JOURNAL OF LIGHTWAVE TECHNOLOGY
·
2026
·
IF 5.2
Tianli Huo,Lei Zhang,Xili Jing,Shuguang Li,Lu Chen,Dongfang Li
Glyceryl tributyrate is an ester compound that plays a crucial role in maintaining human intestinal health, immune regulation, and metabolic control. This study introduces a lipase-based specific self-assembled layer optical fiber SPR sensor, which achieves dual-parameter detection of glyceryl tributyrate and temperature for the first time. The assembly layer utilizes polyacrylic acid (PAA) and chitosan (CS) to immobilize lipase in the detection area of the sensor, achieving specific detection. MoS2 is employed to detect temperature, allowing for modulation and differentiation of the dual-channel operating wavelengths and enhancing the sensor's sensitivity. Initially, simulations were conducted using COMSOL to clarify the sensor's working principle. Subsequently, detection spectra under 0-6 layers were analyzed at detection concentrations ranging from 0.5 to 5 mmol, ultimately selecting 2 layers as the detection layer count. Then, single-channel temperature detection was performed on the temperature channel. Finally, the dual-channel sensor underwent cascade operation detection, along with stability and specificity tests. The results showed that at detection concentrations ranging from 0.5 to 5 mmol, the sensitivity of the concentration probe was 3.448 nm/mmol, with a detection limit of 0.32 mmol, meeting the millimeter-scale standards required for anticancer applications. The temperature probe exhibited a sensitivity of -2.43 nm/°C over the range of 0-100°C. Under dual-channel cascade operation, both detection spectra could operate independently, enabling simultaneous detection of temperature and glyceryl tributyrate. This sensor offers advantages such as high sensitivity, simplicity, and label-free measurement, providing a novel approach for simultaneous detection of glyceryl tributyrate and temperature.
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高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
·
2013
·Vol. 44
·期 8
·1120-1125
·
IF 2.679
Bi-Qi Zhang,Song-Bo Li,Qing Xiao,Ju Li,Jian-Jun Sun
The recently reported shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) is considered as the next generation of advanced spectroscopy for its surface and molecular generality. With the aim to utilize the virtues of shell-isolated strategy and advance the SHINERS technique, we introduce a silane-based rapid synthesis method of silica-coating Au nanorods (Au@SiO 2 NRs) with manoeuvrable ultra-thin shell and tunable SPR. The results demonstrate that the SPR of Au NRs could be optimized to obtain large Raman enhancement using either 633 nm or 785 nm laser. Differing from previously reported Au@SiO 2 NRs synthesis method, we can tune the silica shell thickness within several nanometers to maximize the Raman signal while effectively eliminating the exterior interference. And this advanced synthesis method has also significantly reduced the silica-coating time from one day to ca. 1 h. This method as a new development of SHINERS technique has successfully got enhanced signal in solution Raman tests of malachite green, giving a great potential to be extended to in-situ measurement for daily life detection. Copyright © 2013 John Wiley & Sons, Ltd.
研究领域:
材料科学
·
光谱学
·
纳米技术
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Hua Fang,Xiumei Wang,Mengyu Li,Mengxian Huang,Yiping Zhang,Taige Zhao,Cuiling Sun,Min Xu,Wenhui Jin,Weizhu Chen,Meijuan Fang
In this study, twenty-five novel carboxylic acid DHA-alkanolamine derivatives were synthesized by the reaction of cis -docosa-4,7,10,13,16,19-hexaenoic acid-alkanolamine (DHA-AA) with various carboxylic acids, including Naproxen (S1), Ibuprofen (S2), Ferulic acid (S3), Mefenamic acid (S4), and Aspirin (S5). Their molecular structures were characterized using 1 H/ 13 C NMR and HRMS techniques. To evaluate the anti-inflammatory activities of synthesized compounds, RAW264.7 macrophage cells pretreated with or without these compounds, were stimulated by LPS, and the levels of pro-inflammatory cytokines (NO, TNF-α, IL-1β, and IL-6) were then measured by ELISA, qPCR, and Western Blot. The cell-based assays showed that compound 11 had marked anti-inflammatory activity. In addition, the molecular docking study and SPR assay demonstrated that compound 11 exhibited good Nur77-binding affinity (K D = 3.61 × 10 −6 M). Moreover, compound 11 was found to inhibit NF-κB activation in a Nur77-dependent manner. Notably, compound 11 could attenuate LPS-induced inflammation in the mouse acute lung injury (ALI) model. In conclusion, these derivatives are potential Nur77-targeting anti-inflammatory agents against versatile inflammatory and autoimmune disorders.
