INDUSTRIAL CROPS AND PRODUCTS
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2019
·
IF 4.191
Liangfan Qu,Guiyun Chen,Shuang Dong,Yaoxing Huo,Zhonglin Yin,Shuhong Li,Ye Chen
Highly dispersible TiO 2 nanoparticles were prepared through the hydrolysis of titanium alcohol, and a series of TiO 2 -modified zein/chitosan (CS) films were prepared through a process involving blending, solution casting and evaporation. The mechanical properties and the antibacterial capacity of the zein/CS/TiO 2 films were characterized to explore the effects of different TiO 2 nanoparticle contents. Results showed that the addition of nanoscale TiO 2 remarkably improved the mechanical properties and thermal stability of the zein/CS/TiO 2 film. The tensile strength of the zein/CS/TiO 2 films significantly increased (p<0.05) with the increasing TiO 2 loadings in films, reaching a maximum of 28.33 ± 1.53 MPa at 0.15 wt % TiO 2 nanoparticles. The antibacterial effect of the composite films on Gram-positive bacteria ( S. aureus ) was more significant than that of Gram-negative bacteria ( E. coli and S. enteritidis ), and the antibacterial effect of UV light was better than that of dark conditions. In particular, the inhibition zones of zein/CS/TiO 2 (0.15%) film against E. coli , S. enteritidis and S. aureus under UV light condition increased by 21.78%, 21.45%, and 26.44%, respectively, compared with the control. This study suggested that adding highly dispersible TiO 2 nanoparticles is an effective method for improving the mechanical and antimicrobial properties of zein/CS films for various applications, such as medical and food packaging.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 54
·期 11
·8591-8601
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IF 3.442
Huang Wenquan,Li Shaomin,Li Zhixian,Zhu Wei,Lu Shan,Jiang Yanbin
The development of multi-functional nanoparticles is important in overcoming the poor stability and low mucus permeation of traditional drug delivery systems. Hence, the encapsulation of a model drug, resveratrol (RES), into zein–polydopamine–lecithin (ZPLs) nanoparticles was investigated through a delicately controlled phase separation process. The surface of the nanoparticles obtained was successfully modified with lecithin, which endowed them with neutral and hydrophilic properties. Due to the strong charge repulsion of the particles, the fabricated ZPLs showed high stability under the conditions of a saline solution, various pH, long-term storage and high-speed centrifugation. In vitro study suggested that the RES exhibited remarkably sustained release performance after loading into ZPLs. The quantificational experiment of the Transwell system suggested that most of the ZPLs could pass through mucus and exhibited high mucus permeation. This study provides a new opportunity to prepare RES-ZPLs nanospheres as a smart drug nanocarrier, with enhanced stability and mucus permeation.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Chong Li,Kao Wu,Yuehong Su,Saffa B. Riffat,Xuewen Ni,Fatang Jiang
Konjac glucomannan (KGM)/zein blend films were successfully prepared by solution casting at different drying temperatures (40, 50, 60, 70 and 80 °C). The effects of drying temperature on the films' structural, thermomechanical, mechanical and water barrier properties were investigated. Microstructural observations indicated that zein particles were homogeneously dispersed in KGM continuous matrix, and the blend film dried at 60 °C showed the most compact and smooth surface. Dynamic mechanical thermal analysis curves showed that with increasing drying temperature from 40 to 60 °C, glass transition temperature (Tg) of films increased; however, with further increase in temperature, the Tg decreased, indicating the compatibility of film components was the highest when dried at 60 °C. The hydrophobicity of blend film dried at 60 °C was significantly stronger than that of other blend films, supported by the highest water contact angle, and the lowest swelling ratio and solubility. Moreover, the film dried at 60 °C showed the highest tensile strength , elongation at break, and the lowest water vapor permeability. Therefore 60 °C was preferred for KGM/zein blend film preparation. This study indicated that intermolecular interactions among film components were greatly influenced by the drying temperature, and should be carefully noticed for film preparation.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 21
·14907-14921
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IF 2.993
Pang Jiafeng,Li Zhixian,Li Shaomin,Lin Shuying,Wang Hongdi,Xie Qiuling,Jiang Yanbin
Integrating various functional components into a single nano-platform is an ideal but challenging strategy for cancer therapy. Herein, a facile approach to fabricating multi-targeted nano-drug delivery systems which can be monitored is reported. Superparamagnetic iron oxide nanoparticles (SPIONs) and gefitinib (GEF) were encapsulated into folic acid-conjugated zein (Fa–zein) nanocomplexes (GEF-FSZs) with good dispersity, high GEF loading efficiency and pH-dependent release profile. The uptake of water-insoluble GEF was facilitated by encapsulating GEF into a zein-based nanocomplex, the magnetic responsive property endowed by SPIONs and the conjugation of Fa, which resulted in enhanced toxicity to A549 cells. The endocytosis study indicated that macropinocytosis and clathrin/caveolae-independent endocytosis exerted great influence on the internalization of GEF-FSZs. These results implied that GEF-FSZs could be a promising candidate for controlled and targeted drug delivery.
