Scientific Reports
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2017
·Vol. 7
·期 1
·1-11
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IF 4.259
Zhou Yang,Yuan Shaofei,Liu Qian,Yan Dandan,Wang Yun,Gao Li,Han Juan,Shi Haifeng
In this paper, an efficient and convenient Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles that applied to purify and immobilize his-tagged β-glucosidase was synthesized, in which, Fe 3 O 4 /PMG (poly ( N, N’- methylenebisacrylamide-co-glycidyl methacrylate) core/shell microspheres were synthesized firstly using distillation-precipitation polymerization, then iminodiacetic acid (IDA) was used to open epoxy rings on the shell of microspheres to the combination of Ni 2+ . The gene of β-glucosidase that was from Coptotermes formosanus Shiraki was amplified, cloned into the expression vector pET28a with an N-terminal His-tag, and expressed in E.coli BL21. The nanoparticles showed the same purification efficiency as commercial nickel column which was a frequently used method in the field of purifying his-tagged proteins from crude cell lysates. The results indicated that Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles can be considered as an excellent purification material. β-glucosidase was immobilized on the surface of Fe 3 O 4 /PMG/IDA-Ni 2+ to form Fe 3 O 4 /PMG/IDA-β-glucosidase by means of covalent bound with imidazolyl and Ni 2+ . The immobilized β-glucosidase exhibited excellent catalytic activity and stabilities compared with free β-glucosidase. In addition, immobilized β-glucosidase can be recycled for many times and retain more than 65% of the original activity. The materials display enormous potential in the aspect of purifying and immobilizing enzyme.
研究领域:
生物化学
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生物技术
·
酶学
·
纳米技术
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2022
·
IF 7.139
Fan Yang,Bo Yang,Karuppasamy Kohila Rani,Yuhui Wei,Xinglan Peng,Limin Wang,Xiaotian Liu,Du-Hong Chen,Youjun Fan,Wei Chen
Carbon-based materials have been often employed as electrocatalytic substrates because of their large surface area/highly porous structure . Similar to carbon substrates, the non-carbon related materials such as transition metals also play an important role in improving catalytic performance. However, the simultaneous synthesis and metallic functionalization of carbon substrates is a highly challenging issue. Herein, a hydrothermal method has been used for the preparation of Ni-functionalized porous carbon balls. The significant role of Ni 2+ ions in the synthesis of porous carbon balls has been confirmed. The results of transmission electron microscopy indicate that, the as-prepared porous carbon balls were suitable for the dispersion of Pt nanoparticles with small particle size (less than 4 nm). In addition to providing the OH ads species, the Ni can also modify the surface electronic structure of Pt. Electrochemical measurements results reveal that, under the strong interactions between Ni and Pt, the as-prepared porous carbon balls supported Pt nanoparticles (Pt/Ni-CB) catalyst possesses excellent electrocatalytic activity , stability and CO anti-poisoning capability towards methanol electrooxidation reaction (MOR). This work opens a novel idea for the construction of the metal functionalization of carbon substrates and their subsequent applications in other electrocatalytic reactions .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Mianxing Luo,Meng Zhang,Changbiao Chi,Guo Chen
It is of great significance to study the effect of multi-enzyme aggregation behavior at the interface on the formation of multi-enzyme complexes and their co-catalytic characteristics, which is helpful for us to design and construct immobilized multi-enzyme complex systems for in vitro synthetic biology. Here, a magnetic microsphere with chelated Ni 2+ , was prepared to explore the self-assembly characteristics of PduQ-SpyTag (P-T) and Nox-SpyCatcher (N C) on its surface, based on the mixed interaction mode consisting the affinity of His-tag/Ni 2+ and covalent binding of SpyTag/SpyCatcher. After studying the effect of saturated or unsaturated adsorption of P-T on the covalent binding between P-T and N C at the interface, a possible multienzyme interaction mechanism for the affinity-assisted covalent self-assembly on the Ni 2+ chelating surface was proposed. The time evolution of NADH showed that the immobilized P-T/N-C complex formed by this method and the free P-T/N-C complex exhibited similar synergistic catalytic properties, and presented higher catalytic efficiency than the simple mixing of P-T and N C. The optimal catalytic conditions, stability and reusability of the immobilized multi-enzyme complexes prepared in this study were also discussed by comparing them with free enzymes. In this study, we demonstrate a simple and effective strategy for self-assembling SpyTag/SpyCatcher fusion proteins on the surface of magnetic beads, which is inspiring for the construction of more cascade enzyme systems at the interface. It provides a new method for facilitating the rapid construction of immobilized multi-enzyme complexes in vitro from the crude cell lysis .
