminal acetylation greatly promoted the enzymatic breakdown of these peptides by Proteinase K (PROK), with the substrate decay rate constant for the acetylated peptide Ac-6-w increasing by 17.5 times. This enhancement was specific to serine-type proteases, which exhibited a comparable cleavage pattern. Molecular docking further demonstrated that N-terminal acetylation improved interactions within the catalytic triad of serine proteases, leading to faster enzymatic degradation. The results provide novel insights into the impact of N-terminal acetylation on the enzymatic behavior of peptides incorporating D-amino acids, and they propose a potential approach for targeting these peptides to preserve normal physiological functions.
研究领域:
生物化学
·
医学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Cuiting Yang,Yingying Zhang,Mingyue Jia,Qingsong Qu,Zhixun Li,Qing Zhou,Minfang Feng,Yuting Tian,Weishuo Ren,Xinyuan Shi
Monascus fermented Ginseng exhibits superior lipid-regulation activity compared to the raw materials, which undoubtedly related to fermentation-induced changes. This study aims to elucidate the interactions between Monascus purpureus (M. purpureus) and ginseng during fermentation and identify an effective ginseng fraction optimal for co-fermentation. Initially, the effective fractions extracted from ginseng—total polysaccharides, amino acids, and ginsenosides, were separately added to the basic medium to assess their impact on M. purpureus growth and metabolism. Meanwhile, ginsenoside transformation during fermentation was also investigated. The fermentation product derived from the selected ginseng fraction and M. purpureus was assessed for its lipid-lowering efficacy. The results indicated that total amino acids and total ginsenosides, except for total polysaccharides, facilitated the growth of M. purpureus and enhanced Monacolin K production. The expression levels of genes associated with M. purpureus metabolism exhibited significant alterations. The changes in various ginsenoside monomers revealed potential ginsenoside transformation pathways during the fermentation process. Additionally, Monascus purpureus fermented Ginsenosides exhibited lipid-lowering efficacy comparable to Monascus fermented Ginseng, potentially regulating the lipid metabolism via the Peroxisome proliferator-activated receptor and Sterol regulatory element-binding protein (PPAR/SREBP) signaling pathway. These insights not only elucidate the interaction mechanisms between M. purpureus and ginseng fractions during fermentation but also demonstrate the lipid-lowering potential of Monascus purpureus fermented Ginsenosides at both cellular and molecular levels, highlighting certain significance for the rational utilization of ginseng resources, revealing the key role of microorganisms in the fermentation process.
研究领域:
微生物学
·
药理学
·
代谢生物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yupeng Cao,Xiaohan Zhou,Xiaoli Liu,Mei Yang,Wentao Liu,Dong Han
Fluorine-19 (19F) MRI (19F-MRI) is a promising method for quantifying biomedical research and clinical applications without background interference. Nevertheless, dependency on high-field MRI systems limits the applicability of 19F-MRI. Low-field MRI systems are more common than high-field MRI systems. Hence, developing 19F-MRI at low-field MRI devices can promote the 19F-MRI translation in medical diagnosis. The detection sensitivity of fluorine agents is critical in 19F-MRI. Reduction of the 19F spin-lattice relaxation time (T1) enables an improved detection sensitivity while requiring ultrashort echo time (UTE) imaging methods to reduce the negative spin–spin relaxation (T2) decay effect. However, conventional UTE sequences require hardware with high performance. Herein, we introduce the k-space scaling imaging (KSSI) MRI sequence that accomplishes sampling k-space with variable scales to implement hardware-friendly UTE 19F-MRI compatible with low-field MRI systems. We implemented experiments with swine bone, a perfluorooctyl bromide (PFOB) phantom, and one tumor-bearing mouse on two self-customized low-field MRI systems. The swine bone imaging validated the ultrashort TE of KSSI. Under high concentrations of manganese ferrite, a high signal-to-noise ratio was shown in the imaging of a fluorine atom concentration of 658 mM, which indicated high-sensitivity detection of KSSI. Moreover, the KSSI sequence exhibited a 7.1 times signal-to-noise ratio of spin echo sequence on the PFOB phantom imaging with a fluorine atom concentration of 3.29 M. Additionally, the various concentrations of the PFOB phantom imaging revealed quantifiable capacity. Finally, the 1H/19F imaging was implemented with KSSI on one tumor-bearing mouse. This method provides the potential for clinical translation of fluorine probes at low-field MRI systems.
研究领域:
医学影像
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）