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高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Xiaofeng Ren,Ting Hou,Qiufang Liang,Xi Zhang,Di Hu,Baoguo Xu,Xinxiang Chen,Meram Chalamaiah,Haile Ma
In this study, resveratrol was successfully encapsulated using zein-chitosan complex coacervation. The encapsulation efficiency was markedly improved (51.4%) after chitosan coating at 1:2.5 zein/chitosan ratio, compared with 38.6% using native zein . Analysis of multi-model frequency ultrasound treatment effects on resveratrol encapsulation using zein-chitosan complex coacervation showed that 28/40 kHz dual-frequency ultrasound led to the highest encapsulation efficiency (65.2%; 31.9% increase) and loading capacity (5.9%; 31.1% increase) of resveratrol, followed by multi-frequency ultrasound at 20/28/40 kHz (17.8% encapsulation efficiency increase; 17.8% loading capacity increase). Dual-frequency ultrasound treatment significantly reduced the zein-chitosan complex coacervation particle size and reduced their distribution, however, did not change the zeta potential. Fourier transform infrared spectroscopy and fluorescence spectroscopy analysis demonstrated that ultrasound treatment had no effect on secondary structure of zein-chitosan complex but markedly decreased the fluorescence emission intensity. Differential scanning calorimetry and X-ray diffraction results indicated that Dual-frequency ultrasound treatment improved the thermal stability of zein-chitosan complex coacervation but had no effect on the crystal structure. Atomic force microscopy and scanning electron microscopy images revealed uniform distribution of zein-chitosan complex coacervation followed by ultrasonic treatment.
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2024
·
IF 4.7
Kumar Ganesan,Cong Xu,Chunguang Xie,Yue Sui,Chuan Zheng,Fei Gao,Jianping Chen
Breast cancer (BC) is the most common cancer in women and is known for its tendency to spread to the bones, causing significant health issues and mortality. In this study, we aimed to investigate whether cryoprotective isoliquiritigenin-zein phosphatidylcholine nanoparticles (ISL@ZLH NPs) could inhibit BC-induced bone destruction and tumor metastasis in both in vitro and animal models. To evaluate the potential of ISL@ZLH NPs, we conducted various experiments. First, we assessed cell viability, colony formation, transwell migration, and wound healing assays to determine the impact of ISL@ZLH NPs on BC cell behavior. Western blotting, TRAP staining and ALP activity were performed to examine the effects of ISL@ZLH NPs on osteoclast formation induced by MDA-MB-231 cell-conditioned medium and RANKL treated RAW 264.7 cells. Furthermore, we assessed the therapeutic impact of ISL@ZLH NPs on tumor-induced bone destruction using a mouse model of BC bone metastasis. Treatment with ISL@ZLH NPs effectively suppressed BC cell proliferation, colony formation, and motility, reducing their ability to metastasize. ISL@ZLH NPs significantly inhibited osteoclast formation and the expression of factors associated with bone destruction in BC cells. Additionally, ISL@ZLH NPs suppressed JAK-STAT signaling in RAW264.7 cells. In the BCBM mouse model, ISL@ZLH NPs led to a significant reduction in osteolytic bone lesions compared to the control group. Histological analysis and TRAP staining confirmed that ISL@ZLH NPs preserved the integrity of bone structure, preventing invasive metastasis by confining tumor growth to the bone marrow cavity. Furthermore, ISL@ZLH NPs effectively suppressed tumor-induced osteoclastogenesis, a key process in BC-related bone destruction. Our findings demonstrate that ISL@ZLH NPs have the potential to inhibit BC-induced bone destruction and tumor metastasis by targeting JAK-STAT signaling pathways and suppressing tumor-induced osteoclastogenesis. These results underscore the therapeutic promise of ISL@ZLH NPs in managing BC metastasis to the bones.
