JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Lan Qiu,Xiaoyuan Guo,Hojae Shim,Tianwei Hao,Zhiwei Liang,Shanquan Wang,Zhenmei Lu,Qihong Lu,Zhili He
The polychlorinated aromatic antimicrobial agent triclosan (TCS) is widely used to indiscriminately and rapidly kill microorganisms. The global use of TCS has led to widespread environmental contamination, posing significant threats to ecosystem and human health. Here we reported a newly isolated Pseudomonas sp. W03 for degrading TCS metabolically at concentrations up to 10 mg/L. This strain exhibited optimal degradation activity at 30°C and pH 7.0, and retained substantial activity at pH 4.0, although it was sensitive to alkaline conditions. Genomic analysis of strain W03 revealed a circular chromosome comprising 6075,907 bp with a GC content of 65.08 %. A novel TCS degradation pathway, involving dechlorination, oxidation, ether bond fission, and reoxidation processes, was identified. Also, the study mapped the global distribution of analogous Pseudomonas using 16S rRNA gene sequences, revealing their widespread presence in diverse aquatic environments, with a significant abundance in wastewater systems. These findings indicated that these bacteria play a critical ecological role in both natural and engineered environments, particularly in the degradation of organic pollutants. This study enhances our understanding of microbial degradation of emerging contaminants and presents a promising candidate for bioremediation strategies aimed at mitigating TCS-related water pollution.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jinle Ma,Jianan Liu,Junya Zhu,Hongman Hou,Jingran Bi,Hongshun Hao,Gongliang Zhang
In this study, the synergistic antimicrobial effect of allyl isothiocyanate (AITC) and cinnamaldehyde (CAL) against Pseudomonas fluorescens ( P. fluorescens ) was investigated. The fractional inhibitory concentration index (FICI) value of 0.5 in the combination of AITC and CAL indicated the synergistic effect on P. fluorescens by reducing their dosages. Compared with the individual AITC and CAL, the combined treatment group increased protein leakage concentration and intracellular reactive oxygen species content, as well as significantly inhibited the motility of P. fluorescens ( P < 0.05), with a reduction of 6.3 and 10.3 mm in swimming diameter, respectively. Meanwhile, this combination resulted in a 93.4 % reduction in biofilm formation compared to the untreated group. Moreover, combined treatment with AITC and CAL significantly inhibited the growth of P. fluorescens and delayed the increase of total volatile base nitrogen in chilled beef. Therefore, this study provides a reference for the application of a combination of AITC and CAL for beef preservation.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yi-Ming Zhao,Lin Zhang,Yulong Bao,Yiting Guo,Haile Ma,Ronghai He,Paula Bourke,Da-Wen Sun
The use of plasma-activated water (PAW) to inactivate microorganisms in the food industry has attracted wide attention, while the research of PAW as a quorum sensing inhibitor (QSI) to mitigate biofilm is quite limited, and the molecular mechanisms have not been reported. In this study, PAW treatments under sub-inhibitory conditions of Pseudomonas fluorescens were selected, and no inhibitory effects on bacterial viable counts or growth curves were observed. Whereas biofilm biomass was significantly reduced by 28.38 % to 43.53 % during the 12 to 72 h incubation after PAW-60 treatment ( P < 0.05). Extracellular polymeric substances (EPS) production was decreased, with a maximal reduction of 57.38 % in extracellular proteins, 53.43 % in polysaccharides, and 86.71 % in extracellular DNA compared to the control. Microscopic analyses evidenced that the biofilm became looser and thinner after PAW treatment. The surface properties of P. fluorescens , including hydrophobicity, auto-aggregation, and surface potential, were significantly declined by 31.15 %, 25.67 %, and 47.3 % respectively, after PAW-60 treatment at 12 h. Both swarming and swimming abilities of P. fluorescens were completely repressed by PAW-60 at 12 h. Moreover, PAW-60 significantly reduced QS signaling molecules production of AHLs and AI-2 by 46.98 % and 35.72 %, respectively ( P < 0.05). Transcriptomic analysis and RT-qPCR demonstrated that PAW-60 suppressed chemotaxis and flagella assembly gene expression levels and downregulated genes related to the QS pathway, including the synthesis ( FadD1 ) and receptor ( LuxR ) of AHLs, and transporter protein genes of AI-2 ( RbsA , RbsC ). This study revealed the molecular mechanisms of PAW inhibition on biofilm formation of P. fluorescens by interfering with the QS pathway.
