TALANTA
·
2019
·
IF 4.916
Yanli Shi,Honglin Yang,Yong He,Zhifeng Fu
Bacterial analysis and antibiotic resistance testing (ART) are of great significance in clinical diagnosis and therapy of bacterial infectious diseases. In this work, a portable antibiotic-affinity chromatographic test strip has been developed for rapid analysis of Staphylococcus aureus ( S. aureus ) and further applied for ART of this pathogen. Porcine IgG was immobilized on a nitrocellulose membrane for capturing S. aureus based on the selective binding capability of the Fc fragment of IgG toward protein A on the surface of the target bacteria. Fluorescent microspheres modified with teicoplanin (TEI) were applied as signal substances to trace S. aureus utilizing the hydrogen bond conjugation between this antibiotic and Gram-positive bacteria. S. aureus can be analyzed within the concentration range from 1.4 × 10 3 CFU mL −1 to 1.4 × 10 7 CFU mL −1 . The recovery values for spiked samples were 93.3–110.0%. The obtained results of ART for penicillin, daptomycin , gentamicin, cefoxitin and clindamycin against S. aureus showed agreement with those of traditional broth dilution method. The procedures for bacterial analysis and ART can be accomplished within 20 and 110 min, respectively. The antibiotic-affinity chromatographic test strip showed great promise in point-of-care testing because of its ideal portability and rapidity.
研究领域:
临床微生物学
·
分析化学
·
生物技术
·
医疗器械
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Mengyao Wang,Honglin Yang,Yue Wu,Zhifeng Fu
A dual sites affinity protocol was developed for fluorescent analysis of Staphylococcus aureus ( S. aureus ) by employing daptomycin and immunoglobulin G (IgG) as the recognition elements . Pig IgG immobilized on microplate was employed as the first recognition element to capture S. aureus owing to the fact that the Fc segment of mammal IgG can selectively bind with protein A on the surface of the target bacteria. Meanwhile, fluorescein isothiocyanate-conjugated daptomycin was employed as the second recognition element as well as the signal tracer for the target bacteria utilizing the binding capability of daptomycin to Gram-positive bacteria. S. aureus can be analyzed within a concentration range of 5.0 × 10 3 –5.0 × 10 8 CFU mL −1 with a detection limit of 3.6 × 10 3 CFU mL −1 . The analytical process can be accomplished within 1.5 h by using a pre-coated microplate. The dual sites affinity protocol can exclude the interference led by Gram-negative bacteria and other common Gram-positive bacteria. We have successfully applied it to analyze S. aureus in spiked lake water and physiological saline injection samples, and the recovery values ranged from 88.0% to 120.0%. The results demonstrate its application potential for environmental sanitation and drug safety control.
研究领域:
微生物学
·
分析化学
·
环境科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 10
·1503
·
IF 5.561
Ying Feng,Tinghong Ming,Jun Zhou,Chenyang Lu,Rixin Wang,Xiurong Su
Staphylococcus aureus(S. aureus) has a strong tolerance to high salt stress. It is a major reason as to why the contamination ofS. aureusin salted food cannot be eradicated. To elucidate its response and survival mechanisms, changes in the morphology, biofilm formation, virulence, transcriptome, and metabolome ofS. aureuswere investigated.IsaApositively regulates and participates in the formation of biofilm. Virulence was downregulated to reduce the depletion of nonessential cellular functions. Inositol phosphate metabolism was downregulated to reduce the conversion of functional molecules. The MtsABC transport system was downregulated to reduce ion transport and signaling. Aminoacyl-tRNA biosynthesis was upregulated to improve cellular homeostasis. The betaine biosynthesis pathway was upregulated to protect the active structure of proteins and nucleic acids. Within a 10% NaCl concentration, the L-proline content was upregulated to increase osmotic stability. In addition, 20 hub genes were identified through an interaction analysis. The findings provide theoretical support for the prevention and control of salt-tolerant bacteria in salted foods.Keywords:Staphylococcus aureus;salted foods;salt stress
研究领域:
微生物学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Xia Liu,Zhen Lei,Dianjun Liu,Zhenxin Wang
It still confronts an outstanding challenge to screen efficient antibacterial drugs from millions of potential antibiotic candidates. In this regard, a sandwiched microarray platform has been developed to culture live bacteria and carry out high-throughput screening antibacterial drugs. The optimized lectin-hydrogel microarray can be used as an efficient bacterial capturing and culturing platform, which is beneficial to identify spots and collect data. At the same time, a matching drug-laden polyacrylamide microarray with Luria–Bertani (LB) culture medium can be generated automatically and accurately by using a standard non-contacting procedure. A large number of microscale culture chambers (more than 100 individual samples) between two microarrays can be formed by linking two aligned hydrogel spots using LB culture medium, where live bacteria can be co-cultured with drug candidates. Using Staphylococcus aureus ( S. aureus ) and four well-known antibiotics (amoxicillin, vancomycin , streptomycin and chloramphenicol) as model system, the MIC (minimum inhibitory concentration) values of the antibiotics can be determined by the drug induced change of bacterial growth, and the results demonstrate that the MIC values of amoxicillin , vancomycin and streptomycin are 1.7 μg mL −1 , 3.3 μg mL −1 and 10.3 μg mL −1 , respectively.
