JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Jun Cheng,Zheyu Li,Xian Bao,Ruijun Zhang,Zhiqiang Zhang,Guangtong Hai,Kai Sun,Wenxin Shi
An urgent challenge during the preparation of polyamide thin film composite membrane via the interfacial polymerization method was excessive diffusion rate of piperazine to form uncontrollable polyamide layer thickness, ultimately leaded to low water permeability to increase the operating cost. Herein, a uniquely new method, which brought down one order of magnitude of piperazine concentration to ultra-low grade (0.01% w·v −1 ) to retard the diffusion rate, was utilized to avoid the problem. However, the ultra-low concentration of piperazine could not easily react with trimesoyl chloride, bis-tris propane as conditioning agent was needed to overcame the trouble. The kinetics diffusion process of piperazine under the function of bis-tris propane was intensively studied by molecular dynamics simulation and other characterizations. Bis-tris propane could combine with piperazine to generate intermolecular force and then carry piperazine molecules moving toward the interface of aqueous/organic phase, which tuned the trans -state of piperazine to the benefit of rection. This work offered a novelty insight to surmount the problem of the excessive diffusion rate, which could promote the development of membrane technology .
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高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Shimeles Addisu Kitte,Fuad Abduro Bushira,Tesfaye Refera Soreta
Efficient coreactants are of high importance in electrochemiluminescence sensing. Herein, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide was demonstrated as an effective coreactant for tris(2,2′-bipyridine)ruthenium(II) electrochemiluminescence system for the first time. The new electrochemiluminescence system showed a good sensing performance for H 2 O 2 on the basis of quenching of the electrochemiluminescence intensity. It allows the detection of H 2 O 2 in the dynamic range of 0.5–200 µM. The limit of detections for H 2 O 2 was 0.33 µM ( 3σ/m ). In addition, the developed ECL system exhibited satisfactory recoveries for H 2 O 2 sensing in real samples.
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高影响因子
ENVIRONMENTAL POLLUTION
·
2016
·
IF 4.839
Jing Li,Xufa Ma,Guanyong Su,John P. Giesy,Yuan Xiao,Bingsheng Zhou,Robert J. Letcher,Chunsheng Liu
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is considered a re-emerging environmental pollutant, and exposure to environmentally relevant concentrations has been shown to cause individual developmental toxicity in zebrafish and the water flea ( Daphnia magna ). However, multigenerational effects during exposure to TDCIPP and after subsequent recovery were unknown. In the present study, individuals of a model aquatic organism, the ciliated protozoan, T. thermophila were exposed to environmentally-relevant concentrations of TDCIPP (0, 300 or 3000 ng/L) for 60 days (e.g., theoretically 372 generations) followed by a 60-day period of recovery, during which T. thermophila were not exposed to TDCIPP. During exposure and after exposure, effects at the molecular, histological, individual and population levels were examined. Multigenerational exposure to 300 or 3000 ng TDCIPP/L for 60 days significantly decreased numbers of individuals, sizes of individuals, expressed as length and width of bodies, number of cilia, and depth and diameter of basal bodies of cilia, and up-regulated expressions of genes related to assembly and maintenance of cilia. Complete or partial recoveries of theoretical sizes of populations as well as sizes of individuals and expressions of genes were observed during the 60-day recovery period. Effects on number of cilia and depth and diameter of basal body of cilia were not reversible and could still be observed long after cease of TDCIPP exposure. Collectedly, and shown for the first time, multigenerational effects to T. thermophila were caused by exposure to environmentally relevant concentrations of TDCIPP. Also, there were multi-generational effects at the population level that were not caused by carry-over exposure to TDCIPP. The “permanent” alterations and their potential significance are discussed.
研究领域:
环境毒理学
·
分子生物学
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高影响因子
POLYMER
·
2010
·
IF 3.573
Jianbo Li,Jie Ren,Yang Cao,Weizhong Yuan
Biodegradable star-shaped poly( ɛ -caprolactone) and poly( ɛ -caprolactone- b - l -lactide) (5sPCL- b -PLLA) with five arms were synthesized by ring-opening polymerization (ROP) from an asymmetric BIS-TRIS core via “core-first” strategy. Subsequently, a series of amphiphilic and double responsive star-block copolymers were synthesized by Z -RAFT star polymerization of N , N -dimethylamino-2-ethyl methacrylate (DMAEMA) from the star-shaped macro-RAFT agent, which was prepared by attaching 3-benzylsulfanylthiocarbonylthiocarbonylsufanylpropionic acid (BSPA) to 5sPCL- b -PLLA using a simple two-step reaction sequence. GPC and 1 H NMR data demonstrated the polymerization courses are under control. The molecular weight of 5sPCL- b -PLLA- b -DMAEMA increased with the monomer conversion, and the molecular weight distribution was in the range of 1.19–1.37. The spherical micelles with degradable core and pH/thermo-double sensitive shell had been prepared from the aqueous medium of the amphiphilic star-shaped copolymers by dialysis method. Both pH and thermal-responsive behaviours of copolymer micelles obtained in this study were investigated. The micelle size and morphology were measured by DLS, AFM and TEM .