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PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Haoyu Yue,Zhijie Shang,Pan Xu,Dongyue Feng,Xinxue Li
A novel EDTA functionalized chitooligosaccharide/sodium alginate/Ca 2+ physical double network hydrogel (ECSDNH) was prepared by using of high-salinity oilfield produced water. The formation and structure of ECSDNH were characterized by FT-IR and SEM. Various factors, including EDTA functionalized chitooligosaccharide content, pH of solution, initial concentration of heavy metal ions, temperature and contact time, were thoroughly investigated to explore their effect on adsorption capacity of ECSDNH. The obtained results showed that ECSDNH had higher adsorption capacity for Zn 2+ , Ni 2+ and Mn 2+ . The maximum adsorption capacities of Zn 2+ , Ni 2+ and Mn 2+ were 160.19 mg/g, 72.67 mg/g and 52.43 mg/g at 298 K, respectively. The pseudo-second-order kinetic model and Langmuir isotherm model fit the experimental data fairly well. The thermodynamic parameters of the adsorption, Δ H ° , Δ S ° , and Δ G ° , were also calculated. In addition, the ECSDNH still had higher removal efficiency for three metal ions after three adsorption-regeneration cycles, indicating its potential application for removing heavy metal ions from wastewater.
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CERAMICS INTERNATIONAL
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2025
·
IF 5.6
Tong Feng,Lijia Liang,Hui Zhou,Yuanlin Jin,Zhangwen Long,Dacheng Zhou,Xiaqing Jiang,Bitao Liu,Jianbei Qiu
The rapid advancement of near-infrared light source technology has positioned phosphor-converted light-emitting diodes (PC-LEDs) operating within the near-infrared spectrum at the forefront of interdisciplinary research, with extensive applications in biomedicine, food quality assessment, and agricultural monitoring. However, the development of light sources that cover the second near-infrared spectral window (NIR-II, 1000–1700 nm) continues to encounter significant technical challenges. Inspired by chemical unit co-substitution strategy, we successfully developed a broadband NIR-II phosphor designated as (Li 0.7 Mg 0.3 )(Ga 0.7 Mg 0.3 )TiO 4 :0.003Ni 2+ ((L 0.7 M 0.3 )(G 0.7 M 0.3 )T:0.003Ni 2+ ) through [Mg 2+ -Mg 2+ ] dual-site substitution in orthorhombic LiGaTiO 4 (LGT) normal spinel. This co-substitution strategy resulted in an impressive enhancement of the internal quantum efficiency by 29.77 %–64.8 %, while maintaining thermal stability without significant attenuation at elevated temperature. To validate its practical utility, we fabricated prototype for a NIR phosphor-converted LED by integrating (L 0.7 M 0.3 )(G 0.7 M 0.3 )T:0.003Ni 2+ a commercial 340 nm chip. The resulting device demonstrates promising potential for applications in food safety inspection and deep-tissue infrared imaging. This work provides a perspective for designing broadband high-efficiency NIR-II phosphors via modulation of crystal field environment.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2025
·
IF 8.3
Liye Shuai,Qun Li,Jia Liu,Jiabin Wu,Xinning Huang,Jianglong Yu,Jinxiao Dou,Huaiguang Li,Xingxing Chen
The rational design of efficient photoanodes is essential for advancing solar-driven water oxidation. This study addresses the intrinsic challenges of bismuth vanadate (BiVO 4 ), such as sluggish charge kinetics and severe surface recombination, through the construction of a ternary composite photoanode via synergistic interfacial modification and cocatalyst integration. A hierarchical architecture was fabricated through sequential low-temperature chemical bath deposition and electrodeposition, integrating a CoFe-layered double hydroxide (CoFe-LDH) interlayer and nickel-hydroxynitrate (Ni 2 (NO 3 ) 2 (OH) 2 ) onto BiVO 4 substrates. The optimized BiVO 4 /CoFe-LDH@Ni 2 (NO 3 ) 2 (OH) 2 photoanode achieves a photocurrent density of 5.57 mA/cm 2 at 1.23 V vs. reversible hydrogen electrode (V RHE ) under Air Mass 1.5 Global (AM 1.5G) illumination, accompanied by a 251 mV cathodic shift in onset potential. Comprehensive characterizations-including X-ray photoelectron spectroscopy (XPS), open-circuit photovoltage (OCP) decay, time-resolved