研究领域:
微生物学
·
药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Zhengzheng Wang,Ju Liu,Guanhua Chen,Xiaoyan Feng,Mei Deng,Dan Mu,Qian Xu,Hengyi Xu
Staphylococcus aureus ( S. aureus ) is a common pathogen that can cause severe foodborne diseases . Therefore, the detection of S. aureus in food has been the focus of attention. In this study, a strategy for the detection of S. aureus using magnetic separation technology combined with colorimetry was proposed. Phenylboronic acid, as a compound that had been widely used in the identification of sugars and sugar-based molecules, can selectively enrich compounds with cis -diol groups through a reversible esterification reaction. Hence, 3-aminophenylboronic acid (APBA) could be used as a recognition molecule to capture bacteria, because there were a large amount of peptidoglycan and lipopolysaccharide on the surface of Gram-positive bacteria and Gram-negative bacteria. At the same time, there are few studies on the use of APBA as magnetic separation recognition molecule to enrich pathogenic bacteria. Therefore, we prepared APBA-functionalized magnetic beads (APBA-MBs) for pre-enrichment of S. aureus and an APBA-MBs based untargeted magnetic separation was constructed to replace the traditional targeted magnetic separation. In order to realize the output signal and guarantee the specificity for detecting S. aureus , we used HRP-pig IgG because the Fc fragment of IgG could bind the protein A on the cell wall of S. aureus . Finally, the tetramethylbenzidine (TMB) color development solution was occurred the chromogenic reaction under the action of the HRP enzyme. Under the optimal experimental conditions, the limit of detection (LOD) of the APBA-MBs combined with color reaction was 3.0 × 10 2 CFU/mL in pure culture, fruit juice, pool water and spinach.
研究领域:
食品安全
·
微生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Jie Shi,Yazhu Wang,Huanran Wei,Jiajun Hu,Min-Tian Gao
To reduce the production cost of bioethanol and improve the utilization rate of straw resources, more high value-added products have to be found and applied. In this study, tannins were considered as a high-value by-product. The extraction of condensed tannins from rice straw was studied, and the tannins were characterized. The crude tannins consisted mainly of tannins, phenolic acids and monosaccharides. The crude tannins were separated and purified with AB-8 macroporous resin and Sephadex LH-20. The matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) assay confirmed that catechin, epicatechin, epigallocatechin, epicatechin gallate and gallic acid were the major components in the purified tannins. The tannins exhibited strong inhibitory effects on growth of Staphylococcus aureus . Higher concentrations of crude tannins resulted in lower ethanol concentration and intercellular ATP content, and higher residual glucose. Moreover, the tannins could regulate morphology of biofilms. The images from atomic force microscopy and confocal laser scanning microscopy showed that cells hardly adhered to the plate surface, and large aggregations of cells were observed in culture medium containing tannins, and almost all cells were dead. This study provides the feasibility of tannins derived from rice straw as new antimicrobial agents with a different inhibitory mechanism.
