INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2017
·
IF 2.962
Guang Guo,Fang Tian,Keqiang Ding,Lihong Wang,Tingfeng Liu,Feng Yang
The metabolism of polycyclic aromatic hydrocarbons (PAHs) in polluted soil is limited by low contaminant bioaccessibility and low degradation by bacteria. A bacterial consortium that degraded 76 per cent of pyrene within 10 days was enriched from a contaminated soil. Hydroxypropyl-β-cyclodextrin (HPCD) enhanced the rate of degradation by the consortium. The bacterial consortium was used to bioremediate PAH-contaminated soil (pyrene and benzo[α]pyrene) in the presence or absence of HPCD. Enhanced PAH mineralization was observed after 10 weeks, particularly in the presence of HPCD, which increased the bioavailability of PAHs. Illumina sequencing was performed to analyze the changes in the bacterial communities. The dominant genus was Burkholderia in all treated soils. Importantly, the inoculated bacteria cannot compete with native bacteria and will therefore not invade the community. The significantly stimulated genera were Burkholderia , Nitratireductor , Nevskia and Sulfuritalea . These results suggest that the combination of bacteria with HPCD can enhance the bioremediation of PAH-contaminated soil.
研究领域:
环境科学
·
微生物学
·
土壤学
·
生物修复
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·841
·
IF 5.3
Zhao Yue-Dan,Chen Ke-Yan,Xia Lei,Jin Chen-Yu,Zhao Jun-Jian,Li Hai-Jie,Xu Hou-Jun,Wang Man-Man
Mixed-ligand metal organic frameworks have received great attentions as novel sorbents for sample preparation due to their fascinating structures and intriguing functionalities. Here, we report the fabrication of mixed-linker ZIF-8-90 s with tunable properties for effervescent tablet-assisted dispersive solid-phase extraction (ET-DSPE) of polycyclic aromatic hydrocarbons (PAHs) in environmental water before HPLC separation and hydroxyl metabolites of PAHs (OH-PAHs) in urine samples prior to LC-MS/MS. ZIF-8-90 s based effervescent tablets possessed the high affinity towards analytes via Zn-O bond and π-π interaction, and afforded a great excluding ability to interfering components from urine samples. ET-DSPE of PAHs from 15 mL water sample with ZIF-8 25 -90 75 gave enhancement factors of 122–155, linear ranges of 4-10 5 ng/L, detection limits ( S/N = 3) of 1–8 ng/L, and recoveries of 81.1%-103%. For the determination of OH-PAHs, the proposed ET-DSPE method using ZIF-8 85 -90 15 allowed direct loading of 4 mL of crude human urine sample and provided the linearity range of 0.004-100 ng/mL, detection limits of 0.001–0.008 ng/mL, and recoveries of 82.1%-104%. In conclusion, ZIF-8-90-based ET-DSPE coupled with HPLC and LC-MS/MS were successfully applied to monitor both PAHs in environmental water and OH-PAHs in urine in a facile, efficient and reliable way. Graphical
研究领域:
分析化学
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环境科学
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毒理学
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暴露评估
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材料科学
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样品前处理
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Xian-xian Chen,Chao Li,Krishna Kumar Selvaraj,Qing-song Ji,Zhi-hong Fang,Shao-gui Yang,Shi-yin Li,Li-min Zhang,Huan He
In this study, the relative bioavailability (RBA) of nitrated polycyclic aromatic hydrocarbons (NPAHs) in soil samples ( n = 30) was assessed using an in vivo mouse model. Based on the correlation between the bioaccessibility data obtained from the Tenax improved traditional Fed ORganic Estimation human Simulation Test (FOREhST) in vitro method (TITF) and the bioavailability data obtained from in vivo experiments, the TITF method was further optimized and simplified by referring to the “Pharmacopoeia of the People's Republic of China: Volume IV, 2020” to adjust the formulation and parameters of the gastrointestinal fluid (GIF) in order to establish a simpler and lower cost in vitro method for the determination of the bioaccessibilities of NPAHs. The dose-accumulation relationship of the in vivo experiment showed that the linear dose-response was better in adipose tissue (R 2 = 0.77–0.93), and the accumulation of NPAHs in adipose tissue was higher than that in kidney or liver tissue. Depending on the mouse adipose model, the NPAHs-RBA ranged from 1.88 % to 73.92 %, and a strongly significant negative relationship (R 2 = 0.94, p < 0.05) was found between the NPAHs-RBA and Log K ow . The simplified experiment of the TITF showed that the composition of the GIF medium had a significant effect on the bioaccessibilities of NPAHs. The NPAH bioaccessibilities measured by the Tenax improved simplified FOREhST method (TISF) (9.0–36.5 %) were higher than that of the traditional FOREhST method (6.8–22.8 %) but significantly lower than that of the TITF method (16.8–55.2 %). With an increase in the bile concentration in the GIF (from 6 to 10 g/L), the bioaccessibilities of NPAHs increased from 9.0 to 36.5 % to 12.9–42.4 %. The accuracies of the four in vitro methods for predicting the bioavailabilities of NPAHs was in the following order: Tenax improved simplified FOREhST method with increased bile concentration (TITF-IB) (R 2 = 0.54–0.87) ≈ TITF (R 2 = 0.55–0.85) > TISF (R 2 = 0.41–0.77) > FOREhST (R 2 = 0.02–0.68). These results indicated that the simple in vitro method could also effectively predict the bioavailabilities of NPAHs.
