Food Bioscience
·
2025
·
IF 5.9
Hai Chi,Yue Xiao,Xuefei Wen,Houqi Ning,Xingzhong Zhang,Hongrui Chen,Jie Tang
This study developed an electrochemical sensor incorporating a dual recognition strategy that integrates molecularly imprinted polymer (MIP) and aptamers for the ultra-sensitive detection of zearalenone (ZEN) in grains. Exploiting the high specific surface area of the Cu-Zr bimetallic metal-organic framework-818 (MOF-818), aptamers were covalently immobilized on the olefin-functionalized MOF-818 surface via thioenol reaction. Employing 3-thiopheneacetic acid functionalized gold nanoparticles (3-TAA@AuNPs) as functional monomers, nanoscale molecularly imprinted cavities were electro-polymerized onto the modified electrode surface in the presence of the ZEN template. This synergistic recognition system integrates the aptamer's specific binding capability with the spatial complementarity of MIP, displaying an excellent linear response over a concentration range of 10 −13 to 10 −5 g/L and a detection limit as low as 2.8 × 10 −14 g/L. Analysis of real samples exhibited ZEN recoveries ranging from 96.5 % to 105.0 % (RSD <5.2 %) in corn and wheat matrices, confirming its practical applicability for rapid screening of fungal toxins in agricultural commodities.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yifei He,Xiaoshuo Zhang,Lei Shi,Baizhao Zeng
Zearalenone (ZEN), a common mycotoxin, has significant threat to human and animal health, so its rapid and sensitive detection in foods is quite important. In this work, a magnetic molecular imprinting electrochemiluminescence (ECL) sensor was proposed for its detection, which was constructed by loading carbon dots (CDs) with Fe 3 O 4 magnetic nanoparticles (MNPs) and then coating them with a molecular imprinted polymer (MIP). The CDs were prepared using waste coffee grounds, enabling low-cost and large-scale production. After polyethyleneimine modification, the CDs could generate strong ECL signal in the presence of tri-n-propylamine. Through siloxane cross-linking, the CDs were anchored onto the MNPs surface, and the MIP was simultaneously formed. The resulting molecularly imprinted nanoparticle exhibited low cytotoxicity under tested conditions and was able to rapidly enrich and separate ZEN. The obtained sensor demonstrated high stability, sensitivity, reproducibility, selectivity, and anti-interference capability. Under optimized conditions, the ECL intensity was linear to the logarithm of ZEN concentration in the range of 1.0 × 10 −3 –3.2 × 10 2 ng mL. When applied to detect ZEN in grain samples (including corn, wheat flour, and rice), the spiked recoveries ranged from 90.1 % to 109 %, with relative standard deviation below 7.2 %. This work provides a novel approach for the low-cost and rapid detection of mycotoxins.
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高影响因子
Toxins
·
2024
·Vol. 16
·期 3
·121
·
IF 3.9
Hira Sayed,Qiongqiong Zhang,Yu Tang,Yanan Wang,Yongpeng Guo,Jianyun Zhang,Cheng Ji,Qiugang Ma,Lihong Zhao
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin found in many agricultural products and can cause reproductive disorders, mainly affecting spermatogenesis in male animals. Rutin (RUT) is a natural flavonoid compound recognized for its significant antioxidant, anti-inflammatory and estrogenic properties. The present study aimed to determine the protective role of RUT against ZEN-induced reproductive toxicity in male mice. Twenty-four adult Kunming male mice were divided into four groups: control, RUT (500 mg/kg RUT), ZEN (10 mg/kg ZEN), ZEN + RUT (500 mg/kg RUT + 10 mg/kg ZEN), with six replicates per treatment. The results indicated that RUT mitigated ZEN-induced disruption in spermatogenic cell arrangement, decreased spermatozoa count, and increased sperm mortality in the testes. RUT significantly restored ZEN-induced reduction in T, FSH, LH, and E2 serum levels. Moreover, RUT mitigated ZEN-induced apoptosis by increasing the mRNA expression level ofbcl-2, decreasing the mRNA expression level ofkiss1-r, and decreasing the protein expression level of caspase 8 in reproductive tissues. These findings indicate the protective role of RUT against ZEN-induced reproductive toxicity in male mice by regulating gonadotropin and testosterone secretions to maintain normal spermatogenesis via the HPG axis, which may provide a new application direction for RUT as a therapeutic agent to mitigate ZEN-induced reproductive toxicity.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 14
·2300112
·
IF 3.1
Zhuqiang Wu,Xiangqiong Jiang,Yanqun Yang,Rui Shi,Guihua Ruan,Yipeng Huang
In this work, amphiphilic polymers synthesized from carboxylated carbon nanotubes stabilized high internal phase emulsions are demonstrated to be capable of direct extracting zearalenone and zearalanone in samples consisting of an oil-water emulsion system. Under optimal conditions, the maximum adsorption capacities for zearalenone and zearalanone are 17.27 and 13.26 mg/g. The adsorption is mainly attributed to π-π interaction, hydrophobic interaction, and hydrogen-bonding interaction for zearalenone and zearalanone. The adsorption isotherms reveal that the adsorption of zearalenone and zearalanone on amphiphilic polymers synthesized from carboxylated carbon nanotubes stabilized high internal phase emulsions follows Freundlich model with multilayer and heterogeneous adsorption due to the presence of multiple kinds of adsorption sites. The relative recoveries of the spiked zearalenone and zearalanone in corn juice samples range from 85% to 93% with relative standard deviations lower than 3.52%. The results manifest the high efficiency of amphiphilic polymers synthesized from carboxylated carbon nanotubes stabilized high internal phase emulsions for the adsorption and separation of analytes in the oil-water emulsion system. This study provides a new perspective on adsorbent engineering for the adsorption application in heterogeneous media.