研究领域:
高分子化学
·
材料科学
·
纳米医学
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 39
·期 9
·1700-1708
·
IF 2.741
Na Wang,Hui Yu,Shijun Shao
This paper describes the use of graphene oxide/silica modified with nitro-substituted tris(indolyl)methane as a solid-phase extraction sorbent for the determination of organic acids. The resultant graphene oxide/silica modified with nitro-substituted tris(indolyl)methane was characterized by FTIR spectroscopy and adsorption experiments. Solid-phase extraction parameters such as sorbent type, sample solution pH, sample loading rate, eluent salt concentration, eluent methanol concentration, elution rate, sample loading, and elution volume were optimized. The method showed good precision, accuracy, sensitivity, and linear response for organic acids analysis over a concentration range of 1–100 μg/L for benzoic acid, p -methoxybenzoic acid, and salicylic acid and 5–100 μg/L for the remaining organic acids (cinnamic acid, p -chlorobenzoic acid, and p -bromobenzoic acid) with coefficients of determination ( r 2 ) of higher than 0.9957. Limits of detection from 0.50 to 1.0 μg/L for six organic acids were achieved. The developed method was successfully applied to determine organic acids in real samples.
研究领域:
分析化学
·
环境科学
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高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Muhammad Saqib,Shahida Bashir,Haijuan Li,Chuanping Li,Shanshan Wang,Yongdong Jin
Developing rapid, specific, and low-cost electrogenerated chemiluminescence (ECL) systems remains a compelling goal for many applications including environmental analysis, clinical diagnostics, and food safety. In this study, N-hydroxysuccinimide (NHS) esters, especially N-hydroxysulfosuccinimide (NHSS), was exploited for the first time as an efficient coreactant for anodic Ru(bpy)32+ ECL in neutral medium. Among the three tested NHS esters (NHS, NHSS, NHPI), the NHSS exhibited much higher ECL intensity and stability than the sulfonate-free esters, suggesting the essential role of both the N-hydroxy (NOH) and sulfonate (SO3H) moieties in the developed Ru(bpy)32+ ECL system. The ECL generation process is rather straightforward, not requiring time-consuming fabrication/modification steps of electrodes. The anodic Ru(bpy)32+–NHSS system achieved ECL detections with high selectivity and sensitivity toward l-proline (LOD = 50 nM) and Hg2+ (LOD = 10 nM), with a linear range of 0.5–200 μM and 0.1–25 μM, respectively. The method showed good recoveries (95.2–104.8%) for l-proline and Hg2+ detection in real samples. This study is a successful step toward the development of new coreactants and may open new avenues for the applications of N-hydroxy compounds in ECL sensing.
研究领域:
分析化学
·
环境科学
·
临床诊断
·
食品安全
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Jian Yannan,Wang He,Lan Feifei,Liang Linlin,Ren Na,Liu Haiyun,Ge Shenguang,Yu Jinghua
An electrochemiluminescence (ECL) biosensor is described for the detection of microRNA (miRNA-155) based on tris(bipyridine)ruthenium(II) functionalized metal organic framework (RuMOF) materials. The material was prepared by a solvothermal method and was found to be stable even in acidic solution. However, it is selectively and sensitively disassembled by Hg(II) ions, resulting in the release of large quantities of Ru(II)(bpy) 3 ions, which produces a strong ECL signal. In view of the ion-selective disassembly and release and strand displacement process, an ultrasensitive ECL sensing method was established for detection of microRNAs. In the presence of the target, the hairpin structure of H1 can open and hybridize with the hairpin probe H2 to form a more stable H1-H2 duplex structure than the H1-target hybrid. The target of hybridization to H1 was immediately freed from the structure and the released target re-entered the new hairpin assembly target recovery process. The remaining H2 single fragment can bind to the I-RuMOFs-conjugates. The more hairpin probes H1, the more I-RuMOFs-conjugates load the DNA fragments, leading to the signal amplification. The method works in the 0.8 f. to 1.0 nM miRNA-155 concentration range and has a detection limit of 0.3 fM. The assay is sensitive, fairly specific and remarkably stable. In our perception, it offers an attractive tool for the sensitive detection of microRNAs in clinical samples.
