Regenerative Biomaterials
·
2025
·
IF 8.1
Yang Xiaoling,Li Chenchen,Li Bo,Zhang Yuanyuan,Li Jinping,Liu Na,Nie Xin,Zhang Dawei,Zhou Ming,Liao Xiaoling
Hydrogel-based wound dressings, which facilitate rapid wound closure and healing, are essential for effective wound management. However, the development of an ideal hydrogel that possesses excellent mechanical properties, effective self-healing capabilities, tissue adherence and antimicrobial characteristics for wound dressing presents a significant challenge in clinical settings. Inspired by lotus-fiber and mussel, we synthesized a novel multifunctional hydrogel composed of bacterial cellulose-reinforced dopamine-grafted oxidized hyaluronic acid/polyacrylamide (OHA-DA/PAM/BC). This was achieved through a one-pot reaction that employed free radical polymerization of acrylamide, dynamic Schiff bonding and intermolecular hydrogen bonding. Compared with the pure PAM hydrogels, which exhibited an elongation at break of 4022% and a maximum tensile strength of 26.42 kPa, the OHA-DA/PAM hydrogel demonstrated significantly enhanced stretchability at 9949% and an increased tensile strength of 34.73 kPa when 0.3% OHA-DA was incorporated during hydrogel formulation. Notably, the addition of 0.8% BC significantly enhanced the tensile strength to 57.04 kPa and super-stretchability to 10679%. The OHA-DA/PAM/BC hydrogel also exhibited remarkable self-healing capabilities, achieving a mechanical recovery of 84.74% within 12 hours. Additionally, its adhesive and injectable properties are advantageous for dynamic wound repair. Furthermore, the OHA-DA/PAM/BC hydrogel exhibited minimal hemolytic activity and potent intrinsic antibacterial properties against both E. coli and S. aureus. In a mouse model of wound healing, this hydrogel reduced the healing duration to 14 days while enhancing the regeneration of both skin structure and function. Histological analyses further revealed that the hydrogel significantly promoted the development of well-organized granulation tissue, angiogenic tissue and collagen accumulation in the wound region. This study successfully developed an OHA-DA/PAM/BC multifunctional hydrogel characterized by exceptional stretchability, self-healing, adhesiveness, injectability and antibacterial activity, demonstrating a significant impact on wound healing in vivo. These findings indicated that the OHA-DA/PAM/BC hydrogel holds substantial potential as wound dressings for future clinical applications.
研究领域:
生物材料学
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医学工程
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组织工程
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Sun Hengliang,Chen Yedan,Sang Xiaoqin,Liu Qingxiang,Yu Haoran,Hu Shaojun,Mao Yingji,Zhang Li
The immunoregulation of tissue-engineered bone has emerged as a prominent area for bone defect repair. While this field demonstrates considerable potential, effectively managing relevant factors and maintaining a balanced immune microenvironment in practical applications remain substantial challenges that require resolution. In this study, we tested a novel comprehensive hierarchical delivery system based on the requirements of a natural immune microenvironment for inflammatory factors, to optimize local immune responses through precise regulation of drug release. Quercetin (Que)-loaded zeolite imidazolate framework-8 (ZIF-8) nanoparticles were embedded in gelatin methacrylate (GelMA) to create a drug-release system featuring a Zn2+ shell and quercetin core. In vivo and in vitro studies demonstrated that this dual sustained-release hydrogel-ZIF-8 system can produce low concentrations of Zn2+ at an early stage, resulting in a mild anti-inflammatory effect and proliferation of bone marrow mesenchymal stem cells (BMSCs). Moreover, as inflammation advances, the release of quercetin works synergistically with Zn2+ to enhance anti-inflammatory responses, reconfigure the local microenvironment, and mitigate the inflammatory response that adversely impacts bone health by inhibiting the NF-κB signaling pathway, thereby promoting osteogenic differentiation. This system is pioneering for sequential microenvironment regulation based on its diverse anti-inflammatory properties, offering a novel and comprehensive strategy for bone immune regulation in the clinical treatment of bone defects.