研究领域:
药理学
·
炎症研究
·
药物化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ruihuan Zhao,Dongxiao Li,Nan Yin,Zhimin Guo,Dengchao Wang,Xin Yao
Dopamine (DA) plays vital role in diagnosis and treatment of neurodegenerative disease. It is of high clinical relevance for developing simple, sensitive and selective DA detection method. But the electrochemical detection of DA still faces the problem of insufficient sensitivity, and generally need the complex material synthesis process to realize the selective detection of DA. In this work, we established a simple electrochemical surface plasmon resonance sensor for DA detection based on its electropolymerization property. The main interfering molecules of uric acid and ascorbic acid cannot undergo electropolymerization, while DA can produce polydopamine precipitation on the surface of Au film. The formation of polydopamine precipitation could amplify the SPR signal, and ensures the high selectivity and sensitivity of DA detection. The linear range is 0.01–1000 nM and the detection limit is as low as 1.4 pM. At the same time, this sensor has high selectivity for DA detection even in the presence of 50000-fold higher concentration of ascorbic acid and uric acid. Finally, the sensor was successfully applied to determine DA content in urine samples and DA released from PC12 cells under the stimulation of K + , demonstrating great potential in clinical applications.
研究领域:
分析化学
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神经科学
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生物传感器
·
临床诊断
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高影响因子
OPTICS EXPRESS
·
2024
·Vol. 32
·期 19
·33330-33343
·
IF 3.2
Zhibing Zhang,Zhiyong Yin,Shuguang Li,Linchuan Hu
To achieve more accurate analysis and detection of changes in liquid parameters, we propose a dual-parameter surface plasmon resonance (SPR) sensor that can measure refractive index (RI) and pH simultaneously. In this paper, we compare and analyze the transmission spectrum when the SPR effect is excited by the cladding mode of a photonic crystal fiber (PCF) and the core mode of the no-core fiber. The results show that the SPR effect excited using the cladding mode is stronger and the sensor has better loss peaks, which is more conducive to realizing the detection of the external environment. Therefore, the sensor uses PCF as the substrate and utilizes a cascade composite film structure, and the area where a silver film is deposited on the surface of the PCF is selected as the RI sensing area. Another part of the PCF, after the silver film is deposited, uses a high-sensitivity polyacrylic acid/chitosan self-assembled nanofilm as the pH-sensitive film. Experimental results show that the maximum sensitivity is 4122.44 nm/RIU and -57.82 nm/pH when the RI range is between 1.333 and 1.385 and the pH range is between 3.11 and 8.63, respectively. In addition, we experimentally verified that the sensor had good stability and repeatability. The design of the sensor provides new possibilities for real-time monitoring and precise control, helping to advance scientific research and the development of industry.