An electrochemiluminescence (ECL) based biosensor is described for the detection of microRNA (miRNA-155) based on the use of a metal organic framework functionalized with ruthenium(II)tris(bipyridine) that was deposited on a glassy carbon electrode (GCE) modified with gold nanoparticles.
研究领域:
生物传感技术
·
分子诊断学
·
分析化学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Zheng Chang,Qiqi Zhao,Binghua Yao,Lei Han
Novel electroactive nanotags of Cu(II) functionalized polyethylenimine (PEI)/SiO2 nanoparticles (abbreviated as Cu2+/PEI/SiO2 NPs) were conveniently synthesized using a strong coordination reaction between Cu(II) and the multi-amino groups of PEI. These electroactive nanotags were easily coated on the overoxidized polypyrrole (OPPy) modified Nafion/multi-walled carbon nanotubes (abbreviated as Nafion/MWNTs) electrode surface through Cu-N bonds and strong electrostatic interactions. The Cu2+/PEI/SiO2 NPs@OPPy/MWNTs hybrid nanomaterial was formed without the addition of extra membrane-forming substances. The experimental results demonstrate that the OPPy/MWNTs composite membrane coupled with electrocatalytic Cu(Tris)42+ complexes in tris(hydroxymethyl)aminomethane (Tris)-HCl buffer medium amplified the electrochemical signals of nanotags based on the mutual synergistic effects. As an application, a label-free electrochemical immunosensor for epithelial ovarian cancer biomarker-carbohydrate antigen-125 (CA125) was constructed by covalently immobilizing CA125 antibody onto the hybrid nanomaterial modified electrode. With the increasing concentrations of CA125 antigen, different decreases in the electrochemical signals of the nanotags were observed due to the formation of antigen-antibody immune complexes. The electrochemical immunosensor showed a linear relationship from 0.001 to 500 U·mL−1 for CA125 with a detection limit of 3.4 × 10−4 U·mL−1. In addition, the proposed immunosensor was applied to the determination of CA125 in human serum with satisfactory results.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
癌症生物学
·
电化学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wu Lijuan,Li Jiayin,Yang Yin,Ju Kaize,Jia Ziyao,Gao Biqiang,Jin Shiwei,Sun Qun,Wu Laiyan
This study investigated the interactions between pepsin (PEP) and the organophosphite antioxidant Irgafos 168 (I 168), its metabolite 2,4-di-tert-butylphenol (24DP), focusing on structural, functional, and antioxidant implications. UV–Vis absorption spectra, fluorescence lifetimes and Stern-Volmer quenching analyses show that I 168/24DP statically bursts PEP with high binding affinity. Thermodynamic parameters, molecular docking analyses and molecular dynamics simulations (e.g. RMSD, Rg) indicate that the spontaneous formation of the stable complexes is mainly driven by van der Waals forces and hydrophobic interactions. I 168 exhibited higher pepsin inhibition (6.97 %–27.51 %) than 24DP (5.04 %–19.29 %), with dose-dependent suppression linked to altered catalytic residue accessibility (e.g., Asp32/Asp215, Tyr75) and disrupted proton transfer efficiency. Structural analyses revealed I 168 increased PEP's flexibility and hydrophobic residue exposure, while 24DP enhanced rigidity and compactness. Antioxidant assays showed 24DP's superior DPPH scavenging capacity (9.45 %–47.35 %) compared to I 168 (2.28 %–8.00 %), though PEP significantly suppressed 24DP's antioxidant efficacy. These findings underscore the necessity of evaluating both parent compounds and metabolites in safety assessments, as metabolic processes retained biological impacts on enzymatic and antioxidant functions. The study provides critical insights into the molecular mechanisms of antioxidant-induced enzyme inhibition and highlights risks to digestive health and food safety.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Jinzhi Ren,Ying Peng,Luhang She,Lu Yan,Jianjun Li,Caixia Gao,Chao Wang,Yimeng Wang,Xiangping Nie,Xiaowei Zhang
The estuarine ecological environment faces significant threats from contaminants of emerging concern (CECs); yet, the risk posed by CECs to resident organisms remains poorly understood. Here, we employed tiered toxicity testing to investigate the adverse effects and potential mechanisms of tris (1-chloro-2-propyl) phosphate (TCPP) on the early life stages of an estuarine fish, Mugilogobius chulae . TCPP affected the development of M. chulae embryos, including survival, morphology, hatching, and behavior. A concentration-dependent transcriptomic analysis showed that TCPP disrupted 12 neurodevelopment-related KEGG pathways in M. chulae embryos, with five of the 30 % top-ranked pathways related to neurotransmitter signaling. Besides the cholinergic synapse signaling pathway, the glutamatergic signaling pathway (including NMDAR and AMPAR subtypes) may also mediate TCPP-induced neurodevelopmental toxicity. The NMDAR subtype GRIN2B was downregulated at high concentrations. Molecular dynamics simulations revealed a strong interaction between TCPP and GRIN2B, with TCPP binding to the residues Ile153 and Ile188. The results suggest that NMDARs play a crucial role in TCPP-induced neurodevelopmental toxicity toward M. chulae. AOP network analysis predicted that TCPP may impact cognitive functions and memory. Our study provides a novel testing strategy for identifying the mechanisms of toxicity of CECs, a crucial component of ecological risk assessment.
