RENAL FAILURE
·
2022
·
IF 3.222
Qi Xiao
Background To prove the internal connection, we deciphered the effect of cinnamaldehyde on kidney senescence through establishing animal and cell models.Methods In vivo, a rat senescence model was constructed using D-galactose (D-gal), and the modeled rats were further treated with cinnamaldehyde. In vitro, rat renal tubular epithelial cells (NRK-52E) were transfected with miR-155 mimic or inhibitor and then treated with cinnamaldehyde, D-gal or PI3K inhibitor (LY294002). The serum levels of blood urea nitrogen (BUN) and serum creatinine (Scr) of the rats were measured by an automatic biochemical analyzer. Pathological changes of kidney were determined by hematoxylin-eosin staining. The senescence and viability of NRK-52E cells were assessed by SA-β-gal staining and CCK-8 assay, respectively. The levels of miR-155, p-PI3K/PI3K, p-Akt/Akt, LC3B (LC3-II and LC3-I) and Beclin1 were detected by qRT-PCR, immunohistochemistry, or western blot.Results D-gal elevated the levels of BUN, Scr and miR-155 in the kidney, induced the renal pathological damage, inhibited the cell viability, increased the numbers of SA-β-gal-, LC3B- and Beclin1-positive cells and upregulated the levels of LC3-II/LC3-I and Beclin1 both in the kidney and cells. Cinnamaldehyde reversed D-gal-induced effects on the kidney and cells, and moreover, the cinnamaldehyde-induced anti-D-gal effects on cells could be suppressed by miR-155 mimic but promoted by miR-155 inhibitor. LY294002 potentiated D-gal-induced effects, and reversed cinnamaldehyde- and miR-155 inhibitor-caused impacts on the PI3K/Akt pathway and LC3-II/LC3-I level in D-gal-induced cells.Conclusion Cinnamaldehyde attenuates kidney senescence and injury through PI3K/Akt pathway-mediated autophagy via downregulating miR-155.
研究领域:
药理学
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分子生物学
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肾病学
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细胞生物学
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DOI
PubMed
高影响因子
Frontiers in Cardiovascular Medicine
·
2022
·
IF 5.846
Wenqing Gao,Han Wang,Yanwu Liu,Qin Tang,Peng Wu,Tingting Lin,Tong Li,Di Sun
Thromboembolism caused by the use of extracorporeal membrane oxygenation (ECMO) remains common among patients with existing heart diseases and contributes to significant morbidity and mortality during the COVID-19 pandemic. Various surface modification strategies have been proposed, showing that the methacrylated alginate (MA-SA) hydrogel layer is transparent, which aids the observation of the thromboembolism from the inner wall of the tubing. In the combined dynamic and static blood of ECMO tubing inner surface in vitro experiments, it was also demonstrated that the adhesion of blood clots to the surface of vessels was remarkably reduced, and the MA-SA-based hydrogel coating could significantly prolong the activated partial thrombin time and block the endogenous coagulation. The favorable properties of natural polysaccharides of hydrogel coatings make them the best surface material choices to be applied for blood-contacting medical devices and significantly improve anticoagulant performance.
研究领域:
生物材料
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生物医学工程
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止血与血栓
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医疗器械
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Hui Guo,Jiangtao Ma,Yanli Zhang,Yan Mao,Ziwei Hu,Ying Lin,Feng Yu,Wei Wang,Yaling Liu
An autoimmune condition known as systemic lupus erythematosus (SLE) is characterized by B cell hyperresponsiveness and persistent generation of pathogenic autoantibodies that cause damage to various organs and tissues. The treatments available today are either ineffective or have adverse effects. The dysregulation of B cell activation is crucial for the emergence of SLE. MiR-7 explicitly targeted PTEN mRNA in B cells. Treatment with antagomiR-7 reduced B cell hyperresponsiveness and prevented the onset of lupus. As a result, inhibiting miR-7 may be used therapeutically to treat SLE. We developed a SA (sialic acid)-poly (D, L-lactide-co-glycolide) (SA-PLGA) nano delivery system to deliver antagomiR-7 into splenic B cells since the stability and targeted delivery of miRNA remain significant challenges in vivo . Results show that SA-PLGA nanoparticles (SA-PLGA@antagomiR-7) loaded with antagomiR-7 display good biocompatibility and shield antagomiR-7 from degradation, extending the miRNA’s duration in circulation in vivo . Additionally, in MRL/Ipr lupus mice, SA-PLGA@antagomiR-7 is successfully delivered to the splenic B cells and preferentially enriched in the diseased spleen in MRL/Ipr lupus mice. The SA-PLGA@antagomiR-7 NPs therapy effectively decreases immunological abnormalities, normalizes splenic B cell subtypes, and suppresses B cell activation. The antagomiR-7 NPs exhibit excellent therapeutic efficiency and high biosafety collectively, which may result in a more effective treatment for SLE.