研究领域:
生物医学工程
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免疫学
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骨组织工程
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Zhang Ting,Yu Yameng,Yuan Wei,Ren Zeqi,Cheng Yan,Wu Shuilin,Zheng Yufeng,Xia Dandan
Biodegradable Zn alloy have recently gained considerable attention for use in bone implants considering their biodegradability, attractive mechanical properties, and bioactivity. However, excessive corrosion of Zn alloy at the early stage of implantation may cause severe cytotoxicity, resulting in insufficient osseointegration, which hinders the clinical use of the Zn alloy. In this study, we designed a photothermal-controlled degradative hybrid coating as corrosion-protective barrier with the intention of preventing Zn ion burst release during the early stages of implantation and regaining the alloy's corrosion advantage later on. The coating consisting of Zeolite imidazole skeleton-encapsulated indocyanine green core-shell structured nanoparticles and polylactic coglycolic acid (ICG@ZIF-8/PLGA) on pristine Zn-0.8 (wt.%) Li (ZL) alloy. The electrochemical test results indicated that coating ZL with ICG@ZIF-8/PLGA might effectively reduce its corrosion current density (icorr) from 2.48 × 1 0 −5 A•cm−2 to 2.10 × 1 0 −8 A•cm−2. After near-infrared light irradiation, ICG@ZIF-8 heated PLGA coating to reach Tg, so that the coating was degrading, and the icorr of coated ZL alloy decreased to 2.50 × 1 0 −7 A•cm−2, thus restoring corrosion advantage. Both in vitro and in vivo investigations showed that coated ZL alloy had acceptable biocompatibility. Overall, the development of the photothermally controlled coating improved the Zn alloy's resistance to corrosion and allowed for the adjustment of the coated ZL alloy's rate of degradation through the use of 808 nm NIR laser irradiation.
研究领域:
生物医学工程
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材料科学
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生物材料
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组织工程
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2018
·Vol. 5
·期 3
·141-149
·
IF 0
Shen Taipeng,Zhu Wencheng,Yang Li,Liu Li,Jin Rongrong,Duan Jimei,Anderson James M,Ai Hua
Dendritic cell (DC)-based vaccines have shown promising therapeutic results in cancer and some immune disorders. It is critical to track in vivo migration behaviours of DCs and monitor the whole process dynamically and non-invasively. Superparamagnetic iron oxide (SPIO) nanoparticles are chosen for DC labelling under magnetic resonance imaging (MRI) because of their proven biosafety as contrast agents. However, when used for cell labelling, sensitive biological indicators such as cell autophagy may be helpful to better understand the process and improve the probe design. Here, lactosylated N-Alkyl polyethylenimine coated SPIO nanoparticles are used for DC labelling. This probe shows satisfactory cell labelling efficiency and low cytotoxicity. In this study, autophagy was used as a key factor to understand how DCs react to nanoparticles after labelling. Our results demonstrate that the nanoparticles can induce protective autophagy in DCs, as inhibition of the autophagy flux could lead to cell death. Meanwhile, the nanoparticles induced autophagy could promote DC maturation which is an essential process for its migration and antigen presentation. Autophagy induced DC maturation is known to enhance the vaccine functions of DCs, therefore, our results suggest that beyond the MRI tracking ability, this probe might enhance therapeutic immune activation as well.
