FOOD CONTROL
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2024
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IF 5.6
Yifei He,Jinxiang Wei,Lili Zhang,Yu Xia,Zhouping Wang,Junsong Yang
In this study, a dual-mode SERS-fluorescent aptasensor based on a DNA walker was developed for Shigella flexneri ( S . flexneri ). Fe@SiO2@QDs were connected with H1 and the walking chain (hybrid strands of aptamer and cDNA) to fabricate fluorescent probes, and Ag R6G @SiO 2 were connected with H2 to fabricate SERS tags. Aptamer (Kd = 42.6 ± 3.16 nM) selective binding of S . flexneri , left the walking chain. The Zn 2+ -specific DNAzyme in the cDNA was exposed thus driving the DNA walker. The sequence on H1 containing BHQ3 was cleaved from fluorescent probes to restore the fluorescence. The remaining H1 sequence could bind to the H2 to generate Raman signals. The detection limit of the fluorescence signal was 11 CFU/mL, and the Raman signal was 3 CFU/mL. In addition, the aptasensor was successfully applied to the detection of actual samples with recovery ranges of 98.35–107.27% and 97.57–105.91% for the fluorescence and SERS methods, respectively.
研究领域:
生物化学
·
分子生物学
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DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2013
·Vol. 19
·期 28
·9211-9217
·
IF 5.831
Chuhong Zhu,Guowen Meng,Qing Huang,Yao Zhang,Haibin Tang,Yiwu Qian,Bin Chen,Xiujuan Wang
Graphical SERS up : Parallel face-exposed Ag nanoplates vertically grown on micro-hemisphere surfaces have been prepared by firstly electrodepositing the micro-hemispheres assembled by Ag nanoplates, the planar surfaces of which are stuck together, on indium tin oxide (ITO) substrates, and then Ostwald ripening the as-electrodeposited micro-hemispheres in water (see figure). Ag nanoplates, as two-dimensional plasmonic nanostructures, have attracted intensive attention due to their strong shape-dependent optical properties and related applications. Here parallel face-exposed Ag nanoplates vertically grown on micro-hemisphere surfaces have been achieved by firstly electrodepositing the micro-hemispheres assembled by Ag nanoplates, whose planar surfaces are stuck together, on indium tin oxide substrates, and then Ostwald ripening the as-electrodeposited micro-hemispheres in water. The sizes of the nanoplates and the gaps between the neighboring nanoplates have been tailored by tuning the Ostwald-ripening duration, so that the SERS activity of the micro-hemispheres has been remarkably improved. The improved SERS activity can be well explained by our systematic finite-element simulation. Therefore, Ostwald ripening offers a route to the synthesis of Ag nanoplates, and the optimization of plasmon coupling and SERS activity of nanostructure-assembled systems.
研究领域:
材料科学
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表面增强拉曼光谱
·
纳米技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Tianxing Fang,Qingyi Wei,Erwen Wu,Hongbin Pu
Malachite green (MG), with teratogenic and reproductive toxicity, is widely misused as an antimicrobial agent, posing a serious threat to animal and human health. Therefore, this study developed a novel recyclable Surface-enhanced Raman scattering (SERS) hydrogel substrate, TiO 2 /MXene/PAA-PAM@AuNPs (TMPA), by forming a dual hydrogen-bond network using a PAA-PAM hydrogel matrix with TiO 2 /MXene and embedding AuNPs, for detecting MG residues in aquatic products. This hydrogel exhibits high sensitivity, with a detection limit for MG of 8.13 × 10 −6 mg/L. Additionally, it can completely degrade surface-adsorbed MG within 60 min under light irradiation, achieving self-cleaning and enabling reuse for more than seven cycles, and can be stored for over 50 days while maintaining its SERS activity due to the support and preservation provided by the hydrogel network. Additionally, this substrate can rapidly detect MG residues on or within fish, offering a new strategy for monitoring MG residues in aquatic products.
研究领域:
食品安全
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分析化学
·
环境科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
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IF 4.3
Heng Guo,Hongji Li,Mengyang Xu,Juan Zhou,Dan Zhang,Dandan Wang,Wei Sun
In this study, a core–shell structured bimetallic nano-cube, Au@Ag NCs, was prepared by seed-mediated growth procedure. The array structure of Au@Ag NCs was achieved at the interface through the autonomous assembly technique at the three-phase boundary. Employing polydimethylsiloxane (PDMS) as a flexible carrier, the array structure was effortlessly transferred to the PDMS membrane, bypassing the need for rigid substrates through a simple “pasting” method. This yielded a highly flexible and transparent SERS substrate with an array structure (Au@Ag NCs/PDMS membrane, AAP). In order to promote the selective detection property to the practical samples, molecularly imprinted polymers (MIPs) were coated on the surface of membrane to prepare the imprinted membrane (Au@Ag NCs/PDMS-MIMs, AAP-MIMs). It was demonstrated from the results that the AAP-MIMs exhibited high SERS sensitivity, stability, and uniformity. Furthermore, the flexible substrate possessed commendable mechanical strength, and facilitated the detection of analytes on irregular surfaces. In summary, this substrate held promising potential for practical on-site detection and analysis of specific target substances.
