Frontiers in Pharmacology
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2026
·
IF 5.4
Yuan Wei,Yanhuan Yu,Yuning Zhu,Xinwen Zhang,Zhuoling Zhao,Sinan Wang,Fei Fei,Bei Cao,Runbin Sun,Juan Li
Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma (NHL), with an increasing incidence in recent years. Ouabain, a cardiotonic steroid, has demonstrated anti-tumor effects in various cancers including DLBCL, but its mechanism of action is not yet fully understood. We first used liquid chromatography-tandem mass spectrometry (LC-MS) for proteomics and metabolomics to analyze changes in DLBCL cells with ouabain administration. The results revealed significant alterations in the abundance of 303 proteins and 206 metabolites between the ouabain-treated and control groups. Proteins like MYC, RAD51, AURKB, CENPF, and RRM2 were downregulated, along with metabolism-related proteins ARG2, OAT, and TYMS. Metabolite levels including glutamate, glutathione, ornithine, and taurine were significantly altered. The integrated analysis revealed that arginine metabolism serves as a central hub, coordinating changes in glutathione metabolism and nucleotide biosynthesis, contributing to increased oxidative stress, replication stress, and growth inhibition. These findings suggest that ouabain disrupts arginine-driven metabolic network to alter redox balance and inhibit tumor cell proliferation, providing a promising therapeutic rationale for targeting metabolic vulnerabilities in DLBCL.
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EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Ze Xu,Dan Shou,Wenrong Cai,Junyao Li,Datong Wu,Tao Jiang,Yong Kong
6-Deoxy-6-arginine grafted chitosan (DAC) is cross-linked with dibenzaldehyde-functionalized polyethylene glycol (DF-PEG) via the acylhydrazone (–N=CH–) bonds, which can be utilized for the encapsulation of metformin (MET). The resultant injectable DAC/DF-PEG/MET (DDM) hydrogels highlight well-behaved antibacterial and anti-inflammatory activity as well as self-healing capability. More interestingly, the DDM hydrogels exhibit appealing dynamic self-degradability. The electrostatic attraction between DAC and DF-PEG is significantly weakened at slightly alkaline surface (pH 7.0–8.0) that is the characteristic of skin wounds, resulting in the swelling of the hydrogels and the release of MET. As the wounds gradually get healed, the skin tissue turns to be weakly acidic (pH 4.0–6.0), leading to the hydrolysis of the –N=CH– bonds between DAC and DF-PEG. Therefore, the DDM hydrogels can be dynamically self-degraded during the wound healing process. Owing to the injectability and self-healing properties, the DDM hydrogels are suitable for use as the wound dressing for the wounds in any shape. The developed DDM hydrogels also display excellent hemocompatibility and cytocompatibility, indicating that they might be a potential wound dressing for skin wounds.
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ACS Catalysis
·
2022
·
IF 13.7
Shuchao Jiang,Junxia Yang,Shengliang Zhai,Ling Zhang,Rui Tu,Tie Yu,Dong Zhai,Lei Sun,Weiqiao Deng,Guoqing Ren
Formic acid (FA)/formate as a promising liquid organic hydrogen carrier exhibits great potential for future energy supplies. However, FA/formate-based hydrogen storage and release, which can be operated under ambient conditions, is challenging but attractive. Here, we report an ambient hydrogen storage and release process that uses the acidity and alkalinity of the reaction solution to control hydrogen production or consumption via an l-arginine-functionalized active carbon-supported PdAu alloy catalyst (PdAu/AC-LA). By regulating the LA loading and Pd/Au ratio, we obtain the optimal catalytic activity with turnover frequency (TOF) of 1760 h–1 for FA dehydrogenation under acidic conditions and 138 h–1 for CO2 hydrogenation under alkaline conditions. Comprehensive characterizations, including X-ray photoelectron spectroscopy (XPS), CO2 temperature-programmed desorption (CO2-TPD), and HCOO– adsorption experiments, reveal that the synergistic effect between PdAu alloy and strongly basic l-arginine enhances catalytic activity by regulating the adsorption of reactants. Our work, as a successful example of FA/formate-based hydrogen storage and release under ambient conditions, may aid putting the safe and energy-saving use of hydrogen into practice.