研究领域:
纳米医学
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免疫学
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癌症研究
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分子生物学
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DOI
PubMed
Regenerative Biomaterials
·
2021
·Vol. 8
·期 2
·
IF 6.353
Zhang Jianhua,Hu Junfei,Chen Baoshu,Zhao Tianbao,Gu Zhipeng
Wound healing dressing is increasingly needed in clinical owing to the large quantity of skin damage annually. Excessive reactive oxygen species (ROS) produced through internal or external environmental influences can lead to lipid peroxidation, protein denaturation, and even DNA damage, and ultimately have harmful effects on cells. Aiming to sufficiently contact with the wound microenvironment and scavenge ROS, superabsorbent poly (acrylic acid) and antioxidant poly (ester amide) (PAA/PEA) hybrid hydrogel has been developed to enhance wound healing. The physical and chemical properties of hybrid hydrogels were studied by Fourier-transform infrared (FTIR) absorption spectrum, compression, swelling, degradation, etc. Besides, the antioxidant properties of hybrid hydrogels can be investigated through the free radical scavenging experiment, and corresponding antioxidant indicators have been tested at the cellular level. Hybrid hydrogel scaffolds supported the proliferation of human umbilical vein endothelial cells and fibroblasts, as well as accelerated angiogenesis and skin regeneration in wounds. The healing properties of wounds in vivo were further assessed on mouse skin wounds. Results showed that PAA/PEA hybrid hydrogel scaffolds significantly accelerated the wound healing process through enhancing granulation formation and re-epithelialization. In summary, these superabsorbent and antioxidative hybrid hydrogels could be served as an excellent wound dressing for full-thickness wound healing.
研究领域:
生物材料学
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医学
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组织工程学
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DOI
高影响因子
Regenerative Biomaterials
·
2026
·
IF 8.7
Li Ying,Liu Enze,Wang Pei,Liu Jiayi,Jin Weijie,Gao Zhen,Wu Jiayingzi,Wang Xiansong
The escalating global prevalence of obesity and its associated metabolic disorders now represent one of the most urgent public health crises. Despite the pressing need for safe and effective anti-obesity interventions, current therapeutic options are often limited by suboptimal efficacy and various side effects. Herein, we present a microneedle (MN)-based drug delivery platform, termed RES@hmPDA-Dpep/MN, for photothermal browning therapy (PBT) to combat obesity. This system integrates hemispherical mesoporous polydopamine (hmPDA) nanoparticles with resveratrol (RES) and an adipose-homing peptide (Dpep) for localized PBT. Under identical conditions, hmPDA maintains photothermal performance comparable to spherical mPDA, while exhibiting advantageous drug-loading behavior particularly under higher drug-feeding conditions. In addition, the platform supports localized delivery with NIR-triggered, on-demand RES release and thermogenic activation. In vivo studies demonstrate that the RES@hmPDA-Dpep/MN system effectively suppresses WAT expansion, reduces body weight by 16.1% ± 2.5%, and significantly improves obesity-associated metabolic dysfunctions, including hyperlipidemia and hepatic steatosis. Mechanistic analyses reveal marked upregulation of UCP1 and other key browning genes. Furthermore, the nanoplatform shows favorable in vitro biocompatibility and hemocompatibility, supporting its potential for further translational evaluation. Collectively, this PBT strategy may serve as a potential localized intervention for fat reduction and systemic metabolic improvement in the management of obesity and related metabolic disorders.
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DOI
高影响因子
Regenerative Biomaterials
·
2026
·
IF 8.7
Zhu Yanglong,Wei Yatian,Lan Zixun,Zhu Yuanzheng,Wu Hengyu,Zhong Tingting,Shen Xiang,Yang Ganghua,Yi Yangyan,Wang Xiaolei
Soft tissue defects represent a major challenge in plastic surgery, for which autologous fat grafting remains the standard treatment. However, in the early post-transplant period, the grafted fat tissue undergoes ischemia and hypoxia, leading to low and markedly unpredictable survival rates that consequently compromise the aesthetic outcomes. Nitric oxide (NO), an endogenous gasotransmitter, play a vital role in mediating angiogenesis and vascular remodeling. Therefore, we developed a near-infrared (NIR)-responsive hydrogel that releases NO on demand to promote adipocytes vascularization and thereby improve graft retention and enable precise volumetric filling. To ensure high-filling, the platform incorporates a photosensitive Gelatin Methacryloyl (GelMA) hydrogel inspired by the rapid solidification of “Frost Formation”. The hydrogel can be precisely delivered to the target area and photocrosslinked in situ to form a three-dimensional network that supports cell growth. Notably, this photocrosslinking process does not require ultraviolet (UV) irradiation, thereby eliminating the associated risks and significantly greatly enhancing the safety of adipose remodeling and filling technologies. Experimental studies conducted both in vitro and in vivo have validated that this hydrogel platform ensures precise grafting, promotes graft integration, and significantly improves the long-term survival of transplanted adipose tissue.