研究领域:
肿瘤学
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药理学
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纳米技术
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细胞生物学
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
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IF 5.4
Mingfang Sun,Jun Wang,Jun Kang,Yaxin Zhao,Huafeng Tian,Yujuan Jin,Rakesh Kumar,Junfeng Wang
This study utilizes electrospinning to develop bio-based fibrous films composed of zein and polylactic acid (PLA), with chitosan added in varying concentrations. The resulting films exhibit remarkable filtration efficiency, capturing over 95 % of airborne pollutants, while maintaining a low pressure drop of less than 4 mbar. A key innovation in this work is the integration of chitosan. Chitosan, a naturally derived polysaccharide, significantly increases the conductivity and viscosity of the spinning solution, enhancing interactions among PLA, chitosan, and protein molecules. This leads to a robust network structure with improved hydrophobicity and a bimodal structure, both essential for maintaining high filtration efficiency without reducing breathability. The addition of chitosan effectively addresses the common issue of high pressure drop in PLA/zein composite films, improving the product's efficiency and practicality for air filtration applications. Furthermore, the inherent mechanical strength and ductility of chitosan contribute to the fibrous films' overall durability and functionality. These properties make chitosan-modified zein/PLA fibers highly suitable for air pollution control, especially in personal protective equipment. This research underscores the potential of chitosan-enhanced bio-based materials as sustainable and effective solutions for advanced air filtration technologies.
研究领域:
材料科学
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环境科学
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生物工程
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JOURNAL OF FOOD ENGINEERING
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2016
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IF 3.199
Jiahui Gu,Zhong Xin,Xin Meng,Shuzhen Sun,Qingan Qiao,Hongbo Deng
In this study, the zein extracted from distillers dried grains with solubles (DDGS) is utilized to produce a fat analogue, by employing a simple ”reduced-pressure distillation microparticulation“ method. The optimal conditions for preparing the fat analogue are as follows: a zein concentration of 3 w/v% (based on 60 v/v% ethanol water), an agitator speed of 200 rpm, a xanthan gum amount of 20 wt% (based on zein mass) and a heating temperature of 50 °C. The zein-based fat analogue obtained has a volume-weighted mean diameter (D[4, 3]) of about 16 μm, which is then applied in the formulation of mayonnaise , at fat substitution ratios of 0% (full-fat), 20%, 40%, 60%, 80% and 100%, respectively. Based on the appearance, stability, total calorific value, as well as rheological, microstructure and sensory analyses, it may be concluded that the fat substitution ratio of no more than 40% is acceptable. This paper illustrates another high-value added use of zein.
研究领域:
食品科学
·
生物化学
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高影响因子
FOOD CHEMISTRY
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2018
·
IF 4.946
Yike Yue,Sheng Geng,Yue Shi,Guizhao Liang,Jinshui Wang,Benguo Liu
Zein has the potential application of establishing the delivery systems for flavonoids . But there are few reports about the effect of the molecular structures of flavonoids on their interaction with zein . In this study, the binding behaviour of 21 flavonoids and zein was investigated by spectrofluorimetry. The corresponding 3D-QSAR model was also established by Topomer CoMFA method, whose steric and electrostatic field analysis could explain the binding performance of the tested flavonoids with zein. The fluorescence analysis suggested that the flavonoids could interact with zein by forming the complex at the molar ratio of 1. The flavonoids with glucosyl groups at ring A exhibited the outstanding binding capacity with zein, and their binding process with zein was driven by hydrophobic force. The synchronous and 3D fluorescence spectra showed that there was no apparent change in the microenvironment surrounding the tyrosine residues of zein during the interaction.