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xia Yan,Hang Zhao,Hongyan Song,Jun Ma,Xiaofeng Shi
Surface-enhanced Raman scattering (SERS) has great potential for the detection of marine pollutants, but it is still restricted in ultra-trace and quantitative analysis. Here, a strategy for the detection of polycyclic aromatic hydrocarbons (PAHs) was proposed based on Au nanoscale convex polyhedrons (Au NCPs) coated with high-energy facets and embedded with 4-ATP as a probe molecule. Au NCPs acted as SERS substrates and led to limits of detection (LODs) for six common PAHs that reached 0.01 nM. Using internal calibration, the relative standard deviations (RSD) of the spectral stability and reproducibility were as low as 3.36% and 5.11%, respectively. The maximum mean relative errors (AREs) of the predicted and true values were 6.28%. The results indicate that the resulting Au NCPs improved the ultra-trace and quantitative detection of SERS, thus suggesting that the Au NCPs have practical application value in SERS.
研究领域:
环境科学
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分析化学
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纳米技术
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材料科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Changfei Ma,Qun Zhang,Jing Liang,Shan Yang,Tianlong Zhang,Fangqi Ruan,Hongsheng Tang,Hua Li
Polycyclic aromatic hydrocarbons (PAHs) present in oily sludge generated by the petroleum and petrochemical industries have emerged as a prominent concern within the realm of environmental conservation. The precise determination of PAHs holds immense significance in both petroleum geochemistry and environmental protection. In this study, a combination of surface-enhanced Raman spectroscopy (SERS) and solid–liquid extraction was employed for the screening of PAHs in oily sludge. Methanol was utilized as the extraction solvent for PAHs, while nanosilver-silicon coupling substrates were employed for their detection. The SERS spectrum was acquired using a portable Raman spectrometer. The nano silver-silicon coupling substrate exhibits excellent uniformity, with relative standard deviations (RSDs) of Phenanthrene, Fluoranthrene, Fluorene and Naphthalene (Phe, Flt, Flu and Nap) being 2.8%, 1.08%, 1.41%, and 5.44% respectively. Moreover, the limits of detection (LODs) achieved remarkable values of 0.542 μg/g, 0.342 μg/g, 0.541 μg/g, and 5.132 μg/g. The quantitative analysis of PAHs in oily sludge was investigated using SERS technology combined with partial least squares (PLS). The optimal PLS calibration model was optimized by combining spectral preprocessing methods and using the SiPLS (Synergy interval partial least squares)-VIP (Variable Importance in Projection) hybrid variable selection strategy. The prediction performance of the D1st (First derivative)-WT (Wavelet transform)-SiPLS-VIP-PLS model was deemed satisfactory, as evidenced by high R 2 P values of 0.9851, 0.9917, and 0.9925 for Phe, Flt, and Flu respectively; additionally, the corresponding MREP values were found to be 0.0580, 0.0668, and 0.0669 respectively. However, for Nap analysis, the D1st-WT-PLS model proved to be a better calibration model with an R 2 P value of 0.9864 and an MREP (Mean relative error of prediction) value of 0.0713. In summary, SERS technology combined with PLS based on different spectral pretreatment methods and mixed variable selection strategies is a promising method for quantitative analysis of PAHs in oily sludge, which will provide new ideas and methods for the quantitative analysis of PAHs in oily sludge.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
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IF 3.8
Yiran Xia,Lushuai Wang,Yang Liu,Jiawei Liu,Quan Bai
Covalent organic frameworks (COFs) show promise as a stationary phase in high performance liquid chromatography (HPLC). However, there are only a few COFs-based stationary phases developed for HPLC separation so far. Therefore, it is crucial to not only develop more varieties of COFs-type stationary phases for HPLC separation, but also to explore the retention mechanism of solutes on these stationary phases. In this paper, a new in-situ growth method was developed to prepare β -ketoenamine COF-TpPa-1@SiO 2 composite microspheres, using spherical silica as the core material and COF-TpPa-1 fabricated by covalent conjugation of 1,3,5-triformylphloroglucinol (Tp) and p -phenylenediamine (Pa-1) as the COF shells. The resulting microspheres exhibit uniform morphology, good monodispersity, large specific surface area, narrow size distribution, and high stability. Due to diverse functional groups in the structure of COF-TpPa-1, the microspheres can offer multiple interactions, such as hydrophobic, π-π stacking and electron-donor–acceptor (EDA) between COFs and analytes. As a result, the COF-TpPa-1@SiO 2 composite microspheres can be used as a mixed-mode stationary phase for HPLC separation. The chromatographic