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MICROSCOPY RESEARCH AND TECHNIQUE
·
2021
·Vol. 85
·期 4
·1518-1526
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IF 2.769
Leilei Hong,Lichan Yuan,Xiaoxu Xu,Yuhuan Ma,Li Meng,Junyi Wang,Na Zhao,Xiaoliang Wang,Junqing Ma
Titanium (Ti) alloys, particularly Ti 6 Al 4 V, are the most commonly used biomedical implant material. Ti alloys are biologically inert, so there have been continuous efforts to improve their osteogenic properties and clinical performance. Since TiO 2 nanotubes (NT) appear to be excellent drug platforms, and strontium reportedly enhances osteogenesis, we constructed a TiO 2 nanotube coating on the surface of Ti 6 Al 4 V and immersed it in Sr (OH) 2 solution in order to incorporate Sr into TiO 2 nanotubes (NT-Sr). The results of field emission scanning electron microscope and X-ray diffraction analysis verified the fabrication of NT-Sr. We next added polydopamine (PDA) and cyclo- (arginine–glycine–aspartic acid–phenylalanine–cysteine) [c(RGDfC)] peptides to further promote biocompatibility of the implant. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirmed the existence of PDA and c(RGDfC). Mesenchymal stem cells (MSCs) were planted on Ti, NT, NT-Sr, NT-Sr/PDA, and NT-Sr/PDA-RGD surfaces. The adhesion and differentiation of MSCs on different surfaces were evaluated. The mRNA expression of alkaline phosphatase, runt-related transcription factor 2 (Runx2) and type I collagen (Col I) of different groups were also tested. Finally, we observed that the NT-Sr/PDA-RGD group showed significantly better performance than other groups in terms of the differentiation and osteogenesis-related gene expression of MSCs. Thus, the NT-Sr/PDA-RGD complex may be an important modification strategy for Ti, as it shows excellent osteogenic potential.
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2019
·Vol. 85
·期 23
·
IF 4.077
Bingbing Ma,Qianwen Wang,Haruo Ikeda,Chunfang Zhang,Lian-Hua Xu
Our previous study showed that CYP105D7, a substrate-promiscuous P450, catalyzes the hydroxylation of 1-deoxypentalenic acid, diclofenac, naringenin, and compactin. In this study, 14 steroid compounds were screened using recombinant Escherichia coli cells harboring genes encoding CYP105D7 and redox partners (Pdx/Pdr, RhFRED, and FdxH/FprD), and the screening identified steroid A-ring 2β- and D-ring 16β-hydroxylation activity. Wild-type CYP105D7 was able to catalyze the hydroxylation of five steroids (testosterone, progesterone, 4-androstene-3,17-dione, adrenosterone, and cortisone) with low (<10%) conversion rates. Structure-guided site-directed mutagenesis of arginine residues around the substrate entrance and active site showed that the R70A and R190A single mutants and an R70A/R190A double mutant exhibited greatly enhanced conversion rates for steroid hydroxylation. For the conversion of testosterone in particular, the R70A/R190A mutant's kcat/Km values increased 1.35-fold and the in vivo conversion rates increased significantly by almost 9-fold with high regio- and stereoselectivity. Molecular docking analysis revealed that when Arg70 and Arg190 were replaced with alanine, the volume of the substrate access and binding pocket increased 1.08-fold, which might facilitate improvement of the hydroxylation efficiency of steroids.IMPORTANCE Cytochrome P450 monooxygenases (P450s) are able to introduce oxygen atoms into nonreactive hydrocarbon compounds under mild conditions, thereby offering significant advantages compared to chemical catalysts. Promiscuous P450s with broad substrate specificity and reaction diversity have significant potential for applications in various fields, including synthetic biology. The study of the function, molecular mechanisms, and rational engineering of substrate-promiscuous P450s from microbial sources is important to fulfill this potential. Here, we present a microbial substrate-promiscuous P450, CYP105D7, which can catalyze hydroxylation of steroids. The loss of the bulky side chains of Arg70 and Arg190 in the active site and substrate entrance resulted in an up to 9-fold increase in the substrate conversion rate. These findings will support future rational and semirational engineering of P450s for applications as biocatalysts.