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MicrobiologyOpen
·
2018
·Vol. 8
·期 5
·e00720
·
IF 2.682
Pengfei She,Zhen Luo,Lihua Chen,Yong Wu
Graphical OFLX showed significant inhibitory effects on Pseudomonas aeruginosa biofilm formation in a concentration-dependent manner. RNA-seq revealed that the differentially expressed genes mainly related to metabolic, secondary metabolites, and quorum sensing biosynthesis when treated with OFLX. Clinical isolates from patients with respiratory tract infections were studied.
\ Microbial biofilms are formed in a variety of clinical situations and increase antibiotic resistance of the pathogen by almost ~1,000 times. The effect of levofloxacin (OFLX) on the biofilms of Pseudomonas aeruginosa strain PAO1 and the clinical isolates was investigated by crystal violet staining and confocal laser scanning microscope. The transcriptional alteration in the PAO1 biofilms upon OFLX treatment was also analyzed by RNA sequencing (RNA- seq ). We found that while OFLX significantly inhibited P. aeruginosa biofilm formation in a dose-dependent manner, it could not completely eradicate preformed biofilms even at higher concentrations. RNA- seq revealed that PAO1 genes related to metabolism, formation of secondary metabolites, and quorum sensing biosynthesis were differentially expressed in the biofilms treated with OFLX. Our data might be useful in determining the optimum OFLX concentration needed for P. aeruginosa biofilm inhibition and eradication in patients with respiratory tract infections.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2025
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IF 1.3
Wei Tian,Longhui Luo,Bingce Wang,Zhibo Zhao,Chao Kang,Dongmei Chen,Xiufang Yan,Youhua Long
Pseudomonas syringae pv. actinidiae (Psa), the pathogen that causes bacterial canker disease in kiwifruit, has brought about substantial losses to the kiwifruit industry. Investigating the infection process at both the cellular and microscopic levels is of great significance for the formulation of effective control strategies against this disease. Thus, the establishment of appropriate chemical imaging analysis methods becomes essential. Confocal Raman microspectral imaging (CRMI), combined with chemometrics, provides an intuitive means to visualize and characterize the spatiotemporal changes of biopolymers in the cell walls of both healthy and infected kiwifruit stems. Raman spectra of different infected stems exhibit clustering effects in Principal component analysis (PCA), and a classification model constructed using Support vector machines (SVM) achieves an accuracy of 97%. Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) is utilized to resolve spectral matrices and concentration profiles from raw Raman imaging signals. The reconstructed concentration data yields accurate molecular imaging maps of High-methylated pectin (HMP), Low-methylated pectin (LMP), cellulose, hemicellulose, and lignin. The results indicate that, three days after Psa infection, the content of cellulose and HMP in the cell wall increases, while the changes in hemicellulose, lignin, and LMP are minimal. However, five days after infection, the contents of HMP, LMP, cellulose, hemicellulose, and lignin decrease significantly, resulting in the disruption of the cell-wall structure. The chemical imaging method proposed in this study shows great promise as an effective means for studying the bacterial infection process in kiwifruit stems at the cellular level.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2025
·
IF 5.2
Yongqi Tian,Yuanyuan Chen,Ruyang Huang,Tao Wang,Shaoyun Wang
Synergistic bacteriostatic action represents a potent strategy for combating microbial contamination in the food industry. This study investigated the synergistic bacteriostatic effect of epigallocatechin gallate (EGCG) and indole-3-carboxaldehyde (I3A). Results showed a pronounced synergistic action of EGCG and I3A against diverse food spoilage microorganisms, most notably Pseudomonas aeruginosa ( P. aeruginosa ), with a fractional inhibitory concentration index (FICI) of 0.25. Further research revealed that EGCG disrupted the cell wall and cell membrane of P. aeruginosa , while supplementing I3A significantly boosted the concentration of intracellular reactive oxygen species, thereby inflicting cellular damage. Moreover, the EGCG-I3A treatment inhibited the biofilm formation of P. aeruginosa in a dose-dependent manner, with the effectiveness increasing with the quantity of I3A added. Metabolomic study revealed a perturbation in glutathione and taurine metabolic pathways post synergistic treatment, compromising redox homeostasis. This synergistic treatment also downregulated uracil, proline, and glutamate metabolites, thereby suppressing Quorum Sensing (QS) and biofilm-associated expression within P. aeruginosa . Additionally, the combination significantly inhibited P. aeruginosa growth in fish meat. In essence, this study underscored the synergistic bacteriostatic efficacy of EGCG and I3A, highlighting its potential application in food preservation.