研究领域:
光学传感
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材料科学
·
生物医学工程
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Pengqi Gong,Xuegang Li,Qiming Zhao,Xue Zhou,Yanan Zhang,Yong Zhao
In order to realize the one-to-one correspondence between outputs and inputs in the multiparameter detection field, a multiparameter DNA hybridization sensor with temperature and pH detection channels based on a fiber-based triple surface plasmon resonance (Triple-SPR) effect is demonstrated in this paper. Compared with previous works, the significant innovation of this paper is the independent temperature testing and pH testing channels implemented based on a single fiber optic structure, and the high-sensitivity DNA hybridization detection channel. Polydimethylsiloxane and the self-assembled materials formed by polyacrylic acid (PAA) and chitosan (CS) are used for the real-time detection of temperature and pH. With the help of these two materials, the instantaneous response of the Triple-SPR sensor to temperature and pH has been realized, and the response times (RT) are less than 1 s and 20 s, respectively. After the modification of probe DNA (pDNA), the selective detection of complementary DNA (cDNA) is achieved. The sensitivity and detection limit (DL) reaches 0.09 nm/(nmol/L) and 3.69 nmol/L, respectively. The experimental results demonstrate that an approximately one-to-one correspondence exists between inputs and outputs. The output signals can be used to provide feedback signals for achieving stable temperature and pH environments. The advantages of multi-parameter independent detection, high-sensitivity as well as fast response of the fiber-based Triple-SPR sensing technology are of great significance in the field of future multi-parameter sensing, compensated detection as well as real-time bio-monitoring.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Lijuan Huang,Tongtong Li,Yingjun Zhang,Xiaohong Sun,Yongan Wang,Zhiyong Nie
The detection of psychoactive substances is an important branch of modern analytical chemistry and has many legally and socially relevant implications. The use of a surface plasmon resonance (SPR)-based gene–nanoparticle system has emerged as a promising technique for the rapid and ultrasensitive detection of molecular species such as drugs of abuse in biofluids. However, the development of a viable screening tool for the detection of multiple classes of drugs in complex media is a considerable challenge because the existing techniques lack affinity toward certain species due to matrix interference. Our aim was to develop a simple optical sensor array for the classification of nine narcotic drugs in aqueous solution and human urine. The UV–vis spectra of DNA–gold nanoparticles in the presence of nine narcotic drugs (pentobarbital sodium, caffeine, morphine, remifentanil, fentanyl, ketamine, etomidate, carfentanil, and sulfentanyl) were distinctly different. Furthermore, the narcotic drugs present in aqueous solution and in human urine were classified correctly through partial least squares discriminant analysis (PLS-DA). Combination with a multi-sensor unit further improved the prediction accuracy of the PLS-DA models. The proposed method has potential for on-site drug detection and drug abuse screening.
研究领域:
分析化学
·
纳米技术
·
化学计量学
·
法医学
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PubMed
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Ruihuan Zhao,Nan Yin,Lixin Ma,Jie Zhang,Yangkai Luo,Zhimin Guo,Mengmei Fa,Dingding Yang,Dengchao Wang,Xin Yao
ATP is related to the occurrence of many diseases, its facile, sensitive and accurate determination in real samples is needed for disease screening and treatment. Although some methods realized the sensitive detection of ATP using the signal amplification of cyclic reactions or nanoparticles, complex experimental processes limit their applications. In this paper, we report a simple surface plasmon resonance (SPR) method based on a single stranded DNA probe. The probe has dual recognition sites of ATP and Ag+; namely the ATP aptamer located in the middle of the probe and five cytosine (C) at both ends of the probe. In the absence of ATP, the probe transforms into a rigid hairpin structure by forming C-Ag+-C structures and produces a large SPR signal. However, once the probe interacts with ATP, three-dimensional structures are formed, and then the Ag+-induced probe configuration change is smaller, resulting in an small SPR signal. The signal difference before and after the probe interacts with ATP is proportional to the analyte concentration. The probe configuration change is in the optimum area for SPR detection, ensuring the sensitive ATP determination. The signal difference has a linear response with the logarithm of ATP concentration from 0.05 to 500 nM with a detection limit of 15 pM. Moreover, the strategy was demonstrated to determine ATP in serum samples with satisfactory recoveries. The results show that this simple and sensitive method has potential applications for clinical ATP determination.
研究领域:
生物化学
·
医学诊断
·
纳米技术
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Heng Shi,Zhiyi Liu,Xiaoxiao Wang,Jun Guo,Le Liu,Lin Luo,Jihua Guo,Hui Ma,Shuqing Sun,Yonghong He
In this work, we propose a MgF 2 /Au/MgF 2 /Analyte based, angle-interrogation surface plasmon resonance sensor. This symmetrical structure results in higher surface electric field strengths , and longer surface propagation lengths and depths. A refractive index resolution of 8.1 × 10 −8 RIU in fluid protocol and 3.5 × 10 −7 RIU in atmosphere protocol is acquired by a simple SPR imaging system. In order to demonstrate the biomedical applications of this system, we detect Escherichia coli with different concentrations using dopamine film on the sensor surface to immobilize antibodies. A detection limit of 10 3 cfu/mL of E. coli bacteria is acquired from this system.