研究领域:
分析化学
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材料科学
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环境科学
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DOI
PubMed
高影响因子
Journal of Nanomaterials
·
2015
·
IF 1.644
Song Yang,Tian Zeng,Yaling Li,Jun Liu,Qian Chen,Ji Zhou,Yong Ye,Bin Tang
The fast detection and removal of organic dyes from contaminated water has become an urgent environmental issue due to their high toxicity, chemical stability, and low biodegradability. In this paper, we have developed graphene oxide decorated Fe3O4@SiO2 (Fe3O4@SiO2-GO) as a novel adsorbent aiming at the rapid adsorption and trace analysis of organic dyes followed by surface enhanced Raman scattering (SERS). The structure and morphology of the nanocomposites were characterized by transmission electron microscopy (TEM), Fourier infrared spectrometry (FT-IR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). The obtained nanocomposites were used to adsorb methylene blue (MB) in aqueous solution based on π-π stacking interaction and electrostatic attraction between MB and GO, and the adsorption behaviors of MB were investigated. Moreover, the obtained nanocomposites with adsorbed dyes were separated from the solution and loaded with silver nanoparticles for SERS detection. These nanocomposites showed superior SERS sensitivity and the lowest detectable concentration was 1.0 × 10−7 M.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
Chemosensors
·
2022
·Vol. 10
·期 9
·373
·
IF 4.229
Junfang Hao,Min He,Bin Liu,Jianhui Yang
The preparation and self-assembling of monodisperse gold nanoparticles (Au NPs) is of great significance for its SERS application. According to the oleylamine-reduced method, oleylamine (OAm) serves as the reducing agent and stabilizing agent, and the effects of different reaction parameters such as solvent and temperature on the size and dispersity of Au NPs have been evaluated. The Au NPs synthesized with toluene as the solvent have the best dispersity and narrowest particle size distribution with adjustable sizes. The particle size gradually increases with the increase in reaction temperature. The highly ordered self-assembly film of Au NPs was employed as surface-enhanced Raman scattering (SERS) substrate for the probing molecule of rhodamine 6G. The Au substrate exhibits excellent spatial uniformity and SERS reproducibility, which indicates its practicability as a substrate. This study provides a simple synthesis strategy of highly ordered monodispersed Au NPs, which can serve as a SERS substrate with excellent spatial uniformity and SERS re-producibility.Keywords:gold nanoparticles;solvent effect;monodispersity;surface-enhanced Raman scattering (SERS)
研究领域:
纳米材料
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分析化学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Fengrui Xu,Fengguo Ma,Zhaozhao Ding,Liying Xiao,Xiaoyi Zhang,Qiang Lu,Guozhong Lu,David L. Kaplan
The formation of hot spots is an effective approach to improve the performance of surface-enhanced Raman scattering (SERS). Silk nanoribbons (SNRs), with a height of about 1–2 nm, and Au nanoparticles (AuNPs) were assembled by electrostatic interactions to introduce sandwich hot spot structures. These sandwich structures were optimized by tuning the ratio of SNRs and AuNPs, resulting in strong SERS signals with a sensitivity of 10–13 M and enhancement factor (EF) of 5.8 × 106. Improved SERS spectrum uniformity with relative standard deviation (RSD) about 11.2% was also achieved due to the homogeneous distribution of these hot spot structures. The inherent biocompatibility of SNRs and facile fabrication processes utilized endowed the SERS substrates significant benefits toward biomedical applications, confirmed by cytocompatibility and improved SERS bioimaging capacity in vitro. The results of this study suggest the feasibility of forming high performance bioimaging systems through the use of naturally derived materials with special nanostructures.
研究领域:
材料科学
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纳米技术
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生物医学工程
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分析化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoxiao Dong,Chang Lu,Shuaijie Guo,Siqi Zhang,Xuelian Hu,Wenliu Wang,Yiwei Tang,Shuo Wang,Xiuying Liu
A magnetic NiFe 2 O 4 @TiO 2 @Au composite was successfully synthesized via layer-by-layer assembly approach and exhibited excellent SERS performance. The composite was functionalized with an aptamer modified with Cy3, serving as the Raman signal reporter, and was hybridized with complementary DNA oligonucleotides (cDNA) to construct the NiFe 2 O 4 @TiO 2 @Au@ssDNA SERS aptasensor. This aptasensor enabled the development of a sensitive, specific, and straightforward SERS assay for acetamiprid detection, exhibiting good accuracy. Under optimized conditions, an inverse correlation was established between the acetamiprid concentration and the Raman intensity at the characteristic peak of 1132 cm −1 , spanning a range of 10 −1 -10 4 nmol/L with a LOD of 8.4 × 10 −1 pmol/L. Standard addition experiments to three types of leafy vegetables samples yielded recoveries ranging from 94.30 % to 111.83 %, with a relative standard deviation of <5.56 %. This method shows potential for detecting other hazardous substances in food and environmental samples by simply changing the corresponding aptamers and cDNA.