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Cell Chemical Biology
·
2020
·
IF 7.739
Feng Xie,Shengwang Dai,Yu Zhao,Pei Huang,Shen Yu,Biao Ren,Qiushui Wang,Zengchun Ji,Gil Alterovitz,Qi Zhang,Jingyu Zhang,Xiangyin Chen,Lan Jiang,Fuhang Song,Hongwei Liu,Frederick M. Ausubel,Xueting Liu,Huanqin Dai,Lixin Zhang
Summary Pioneering microbial genomic surveys have revealed numerous untapped biosynthetic gene clusters, unveiling the great potential of new natural products. Here, using a combination of genome mining, mutasynthesis, and activity screening in an infection model comprising Caenorhabditis elegans and Pseudomonas aeruginosa , we identified candidate virulence-blocking amychelin siderophore compounds from actinomycetes. Subsequently, we developed unreported analogs of these virulence-blocking siderophores with improved potency by exploiting an Amycolatopsis methanolica strain 239 T chorismate to salicylate a biosynthetic subpathway for mutasynthesis. This allowed us to generate the fluorinated amychelin, fluoroamychelin I, which rescued C . elegans from P . aeruginosa -mediated killing with an EC 50 value of 1.4 μM, outperforming traditional antibiotics including ceftazidime and meropenem. In general, this paper describes an efficient platform for the identification and production of classes of anti-microbial compounds with potential unique modes of action.
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JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
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2022
·
IF 2.582
Zaihui Huang,Zheng Qi,Xiaohu Ding,Chunguang Liu
This study investigated the influence of N-chlorosuccinimide (NCS) pretreatment on the antimicrobial effect of benzalkonium chloride (BZC, representative of QACs) against biofilm bacteria and its mechanisms. Results show that 0.04 − 0.07 mmol/L NCS pretreatment significantly increased the antimicrobial efficacy of 0.03 mmol/L BZC on biofilm cells by 30% − 70%. The main mechanisms involved membrane permeability, oxidative damage, and metabolic disorder. More precisely, NCS pretreatment increased the permeability of bacteria and reduced the activity of the electron transport system (ETS) and dehydrogenase (DHA). At the same time, the oxidative damage of both endogenous and exogenous ROS and the disorder of the antioxidant enzymes (superoxide dismutase and catalase) further improved their combined antibacterial ability. Moreover, NCS pretreatment greatly reduced the resistance of biofilm Pseudomonas aeruginosa to BZC. The findings of the study provide a new method to effectively enhance the antimicrobial efficiency of quaternary ammonium cationic surfactants (e.g., BZC) and reduce bacterial resistance, as well as a scientific guidance for the development of new antimicrobial products.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Wenbin Guo,Zhengguang Shi,Xue Yu,Jingjing Duan
An important environmental issue is the pollution caused by poly(ethylene terephthalate) (PET) and the emitted monomers terephthalic acid (TPA) and ethylene glycol (EG). The TPA biodegradation pathway has been well documented for the microbial biodegradation and assimilation of PET; however, the PET biodegradation process by marine microorganisms via the EG biodegradation pathway is less understood. Here, we demonstrate how the marine bacteria Pseudomonas chengduensis BC1815’s membrane-anchored PET esterase (OF113_10420) breaks down PET. We also explain the characteristics of this enzyme and demonstrate that the strain assimilates PET via the EG biodegradation pathway. In addition to depolymerizing PET, PET esterase (OF113_10420) hydrolyzes BHET and MHET to generate EG more efficiently in alkaline circumstances. It has been discovered that P. chengduensis BC1815 cells exhibiting surface-displayed PET esterase (OF113_10420) have the potential to function as cell biocatalysts for the breakdown of PET. Additionally, we look into the microbial communities that have PET esterase linked to the EG biodegradation pathway, primarily those belonging to the phyla Proteobacteria and Actinobacteriota. Consequently, research on the microbial absorption and biodegradation of PET via the EG metabolism route increases our knowledge of the bioremediation of PET pollution in the marine environment and contributes to our understanding of the environmental destiny of PET pollutants in the marine environment.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Wei Ge,Linxuan Zhang,Fanli Meng,Chengming Tian