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THERIOGENOLOGY
·
2026
·
IF 2.9
Kun Wu,Chenglong Yu,Guangmang Liu,Yong Zhuo,Bin Feng,Lianqiang Che,Shengyu Xu,Lun Hua,Jian Li,Zhengfeng Fang,De Wu,Yan Lin
Heat stress can severely impair male reproductive performance. Endoplasmic reticulum stress (ERS) is recognized as an early cellular response to stress, which can subsequently induce inflammation and apoptosis, thereby reducing semen quality. In this study, arginine, putrescine, and 4-phenylbutyric acid (4-PBA; an ERS inhibitor) were administered by gavage to a heat-stressed ICR mouse model subjected to seven days of heat exposure, in order to evaluate their effects on testicular function and the underlying mechanisms. Supplementation with arginine, putrescine, or 4-PBA significantly ameliorated heat stress-induced testicular damage and the decline in sperm quality in male mice. Specifically, the mRNA expression of Acrv1 , Izumo3 , and Tjp1 was markedly upregulated. In contrast, the number of TUNEL-positive testicular cells was significantly reduced, while the mRNA expression of apoptotic markers Bax and Caspase12 was downregulated. Moreover, the expression of ERS- and inflammation-related genes ( Grp78 , Perk , IRE1 , XBP1s , TLR4 , and NF-κB ) was significantly decreased. In conclusion, heat stress induces testicular dysfunction and inflammation. Both arginine and its metabolite putrescine alleviate heat stress-induced testicular impairment by reducing ERS and inflammatory responses, with arginine showing stronger protective effects than putrescine.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Li Hao,Minyan Zhou,Jialin Zeng,Jiechun Lin,Lan Hua,Ziting Yin,Mei Guan,Qiangcheng Zheng,Hongjun Zhou,Xinhua Zhou
To achieve the visualization of pesticides within plants, the development of fluorescent carriers is particularly important. Herein, arginine (Arg) was applied as a bridge agent to simultaneously exfoliate and functionalize boron nitride nanosheets (BNNS) via a mechanochemistry approach. Subsequently, phosphomolybdic acid (PMA) was grafted onto the structure to facilitate in-plant visualization and supply plant nutrients, yielding a BNNS-Arg-PMA carrier with a PMA content of 29.02 %. Compared to pristine h-BN, the resulting carrier exhibits a smaller average size of approximately 297 nm and a specific surface area increased to 57.017 m 2 ·g −1 . Furthermore, the abamectin (AVM) loading capacity of BNNS-Arg-PMA reaches 175.9 mg·g −1 , and the carrier significantly slows the release rate of AVM. It also effectively reduces the phytotoxicity of AVM on seed germination, demonstrating enhanced biosafety. Additionally, BNNS-Arg-PMA promotes plant growth, with markedly increased contents of nutrients phosphorus (P) and molybdenum (Mo), rising from original values of 0.316 % and 0.001 % to 2.484 % and 0.598 %, respectively. Moreover, BNNS-Arg-PMA exhibits stronger and more localized fluorescence compared to free PMA, allowing visual fluorescence-based tracking in the agricultural scene. The intrinsic fluorescence enables monitoring of pesticide carrier residues around rhizosphere without the need for external fluorescent labels.
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LANGMUIR
·
2026
·
IF 4.4
Yan Lu,Qianyun Ye,Can Gao,Xiaoge Li,Pan Gao,Xiaohuan Sun,Jie Han
The development of chiral type-I photosensitizers represents a significant advance, as it can not only overcome the hypoxia limitation inherent to conventional type-II agents but also leverage enantioselectivity to enhance therapeutic precision. However, this promising field remains notably underexplored. Herein, we construct chiral supramolecular self-assemblies via hydrogen-bonding interactions between l/d-arginine (l/d-Arg) and 5,10,15,20-tetrakis(4-sulfophenyl)porphyrin (TPPS). The proximity of the electron-donating porphyrin core to the arginine moiety facilitates efficient photoinduced electron transfer, enabling l/d-Arg-TPPS to operate via simultaneous type-I and type-II pathways. This dual mechanism ensures robust generation of superoxide radicals (O2•–) even under hypoxic conditions, addressing a key limitation of conventional porphyrin photosensitizers. Moreover, the self-assembly process drives effective supramolecular chirality induction, endowing TPPS with substantial dissymmetry factors (gabs) of ∼2.1 × 10–4. This chiral signature translates to significant biological enantioselectivity. d-Arg-TPPS achieves 3.87-fold greater cellular uptake and 1.85-fold higher intracellular reactive oxygen species (ROS) production than l-Arg-TPPS, resulting in markedly enhanced chirality-specific antitumor activity. Importantly, l/d-Arg-TPPS also elicits a chirality-dependent immunogenic cell death (ICD) response, facilitating antitumor immunity. This work establishes a supramolecular chiral-engineering strategy that integrates hypoxia-tolerant photodynamic behavior with stereoselective nanobio interactions, advancing both the fundamental understanding and therapeutic potential of photosensitizer design.