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2026
·
IF 8.7
Su Xiaomeng,Wang Han,Chen Hongbo,Ke Linnan,Han Qianqian
Cardiovascular diseases have become one of the major threats to human health. There is an urgent clinical need for small-diameter vascular grafts to achieve long-term implantation in the body. In previous studies, some progress has been made, but the prognosis is still poor. Heparin (HEP) is an anticoagulant that is widely applied in the clinical field and commonly used for anticoagulation modification of biomaterials. However, there is an important problem: HEP has almost no direct inhibitory effect on platelet adhesion, which may become one of the potential dangers after graft implantation. In recent years, it has been found that calcification is also a key factor affecting the long-term patency of small-diameter vascular grafts, which is often ignored. Therefore, in this study, based on HEP, procyanidin (PC) was introduced to modify the thermoplastic polyurethane (TPU) grafts fabricated by electrospinning to enhance the anticoagulation and anticalcification properties simultaneously. To verify the effects of drug modification on the material, the physical and chemical characterization, biocompatibility experiments and effectiveness tests of this vascular graft were conducted. The results indicated that the HEP-PC grafts could not only inhibit the excessive proliferation of human umbilical artery smooth muscle cells to some extent but also have excellent hemocompatibility. Particularly, in vivo experiments showed that this graft also achieved dual anticoagulation and delayed the calcification after implantation. These results suggested that HEP-PC-modified TPU might be a promising candidate for inhibiting intimal hyperplasia and anticalcification of small-diameter grafts.
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2026
·
IF 8.7
Shi Yaqiang,Liu Yaqin,Bai Xiaoyu,Gao Wentao,Ma Yawei,Wang Jieneng,Hu Yuan,Jiang Yongjie,Li Wei,Zhou Shifeng,Qi Quan
Myocardial Infarction (MI) is a leading cause of high global cardiovascular mortality and disability. Platelet-rich plasma (PRP), as a widely available autologous biological product rich in various growth factors, holds significant potential for promoting tissue healing and regeneration. However, its clinical application is limited by short in vivo retention time and transient therapeutic effects, necessitating the development of novel drug delivery systems to enhance its clinical value. In this study, a novel injectable PRP-gel composite was developed. Based on the Schiff base reaction, the composite gel exhibits excellent biocompatibility, biodegradability, appropriate mechanical properties, and enables controlled release of multiple growth factors from PRP. Results at 28 days post-intramyocardial injection demonstrated that, compared with the control group, the PRP-gel composite effectively promoted angiogenesis, inhibited cardiac cell apoptosis and injury, significantly reduced infarct size, decreased myocardial fibrosis, and preserved ventricular wall thickness, ultimately leading to a marked improvement in cardiac function. In summary, the composite gel developed in this study provides an optimized strategy for the clinical application of PRP and represents a promising therapeutic approach for myocardial regeneration following MI.