研究领域:
免疫学
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纳米技术
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药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jinghai Zhang,Kangjun Ji,Zirui Wang,Wei Shi,Zhongkai Zhao,Xiao Du,Xiaogang Hao
The rapid growth in lithium demand for battery applications has driven the need for safe and efficient extraction technologies from salt lake brines. Current methods face challenges including low recovery rates, slow adsorption kinetics, and environmental concerns from organic solvent usage. To address these limitations, the environmentally friendly electrochemically switched ion exchange (ESIX) system using a three-dimensional LiMn 2 O 4 /C/SA-c-PEO@GF electrode was developed. The electrode employs sodium alginate (SA) and polyethylene oxide (PEO) as chemically cross-linked binders on a graphite felt (GF) current collector. In the constant current-constant voltage coupling drive mode, the system achieved 97% lithium extraction efficiency with an adsorption capacity of 3.89 mg·g −1 and a Li + /Mg 2+ separation coefficient of 191, the energy consumption was 14.51 Wh·mol −1 and 485 mg·L −1 Li + solution was obtained from actual Zarkhan Salt Lake brine (Mg 2+ /Li + : ∼426.6) by one-step concentration. This study shows the LiMn 2 O 4 /C/SA-c-PEO@GF electrode with high extraction efficiency and fast adsorption rate, providing a simple and efficient method for lithium recovery from salt lake brine with high Mg 2+ /Li + ratio.
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高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 9
·1164-1171
·
IF 2.896
Minglei Lu,Qiyi He,Yingying Zhong,Junkang Pan,Zhiting Lao,Mingxia Lin,Tiantian Wang,Xiping Cui,Jinlong Ding,Suqing Zhao
Progesterone (P4) belongs to a factor that affects stress response and is a potential carcinogen, and saliva levels are expected to be a standard measurement for clinical diagnosis. In this study, a new type of nanoflower with both recognition functionality and catalytic substrate ability was prepared by copper phosphate, Pt/IrO2 nanocomposites (Pt/IrO2 NPs), streptavidin (SA) and horseradish peroxidase (HRP) via a one-pot co-precipitation strategy. Due to the enhanced catalytic activity and stability of Pt/IrO2@SA@HRP nanoflowers, we developed a powerful and sensitive multiple-catalysis ELISA to monitor progesterone in saliva. Multiple-catalysis ELISA based on a specific antibody and Pt/IrO2@SA@HRP nanoflowers exhibited a linear interval range from 0.217 ng mL−1 to 7.934 ng mL−1. The median inhibitory concentration (IC50) for progesterone is 1.311 ng mL−1 and the limit of detection (LOD = IC10) is 0.076 ng mL−1 in the proposed method. Satisfactory recoveries were in a range of 79.6–107% with an acceptable coefficient of variation (below 10.6%). Results of the multiple-catalysis ELISA and LC-MS/MS had a good coincidence. Our result unraveled that multiple-catalysis ELISA is a potentially serviceable tool for the detection of progesterone in saliva.
研究领域:
分析化学
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生物化学
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纳米技术
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临床诊断
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产品关联
DOI
PubMed
高影响因子
EXPERIMENTAL CELL RESEARCH
·
2025
·
IF 3.5
Zhen Yang,Feng Yan,Jiangwei Yuan,Manjun Yang,Jinyu Wang,Changqiao You,Kaiqun Ren
Background Cellular senescence refers to a condition where cells permanently cease division while maintaining metabolic activity. Doxorubicin (Dox) is known as an agent of induction of cellular senescence. This study aimed to explore the potential role of human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-Exo) in mitigating Dox induced senescent. Method NIH3T3 cells were treated by various concentrations of Dox with or without hucMSC-Exo, cell morphology, viability, migration, senescence-associated SA-β-Gal staining were monitored. Cellular senescence was induced in C57BL/6J mice via administration of 5 mg/kg Doxorubicin, followed by treatment with hucMSC-Exo or metformin. Assessments included body weight, liver and kidney weight, colon length, SA-β-Gal staining of kidney and skin, molecular biomarkers of aging such as p16 INK4A , p53, and p21 Waf1/Clip1 to evaluate senescence status. Result We found that after the treatment of exosomes or metformin improved several aging-related phenotypes in both mouse and cellular models, including increases in body weight, liver and kidney weights, and the reduction of SA-β-Gal positive cells in kidney and skin tissues as well as cell models. At the molecular level, hucMSC-Exo resulted in the downregulation of inflammatory factors and senescence markers in liver and kidney tissues as well as cell models. Conclusion Our study demonstrates hucMSC-Exo may ameliorate Dox induced senescence either in NIH3T3 cells or in mice.