研究领域:
环境毒理学
·
水生生态学
·
分子生物学
·
神经科学
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DOI
PubMed
高影响因子
ELECTROCHEMISTRY
·
2015
·Vol. 83
·期 3
·155-160
·
IF 1.033
Yan-qing LI,Ying LUO,Li-jun LI,Hao CHENG,Wen-yi HUANG
In order to establish a simple, sensitive and rapid method for the determination of azithromycin, the gold electrode modified with Silica sol/Nano-Au/PVP/Ru(bpy)32+ was prepared, and the electrochemiluminescence (ECL) method was developed for the determination of azithromycin based on the sensitizing effect of azithromycin on Ru(bpy)32+. Moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the Ru(bpy)32+ system and the Ru(bpy)32+- azithromycin system were investigated by ECL respectively. The results show that the proposed electrode showed very good electrochemical activity and electrochemiluminescence response to the Ru(bpy)32+- azithromycin system, under the optimum experimental condition, the linearity of azithromycin concentration to ECL intensity was achieved in the concentration of azithromycin from 1.0 × 10−7 to 1.0 × 10−4 mol L−1 and the detection limit (S/N = 3) was 1.33 × 10−9 mol L−1. Through the parallel determination of 1.0 × 10−5 mol L−1 azithromycin for 8 times, the RSD of results was 1.82%, and the recovery ranging from 97.9% to 101.6% was obtained in the detection of azithromycin tabelets and the RSD was 1.36% (n = 5). The proposed method showed high selectivity and sensitivity and simplicity, and it could be applied to the analysis of azithromycin with satisfactory results.
研究领域:
分析化学
·
药物分析
·
生物医学工程
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DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2021
·
IF 3.975
Hao Li,Weijun Zhen,Chengyuan Dong,Ling Zhao
Nano boron nitride-trimethylolpropane tris (3-mercaptopropionate) grafted poly (L-lactic acid) (BN-TTMP-PLLA) was prepared with nonmetallic organic catalyst by thiol-ene click chemistry. By orthogonal experiment and range analysis, the optimum reaction conditions of BN-TTMP-PLLA were as follows: reaction time of 2.5 h, photoinitiator addition amount of 0.04 g, TTMP addition amount of 0.9 g, and mass ratio of PLLA-AA to BN-570 of 1:1. The product was characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, which showed that PLLA was successfully grafted onto BN surface. Furthermore, PLA/BN-TTMP-PLLA nanocomposites were prepared by melt blending with BN-TTMP-PLLA as modifier to enhance the performance of PLA . Compared with pure PLA, the impact strength and elongation at break of PLA/BN-TTMP-PLLA (0.5 wt%) nanocomposites were increased by 1.58 times and 7.34 times, respectively. Molecular dynamics simulation calculation showed that E Interaction between PLA and BN before and after functionalization decreased from −177.44 kcal/mol to −516.42 kcal/mol, which proved that intermolecular interactions between them were strengthened. In addition, PLA/BN-TTMP-PLLA (0.5 wt%) nanocomposites during melting and isothermal crystallization were studied by Rheonaut technology for simultaneous rheometry and FTIR, which demonstrated that the crystallization properties of PLA was clearly improved due to the strong hydrogen bonding effect between BN-TTMP-PLLA and PLA.
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高影响因子
危险品化学品经营许可证（带存储）