研究领域:
分析化学
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食品安全
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环境科学
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DOI
PubMed
高影响因子
Plasmonics
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2024
·
IF 3.3
Tian Xiaoliang,Yan Weizhou,Liu Bin,Yang Jianhui
The fabrication of sensitive and homogeneous surface-enhanced Raman scattering (SERS) substrates is the key to its practical application. In this paper, flower-like silver nanoparticles (Ag NPs) were successfully synthesized in water/ethanol/n-octanol emulsions by an assistant of citric acid and sodium citrate. The size and surface roughness of flower-like Ag NPs can be adjusted by changing the experimental parameters, such as the ratio of citric acid to sodium citrate, and the concentration of ascorbic acid. The resulting flower-like Ag NPs were constructed to form a homogeneous single-layer film by a three-phase interfacial self-assembly method. The performance of the film as SERS substrate was investigated using Rhodamine 6G as a probe molecule, and the enhancement factor was as high as 2.7 × 10 7 . In addition, the SERS substrate exhibits excellent repeatability, with a relative standard deviation of 6.5% for point-to-point Raman intensity changes. The current research will provide a new strategy for SERS sensors in food, environment, and medical applications.
研究领域:
材料科学
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分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Ying Chen,Hongmei Liu,Xiuting Li,Shiwei Tang,Chenjie Gu,Guodong Wei,Tao Jiang,Xingfei Zhou
Molybdenum disulfide (MoS 2 ) with promising photocatalytic activity has attracted great attention in constructing recyclable SERS substrate. Reduced graphene oxide (RGO) was chose here to in-situ grow MoS 2 nanoflowers and effectively adjust the active area of the substrate. After the decoration of Ag nanoparticles by photo-reduction, a sophisticated RGO@MoS 2 @Ag ternary nanocomposite with a maximizing SERS enhancement factor (EF) of 8.6 × 10 6 and a prominent solar photocatalytic efficiency was demonstrated. Such superior features of the nanocomposite were revealed to be endowed by the synergistic effect of the three components including the active sites of MoS 2 , the “Schottky barrier” and “hot spots” between MoS 2 and Ag, together with the molecular enrichment surface and chemical enhancement ability of RGO . An ultra-sensitive SERS-based detection and a reliable photocatalytic degradation of mixed trace molecules were finally achieved by the developed optimal ternary nanocomposite, which can meet manifold requirements for the routine supervision of biochemical molecules.
研究领域:
材料科学
·
分析化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Qiuwen Huang,Na Zhou,Jiayi Peng,Xuanjiang Zeng,Lijuan Du,Yan Zhao,Xiaojun Luo
Background The COVID-19 pandemic, caused by the novel coronavirus, has had a profound impact on global health and economies worldwide. This unprecedented crisis has affected individuals, communities, and nations in diverse manners. Developing simple and accurate diagnostic methods is an imperative task for frequent testing to mitigate the spread of the virus. Among these methods, SARS-CoV-2 antigen tests in clinical specimens have emerged as a promising diagnostic method for COVID-19 due to their sensitive and accurate detection of spike (S) protein, which plays a crucial role in viral infection initiation. Results In this work, a dual-signal amplification surface enhanced Raman scattering (SERS)-based S protein biosensor was constructed based on Au NPs/COFs and enzyme-free catalytic hairpin assembly (CHA) amplification method. The approach relies on a released free DNA sequence (T), which is generated from the competition reaction between Aptamer/T and Aptamer/S protein, to trigger a CHA reaction. Due to the high binding affinity and selectivity between the S protein and its aptamer, CHA process was triggered with the maximum SERS tags (H2-conjugated Au@4-mercaptobenzonitrile@Ag) anchored onto Au NPs/COFs substrate surface. This SERS platform could detect the S protein at concentrations with high sensitivity (limit of detection = 3.0 × 10 −16 g/mL), wide detection range (1 × 10 −16 to 1 × 10 −11 g/mL), acceptable reproducibility (relative standard deviation = 7.01 %) and excellent specificity. The biosensor was also employed to detect S protein in artificial human salivas. Significance Thus, this study not only developed a novel Au NPs/COFs substrate exhibiting strong SERS enhancement ability and high reproducibility, but also proposed a promising dual-signal amplification SERS-based diagnostic method for COVID-19, holding immense potential for the detection of a wide range of antigens and infectious diseases in future applications.
研究领域:
生物传感器
·
分析化学
·
病毒学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jie Shi,Xiaoqing Huang,Jiangtao Xu
Island-shaped Ag nanoparticles are prepared under the support platform of quartz fabric by a simple chemical deposition method. The prepared flexible SERS substrate in this study demonstrates highly sensitive, excellent signal reproducibility and stability. The enhancement ability can be adjusted by varying concentration of Ag + during synthesizing process, which would lead to the variation of size and gaps of Ag nanoparticles , thus affecting the LSPR effect. Moreover, the prepared SERS substrate shows great practical potential in rapid detection of contaminated fruit with different sampling method.
研究领域:
材料科学
·
分析化学
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DOI
高影响因子
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