研究领域:
生物化学
·
微生物学
·
生物技术
·
药学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Lin Yue,Meihong Zheng,Min Wang,Imran Mahmood Khan,Xiaowei Ding,Yin Zhang,Zhouping Wang
The development of new antimicrobial agents is important to combat infections caused by pathogenic bacteria. Herein, Hydroxypropyl chitosan (HPCS), a hydrophilic modified product of chitosan (CS), was employed as a carrier of the photosensitizer chlorin e6 (Ce6) through an amide bond to obtain the products (HPCS-Ce6 conjugates) with a degree of substitution (DS) ranging from 2.95% to 5.25%. The UV–vis absorption spectra and 1 H NMR spectra confirmed the successful synthesis of the products. The products have a better and more stable reactive oxygen species (ROS) generation capacity and higher bacterial affinity than Ce6. At a very low dose (1.8 μg/mL), the highest DS product (HPCS-Ce6-3) can effectively kill Staphylococcus aureus ( S. aureus ) under 660 nm irradiation. In addition, the HPCS-Ce6 conjugates showed high biocompatibility in the CCK-8 test. The HPCS-Ce6 conjugates could be a photodynamic antibacterial agent with good water solubility, high biocompatibility, and antibacterial activity .
研究领域:
生物化学
·
微生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Shumin You,Qiaoyin Li,Haiyan Chen,Zhenyu Lin,Shenghang Zhang,Xiaohua Jiang,Bin Qiu
Staphylococcus aureus(S. aureus) can cause a variety of diseases,so it is essential to provide the simple and responsive identification of bacterial pathogens in clinical treatment. In this work a homogeneous aptamer electrochemiluminescence biosensor for Staphylococcus aureus was developed assisted based on hybridization chain reaction and magnetic separation assistance. The ds (Probe DNA/Aptamer) is modified on the magnetic beads. In the presence of the target the target binds to the Aptamer in ds (Probe DNA/Aptamer) exposing the Probe DNA in the ds DNA. The exposed Probe DNA triggers HCR resulting in long DNA duplexes which can be simply and efficiently enriched for the luminescent molecule Ru(phen) 3 2+ . To reduce the possibility of false positive signals Exo III was added to cut unreacted dsDNA from the magnetic beads. The ECL intensity was linearly correlated with the logarithm of S. aureus concentration in the range of 10 CFU/mL − 10 × 10 7 CFU/mL with a detection limit of 3 CFU/mL. The method developed in this work has good sensitivity and specificity and can well evaluate the concentration of Staphylococcus aureus in normal human serum and the results are consistent with the results obtained by the plate counting method.
研究领域:
生物传感器
·
分析化学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2022
·Vol. 19
·期 3
·2204039
·
IF 15.153
Jing Sun,Xutao Ma,Rongye Li,Min Lin,Lilei Shu,Xuesi Chen
Self-assembled cationic polymeric nanostructures have been receiving increasing attention for efficient antibacterial agents. In this work, a new type of antibacterial agents is developed by preparing pH-dependent nanostructured assemblies from cationic copolypeptoid poly( N -allylglycine)- b -poly( N -octylglycine) (PNAG- b -PNOG) modified with cysteamine hydrochloride ((PNAG- g -NH 2 )- b -PNOG) driven by crystallization and hydrophobicity of the PNOG blocks. Due to the presence of confined domains arising from crystalline PNOG, persistent spheres and fiber-like assemblies are obtained from the same polymer upon a heating-cooling cycle. This allows for direct comparison of antimicrobial efficiency of nanostructured assemblies with various morphologies that are otherwise similar. Both nanostructured assemblies exhibit extremely low toxicity to human red blood cells, irrespective of the presence of the hydrophobic block. Enhanced antimicrobial performance of the fiber-like micelles compared to the spheres, which result in high selectivity of the fibers, is shown. Notably, the fiber-like micelles show great efficacy in inhibition of the Staphylococcus aureus ( S. aureus ) biofilm formations and eradication of the mature biofilms, superior to vancomycin. The micelles also show potent in vivo antimicrobial efficacy in a S. aureus infection mouse skin model. With a systematic study, it is demonstrated that both micelles kill the bacteria through a membrane disruption mechanism. These results imply great potential of polypeptoid assemblies as promising excellent candidates for antibacterial treatment and open up new possibilities for the preparation of a new generation of nanostructured antimicrobials.