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高影响因子
FOOD CONTROL
·
2017
·
IF 3.496
Zheng Han,Keqiu Jiang,Zhichen Fan,José Diana Di Mavungu,Maofeng Dong,Wenbo Guo,Kai Fan,Katrina Campbell,Zhihui Zhao,Yongjiang Wu
A simple and rapid magnetic solid-phase extraction (M-SPE) procedure using multi-walled carbon nanotube-magnetic nanoparticles (MWCNT-MNPs) as sorbents was established for purification of zearalenone (ZEA), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalanone (ZAN), α-zearalanol (α-ZAL) and β-zearalanol (β-ZAL) in maize. The main parameters affecting the clean-up efficiency were thoroughly investigated, and high purification efficiencies for all analytes were obtained. The resulting MWCNT-MNP-ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was validated for maize samples. The matrix effects were greatly minimized using the M-SPE approach, with signal suppression/enhancement values decreased from 69.9–127.6% to 92.1–103.8%. Consequently, complex matrix-matched calibration curves were not necessary and the calibrations constructed in acetonitrile could be applied for accurate quantification of the targeted mycotoxins in real samples. The average recoveries ranged from 75.8 to 104.1% and the inter- and intra-day precision values expressed as RSDs, were all lower than 14%. Limits of detection and quantification were in the range of 0.03–0.04 and 0.07–0.10 μg/kg, respectively. The analytical performance of the developed method was also successfully evaluated with maize samples, and this method was proved to be a powerful tool for monitoring ZEA and its derivatives in maize.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jingjing Wang,Pingping Qu,Fuhan Wang,Man Qian,Ziyi Li,Mengyao Guo,Xuejiao Gao
Zearalenone is an estrogen-like mycotoxin produced by Fusarium fungi. Growing evidence shows that ZEA causes multisystem damage via oxidative stress. Glycyrrhizic acid (GA), from Glycyrrhiza glabra, has anti-inflammatory/antioxidant properties. The mechanisms of GA and ZEA in chicken pulmonary oxidative stress and inflammation remain elusive. Therefore, we established zearalenone-exposed chicken lung and CP-II cells models. ZEA exposure triggers an inflammatory cascade via the ROS-NF-κB axis, activating NLRP3 inflammasomes and promoting the release of proinflammatory factors. It also increased the apoptosis-related factors and fibrosis-related factors. GA treatment reduced oxidative stress, inflammation, and apoptosis, while suppressing fibrosis. This study reveals that GA reverses lung tissue injury by suppressing oxidative stress, inflammation, and the apoptosis-necrosis network through regulating the NF-κB/NLRP3 signaling axis by scavenging ZEA-induced reactive oxygen species (ROS). These results elucidate the antioxidant mechanism of GA and provide insights into the toxicology of ZEA.
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Dan Wei,Jianliang Li,Ming Guo,Jingjing Xu,Qiao Deng,Xu Wang
Aflatoxins, zearalenone and its metabolites, as representative hazard mycotoxins cause adverse effects on food safety and human health. Developing a sensitive and reliable extraction and detection method is of great importance for monitoring their residue and exposure levels. In contrast to traditional trial-and-error selection steps, 4,4′,4″-(1,3,5-triazine-2,4,6-triyl) trianiline covalent-bonding with 2,5-dihydroxyterephthalaldehyde, namely TAPT-OH-COF was screened as a potential adsorbent utilizing density functional theory calculations prior to the synthesis procedure. After experimental verification, magnetic TAPT-OH-COFs were prepared, characterized and applied for the extraction of aflatoxins, zearalenone and its metabolites from food and biological samples, coupled with high-performance liquid chromatography tandem mass spectrophy detection. Under the optimal conditions, the developed method exhibited low limits of quantification (0.05–0.50 μg/kg), satisfactory recoveries (75.8 %–110.9 %) and good precision with intraday and interday relative standard deviations (RSDs) not exceeding 12.2 %. This study may provide great potential for the selection of candidate adsorbents for multi-mycotoxins extraction from complex samples.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Keqiu Jiang,Peng Huang,Lianjun Luan,Kai Fan,Wenbo Guo,Zhihui Zhao,Yongjiang Wu,Zheng Han
For the first time, a reliable solid-phase extraction (SPE) procedure using iron (II, III) oxide (Fe 3 O 4 )/multi-walled carbon nanotube (MWCNT) composite as sorbents was proposed for purification and enrichment of zearalenone (ZEA) and four type A trichothecenes (T-2 toxin (T-2), HT-2 toxin (HT-2), neosolaniol (NEO) and diacetoxyscirpenol (DAS)) in Salviae miltiorrhiza Radix et Rhizoma (Danshen). The Fe 3 O 4 /MWCNT composite was synthesized by assembling Fe 3 O 4 with MWCNT by sonication through an “aggregation wrap” mechanism and several key parameters affecting the performance of SPE procedure such as the composition of sample loading solutions, washing and elution solvents were thoroughly investigated. After optimization, 2% acetonitrile aqueous solution as the loading solution, 5% methanol aqueous solution as the washing solution and acetone containing 0.5% formic acid as the elution solvent presented an excellent purification efficiency for the five targets in Danshen. Under the optimal sample pretreatment conditions followed by analysis with ultra-high performance liquid chromatography-tandem mass spectrometry, satisfactory linearity (R 2 ≥ 0.991), high sensitivity (limit of quantification in the range of 1.20–4.80 μg kg −1 ), good recovery (73.7–91.9%) and acceptable precision (RSD, 2.1–13.3%) were obtained. The applicability of the validated method was further verified in real Salviae miltiorrhiza Radix et Rhizoma samples.
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高影响因子
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