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DOI
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Kang Shaoyang,Ding Sheng,Lyu Donghao,Gao Rui,Peng Sirui,Liu Jing,Zhang Chuangnian,Feng Zujian,Huang Pingsheng,Kong Deling,Wang Weiwei
Artificial energy supply modules that can produce adenosine triphosphate (ATP) through natural or synthetic structures, are crucial for supporting artificial cells with therapeutic purposes. However, their advanced biomedical application is hindered by poor stability, short lifespan and low output efficiency. In this study, an artificial light-controlled energetic module with long-term activity, termed thylakoid-loaded microgel (TM), is created by encapsulating spinach-derived thylakoid into alginate/gelatin microgels. The TM effectively retains the photosynthetic light reactions of thylakoids, including the electron transfer capacity of photosystem II and ATP production, and releases the produced ATP to external environment. As a proof of concept, the TM successfully drives the luciferin/luciferase reaction both within and outside the microgel compartment. In addition, the encapsulated thylakoids exhibit a significantly prolonged activity, with the high photosystem II activity and ATP production lasting for at least 96 hours. The long-term activity is attributed to the oxidation shielding efficacy, protein and pigment degradation inhibition, and membrane structural stabilization. This study presents a strategy for developing artificial energy supply modules with efficient energy output and long-term activity, holding great promise in artificial cell construction and biosynthesis.
研究领域:
合成生物学
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生物能源
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生物医学工程
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光合作用研究
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生物材料
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Yan Xin,Li Xiangdong,Zhang Qi,He Xinlin,Chen Qi,Mai Sui
Human dental pulp stem cells (hDPSCs) exhibit replicative senescence during in vitro expansion, leading to a reduction in osteogenic differentiation capacity and thereby limiting their potential for bone defect regeneration. Magnesium ion (Mg2+), one of the most abundant divalent cations in the human body, is involved in numerous physiological processes. Mg2+ deficiency has been closely associated with bone fragility and various systemic aging-related diseases, underscoring its critical role in aging and bone metabolism. However, the effects of Mg2+ on mesenchymal stem cell (MSCs) replicative senescence remain poorly understood. In this study, we developed magnesium-doped bioactive glass (Mg-BG) powder with a graded magnesium doping ratio through the sol-gel method, and characterized the pore structure and ion release profiles of each Mg-BG group. We demonstrated that 20 Mg-BG (Mg-BG containing 20 mol% MgO) can effectively reverse the replicative senescence of hDPSCs, improve mitochondrial function, reduce ROS levels, and enhance the expression of surface markers associated with differentiation, migration, and adhesion in replicatively senescent hDPSCs, thereby enhancing their osteogenic differentiation potential. Furthermore, in vivo experiments using a rat calvarial defect model also confirmed that 20 Mg-BG significantly enhances bone defect repair mediated by replicatively senescent hDPSCs. Mechanistically, we found that the IKBKGP1-mediated NF-κB pathway may play a key role in this process, as revealed by transcriptome sequencing. These findings indicate that Mg-BG could serve as an effective, innovative approach to reverse replicative senescence in hDPSCs and enhance their bone defect repair capabilities.
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PubMed
高影响因子
Regenerative Biomaterials
·
2016
·Vol. 3
·期 4
·217-222
·
IF 0
Rong Lei,Zhang Chi,Lei Qi,Hu Ming-Ming,Feng Jun,Shu Hong-Bing,Liu Yi,Zhang Xian-Zheng
Hydrogen peroxide (H 2 O 2 ) detection in biological systems is of significant importance, which act as critical second messenger in fundamental biological processes. Here, we report on a chemoselective fluorescent naphthylimide peroxide probe (NPP) for the H 2 O 2 detection in vitro and in vivo. NPP is a phenylboronic acid-caged chromophore that selectively responds to H 2 O 2 through a self-immolate mechanism. NPP exhibited high sensitivity and selectivity to H 2 O 2 with distinctive fluorescence change due to the excellent two-photon excitation property, which permits the facile detection of inflammation produced H 2 O 2 and offers chance to monitor the inflammatory stages in diseased cells.
研究领域:
生物化学
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分子生物学
·
分析化学
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DOI
PubMed
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