研究领域:
生物材料学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Ting Mao,Bao Chai,Yanpeng Xiong,Hongyan Wang,Lei Nie,Renhai Peng,Peiyu Li,Zhijian Yu,Fang Fang,Xianqiong Gong
Biofilm or persister cells formed by Staphylococcus aureus are closely related to pathogenicity. However, no antimicrobials exist to inhibit biofilm formation or persister cells induced by S. aureus in clinical practice. This study found that pinaverium bromide had antibacterial activity against S. aureus, with the MIC50/MIC90 at 12.5/25 μM, respectively. Pinaverium bromide (at 4 × MIC) showed a rapid bactericidal effect on S. aureus planktonic cells, and it was more effective (at least 1-log10 cfu/mL) than linezolid, vancomycin, and ampicillin at 4 h of the time-killing test. Pinaverium bromide (at 10 × MIC) significantly inhibited the formation of S. aureus persister cells (at least 3-log10 cfu/mL) than linezolid, vancomycin, and ampicillin at 24, 48, 72, 96, and 120 h of the time-killing test. Biofilm formation and adherent cells of S. aureus isolates were significantly inhibited by pinaverium bromide (at 1/2 or 1/4 × MICs). The fluorescence intensity of the membrane polarity of S. aureus increased with the treatment of pinaverium bromide (≥1 × MIC), and the MICs of pinaverium bromide increased by 4 times with the addition of cell membrane phospholipids, phosphatidyl glycerol and cardiolipin. The cell viabilities of human hepatocellular carcinoma cells HepG2 and Huh7, mouse monocyte-macrophage cells J774, and human hepatic stellate cells LX-2 were slightly inhibited by pinaverium bromide (<50 μM). There were 54 different abundance proteins detected in the pinaverium bromide-treated S. aureus isolate by proteomics analysis, of which 33 proteins increased, whereas 21 proteins decreased. The abundance of superoxide dismutase sodM and ica locus proteins icaA and icaB decreased. While the abundance of global transcriptional regulator spxA and Gamma-hemolysin component B increased. In conclusion, pinaverium bromide had an antibacterial effect on S. aureus and significantly inhibited the formation of biofilm and persister cells of S. aureus.
研究领域:
微生物学
·
药理学
·
感染病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Carbon Letters
·
2023
·
IF 4.5
Li Guochun,Li Jiaxin,Yang Ziyu
This study reports the synthesis of a novel graphene/chitosan/β-cyclodextrin composite material (GO/CS/β-CD) via a one-step chemical reduction method, which combines the advantages of graphene, chitosan, and β-cyclodextrin. The morphology and structure of the composite were characterized using various techniques, such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. Subsequently, sortase A (SA) was immobilized onto the GO/CS/β-CD for the detection of Staphylococcus aureus . The sensor exhibited a good linear relationship within the concentration range of 30–300 CFU/mL, with a detection limit of 12 CFU/mL. The GO/CS/β-CD composite material showed enhanced properties due to the synergistic effect of graphene, chitosan, and β-cyclodextrin. The immobilization of sortase A onto the composite material improved the sensitivity and selectivity of the sensor for the detection of S. aureus . This study presents a novel graphene/chitosan/β-cyclodextrin composite material with immobilized sortase A, demonstrating enhanced sensitivity and selectivity for the detection of Staphylococcus aureus, which has potential for the development of high-performance sensors in various fields.
研究领域:
生物传感
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 6.7
Tao Li,Xiaoli He,Wenlan Tao,Ruixue Zhang,Qiaolin He,Hongzhi Gong,Ye Liu,Dong Luo,Maojie Zhang,Cheng Zou,Shao-Lin Zhang,Yun He
Infections caused by drug-resistant bacteria have become a new challenge in infection treatment, gravely endangering public health. Chloramphenicol (CL) is a well-known antibiotic which has lost its efficacy due to bacterial resistance . To address this issue, herein we report the design, synthesis and biological evaluations of novel triphenylphosphonium chloramphenicol conjugates (TPP + -CL). Study results indicated that compounds 39 and 42 possessed remarkable antibacterial effects against clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) with MIC values ranging from 1 to 2 μg/mL, while CL was inactive to the tested MRSA strains. In addition, these conjugates exhibited rapid bactericidal properties and low toxicity, and did not readily induced bacterial resistance , obviously outperforming the parent drug CL. In a mouse model infected with a clinically isolated MRSA strain, compound 39 at a dose of 20 mg/kg exhibited a comparable or even better in vivo anti-MRSA efficacy than the golden standard drug vancomycin , while no toxicity was observed.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）