研究领域:
食品科学
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生物化学
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Polymers
·
2021
·Vol. 13
·期 16
·2759
·
IF 4.329
Longwei Jiang,Yanlong Han,Xiangyi Meng,Yawen Xiao,Huajiang Zhang
In this study, following the green, environmentally friendly and sustainable development strategy, cellulose nanocrystals (CNCs) were prepared through a solvent-free esterification reaction between microcrystalline cellulose and maleic anhydride, combined with subsequent ultrasonic treatment, and maleic-anhydride-modified CNC-reinforced zein/catechin/β-cyclodextrin inclusion complex nanoparticles nanocomposite films were prepared by a facile solution casting. The amount of CNCs in the film matrix was 0–8 wt%, and their effect on structural, physicochemical and functional properties of the resulting films were investigated. SEM images showed that the addition of CNCs made the microstructure of the film more smooth and uniform. The intermolecular hydrogen bonds between CNCs and film matrix were supported by FT-IR. XRD analysis also confirmed the appearance of a crystalline peak due to the existence of CNCs inside the films. The incorporation of CNCs significantly reduced water vapor permeability, water solubility and the swelling degree of the nanocomposite film, and also significantly increased tensile strength and elongation at break from 12.66 to 37.82 MPa and 4.5% to 5.2% (p< 0.05). Moreover, nanocomposite film packaging with CNCs can effectively inhibit the oxidation of soybean oil.Keywords:cellulose nanocrystals;zein;antioxidant activity;biodegradable films;food oxidation
研究领域:
材料科学
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食品科学
·
纳米技术
·
可持续发展
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jin Chen,Zhuangwei Zhang,Huihui Li,Mengchu Sun,Hongjin Tang
The low water solubility and inadequate bioavailability of curcumin significantly hinder its broad biological applications in the realms of food and medicine. There is limited information currently available regarding the particle characteristics and functional capabilities of zein-lysozyme-based nanomaterials . Thereby, the primary goal of the current work is to effectively develop innovative zein-lysozyme- κ -carrageenan complex nanocomposites (ZLKC) as a reliable carrier for curcumin encapsulation. As a result, ZLKC nanoparticles showed a smooth spherical nanostructure with improved encapsulation efficiency. Fourier-transform infrared, fluorescence spectroscopy, dissociation assay, and circular dichroism analysis revealed that hydrophobic and electrostatic interactions and hydrogen bonding were pivotal in the construction and durability of these composites. X-ray diffraction examination affirmed the lack of crystallinity in curcumin encapsulated within nanoparticles . The incorporation of κ -carrageenan significantly improved the physicochemical stability of ZLKC nanoparticles in diverse environmental settings. Additionally, ZLKC nanocomposites demonstrated enhanced antioxidant and antimicrobial properties, as well as sustained release characteristics. Therefore, these findings demonstrate the potential application of ZLKC nanocomposites as delivery materials for encapsulating bioactive substances.
研究领域:
纳米技术
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食品科学
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药学
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材料科学
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 10
·6707-6719
·
IF 3.2
Qilei Wang,Zhenzhen Feng,Chan Shi,Xingang Lyu,DaiDi Fan
Sulforaphane-loaded nanoparticles (NP-SF) were prepared in this study to improve their biological effects. Based on propylene glycol alginate and zein as wall materials and anthocyanin and CaCl 2 as crosslinking agents, the NPs were encapsulated by the crosslinking method and freeze-dried. With the increasing contents of anthocyanin and Ca 2+ , the encapsulation efficiency and loading capacity of NP-SF were both increased. In vitro simulated digestion experiments showed controlled release of SF from the NPs. The pharmacokinetics confirmed that NP-SF exerted a slower release effect in rats, with improved SF bioavailability and protective effects on liver injury induced by N -diethylnitrosamine in mice. NP-SF reduced serum indicators of liver injury, increased the activities of antioxidant enzymes and GSH levels, and reduced malondialdehyde levels in the liver. In addition, SF activated the Keap1/Nrf2 signaling pathway and upregulated the expression of the Nrf2 downstream genes NQO1 and heme oxidase 1. High doses of NP-SF, in particular, had a higher therapeutic effect. In conclusion, encapsulation enhanced the biological activity of SF and promoted physiological function.
研究领域:
药理学
·
肝脏病学
·
食品科学
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高影响因子
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