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Science Bulletin
·
2023
·
IF 18.9
Xiaoyong Yue,Liguang Xu,Hengwei Lin,Chuanlai Xu,Si Li
Chiral nanomaterials with different functions have been widely developed, but the deep understanding of the structural effects of nanocatalysts on enantioselective photocatalytic efficiency is still highly demanded. Herein, Pt and Pt-Au-bimetal-doped chiral nanostructures with various morphologies and compositions are facilely constructed using L- / D- arginine ( L- / D- Arg) and mono-sulfonate tetraphenyl porphyrin (H 2 TPPS) assemblies as chiral templates. Interestingly, these Pt and Pt-Au-doped chiral nanostructures, including nanorods (NR) and nanospheres (NS), can be well regulated by controlling pH, ionic strength , and reaction time of the assembling system of Arg and H 2 TPPS. More impressively, specific Au growth direction along the Pt-doped chiral NR ( L- / D- Pt-NR) is observed (from tip to middle) during the preparation of Pt-Au-bimetal-doped chiral NR ( L- / D- Pt-Au-NR) and their compositions can be finely controlled by simply adjusting the concentrations of HAuCl 4 . As expected, the chiral nanostructures exhibit superior enantioselective photocatalytic ability toward chiral organics under visible light: the oxidation rate of L- dihydroxy-phenylalanine ( L- DOPA) catalyzed by L- Pt-NR (or D- DOPA catalyzed by D- Pt-NR) is about 60% higher than that of L- DOPA catalyzed by D- Pt-NR (or L- DOPA catalyzed by D- Pt-NR). This study provides a facile strategy to construct chiral nanostructures for the photocatalytic conversion of chiral organics.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Peilong Wang,Xu Liu,Qingqing Hu,Hui Gao,Wei Ma
A simple and rapid electrochemical method based on a poly(L-arginine)-electrochemically reduced graphene oxide-modified glassy carbon electrode fabricated by cyclic voltammetry has been developed for the determination of tartrazine. Compared with the bare glassy carbon electrode, the modified electrode achieved a well-defined and enhanced oxidation peak due to the increased conductivity and electrochemical active surface area. The bare glassy carbon electrode and the modified electrode were characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The electrocatalytic oxidation behaviors of tartrazine on the modified electrode were investigated using cyclic voltammetry and differential pulse voltammetry. The voltammetric peak current of tartrazine exhibits good linearity in the range of 1.00×10 -6 -2.50×10 -4 mol L -1 under the optimal differential pulse voltammetry conditions, with a detection limit of 2.5×10 -7 mol L -1 (at an S/N of 3). This proposed method has also been applied for quantitative analysis of tartrazine in some carbonated beverage and fruit juice samples, with satisfactory results.
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JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2022
·Vol. 61
·期 3
·203-210
·
IF 1.555
Wang Ying,Li ZhiRong,Chen RongXiang
Arginine and its metabolites play important roles in pain and analgesia. This study aimed to develop a comprehensive quantification method for amino acids and metabolites related to arginine metabolism in rat plasma by hydrophilic interaction chromatography–tandem mass spectrometry (HILIC–MS/MS). Rat plasma was diluted to reduce the matrix effect and deproteinized with acetonitrile. The analytes were separated on a Syncronis HILIC column with a gradient elution. MS analysis was performed in positive ion mode with an electrospray ionization source using multiple reaction monitoring technology. All calibration curves for the 10 analytes showed good linear regression (R2 > 0.99). The limits of detection (LODs) were in the range of 0.9–13.4 μg/L. The established method was validated for intra-day and inter-day precisions (relative standard deviation [RSDs] < 6.21%) and accuracy (average recovery ranged from 87.34% to 100.35% with the RSD values less than 11.41%). This method was successfully applied to characterize dynamic alterations in the plasma of rats with neuropathic pain and thus provide service to explore the mechanism of action between metabolite changes and clinical disease.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Shuqing Xiang,Mingqian Wang,Li Li,Jian Shen
The gas therapy of some endogenous signaling molecules to treat diseases has caused extensive research, among which NO gas has shown great potential in fighting infection with various pathogens, promoting wound healing, etc. Here, we propose a photothermal/photodynamic/NO synergistic antibacterial nanoplatform by loading L -arginine (LA) on mesoporous TiO 2 and then encapsulating it with polydopamine . The obtained TiO 2−x [email protected] nanocomposite possesses both the excellent photothermal effect and ROS generation ability of mesoporous TiO 2 , and the release of nitric oxide (NO) from L -arginine under near-infrared (NIR) light irradiation , while the sealing layer of PDA could induce NIR-triggered NO controlled release. In vitro antibacterial experiments confirmed that the synergistic effect of TiO 2−x [email protected] nanocomposites has excellent antibacterial effects against Gram-negative and Gram-positive bacteria, while in vivo experiments showed that it has lower toxicity. It is worth noting that compared with the pure photothermal effect and ROS, the generated NO showed a better bactericidal effect , and NO had a better ability to promote wound healing. In conclusion, the developed TiO 2−x [email protected] nanoplatform can be used as a nanoantibacterial agent, which can be further explored in the related biomedical field of photothermal activation of multimodal combined antibacterial therapy.
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