研究领域:
干细胞研究
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药理学
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细胞生物学
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老年医学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Han Cui,Xuejiao Wu,Yuchen Wang,Yafei Zhang,Juan Luo,Dandan Zhou
This study investigates the migration of lead (Pb) during soil electrokinetic remediation (EKR) with sodium alginate (SA), providing important data on ion orientation and Pb removal efficiency. A 1 mV/cm voltage was applied to kaolinite contaminated with Pb²⁺, and SA was applied to regulate Pb migration. Pb was detected in both the anolyte and catholyte, with varying molar Pb/C ratios. In the anode and cathode chambers, the molar ratios reached 1:7.79 and 1:2.64, respectively. The addition of Pb 2+ disrupted the stable structure of SA, and density functional theory (DFT) calculations combined with X-ray Absorption Fine Structure (XAFS) analysis showed that Pb²⁺ forms dual-ridentate (positively charged) and quad-/hexaridentate (negatively charged) complexes with SA, thereby influencing Pb migration toward the corresponding electrodes. Additionally, Pb in the soil transformed into immobile particulate states, such as PbO 2 , Pb 3 O 4 , and PbO. The formation of these particulate phases and conversion of mobile Pb phases to the anode reduced the Pb migration ability at the cathode. These findings offer fresh perspectives on EKR’s application for heavy metal soil remediation, particularly six-period metals on the periodic table, improving our understanding of Pb behavior during electrokinetic treatments.
研究领域:
环境科学
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土壤化学
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电化学
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重金属修复
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材料科学
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地球化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Hongmei Xiang,Jiao Meng,Wubin Shao,Dan Zeng,Jin Ji,Peiyi Wang,Xiang Zhou,Puying Qi,Liwei Liu,Song Yang
The excessive use of non-renewable formulations, low utilization efficiency, and eco-unfriendly behavior are serious disadvantages of traditional pesticide formulations. Herein, a scalable and sustainable biopolymer-based core–shell (CS) nanocarrier loaded with an anti-viral candidate agent, Bingqingxiao (BQX), was investigated for plant disease management. Preliminary [email protected] @SWP nanoparticles ( [email protected] @S NPs) were manufactured through the noncovalent self-assembly of pyridine-grafted poly (hydroxyethyl methacrylate) (PyPHEMA) and natural soy whey protein (SWP), and exhibited a stable spherical morphology with a diameter of approximately 130 nm. Notably, encapsulation of BQX into the NPs increased the ultra-violet (UV) resistance of the compound by 8.7-fold, and the nanocarrier [email protected] ( [email protected] ) enhanced the BQX foliar surface wettability, resulting in a slow, urea-responsive release. In vivo studies revealed that the protective activity of [email protected] @S NPs was 1.4-fold higher than that of BQX against tobacco mosaic virus (TMV), and the antiviral efficiency was further improved by stimulation with 0.2 M urea. Furthermore, foliar application of [email protected] @S NPs activated host plant defense responses by upregulating the expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes. Interestingly, the developed [email protected] @S NPs act as plant nutrition to enhance the crop fresh and dry weight by 24.7% and 19.9%, respectively. Consequently, [email protected] is a promising biocompatible carrier that can enhance the potency of various pesticides, synergistically improve plant disease resistance, and simultaneously act as a fertilizer for crop growth.