photoluminescence (TRPL) decay, electron paramagnetic resonance (EPR) and Kelvin probe force microscopy (KPFM)-reveal that the oxygen vacancy (O v )-rich CoFe-LDH@Ni 2 (NO 3 ) 2 (OH) 2 layer facilitates efficient charge transfer within BiVO 4 and generates a pronounced built-in electric field, thereby significantly enhancing interfacial charge separation. Concurrently, density functional theory (DFT) calculations demonstrate that the interfacial effect of CoFe-LDH@Ni 2 (NO 3 ) 2 (OH) 2 substantially lowers the thermodynamic barrier for the oxygen evolution reaction (OER). This study offers fundamental insights for fabricating high-efficiency BiVO 4 -based photoanodes.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2020
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IF 3.99
Weihua Meng,Hongjuan Wu,Ruifang Wu,Tian Wang,Aiqing Wang,Jing Ma,Jianzhong Xu,Hongqiang Qu
A novel magnesium hydroxide-polyphosphazene-Ni 2+ (MH-PZPN-Ni) was synthesized by the layer-by-layer assembly strategy and Ni 2+ chelation method. Post modification, the composite was incorporated into ethylene-vinyl acetate (EVA). The morphology, composition and structure of the MH-PZPN-Ni composites were comprehensively characterized by scanning electron microscopy, transmission electron microscope, Fourier transform infrared, X-ray diffraction and X-ray photoelectron spectroscopy. MH-PZPN-Ni could efficiently increase the flame retardancy and smoke suppression of EVA. The MH-PZPN-Ni (60 wt %)-incorporated EVA composite showed a limiting oxygen index value of 30.4 % and was rated as a UL 94 V-0 material. Cone calorimeter test results showed that the peak heat release rate and total heat release of EVA/MH-PZPN-Ni were decreased by 85.8 % and 56.7 %, respectively when compared to pure EVA. The morphology, structure, and chemical composition of the char residue and the pyrolysis products formed during the decomposition of the EVA composites were analyzed to investigate the flame-retardant mechanism of the modified EVA matrix. The production of ammonia (during the process of PZPN-Ni decay) and the water vapor formed due to the combustion of the H· and OH· radicals were found to dilute the concentration of the generated combustible gases. The denser char layer effectively blocked the heat and mass transfer. At the same time, the use of the Ni catalyst resulted in a significantly low production of various volatile products (such as CO and aliphatic and aromatic compounds). Moreover, PZPN-Ni enhanced the mechanical properties of EVA. The elongation at break increased by 74.9 %.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2024
·
IF 4.1
Yuxiang Jiao,Yifei Li,Huaijun Tang,Shanglan Xian,Yonghui He,Long Wang,Jinghao Zhang
Herein, a cationic iridium(III) complex [(ppy) 2 Ir(IPP)]PF 6 (ppy: 2-phenylpyridine; IPP: 2-(1 H -imidazo[4,5- f ][1,10]phenanthrolin-2-yl)phenol) was synthesized. Under the excitation of 328 nm (λ ex = 328 nm) UV light, this complex emitted bright yellow orange light (500 ∼ 700 nm, λ em,max = 582 nm), the Stokes shift was up to 254 nm, mainly due to excited-state intramolecular proton transfer (ESIPT) occurring in its ancillary ligand IPP. [(ppy) 2 Ir(IPP)]PF 6 was very sensitive to Ni 2+ , which was caused by the ESIPT being hindered by the coordination of [(ppy) 2 Ir(IPP)]PF 6 with Ni 2+ . Its bright yellow orange light disappeared within 1 min when Ni 2+ (1.0 equiv.) was added in its solution (in DMSO/H 2 O, v/v = 999/1), therefore, it can be used for “turn-off” chemiluminescent detection of Ni 2+ . The recognition of [(ppy) 2 Ir(IPP)]PF 6 to Ni 2+ was not interfered by many common metal ions (such as Na + , K + , Li + , Mg 2+ , Al 3+ , Ca 2+ , Fe 3+ , Ag + , Zn 2+ , Hg 2+ , Co 2+ , Pb 2+ , Mn 2+ , Ba 2+ or Cr 3+ ), however, Cu 2+ was an interfering metal ion, but its interference can be completely masked by thiourea. The detection limit was as low as 3.31 × 10 −7 mol∙L -1 (0.331 μM). The Job’s plot analysis revealed that the stoichiometry of [(ppy) 2 Ir(IPP)]PF 6 with Ni 2+ was 1:1, its detecting mechanism based on ESIPT effect was demonstrated by 1 H NMR spectra and density functional theory (DFT) calculation.
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