研究领域:
光学
·
生物传感
·
纳米技术
·
生物医学工程
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高影响因子
ANALYST
·
2015
·Vol. 140
·期 13
·4566-4575
·
IF 4.107
Jacky F. C. Loo,S. S. Wang,F. Peng,J. A. He,L. He,Y. C. Guo,D. Y. Gu,H. C. Kwok,S. Y. Wu,H. P. Ho,W. D. Xie,Y. H. Shao,S. K. Kong
As in all RNA viruses, influenza viruses change and mutate constantly because their RNA polymerase has no proofreading ability. This poses a serious threat to public health nowadays. In addition, traditional pathogen-based detection methods may not be able to report an infection from an unknown type or a subtype of virus if its nucleotide sequence is not known. Because of these factors, targeting host microRNA signatures may be an alternative to classify infections and distinguish types of pathogens as microRNAs are produced in humans shortly after infection. Although this approach is in its infant stage, there is an urgent need to develop a rapid reporter assay for microRNA for disease control and prevention. As a proof of concept, we report herein for the first time a non-PCR MARS (MicroRNA-RNase-SPR) assay to detect the microRNA miR-29a-3p from human subjects infected with influenza virus H1N1 by surface plasmon resonance (SPR). In our MARS assay, RNase H is employed to specifically hydrolyze the RNA probes immobilized on the gold surface where they hybridize with its cognate target cDNAs miR-29a-3p, where it was formed from reverse transcription with mature miR-29a-3p specific stem-looped primers. After the digestion of the RNA probe by RNase H, the intact cDNA was released from the RNA–DNA hybrid and bound to a new RNA probe for another enzymatic reaction cycle to amplify signals. With assay optimization, the detection limit of our MARS assay for miR-29a-3p was found to be 1 nM, and this new assay could be completed within 1 hour without thermal cycling. This non-PCR assay with high selectivity for mature microRNA provides a new platform for rapid disease diagnosis, quarantine and disease control.
研究领域:
病毒学
·
分子生物学
·
诊断学
·
公共卫生
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Huang Yu,Pu Xiaoyin,Qian Husun,Chuang Chin-Jung,Dong Shanshan,Wu Jiangling,Xue Jianjiang,Cheng Wei,Ding Shijia,Li Shengqiang
This paper describes the simple and label-free detection of thrombin using optical fiber surface plasmon resonance (SPR) sensors based on gold films prepared by the cost-effective method of electroless plating. The plating conditions for simultaneously obtaining gold film on cylindrical core and end surfaces of an optical fiber suitable for measurement were optimized. The fabricated sensor exhibited a linear refractive index sensitivity of 2150 nm/RIU and 7.136 (a.u.)/RIU in the refractive index of 1.3329–1.3605 interrogated by resonance wavelength and amplitude methods respectively and a single wavelength monitoring method was proposed to investigate the sensing performance of this sensor. Polyadenine diblock and thiolated thrombin aptamers were immobilized on gold nanoparticles and gold films respectively to implement a sandwich optical fiber assay for thrombin. The developed optical fiber SPR sensors were successfully used in the determination of thrombin down to 0.56 nM over a wide range from 2 to 100 nM and showed good selectivity for thrombin, which indicated their potential clinical applications for biomedical samples. Graphical Abstract
研究领域:
光学
·
生物传感器
·
分析化学
·
生物医学工程
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PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2020
·Vol. 117
·期 30
·17564-17570
·
IF 9.412
Hui Wang,Hui Yu,Yan Wang,Xiaonan Shan,Hong-Yuan Chen,Nongjian Tao
When a metal nanoparticle is brought near to a metal surface within electron tunneling distance (∼1 nm), classical electromagnetic coupling between the nanoparticle and the metal is expected to transition to quantum coupling. We show that this transition can be observed as a drastic phase change in the surface plasmon resonance (SPR) images of the gold nanoparticles. We study the transition by controlling the distance between the nanoparticles and electrode surface, modeling the impact of the transition on the SPR image in terms of a phase shift and demonstrating detection of microRNA based on the transition from classical to quantum coupling. The work shows that the quantum coupling can be directly visualized in SPR, and the extremely sensitive dependence of the transition on distance leads to a biosensing principle with SPR.
研究领域:
纳米光子学
·
等离子体学
·
生物传感
·
量子电动力学
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PubMed
高影响因子
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