研究领域:
农业科学
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纳米技术
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植物病理学
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生物技术
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产品关联
DOI
高影响因子
Regenerative Biomaterials
·
2023
·
IF 6.7
Zheng Zexiang,Yang Xing,Fang Min,Tian Jinhuan,Zhang Shuyun,Lu Lu,Zhou Changren,Xu Changpeng,Qi Yong,Li Lihua
Chronic diabetic wound healing remains a formidable challenge due to susceptibility to bacterial infection, excessive oxidative stress, and poor angiogenesis. To address these issues, a sodium alginate (SA) based photothermal hydrogel dressing with multifunction was fabricated to facilitate wound treatment. Ceria nanoparticles (CeO2NPs) was synthesized, and their antibacterial performance by near-infrared light triggered photothermal effects was first studied and verified in this work. In addition, to release CeO2NPs to achieve antioxidation and pro-vascularization, thermosensitive gelatin (Gel) was utilized to embed the nanoparticles in advance and then composited in SA hydrogel networks. SA network was finally strengthened by acid soaking to form partially crystalline regions to act as natural crosslinkers. Results showed that the Gel/SA/CeO2 hydrogel displayed temperature-responsive release of CeO2NPs, significant antibacterial and antioxidative activity, as well as the ability to remove without injury and promote infected diabetic wound healing with low cytotoxicity, according to antibacterial investigations, cell studies, and in vivo animal studies. This research offers not only a successful method for quickening the healing of diabetic wounds but also a fresh approach to the general use of CeO2NPs.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
·
伤口愈合
·
生物材料
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Dahe Qiao,Junyan Zhu,Xiaozeng Mi,Hui Xie,Mingtao Shu,Minyi Chen,Rui Li,Shengrui Liu,Chaoling Wei
Withering time of fresh leaves is a key factor affecting the stability of flavor quality of Taiping Houkui tea (TPHK). It's effect and mechanism on the formation of flavor in TPHK remain unelucidated. Here, metabolomics and transcriptomics were combined to comprehensively investigate changes in the main flavor compounds and the expression patterns of related genes under different withering times. The results showed that withering had the greatest influence on the content of most amino acids and aroma volatiles. The relative content of most volatiles was observed to be highest at 6–8 h of withering time, which was consistent with the results from the sensory evaluation of aroma quality. Plant hormone signal transduction pathways played a key role in the variation of the flavor compound content under different withering times, especially ABA and JA signals. WGCNA demonstrated that ABA, JA, IAA, SA and different flavor substances were significantly associated with specific modules. JA may be involved in the metabolism of theanine during withering, while ABA may be involved in the accumulation of aromatic amino acids and main aroma components of TPHK. This research may serve as a reference for the optimization of the processing technology for TPHK.
研究领域:
植物学
·
遗传学
·
食品科学
·
分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·Vol. 141
·期 35
·e55889
·
IF 2.7
Xiaohong Wang,Bin Yang,Yue Zhang,Yuqing Yang,Huijie Lu,Hao Wang,Ziyi Wang,Jiasheng Qian,Ru Xia,Ning Jia,Yuchao Ke
Phase-change materials (PCMs) have become a hot spot in the development of modern thermal management materials because of their absorbing and releasing heat while keeping the temperature constant during phase change process. However, their relatively low thermal conductivity (TC) considerably restricts their further application. In this work, graphene nanosheets (GNS) were initially nickel-plated to increase the contact area between adjacent GNS (in the form of Ni@GNS) by constructing a “point-surface” structure. Then, the prepared Ni@GNS was coated with polydopamine (PDA) to prepare PDA-Ni@GNS, which helped to effectively reduce the interface thermal resistance (ITR) between the filler and the matrix, and also made the prepared filler more easily dispersed in the polymer matrix. By constructing well-defined three-dimensional (3D) thermally conductive pathways in the matrix, the PCMs exhibited excellent TC and effectively prevented the leakage of polyethylene glycol (PEG) even at low filler loading. The results of thermogravimetry (TG), differential scanning calorimeter (DSC), and cyclic DSC tests jointly showed that the PDA-Ni@GNS/CNF-C/SA/PEG PCMs displayed excellent thermal storage properties, high latent heat as well as good cyclic thermal stability. The present study provides a facile way of preparing PEG-based PCMs, which are applicable in thermal management area.
研究领域:
材料科学
·
热能工程
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Hanxin Qian,Zhan Wu,Zhouyu Huang,Menglu Lu,Jiayuan Xiang,Fangfang Tu,Zheyu Jin,Jianping Xu,Yongping Gan,Xinping He,Hui Huang,Xinhui Xia,Yang Xia,Wenkui Zhang,Jun Zhang
Sodium-ion batteries (SIBs) are promising for large-scale energy storage due to their high cost-effectiveness and safety. Alloy anodes exhibit significantly higher specific capacities compared to those of carbonaceous anode materials, holding great promise for enhancing the energy density of SIBs. However, their practical application is severely hindered by substantial volume expansion, sluggish reaction kinetics, and continuous fracture reformation of the solid-electrolyte interphase (SEI). Here, a multifunctional composite binder system was designed for the Sn anode, which was composed of poly(ethylene oxide) (PEO), sodium alginate (SA), and sodium bis(trifluoromethylsulfonyl)imide (NaTFSI). The PEO-SA-NaTFSI (PSN) binder integrates ternary synergistic functions: robust adhesion between the active material and current collector, enhanced Na+ transport kinetics, and formation of a stable NaF-rich SEI. Therefore, the Sn-PSN anode achieves an outstanding sodium storage performance with a high capacity of 583.4 mA h g–1 at a current density of 10 A g–1 and 77% capacity retention after 1500 cycles. In addition, the Sn-PSN||Na3V2(PO4)3 full battery can maintain 81% capacity after 200 cycles with an electrode energy density of 169.0 W h kg–1. This work provides a multifunctional binder design strategy with broad application prospects for high-performance anodes of SIBs.
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DOI
PubMed
高影响因子
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