Poplar anthracnose caused by Colletotrichum gloeosporioides is one of the major forest diseases worldwide, causing incalculable losses in forestry production every year. In this study, an endophytic bacterium with significant antagonistic activity against C. gloeosporioides was isolated from Populus canadensis leaves, identified and named Pseudomonas atacamensis GZ-3. The specific biological functions of strain GZ-3 were analyzed using whole genome sequencing. The volatile organic compounds (VOCs) produced by strain GZ-3 had good antifungal activity against C. gloeosporioides . These VOCs could effectively inhibit mycelial growth and conidial germination of C. gloeosporioides . In addition, metabolomics analysis revealed that VOCs could help poplar resist infection of C. gloeosporioides by inducing the synthesis of flavonoids in poplar. 3-methyl-1-butanol and 2-heptanone were the main antifungal compounds in VOCs. Therefore, Pseudomonas atacamensis GZ-3 had good potential for biocontrol. This study provided a new strategy for the biological control of poplar anthracnose.
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JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 10
·6083-6094
·
IF 4.22
Liu Mei,Guo Rong,Ma Yue
Polyethyleneimine (PEI) is known for its antibacterial properties and ease of modification. How to improve the specific antibacterial activity of PEI with low toxicity is a research hotspot. In this work, the PEI was used to synthesize a fucose-modified polyethyleneimine copolymer particles (Fuc-PEI CPs) via nucleophilic addition between primary amine and aldehyde groups. Fucose (Fuc) can capture Pseudomonas aeruginosa ( P. aeruginosa ) with LecB, which has a good targeting property toward P. aeruginosa. The Fuc-PEI CPs’ antibacterial activity was investigated by plate counting method. Compared with unmodified PEI, Fuc-PEI CPs (2.5 μg/mL) showed unique bactericidal ability against P. aeruginosa , and less cytotoxicity and excellent biocompatibility. The antimicrobial mechanism indicated that the improvement of PEI antibacterial activity against P. aeruginosa ascribed to the Fuc’s unique recognition of bacteria and PEIs’ ability to destroy the cell membrane of bacteria. The Fuc-PEI CPs as a new antibacterial agent shed light on the constructing a novel antibacterial pathway for killing P. aeruginosa .
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iScience
·
2025
·Vol. 28
·期 12
·
IF 4.1
Yao Di,Wenfei Luan,Lihua Sun,Jing Qi,Hongmei Xia,Fan Li,Niu Zhai
Poly(ε-caprolactone) (PCL) is a widely used synthetic polymer with significant commercial applications, and its degradation and synthesis have become the focus of considerable research. In this study, we purified a PCL-degrading enzyme, PCLase0801, produced by Pseudomonas sp. DS0801. The enzyme was purified to homogeneity and had a molecular weight of 30.4 kDa. It exhibited optimal activity at 40°C and pH 8.0, hydrolyzing PCL into monomers, dimers, and trimers. The enzyme was identified as a lipase and showed good tolerance to organic solvents. Additionally, PCLase0801 catalyzed the ring-opening polymerization of ε-caprolactone, producing PCL with a molecular weight of 6050 g/mol and favorable structural properties. This work provides new insights into the potential applications of PCL-degrading enzymes in PCL treatment and biosynthesis.
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