performance and retention mechanism of the COF-TpPa-1@SiO 2 packed column were investigated by separating polar and non-polar solutes, as well as isomers, in various HPLC modes, including reversed-phase liquid chromatography (RPLC), hydrophilic interaction chromatography (HILIC), and RPLC/HILIC mixed-mode chromatography. The results showed successful separation of non-polar alkylbenzene homologues, polycyclic aromatic hydrocarbons (PAHs), and polar amines and phenols in RPLC mode. The “U-shaped” curves of retention factor with the ACN concentration in mobile phase for four nucleobases indicated that the solute retention on the column followed a mixed mode mechanism of RPLC/HILIC. Compared to a traditional C18 column, the COF-TpPa-1@SiO 2 column exhibited superior separation efficiency, stability, repeatability and reproducibility in the separation of analytes with different polarities. The column enhanced the aromatic, shape and planar selectivity for PAHs and isomers through π-π interaction and improved the separation efficiency for electron-deficient compounds due to EDA effect. At last, the column was successfully used to separate and detect the residues of 5 phenylurea herbicides (PUHs) in soil. All these results indicate the potential of COFs for chromatography applications.
研究领域:
分析化学
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材料科学
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色谱学
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DOI
PubMed
高影响因子
eFood
·
2024
·Vol. 5
·期 2
·e143
·
IF 4
Xiaomei Lv,Haolin Zhang,Qiuyi Lin,Ruiwei Xie,Bing-Huei Chen,Yu-Wen Lai,Hui Teng,Lei Chen,Hui Cao
Polycyclic aromatic hydrocarbons (PAHs) are found in many common foods and increase the risk of cancer in humans. This study systematically screened the components that may be related to the formation of PAHs by an excessive addition of 19 amino acids and glucose in the beef patties. Phenylalanine and glucose exhibited the potential as promoters of PAHs, while lysine, aspartic acid, glutamic acid, valine, and methionine exhibited excellent potential as inhibitors of PAHs. Subsequently, a chemical model containing phenylalanine and glucose was established to confirm their roles as precursors of PAHs. The formation mechanism of PAHs showed that naphthalene was first formed as a basic structure, and then, PAHs with more rings were further formed through substitution, addition reactions, or intramolecular cyclization reactions. The mitigative effects of four flavonoids on total PAH contents were in the order: kaempferol > naringenin (better for individual PAH) > myricetin > quercetin (contradictory), which suggested that the antioxidant ability or radical scavenging capacity of flavonoids may be one of the pathways for PAH inhibition.
研究领域:
食品科学
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化学
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营养学
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毒理学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 185
·期 1
·1-10
·
IF 4.58
Wang Wenchang,Li Zhi,Wang Wenjin,Zhang Lihong,Zhang Shuaihua,Wang Chun,Wang Zhi
A porous aromatic framework of type PAF-6 was synthesized and explored as a coating onto a steel wire for using in solid-phase microextraction of polycyclic aromatic hydrocarbons (PAHs), phthalate plasticizers, and n -alkanes. The extraction temperature, extraction time, salt concentration, agitation speed, desorption temperature, and desorption time were optimized. This method for SPME resulted in the enrichment factors ranging from 122 to 1090 for PAHs (naphthalene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene), from 122 to 271 for plasticizers (diisobutyl phthalate, dibutyl phthalate, benzyl butyl phthalate, dicyclohexyl phthalate), and from 9 to 113 for n -alkanes ( n -undecane, n -dodecane, n -tridecane, n -tetradecane, n -pentadecane, n -hexadecane, n -octadecane and n -eicosane). The good extraction of the PAHs is assumed to be due to their π-stacking interaction and hydrophobic effect. The PAF-6 coated fibers are durable and can be reused more than 100 times without significant loss of extraction performance. In combination with GC-MS detection, the method has limits of detection in the range from 0.8 to 4.2 ng L −1 in case of PAHs. The relative standard deviations for five replicate determinations of the PAHs by using one fiber are in the range from 5.2 to 8.5%. When using different fibers, they range from 7.1 to 9.6%. The recoveries of PAHs from water samples at a spiking level of 20 ng L −1 are in the range from 89.5 to 103.1%, with relative standard deviations ranging from 4.0 to 9.3%.
A porous aromatic framework of type PAF-6 was synthesized and used as a novel coating for the solid-phase microextraction of polycyclic aromatic hydrocarbons prior to their determination by gas chromatography with mass spectrometric detection.
研究领域:
分析化学
·
环境科学
·
材料科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）